ABSTRACT Romantic relationships both buffer and exacerbate adverse health outcomes among bisexual individuals, so understanding determinants of their functioning may be key to correcting health inequities affecting this population. Binegativity (i.e., prejudiced attitudes about bisexuality) expressed from one’s intimate partner has the potential to be uniquely impactful for bisexuals, but it is presently unknown how this experience might impact the quality (and therefore potential health promotive effects) of their romantic relationships. In addition, gender of one’s current romantic partner may shape experiences of binegativity and relationship quality in important ways. The purpose of this report was to examine how binegativity from intimate partners impacts relationship functioning (i.e., relationship satisfaction and intimate partner aggression) among bisexual men (N = 113), and how the gender of one’s partner might moderate this effect. Results suggested that binegativity is detrimentally associated with relationship satisfaction, and that bisexual men in relationships with women report more frequent intimate partner aggression. Furthermore, partner gender and binegativity showed novel interactive associations with intimate partner aggression, such that bisexual men in relationships with women tended to perpetrate less intimate partner aggression when experiencing more binegativity. These results are discussed with regard to future avenues for research into the health promotive benefits of relationships for bisexual individuals.