1-Methylcyclopropene, aminoethoxyvinylglycine, chlorine dioxide and nitric oxide were widely applied as ethylene synthesis inhibitors for crops at pre-harvest stages for various purposes. Durian (Durio zibethinus) was a seasonal fruit commonly cultivated in tropical regions. Durian was highly perishable at ambient condition due to its high respiration rate and ethylene accumulation. Preharvest treatment was considered as a promising strategy to regulate some physiological behaviors of durian during postharvest storage. This research was conducted during 2020 at Soc Trang Nanotech laboratory, Vietnam to evaluate the effectiveness of preharvest fumigation at dose 200 μL/L at 14 days before harvesting with 1-Methylcyclopropene (1-MCP), aminoethoxyvinylglycine (AVG), chlorine dioxide (ClO2) and nitric oxide (NO) on quality of durian fruit. After harvesting, the treated fruits were kept at 4°C for 28 days. In 7 day-interval, sample was taken to evaluate respiration rate, ascorbic acid content, glutathione content and ascorbic peroxidase activity. Results showed that 1-MCP-treated durian had the lowest respiration rate (101.24±1.27 mg CO2/kg/h) while maintained the highest ascorbic acid content (21.73±0.22 mg/100 g), glutathione content (29.30±0.11 μmol/kg) and ascorbic peroxidase activity (2.31±0.02 U/g) at the end of 28 days of storage. Preharvest treatment by 1-MCP significantly enhanced quality of durian fruit in an extended stability.