Preferred Variant Research Articles

МЕТОДИКА ОБОСНОВАНИЯ ХАРАКТЕРИСТИК ТЕПЛОИЗОЛЯЦИОННОГО МАТЕРИАЛА В СОСТАВЕ ОГРАЖДАЮЩЕЙ КОНСТРУКЦИИ С ИСПОЛЬЗОВАНИЕМ СРЕДСТВ КВАДРАТИЧНОГО ПРОГРАММИРОВАНИЯ

Abstract: Relevance of the research is connected to the importance of the problems related to the determination of the characteristics for structural solutions used in the construction or reconstruction of buildings and structures (taking into account the indicators of energy and economic efficiency), as well as the relatively low practical significance of the relevant scientific developments. Problem statement. It is necessary to determine the most preferred variant (model) of heat-insulating material used as an additional layer in the wall structure on the basis of the criteria related to the provided heat transfer resistance and the payback period of the corresponding design solution. Research aim. Development of tools for determination of the characteristics for thermal insulation material as part of wall structure within a residential building. Research tasks are the following: 1. Review and comparative analysis of scientific developments in the field of research. 2. Development of the procedure for determination of the characteristics for the heat-insulating material as part of the wall structure with the use of quadratic optimization tools. 3. Implementation of the developed procedure on a practical example. Results. Procedure for determination of the characteristics for the heat-insulating material as part of the wall structure with the use of quadratic optimization tools has been developed. The procedure has been implemented using a practical example, and the received results have been analyzed. Findings. The results of the procedure’s implementation on a practical example confirm the high practical significance of the developed tool. Thus, quadratic optimization tools can be effectively used to solve the problem related to the determination of the characteristics for heat-insulating material as part of an wall structure.

The effect of soil cultivation systems on agronomic and physical properties of dark gray soil and productivity of the field crop rotation link

The purpose of the research is to identify the most effective and preferable variant of the basic autumn tillage for inclusion of dark gray forest soil into the tillage system when sowing spring crops. The research was carried out in 2021–2023 in the conditions of the Chuvash Republic in the link of the field crop rotation "barley–peas–oats". Moldboard and non-moldboard autumn soil tillage methods were compared: plowing (26–28 cm) – control; cultivation (15–17 cm); disking (12–14 cm); without tillage. The tillage system was completed by surface early pre-sowing cultivation to a depth of 6 cm. During the studied years, the average soil density in the 0–30 cm layer was within the optimal values: after moldboard plowing it was 0.93–1.09 g/cm3 , after cultivation – 0.98–1.13 g/cm3 , without basic tillage– 1.05–1.20 g/cm3 . The average values of the soil porosity after plowing were in the range of 50.4–62.5 % and decreased with the abandonment of the basic tillage to 52.0–57.4 %. In arid conditions of 2021 growing season, the total supply of productive moisture in the 0–30 cm soil layer was 12–14 % higher after cultivation than after plowing. The analysis of the structural and aggregate state of the soil (0–20 cm) revealed an increase in the proportion of dusty particles by the end of the experiment after plowing from 3.9 to 6.9 %. When using a stubble cultivator as the basic tillage unit, the proportion of the lumpy fraction increased. The average annual productivity of the crop rotation link during moldboard plowing was the highest – 20.5 c/ha of grain units per 1 ha, after cultivation – 19.5 c of grain units per 1 ha. At the same time, plowing is the most expensive and economically unprofitable way of basic soil preparation. After cultivation with a KOS-3.0 combined stubble cultivator, the profitability of crop cultivation was significantly higher – 9.4–18.1 % after cereals in dry years and 96.9 % after peas under optimal hydrothermal conditions (for plowing – 8.3–4.2 % and 73.7 %, respectively).

Method for Determining the Conditions for Ensuring Stable Rearward Movement of a Semi-trailer Combination with Non-turning Semi-trailer Wheels

The efficiency of organising freight transport and using motor transport in a Smart City depends on a set of its properties, which in the process of operation determine its suitability for use in given operating conditions. Vehicles have different overall dimensions and designs, which determine their maneuverability and directional stability, their ability to make a curve-line movement in the city, as well as to move safely in reverse. Nowadays, tractor-trailer combinations with non-turning semi-trailer wheels are widely used for freight transport. Such a scheme of vehicle construction does not ensure its directional stability when reversing, which can lead to the folding of the tractor and semi-trailer and loss of mobility of the combination. In the absence of additional special systems, the driver controls the rearward movement of the road train using the steering wheel and rear-view mirrors, and the accuracy of delivery to the object depends on the level of the driver's training. The research of driver's reaction time spent on situation assessment, decision making, and realisation has been carried out. It has been revealed that with manual control, there are difficulties connected with training of drivers to estimate the parameters of movement and control of the road train, in particular, with the process of parking and its accurate delivery to the object. With the help of the developed mathematical model of the rearward movement of the road train and the proposed new turn control law, the research of maneuvering of the road train during its delivery to the object has been carried out and the results confirming the possibilities of building an automated system allowing to reduce the influence of the human factor on the parking process, as well as to reduce the time required to perform the maneuver have been obtained. The conducted analysis of system stability with the help of Routh–Hurwitz criterion has determined the conditions of providing stability of the system moving in reverse. The method of controlling the backward movement of the road train and the design of the device realising it has been developed. The results of simulation modeling allowed us to find the necessary values for the control law for the movement of road trains of different lengths, as well as the preferred variants of the initial positions of the road train for the realisation of its precise delivery to the object.

An Exploratory Study of Alternative Time Frames and Descriptors for EQ-5D-5L in Obstructive Airway Diseases Using Mixed Methods

ObjectivesEQ-5D-5L with its recall time of “today” may limit its ability to capture episodic symptoms and exacerbations in chronic obstructive airway diseases (OAD). We examined whether longer time frames and changing the intensity response scales to frequency scales could improve the measurement properties of EQ-5D-5L. MethodsWe used a mixed-method design starting with in-depth interviews with 20 patients and clinicians to elicit preferred time frames using concept elicitation techniques and content analyses. We then administered the top 4 preferred variants using 1- and 4-weeks’ time frames with the original intensity or an alternative frequency response scale alongside EQ-5D-5L and St George Respiratory Questionnaire to OAD patients during 2 different visits. We compared the ceiling effects and construct validity by testing a priori hypotheses in relation to St George Respiratory Questionnaire and clinical outcomes via correlation and receiver operating characteristic (ROC) analyses, respectively. Follow-up patients were categorized into “better,” “stable,” and “worse” groups to assess reliability using intraclass correlation coefficient (ICC) or Cohen’s Kappa (k) and responsiveness using ROC analysis. ResultsA total of 184 patients (mean [SD] age: 54[18]; female: 37.0%) completed baseline assessments. A total of 120 patients also completed follow-up assessments (mean [SD] interval: 2.8 [1.7] months). The ceilings were lower in the variants compared with EQ-5D-5L (P < .001). Reliability of the variants were comparable to or higher than EQ-5D-5L. The c-statistic values derived from ROC analyses of the variants were consistently higher than EQ-5D-5L. ConclusionsUse of longer time frames with the original intensity or the frequency response scales may improve EQ-5D-5L’s psychometric properties in OAD patients.

Effect of tempeh wrapping on sensory evaluation and lactic acid bacteria profile

Tempeh is a fermented food made from soybeans and consumed daily due to its delicious flavour and high nutritional value. Traditionally, tempeh is wrapped in banana leaf, but nowadays, tempeh wrappers are often replaced with plastic because it is cheap and practical. However, high plastic utilization in daily life results in waste accumulation and causes environmental issues. Therefore, different types of leaves were explored as wrapping alternatives to reduce plastic utilization. This study aimed to determine the sensory characteristics of tempeh made with various wrappers and the lactic acid bacteria (LAB) characteristics of each tempeh. In this study, tempeh was wrapped in a teak leaf, bamboo leaf, lotus leaf, coconut leaf and plastic. Enumeration of total viable bacteria and LAB were not significantly different for all variants of the tempeh, except for the lotus leaf variant, which had a significantly lower number of LAB. The teak and coconut leaf variant tempeh were acceptable to panellists in the sensory evaluation test. The most preferred variant was the plastic-wrapped variant which had no significant difference from commercial tempeh. All LAB from the five types of tempeh were isolated to evaluate their haemolytic activity and antibiotic resistance. The result showed that all isolates exhibited negative haemolytic activity (gamma haemolysis). Most isolates (90.8%) were susceptible to chloramphenicol, while 80.3% of the isolates were resistant to vancomycin. The five isolates with the best characteristics were identified using the 16S rRNA gene. They were identified as Enterococcus faecium, Staphylococcus gallinarum and Staphylococcus xylosus.

P1' specificity of the S219V/R203G mutant tobacco etch virus protease.

Proteases that recognize linear amino acid sequences with high specificity became indispensable tools of recombinant protein technology for the removal of various fusion tags. Due to its stringent sequence specificity, the catalytic domain of the nuclear inclusion cysteine protease of tobacco etch virus (TEV PR) is also a widely applied reagent for enzymatic removal of fusion tags. For this reason, efforts have been made to improve its stability and modify its specificity. For example, P1' autoproteolytic cleavage-resistant mutant (S219V) TEV PR was found not only to be nearly impervious to self-inactivation, but also exhibited greater stability and catalytic efficiency than the wild-type enzyme. An R203G substitution has been reported to further relax the P1' specificity of the enzyme, however, these results were obtained from crude intracellular assays. Until now, there has been no rigorous comparison of the P1' specificity of the S219V and S219V/R203G mutants in vitro, under carefully controlled conditions. Here, we compare the P1' amino acid preferences of these single and double TEV PR mutants. The in vitro analysis was performed by using recombinant protein substrates representing 20 P1' variants of the consensus TENLYFQ*SGT cleavage site, and synthetic oligopeptide substrates were also applied to study a limited set of the most preferred variants. In addition, the enzyme-substrate interactions were analyzed in silico. The results indicate highly similar P1' preferences for both enzymes, many side-chains can be accommodated by the S1' binding sites, but the kinetic assays revealed lower catalytic efficiency for the S219V/R203G than for the S219V mutant.

Features of Staphylococcus Aureus Antibiotic Sensitivity in Children with Atopic Dermatitis

Background. Excessive colonization of the skin by various bacteria and fungi can be noted in patients with atopic dermatitis (AD), and the prevalence of secondary infection complications 30–48%. Several studies have shown that Staphylococcus aureus colonization is 60–100% in patients with AD compared to 5–30% in healthy persons from the control group. Moreover, the incidence of methicillinresistant Staphylococcus aureus (MRSA) isolates is up to 10–30% in skin cultures at AD, according to experts. Therapy of AD complicated by secondary infection is one of the crucial challenges of modern dermatology. Mupirocin can be considered as one of the most effective topical antibiotic among others used for etiotropic therapy of infectious complications in AD patients, and it has been confirmed by numerous clinical studies.Conclusion. Staphylococcus aureus is the most common trigger of AD aggravation. MRSA in AD complicated by secondary infection is difficult to manage as it becomes resistant to many types of antibiotics; however, it shows persistent sensitivity to mupirocin. Mupirocin-based (2%) external agent is the most effective, safe, and preferred therapy variant for AD complicated by secondary infection in pediatrics.

In situ synchrotron X-ray diffraction analysis of two-way shape memory effect in Nitinol

Despite the fact that the Two-Way Shape Memory Effect (TWSME) has been demonstrated in most Shape Memory Alloys, the effective application of this unique functional behaviour is hindered by the lack of a proper training methodology and understanding of its mechanisms. In this study, a novel training routine has been established together with a home-designed device, enabling TWSME of customised spline curvature to be produced. An in situ high energy synchrotron X-ray diffraction experiment has been performed on Nitinol, followed by comprehensive analysis to reveal the micromechanics of TWSME. Multiple mainstream hypotheses have been examined. The important findings are: (1) The training process has negligible influence on the texture of parent phase; (2) The preferred variant of the B19’ phase exhibits tension/compression asymmetry in TWSME; (3) (100) compound twin is the preferred deformation mode for compression TWSME; (4) The mesoscale residual strain field is the dominant factor that induces TWSME; (5) Lattice defects (dislocations) are spatially rearranged after training; (6) Compression TWSME training retards the B2 to B19’ transformation, whilst tension has the opposite effect. The implications of these findings are further discussed.

A Novel Hybrid Recommender System for the Tourism Domain

In this paper, we develop a novel hybrid recommender system for the tourism domain, which combines (a) a Bayesian preferences elicitation component which operates by asking the user to rate generic images (corresponding to generic types of POIs) in order to build a user model and (b) a novel content-based (CB) recommendations component. The second component can in fact itself be considered a hybrid among two different CB algorithms, each exploiting one of two semantic similarity measures: a hierarchy-based and a non-hierarchy based one. The latter is the recently introduced Weighted Extended Jaccard Similarity (WEJS). We note that WEJS is employed for the first time within a recommender algorithm. We incorporate our algorithm within a real, already available at Google Play, tour-planning mobile application for short-term visitors of the popular touristic destination of Agios Nikolaos, Crete, Greece, and evaluate our approach via extensive simulations conducted on a real-world dataset constructed for the needs of the aforementioned mobile application. Our experiments verify that our algorithms result in effective personalized recommendations of touristic points of interest, while our final hybrid algorithm outperforms our exclusively content-based recommender algorithms in terms of recommendations accuracy. Specifically, when comparing the performance of several hybrid recommender system variants, we are able to come up with a “winner”: the most preferable variant of our hybrid recommender algorithm is one using a ⟨four elicitation slates, six shown images per slate⟩ pair as input to its Bayesian elicitation component. This variant combines increased precision performance with a lightweight preferences elicitation process.

The Ketogenic Diet but not Hydroxycitric Acid Keeps Brain Mitochondria Quality Control and mtDNA Integrity Under Focal Stroke.

Mitochondrial dysfunction in the ischemic brain is one of the hallmarks of stroke. Dietary interventions such as the ketogenic diet and hydroxycitric acid supplementation (a caloric restriction mimetic) may potentially protect neurons from mitochondrial damage induced by focal stroke in mice. We showed that in control mice, the ketogenic diet and the hydroxycitric acid did not impact significantly on the mtDNA integrity and expression of genes involved in the maintenance of mitochondrial quality control in the brain, liver, and kidney. The ketogenic diet changed the bacterial composition of the gut microbiome, which via the gut-brain axis may affect the increase in anxiety behavior and reduce mice mobility. The hydroxycitric acid causes mortality and suppresses mitochondrial biogenesis in the liver. Focal stroke modelling caused a significant decrease in the mtDNA copy number in both ipsilateral and contralateral brain cortex and increased the levels of mtDNA damage in the ipsilateral hemisphere. These alterations were accompanied by a decrease in the expression of some of the genes involved in maintaining mitochondrial quality control. The ketogenic diet consumption before stroke protects mtDNA in the ipsilateral cortex, probably via activation of the Nrf2 signaling. The hydroxycitric acid, on the contrary, increased stroke-induced injury. Thus, the ketogenic diet is the most preferred variant of dietetic intervention for stroke protection compared with the hydroxycitric acid supplementation. Our data confirm some reports about hydroxycitric acid toxicity, not only for the liver but also for the brain under stroke condition.

Анализ альтернативных вариантов однопролетных мобильных канатных дорог на базе самоходных шасси

Currently, mobile ropeways are considered as one of the promising types of rapidly deployable transport systems, which are formed with the help of two transport and reloading rope complexes based on autonomous self-propelled multi-purpose chassis combined by a single aerial carrying-traction rope system. The key problem in creating effective mobile ropeways of this type is the choice of their rational design, which allows to obtain the best tactical and technical characteristics and quality indicators as a result of the design. The article presents and analyzes possible alternative variants of single-span mobile ropeways based on mobile transport and reloading rope complexes. Based on the results of the comparative analysis, the most preferred variant was established — a single-span single rope mobile ropeway of the pendulum type with a carrying-traction rope system in the form of a closed carrying-traction rope with two parallel branches. The article considers the typical design of the most preferred variant and describes the principle of its operation, as well as lists the main components and mechanisms of the main technological equipment, which should be part of mobile transport and reloading rope complexes.

Про спільні зміни ВВП за паритетом купівельної спроможності на душу населення України та Азербайджанської Республіки

The article examines the relationships between the key components of the levels of socioeconomic development of Ukraine and the Republic of Azerbaijan. The aim of the study is to build a model of intercountry regression of per capita economic growth of both Ukraine and the Republic of Azerbaijan and to perform a comparative cross-country analysis of the key component of inclusive growth of the two countries under the strong influence of financial and socioeconomic crisis processes and the ongoing war. The study is aimed at an empirical study of the joint movement of both indicators, the identification of cause-and-effect relationships and cointegration dependence, the determination of a long-term integration relationship, allowing to take the necessary steps and measures for the positive mutual impact of trade and economic relations between the two countries on the national socioeconomic development. As the level of economic development, GDP per capita was used, taking into account purchasing power parity (PPP). The analysis of the dynamic relationship of indicators is carried out on the basis of data from January 1990 to December 2022. The study uses econometric methodology, including the extended Dickey–Fuller test for the presence of a single root, the Granger test for causality, the Engle–Johansen tests for cointegration, the vector model of error correction, and standard diagnostic tests. Tests for stationarity, causality, and cointegration were performed at the 10% significance level in the entire sample. The cointegration of the integrated 1st order of non-stationary initial series is revealed, where the preferred variant is the one with a trend, in which the marginal rate of restoration of equilibrium between GDP per capita at PPP of the two countries in the current year after the shock of the independent variable in the previous year is noted. The result, showing that GDP per capita at the PPP of Ukraine is in a slight positive dependence on GDP per capita at the PPP of Azerbaijan, is obtained. The empirical model explains 95% of the variation in the initial levels, and the equation of the vector error correction system 51% explains the differences. Recommendations are provided to ensure a long-term equilibrium of the joint movement of GDP at the PPP of both countries and to reduce the imbalance

Modified Sorghum Flour as a Wheat Substitute in Functional Food Muffin Products

Muffin products have become increasingly popular as a functional food due to its antioxidant activity. Post-Covid-19 consumers have shown particular interest in foods that can boost their immune systems, making muffins a desirable option. This study aims to explore the potential of modified sorghum flour as a substitute for wheat in muffin production to meet the increasing demand for functional foods with antioxidant activity. A completely randomized design was employed to test six different flour combinations in muffin preparation, ranging from 100% wheat flour to 100% modified sorghum flour. The study evaluated the chemical quality, antioxidant activity, and panelist acceptance level of each muffin variant. The results demonstrated that a combination of wheat flour and modified sorghum flour, with 60% modified sorghum flour and 40% wheat flour, produced the most preferred muffin variant among panelists with 5.04% antioxidant activity. Furthermore, the study utilized pre-gelatinization method in processing modified sorghum flour, which is a feasible technology for community-level applications. These findings suggest the potential of modified sorghum flour as a wheat substitute in functional muffin production.

On the Problem of Separate and Hyphenated Spelling of Russian Toponyms with Postpositive Adjective

The paper explores the problem of separate and hyphenated spelling of place names with a postnominal adjective such as Strugi(-)Krasnye. It provides a general review of the spelling rules for place names that include adjectives and covers various structural and semantic types of toponyms with postpositive adjectives. Using examples retrieved from dictionaries and legal documents (State Index of Place Names, etc.) the author identifies the preferred spelling variants for each type of toponym, as well as possible spelling motivations, investigates the orthographic history and officially approved spellings of toponyms including the settlement name Strugi Krasnye and the Strugo-Krasnensky district. It has been established that the codification of place names with a postpositive adjective is not fully aligned with the current rule on their hyphenated spelling, which poses the problem of choosing the right spelling policy to use in such cases. The two main factors that influence this choice are: the generally accepted separate spelling of structures with a postpositive inflected adjective, on the one hand, and the tendency to hyphenize the components of the name to create a compound, on the other. The positive and negative sides of both choices are considered. The conclusion is that the hyphenated spelling of the “noun + adjective” pattern is more convenient as it allows us to rely on formal features without looking into the history of the name formation: to apply this rule correctly it only takes to determine if the second component is an adjective by form (although by origin it can be, in fact, a standalone name, i.e. a substantivized adjective). At the same time, changing the rule will create a problem of orthographical differentiation between such names as Ilyinskoye-Khovanskoye and Ilyinskoye Kesemskoye.

Design of a container grabbing and lifting mechanism for equipping a robotic wheeled chassis

This article presents the results of designing a mechanism for grabbing, lifting and holding a container with which it is supposed to equip a robotic wheeled chassis with an apple harvesting module in an industrial garden. The technical solutions used in agricultural engineering for lifting and holding operations of various types of loads weighing from 300 kg were analyzed, their design and technical features were considered. As a result of the analysis, based on the criteria of compactness and metal consumption, the most preferred variant of the lifting mechanism was selected - a parallelogram lifting mechanism driven by electric cylinders. In order to fulfill the conditions of structural strength of the mechanism for gripping and lifting the container, a strength calculation of the structure was carried out, and also the necessary characteristics of the electric cylinder for the mechanism for lifting the container and the paws for the mechanism for lifting the paws were determined. The main load in the structure is carried by the upper horizontal guides, vertical racks and lower grips. As a result of the analysis, zones of maximum stress and strain concentration were established. The loading of the lower gripper parts occurs with a force directed vertically downward with a force of 1750 H. The highest voltage was about 169 MPa, which ensures sufficient strength of the part with a margin of 3 times, the maximum movements of the guides are 1.6 mm.

Development of model for forecasting and controlling the debt burden in a commercial organization

The COVID-19 pandemic has had a significant negative impact on the state of commercial organizations in the real sector. Therefore, the development of new solutions in forecasting and monitoring the functioning of organizations and their implementation in practice are vital to overcome the crisis. The main purpose of this article is to specify the marginal ranges of indicators of the debt burden of a particular commercial organization characterizing the correlation between the components of capital structure and indicators characterizing the level of security of liabilities with the assets of the organization on the basis of the results of prediction of the developed training model of the behavioral theory of capital by the method of matrices. One of the main advantages of the proposed approach is the determination of marginal ranges in line with the predicted preferred variants of liabilities for the sets of indicators assessing the debt burden. Within the scope of this article, this method has been carried out for an Armenian brandy company “Proshyan Brandy Factory” LLC. Using this model will, in the future, enable to determine common marginal ranges of indicators underlying the development of financial policy for a particular commercial organization in terms of the components of solvency, liquidity, business activity, profitability and creditworthiness.

Difficult-to-measure integration measurement method for designing processes in a chain-like structure of conflicted cells in a supply chain

The paper presents an approach for integrating conflicted cells in a chain-like structure based on a measurement of the level of integration of this structure. The authors’ prior experience suggests an assumption that conflicts always arise when cells are connected by a flow of materials, information, and money, although at different intensity levels. Usually, the intensity levels of said conflicts depend on the cooperation culture within a supply chain. This is a highly multidi-mensional problem, reflected by the literature referred to in this paper and related to this subject matter. The proposed approach treats technological processes between conflicted cells as an area of potential integration of these cells. Then, the integration becomes a problem of selecting the appropriate variant of realizing a technological process conducted in an area common for potentially conflicted cells of a company. The same approach may be used for cells belonging to separate production and distribution companies operating within a mutual supply chain. The paper proposes a method for measuring the integration level of neighboring cells, as a difficult-to-measure feature, by deter-mining an integration loss index. Also, the paper presents a multi-criteria method based on the fuzzy set theory for selecting a preferred variant of a technological process. A presented example uses this method in a supply chain to maximize the integration level between Shipping and Recipient cells/links. It has been assumed that the preferred realization variant of the flow process will be based on the following criteria: 1) shipping cost; 2) shipping quality; 3) shipping time. Also, an algorithm is shown for applying the presented methodology, which is helpful for managers interested in increasing the integration level of a supply chain. The methodology allows for increasing the integrity of conflicted cells both in newly designed supply chains as well as in existing ones when remodeled or reorganized. Moreover, the paper indicates some problems associated with the efficient implementation of the proposed method.

Přechod smluvních závazků při převodu závodu

This paper aims to research the mandatory transfer of contractual obligations under the institute of the transfer of enterprise (or its part) under the Czech law. The transfer of business enterprises was reintroduced in the Czech law in more than 30 years ago, however to this day, the case law has not been united regarding the transfer of contractual rights and obligations. The author tries to conduct research by solving two case studies relating to the statutory guarantee of the transferor for the transferred debts. The first case study relates to the question, whether the transferor guarantees fulfilment of debts under specified framework agreements, provided the creditor has not consented to the transfer of the enterprise. In case of first study, the author succeeded to find solution under the current case law of the Czech Supreme Court. The aim of the second case study is to answer the question of “shared agreements” in case of the transfer of part of business enterprise, in particular whether there are any principles of determination which rights and obligations under shared agreement are subject of the transfer. According to the Czech Civil Code, the same principles as in case of the transfer of a whole enterprise should also be applied to the transfer of part of an enterprise, however there is no statutory nor doctrinal support on how to proceed in case of shared agreements. Although the current doctrine and case law provide no specific principles regarding which rights and obligations are transferred along with the part enterprise, the author provides an approach based on logical and teleological interpretation of the principles of enterprise transfer. The most preferred variant is to split the respective contractual rights and obligations, and if not possible, the rights and obligations should follow the legal fate of the part of enterprise to which they relate more closely.

Contribution of variant subunits and associated factors to genome-wide distribution and dynamics of cohesin

BackgroundThe cohesin complex organizes the genome-forming dynamic chromatin loops that impact on all DNA-mediated processes. There are two different cohesin complexes in vertebrate somatic cells, carrying the STAG1 or STAG2 subunit, and two versions of the regulatory subunit PDS5, PDS5A and PDS5B. Mice deficient for any of the variant subunits are embryonic lethal, which indicates that they are not functionally redundant. However, their specific behavior at the molecular level is not fully understood.ResultsThe genome-wide distribution of cohesin provides important information with functional consequences. Here, we have characterized the distribution of cohesin subunits and regulators in mouse embryo fibroblasts (MEFs) either wild type or deficient for cohesin subunits and regulators by chromatin immunoprecipitation and deep sequencing. We identify non-CTCF cohesin-binding sites in addition to the commonly detected CTCF cohesin sites and show that cohesin-STAG2 is the preferred variant at these positions. Moreover, this complex has a more dynamic association with chromatin as judged by fluorescence recovery after photobleaching (FRAP), associates preferentially with WAPL and is more easily extracted from chromatin with salt than cohesin-STAG1. We observe that both PDS5A and PDS5B are exclusively located at cohesin-CTCF positions and that ablation of a single paralog has no noticeable consequences for cohesin distribution while double knocked out cells show decreased accumulation of cohesin at all its binding sites. With the exception of a fraction of cohesin positions in which we find binding of all regulators, including CTCF and WAPL, the presence of NIPBL and PDS5 is mutually exclusive, consistent with our immunoprecipitation analyses in mammalian cell extracts and previous results in yeast.ConclusionOur findings support the idea that non-CTCF cohesin-binding sites represent sites of cohesin loading or pausing and are preferentially occupied by the more dynamic cohesin-STAG2. PDS5 proteins redundantly contribute to arrest cohesin at CTCF sites, possibly by preventing binding of NIPBL, but are not essential for this arrest. These results add important insights towards understanding how cohesin regulates genome folding and the specific contributions of the different variants that coexist in the cell.

The Philosophy of Debt Burden Management in Commercial Organizations

The COVID-19 pandemic has had a negative impact on the financial system and the financial condition of commercial organizations in the real sector. Therefore, the development of new solutions in the financial management system and their implementation in practice is vital to overcome the crisis. The main purpose of this paper is to improve the behavioural theory of capital by developing a learning model. According to finance management philosophy, the forecast results were used to adjust the marginal ranges between a specific commercial organization’s debt burden, capital structure, and indicators of the organization’s liabilities secured by the organization’s assets. One of the main advantages of the proposed approach is the determination of marginal ranges in line with the predicted preferred variants of liabilities for the sets of indicators assessing the debt burden. Within the framework of this article, this method was implemented for the studied Armenian organizations. The proposed model, with an overview of the philosophy of financial management, makes it possible to determine the general marginal ranges of the indicators that are the basis of financial policy development.