Preferred Location Research Articles

Sedimentary exhalative Pb-Zn deposit model of the Ban Lin – Phia Dam ore range, Vietnam

Purpose. The aim of this study is to construct a typical ore model for Pb-Zn ore range in Ban Lin – Phia Dam and to compare it with the existing ones. The model is constructed based on three main elements of the mineral generation process, such as formation environment, generated-ore fluid source, and deposited-ore mechanism. The obtained results are aimed at determining and predicting the resource of sedimentary exhalative (SEDEX) Pb-Zn ore deposit in Vietnam. Methods. To comprehend the characteristics of the SEDEX Pb-Zn ore deposits in the Ban Lin – Phia Dam area, we employ a combination of approaches that include mineralography to determine the composition and tectonic structure of rocks and ore, inclusion analysis to determine the temperature and inclusion composition, Pb isotopic analysis and stable S isotopic analysis to explore the environment and sources of ore-forming materials. Findings. The sedimentary exhalative (SEDEX) Pb-Zn ore deposit arises from the dissolution of metal-rich salt deposits due to heating at shallow depths (several kilometers). Subsequently, this fluid migrates through faults and fractures to preferred locations where galena and sphalerite precipitate alongside sedimentary basin deposition. Originality. SEDEX ore deposits have been extensively studied in various regions of the world. Although there are multiple theories regarding their origin, including explosion, biological debris accumulation, and surface replacement, each model has its own distinct advantages and limitations in explaining the genesis and development of SEDEX ore deposits. Practical implications. The acquired findings are intended to identify and predict the resources of sedimentary exhalative Pb-Zn ore deposits in Vietnam.

Diverse Engraftment Capability of Neuroblastoma Cell Lines in Zebrafish Larvae.

Xenotransplantation of neuroblastoma cells into larval zebrafish allows the characterization of their invivo tumorigenic abilities and high-throughput treatment screening. This established preclinical model traditionally relies on microinjection into the yolk or perivitelline space, leaving the engraftment ability of cells at the hindbrain ventricle (HBV) and pericardial space (PCS), sites valuable for evaluating metastasis, angiogenesis, and the brain microenvironment, unknown. To address this gap in knowledge, Casper zebrafish at 48 h postfertilization were microinjected with approximately 200 Kelly, Be(2)-C, SK-N-AS, or SY5Y cells into either the HBV or PCS. Fish were imaged at 1, 3, and 6 days postinjection and tumor growth was monitored at each timepoint. We hypothesized that engraftment ability and location preference would be cell line dependent. Kelly and SK-N-AS cells were able to engraft at both the HBV and PCS, with a near doubling in size of tumor volume during the 6 days observation period, with cells appearing to grow better in the HBV. Be(2)-C tumors remained static while SY5Y tumors decreased in size, with almost complete loss of volume at both sites. Therefore, the capability of neuroblastoma cell engraftment in zebrafish larvae is cell line dependent with a location preference.

Analyses of sea turtle landing behavior based on frequently observed coastal profile data - A case study in Enshu Coast, Japan

This study analyses sea turtle landing behavior along a 10 km sandy beach on the Enshu Coast, Japan, facing the Pacific Ocean. Data for the analysis are sea turtles' landing positions and crawl tracks recorded by a handheld GNSS device from May to September (2011–2016), noting spawning or no-spawning activity simultaneously, supplemented by weekly cross-shore beach profiles surveyed at four locations, satellite images, and other geographical data. At the onset, landing positions on the beach are visualized to determine local concentrations along the beach. Subsequently, we estimate the distance of spawning and no-spawning positions from the waterline during landing and evaluate beach stability through the temporal elevation change standard deviation. The study found that preferred spawning locations are 40–70 m from the waterline, above the intertidal zone, on bare sand, with a standard deviation of 0.1–0.6 m. This study also highlights the impact of beach infrastructure on sea turtle spawning failures and the influence of significant wave height on sea turtle landing.

Abstract A125: A nationwide initiative to expand access to lung cancer screening: Results and impact of the Color/American Cancer Society Lung Screening Access Program

Abstract Lung cancer is a leading health challenge in the United States – it is the third most common cancer and disproportionately impacts people of color. Early detection of lung cancer is possible through routine screenings using low-dose lung CT scans. Despite the efficacy of screening, 80-85% of people eligible for screening are not up-to-date. The underutilization of regular screening has often been attributed to gaps in primary care and insufficient support following an abnormal result. In an effort to improve access, Color and the American Cancer Society (ACS), launched a nationwide initiative to make lung cancer screening readily accessible and free across all 50 states based on ACS guidelines. The program simplifies the screening process by managing all logistical steps from physician orders to finding and booking appointments at local clinics to assisting with next steps if abnormalities are detected.Since the program launched in November 2023, 74 people completed the online health risk assessment. On average participants were 54 years of age (range: 18-81 years), 64% (n=41) female, and 65% (n=48) White. 28% (n=19) respondents currently smoked, 50% (n=34) had a former history of smoking, and average smoking history was 23.0 pack years (range: 0.1 - 66 pack years). While the program primarily supports individuals who meet ACS lung screening guidelines, all participants can speak with a clinician about screening.Of those who completed the health risk assessment, 45.6% (n=31) met ACS eligibility criteria for lung screening. Eligible participants were on average 62 years of age (range: 51 - 77 years), 74% (n=23) were female, and 87% (n=27) were White. Eligible participants resided in 21 states, with Georgia and Ohio having the highest number of eligible individuals.To date, 9 people have completed a lung CT and 3 are in progress of scheduling. On average, appointments were scheduled within 25 days from the initial referral submission (range: 8-55 days) and all appointments were within 17 miles from the individual’s preferred location (average: 6.8 miles, range: 0.5-17 miles).3 participants had a change in their lung screening plan based on their result, with 2 participants having Lung-RADS 3 category nodules (follow-up in 6 months) and 1 having Lung-RADS 4A nodules (follow-up in 6 months). Other findings included an ascending aortic aneurysm (4.2 cm), a hiatal hernia, an indeterminate adrenal nodule, and mild coronary artery calcification, some of which required additional follow-up care with a PCP or cardiologist within 3-12 months. No participants were recommended to have immediate diagnostic testing for lung cancer based on their results. The Color/ACS Lung Screening Access Program demonstrates the impact that a virtual lung cancer screening program has on closing screening gaps. It underscores the critical role of supporting patients not only in undergoing cancer screening, but also in navigating the complexities of follow-up care, thereby enhancing the overall efficacy of lung cancer prevention and early detection strategies. Citation Format: Deanna Brockman, Jennifer Fung, Ashly Emig, Gabrielle Hands, Wendy McKennon, Jennifer Cho, Robert Smith, William Dahut, Alicia Zhou, Keegan Duchicela. A nationwide initiative to expand access to lung cancer screening: Results and impact of the Color/American Cancer Society Lung Screening Access Program [abstract]. In: Proceedings of the 17th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2024 Sep 21-24; Los Angeles, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2024;33(9 Suppl):Abstract nr A125.

Care seeking during pregnancy: testing the assumptions behind service delivery redesign for maternal and newborn health in rural Kenya.

A health systems reform known as Service Delivery Redesign (SDR) for maternal and newborn health seeks to make high-quality delivery care universal in Kakamega County, in western Kenya, by strengthening hospital-level care and making hospital deliveries the default option for pregnant women. Using a large prospective survey of new mothers in Kakamega County, we examine several key assumptions that underpin the SDR policy's theory of change. We analyse data on place of delivery, travel time and distance, out-of-pocket spending, and self-reported quality of care for 19 127 women prospectively enrolled during antenatal care (ANC) and surveyed two times after their delivery. We analyze changes in womens' delivery location preferences in recent years in Kakamega, and over the course of their most recent pregnancy. We also evaluate travel time, out-of-pocket expenditures and patient satisfaction for women who deliver in public hospitals vs primary health centres. We find substantial changes in delivery location at the population level over time and for individual women over the course of pregnancy. Facility delivery has increased from 50.4% in 2010 to 89.5% in 2019; 70% of respondents deliver at a different facility than their reported intention at ANC. Out-of-pocket delivery expenditures are on average 1351 Kenyan shillings (Ksh) in hospitals compared to 964 Ksh in PHC (primary health care)s (P < 0.01). Transport expenditures are 337 Ksh for PHC level deliveries vs 422 Ksh for hospitals (P < 0.01). Self-reported average travel time is 51 min (PHC delivery) vs 47 min (hospital delivery) (P = 0.78). The average distance to a delivery location is 15.1 km for PHC deliveries vs 15.2 km for hospitals (P = 0.99). There were no differences in overall patient-reported quality scores, while some subcomponents of quality favoured hospitals. These findings support several key assumptions of the SDR theory of change in Kakamega County, while also highlighting important challenges that should be addressed to increase the likelihood of successful implementation.

Addressing workforce distribution and improving care: insights from trainees

Introduction: Plastic surgeons are maldistributed in New Zealand. Regional centres have fewer surgeons and lower surgeon to population ratios than larger metropolitan areas. There is no published data on the future work intentions of plastic surgical trainees in New Zealand. This study examines the career preferences and intentions of plastic surgery trainees in New Zealand as well as perceived barriers to working in a regional centre. Methods: An anonymous online survey was sent to 31 current plastic surgery trainees and recent graduates. Data collected included demographic information, rural background, previous experience in regional centres, family responsibilities, preferred work locations, aspirations for private practice and work–life balance preferences. Results: A total of 25 trainees completed the questionnaire, with a response rate of 81 per cent. A significant proportion of trainees have prior experience in smaller regional centres. Family-friendly work environments and employment for partners were identified as important considerations. The top-ranked regional centres to work in included Queenstown/Wānaka, Hawkes Bay, Nelson and Whangārei. Factors such as the presence of other plastic surgeons, leave policies, after-hours call arrangements and access to a private operating facility were considered essential. Financial considerations, access to quality schooling or childcare, employment opportunities for partners, opportunities for teaching and unit expansion were also important factors. Conclusion: The results of this survey provide insight into the personal and professional factors influencing the career decisions of plastic surgery trainees in New Zealand. The findings highlight the need for strategic workforce planning and engaging relevant stakeholders to address the maldistribution of plastic surgeons, ultimately improving access to specialised care and reducing healthcare disparities for New Zealanders.

Choosing where to work: an empirical study of collaborative activities’ impact on workspace choice behavior

Purpose In today’s rapidly evolving work landscape, the design of office spaces is a crucial concern for organizations. Companies are redefining offices as collaboration hubs to entice employees back to in-person work. However, the understanding of how employees choose their workspaces, especially for collaborative activities, and how this should inform office design is lacking. Workers’ collaborative activity patterns can help better understand workspace choice behavior (WCB). In two studies, this paper aims to explore which characteristics of collaborative activities to consider when reshaping offices. Design/methodology/approach Data collected in a cross-sectional study design at a research institution (n = 285) and a university (n = 352) were used for confirmatory factor analyses and regression analysis. Findings The first study shows that collaborative activities can be classified into three distinct types: coordinative activities (planned and formal), deep collaboration (planned and complex) and spontaneous communication (informal and short encounters). The second study revalidates this classification and reveals patterns impacting WCB. Frequency and location preference of spontaneous communication and work environment satisfaction are strong predictors of on-site work. Personal characteristics like gender, age, managerial position or commute time are less consequential than assumed. Practical implications The results pinpoint guidelines for office designers and leaders in shaping effective workspaces and policies. Originality/value This paper provides new insights into classifying collaborative activities and personal characteristics, activity characteristics and environmental factors influencing WCB.

Engineering bacteriophages through deep mining of metagenomic motifs.

Bacteriophages can adapt to new hosts by altering sequence motifs through recombination or convergent evolution. Where such motifs exist and what fitness advantage they confer remains largely unknown. We report a new method, Metagenomic Sequence Informed Functional Scoring (Meta-SIFT), to discover sequence motifs in metagenomic datasets that can be used to engineer phage activity. Meta-SIFT uses experimental deep mutational scanning data to create sequence profiles to enable deep mining of metagenomes for functional motifs which are otherwise invisible to searches. We experimentally tested over 17,000 Meta-SIFT derived sequence motifs in the receptor-binding protein of the T7 phage. The screen revealed thousands of T7 variants with novel host specificity with functional motifs sourced from distant families. Position, substitution and location preferences dictated specificity across a panel of 20 hosts and conditions. To demonstrate therapeutic utility, we engineered active T7 variants against foodborne pathogen E. coli O121. Meta-SIFT is a powerful tool to unlock the functional potential encoded in phage metagenomes to engineer bacteriophages.

Comparison on Hysteresis Loops and Dislocation Configurations in Fatigued Face-Centered Cubic Single Crystals

The aggregation and evolution of dislocations form different configurations, which are the preferred locations for fatigue crack initiation. To analyze the spatial distribution of the same dislocation configuration and the resulting configuration morphologies on different observation planes, several typical hysteresis loops and dislocation configurations in fatigued face-centered cubic single crystals with various orientations were compared. The crystal orientations of these specimens were determined by the electron back-scattering diffraction technique in a Cambridge S360 Scanning Electron Microscope. It is well known that dislocation ladder and wall structures, as well as patch and vein structures, are distributed on their respective observation planes, (12¯1) and (111). These correspond to the point defect direction and line defect direction of dislocations, respectively. Therefore, the wall structures on the (12¯1) and (111) planes consist of point defects and line defects, which can be defined as point walls and line walls, respectively. Furthermore, the walls on the (12¯1) plane consist of Persistent Slip Band ladders connected with each other, corresponding to the formation of deformation bands. The evolution of dislocation patterns follows a process from patch to ladder and from vein to wall. The formation of labyrinths and dislocation cells originates from the activation of different secondary slip systems. In one word, it can help us better understand the physical nature of metal fatigue and failure by studying the distribution and evolution of different configurations.

Optimal location to land banking practices in urban-rural informal land market continuum of Ghana

Land banking practices in complex informal land markets are growing in developing countries. However, the land banking (LB) literature predominantly focuses on publicly driven land banks operating within formal land markets. Against this backdrop, this study investigates optimal locations for LB projects in Ghana's complex informal land markets from the perspective of private and semi-public real estate developers. Utilising a two-stage research process, first, the study developed a conceptual framework by using: (1) suppositions regarding space under economic geography; and (2) theoretical suppositions on the use of LB and its influence on LB locational choices uncovered from an interpretive hermeneutic literature review. The second stage focused on an empirical assessment of the conceptual framework by taking four urbanised regions in Ghana. The case study stage uses primary data from 30 interviewees selected using purposive and snowball sampling, while secondary data comprised land bank inventories from the regional Lands Commissions of the case study regions. Results revealed land title security as the primary factor determining optimal locations for land banks. There are significant challenges related to land title security in urban and inner parts of peri-urban areas. These challenges are aggravating the transformation of agricultural lands into residential lands in developers' preferred land bank locations. Based on the ongoing land transformation occurrences, the study underscores the need for policy responses that enhance title security to encourage developers to diversify their land banking locational preferences beyond solely greenfield sites to a mix of green and urban brownfield sites.

Harnessing Natural Language Processing to Assess Quality of End-of-Life Care for Children With Cancer.

Data on end-of-life care (EOLC) quality, assessed through evidence-based quality measures (QMs), are difficult to obtain. Natural language processing (NLP) enables efficient quality measurement and is not yet used for children with serious illness. We sought to validate a pediatric-specific EOLC-QM keyword library and evaluate EOLC-QM attainment among childhood cancer decedents. In a single-center cohort of children with cancer who died between 2014 and 2022, we piloted a rule-based NLP approach to examine the content of clinical notes in the last 6 months of life. We identified documented discussions of five EOLC-QMs: goals of care, limitations to life-sustaining treatments (LLST), hospice, palliative care consultation, and preferred location of death. We assessed performance of NLP methods, compared with gold standard manual chart review. We then used NLP to characterize proportions of decedents with documented EOLC-QM discussions and timing of first documentation relative to death. Among 101 decedents, nearly half were minorities (Hispanic/Latinx [24%], non-Hispanic Black/African American [20%]), female (48%), or diagnosed with solid tumors (43%). Through iterative refinement, our keyword library achieved robust performance statistics (for all EOLC-QMs, F1 score = 1.0). Most decedents had documented discussions regarding goals of care (83%), LLST (83%), and hospice (74%). Fewer decedents had documented discussions regarding palliative care consultation (49%) or preferred location of death (36%). For all five EOLC-QMs, first documentation occurred, on average, >30 days before death. A high proportion of decedents attained specified EOLC-QMs more than 30 days before death. Our findings indicate that NLP is a feasible approach to measuring quality of care for children with cancer at the end of life and is ripe for multi-center research and quality improvement.

Typical morphological characteristics of the immunohistochemical subtypes of pituitary microadenomas: a dual center study.

This study aimed to investigate the relationship between magnetic resonance image (MRI) features and the immunohistochemical subtypes of pituitary microadenomas (PMAs) characterized by location and growth pattern. A double-center, retrospective review of MRI characteristics was conducted in 57 PMA cases recorded from February 2014 to September 2023, identified based on the 2017 World Health Organization classification of pituitary gland tumors. The geometric center of the tumor was defined, and the possibility of PMA vertical or lateral growth patterns was evaluated according to the ratio of maximum diameter between the X and Y axes. Among the PMAs, somatotroph adenomas (STAs) significantly frequented the lateral-anteroinferior portion of the pituitary gland (P = 0.036). Lactotroph adenomas (LTAs) showed a significant locational preference for the lateral-posteroinferior portion (P = 0.037), and gonadotroph adenomas (GTAs) were predominantly located in the central-anteroinferior portion (P = 0.022). Furthermore, PMAs in the suprasellar portion exhibited vertical extension with statistical significance (P = 0.0). In our cohort, micro-STAs were predominantly located in the lateral-anteroinferior portion of the pituitary gland, micro-LTAs in the lateral-posteroinferior portion, and micro-GTAs in the central-anteroinferior portion. The growth pattern of PMAs was highly correlated with their vertical position instead of their immunohistochemical subtypes. Therefore, MRI shows potential in differentiating partial PMA subgroups, especially cases within the silent groups.

Consumers' familiarity level shapes motives and contexts for preparing and consuming dishes.

Consumers' familiarity with foods influences their expectations and experiences upon consumption. Familiarity has mostly been assessed using perceived familiarity when studying single foods. However, foods are often combined to create dishes, complex compositions eaten at various mealtimes. Hence, this paper aimed to study if and how the level of consumers' dish familiarity shapes their motives and preferred contexts for home cooking and consuming three dishes: one traditional dish, one adopted ethnic dish, and one mainly unavailable and unknown ethnic dish. First, we describe the operationalization of familiarity into the qualitative "consumer's dish familiarity index" (CDFI) used to determine participants' familiarity level to the dishes. CDFI includes four indicators (perceived availability, consumer's knowledge, preparation, and consumption). In-depth interviews (n=27) were conducted with Dutch and Chilean women, as the countries have contrasting levels of immigration and globalization, which are expected to shape their inhabitants' familiarity level with ethnic dishes. Most experienced consumers (high familiarity) mentioned more dishes and individual-related motives to prepare and consume a traditional or an adopted ethnic dish. Social- and individual-related motives were salient when a dish was unknown and/or not previously consumed. Consumers' familiarity level also guided their preferred social context and location when consuming the dishes. A dish barely available will likely be first consumed in a restaurant to try a new dish that is perceived as appetizing to eat. Results showed that convenience, wanting to eat comfort foods, and showing affection to others are important motivators to facilitate the adoption of dishes to be home cooked. PRACTICAL APPLICATION: This paper provides a novel approach to assess consumer familiarity to dishes. The qualitative consumer's dish familiarity index (CDFI) can be used in future investigations to assess and classify consumers into different dish familiarity levels by using four indicators. The outcomes of this study suggest consumers' familiarity level could serve as a reference for designing and/or improving (ethnic) foods and creating marketing campaigns to introduce them into (foreign) countries.

Knowledge and Attitudes of Pregnant Women towards Bankjo Lamp Technology: Case Study of Choosing a Birth Place

Background: A high-risk pregnancy jeopardizes both maternal and fetal lives. Despite recommendations for hospital births, six out of ten high-risk expectant mothers in Kawedanan opt for PMB or Community Health Center Maternity. To identify high-risk pregnancies, Kawedanan Community Health Center employs the innovative Bankjo lamp. This study investigates how pregnant women's attitudes and knowledge about Bankjo lamps influence their maternity location choice. Methods: This type of research uses quantitative descriptive research. This observational analytical research involved 57 expectant mothers due to maternity in January, February, or March. Using a cross-sectional design, the study treated knowledge and attitudes about Bankjo lamps as independent variables, with maternity location preference as the dependent variable. Data collection methods included knowledge and attitude questionnaires and monthly midwife reports. The data was analyzed using the Chi-Square test and descriptive statistics in the form of frequency distribution with presentation. Results: Findings indicate that pregnant women generally possess adequate knowledge about suitable maternity locations, with some showing insufficient understanding. A significant correlation (p = 0.013) exists between knowledge levels and preferred birthplace. Despite the preference for hospital births among nearly all expectant mothers, a majority hold negative attitudes, strongly correlated (p = 0.000) with their birthplace choices. Interestingly, while most pregnant women understand the Bankjo lamp innovation well, they harbor negative attitudes toward it. Nevertheless, almost all opt for hospital births, prioritizing medical safety despite reservations about new technologies. Conclusions: The study underscores the need for government support in advancing maternal health initiatives, including innovative technologies like Bankjo lamps. Enhancing awareness and promoting positive attitudes among expectant mothers can foster safer childbirth practices. By disseminating knowledge and addressing misconceptions, healthcare providers can better support high-risk pregnancies, ensuring better outcomes for both mothers and babies.

Corrosion-Resistant Ultrathin Cu Film Deposited on N-Doped Amorphous Carbon Film Substrate and Its Use for Crumpleable Circuit Board.

Copper (Cu) is widely used as an industrial electrode due to its high electrical conductivity, mechanical properties, and cost-effectiveness. However, Cu is susceptible to corrosion, which degrades device performance over time. Although various methods (alloying, physical passivation, surface treatment, etc.) are introduced to address the corrosion issue, they can cause decreased conductivity or vertical insulation. Here, using the nitrogen-doped amorphous carbon (a-C:N) thin film is proposed as a substrate on which Cu is directly deposited. This simple method significantly inhibits corrosion of ultrathin Cu (<20nm) films in humid conditions, enabling the fabrication of ultrathin electronic circuit boards without corrosion under ambient conditions. This study investigates the origin of corrosion resistance through comprehensive microscopic/spectroscopic characterizations and density-functional theory (DFT) calculations: i) diffusion of Cu atoms into the a-C:N driven by stable C-Cu-N bond formation, ii) diffusion of N atoms from the a-C:N to the Cu layer heading the top surface, which is the thermodynamically preferred location for N, and iii) the doped N atoms in Cu layer suppress the inclusion of O into the Cu lattice. By leveraging the ultrathinness and deformability of the circuit board, a transparent electrode and a crumpleable LED lighting device are demonstrated.

Rotation as promotor of F1Fo-ATP synthase membrane preference location

Rotation as promotor of F1Fo-ATP synthase membrane preference location

The role of blood donation on wheels in the blood collection chain operational logistics during the first wave of coronavirus disease 2019 pandemic

Abstract: BACKGROUND AND OBJECTIVES: The blood collection agencies (BCAs) employ two major logistical modes of conduct for outdoor blood collection. First, the collection agency walks to the donor’s preferred location for routine blood donation drives. Second, the drive is conducted using a heavy-duty vehicle having all the facilities for blood donation in it – Blood Donation on Wheels – hereinafter referred to as mobile blood drive (MBD). The study aimed to review the role of MBDs during the coronavirus disease 2019 (COVID-19) pandemic. MATERIALS AND METHODS: This was a retrospective analysis of data concerning the blood collection at the outdoor blood donation drives such as the number of drives, blood donations in voluntary (total outdoor), routine blood drive (RBD), and MBDs during the COVID-19 pandemic with respect to referent data. RESULTS: There was a trend in the increase in MBDs (43.3% in 2020 vs. 35.6% in 2019), with odds of 1.38 (confidence interval [CI]: 0.99–1.9), P = 0.05, during the pandemic. There was a statistically significant increase in contribution in terms of blood donations from MBDs (25.8% in 2020 vs. 19.5% in 2019), with odds of 1.43 (CI: 1.4–1.5), P &lt; 0.05, during the pandemic. There was a statistically significant reduction in the rates of vasovagal reactions in blood donors donating blood at voluntary blood drives, RBD, and MBDs during the pandemic, in comparison with the referent data. CONCLUSION: The blood donation on wheels compliments the public outreach efforts of the BCAs, both in terms of the organization of the outdoor blood donation drives and the blood donations obtained thereof.

Biocompatible Re-Containing Block Copolymers for Intracellular pH Mapping in the PLIM Mode.

Monitoring of intracellular pH is of great importance since deviation of this parameter from the "normal" magnitudes can be considered as an indicator of various pathologies. Thus, the development of new efficient and biocompatible sensors suitable for application in biological systems and capable of quantitative pH estimation remains an urgent chemical task. Herein, we report the synthesis of a series of phosphorescent rhenium [Re(NN)(CO)2(PR3)2]+ complexes based on the NN diimine ligands containing pH-responsive carboxylic groups and styrene-containing phosphine ligands. The complexes, which display the highest pH sensitivity, were copolymerized with polyvinylpyrrolidone using the RAFT protocol to impart water solubility and to protect the chromophores from interaction with molecular oxygen. The resulting copolymers show an emission lifetime response onto pH variations in the physiological range. Cellular experiments with Chinese hamster ovary cells (CHO-K1) reveal easy internalization of the probes in cell culture and an approximately uniform distribution in cells, with some preference for location in acidic compartments (late endosomes and lysosomes). Using nigericin to homogenize intra- and extracellular pH, we built a calibration of lifetime versus pH in live CHO-K1 cells. Analysis of the phosphorescence lifetime imaging microscopy (PLIM) data confirms the applicability of the obtained sensors for monitoring the intracellular pH in cell cultures.

Evaluation of Cocurricular Learning and the Influence of Student Choice

ObjectiveThe primary objective of this study was to evaluate the students’ choice of activities and perception of a cocurricular program (CCP). Attitude and skill development and areas for program improvement were also assessed. MethodsData were evaluated from 2 sources: a student survey administered to all Doctor of Pharmacy students to determine student perceptions of the CCP and barriers to success and student activity reporting data from postexperience submissions to evaluate the CCP. Data were stratified by student demographics to identify trends. A comparison of data was conducted from both sources on the students’ perception of learning value. ResultsData for 405 students were available for analysis. The highest preference overall from students for hosts of activities were professional student organizations; the highest preference of location was online, asynchronous. The most meaningful benefits were learning information about a new subject (n = 258, 63.7%), ability to extend learning from the classroom to real life (n = 247, 61%), and networking opportunities (n = 218, 53.8%). The top barriers for completion of the program included scheduling challenges (n = 296, 73.1%), lack of time (n = 249, 61.5%), and lack of interest (n = 187, 46.2%). Subpopulations identified different benefits and barriers to completion. ConclusionStudents have overall positive perceptions of the CCP and its value for skill development. Findings were applied to adjust the CCP at the institution, including a reduction in total hours required. The continuous evaluation of CCPs is important to optimize student learning and address curricular overload.

Location preference of boron and nitrogen dopants at graphene/copper interface

Controlling the placement of dopants can significantly tailor graphene's properties, but this process is influenced by copper substrates during vapor deposition. Understanding the influence of interfacial atomic structures on the preference for dopant locations is crucial. In this work, we conducted a systematic first-principles study of boron- and nitrogen-doped graphene on copper {111}, considering both sublattice and superlattice configurations. Our calculations revealed that the formation energy is minimized at the top-fccb site (−0.60 eV) for boron and the hcp-fcca site (1.94 eV) for nitrogen, suggesting a possible selective distribution of dopants in both sublattice and superlattice arrangements at the graphene/copper interface. Furthermore, a lower formation energy indicates a higher release of energy during doping, resulting in a stronger interfacial binding. Since formation energy is closely associated with out-of-plane interactions, while in-plane interactions remain relatively stable, these differences offer potential avenues for modifying dopant distribution at graphene/copper interfaces.