Preference For Palatable Food Research Articles

To B or not to B: Fat is the question

To B or not to B: Fat is the question

Effects of neonatal and peripubertal ethanol treatment on various aspects of adult rat behavior and brain anatomy

Exposure to ethanol during critical stages of brain development and maturation has adverse effects on behavioral and cognitive functions. So far, most animal models focused on the effects of either pre- or early postnatal ethanol treatment on behavior. We here used a multiple crossover design to investigate the effects of neonatal (postnatal day 7) ethanol treatment (2.5g/kg b.i.d., dissolved in saline), subchronic peripubertal (postnatal days 40–65) ethanol treatment (1.0g/kg, dissolved in saline) and the combination of both on the performance of adult Wistar rats in a variety of behavioral tasks. We also assessed anatomical changes in limbic and cortical brain areas.No effects of either single or combined neonatal and pubertal ethanol treatment was found on prepulse inhibition of startle (PPI, a measure of sensorimotor gating), or on the acoustic startle response in the absence of prepulses. Peripubertal ethanol treatment reduced the explorative behavior in the open field. The breakpoint in a progressive ratio operant response task was increased in those rats that had received both neonatal and pubertal ethanol treatment, while the preference for palatable food used as reinforcer in this task was not affected. No treatment effects were found on object recognition memory. No treatment effects on anxiety-related behavior in the elevated plus maze were found, however, the anxiolytic effect of the prototypical benzodiazepine diazepam was enhanced in rats that had received peripubertal ethanol treatment.Additive effects of neonatal and pubertal ethanol treatments were found on behaviors related to spontaneous locomotor activity. Combined neonatal and pubertal ethanol treatment lead to a reduction of myelin sheaths in the prefrontal cortex, and the neonatal ethanol treatment lead to a reduced number of parvalbumine-immunoreactive cells in the dorsal hippocampus.These findings suggest that neonatal ethanol exposure increases the risk of some but not all adverse behavioral and brain anatomical effects of pubertal ethanol consumption.

Could Preference for Palatable Foods in Neonatally Handled Rats Alter Metabolic Patterns in Adult Life?

Previous studies indicate that, in adulthood, neonatally handled rats consume more sweet food than nonhandled rats. The aim of the present study was to assess the effects of the chronic exposure to a palatable diet (chocolate) in adult neonatally handled rats. We measured the consumption of foods (standard lab chow and chocolate), body weight gain, abdominal fat deposition, and levels of plasma lipids, glucose, insulin, and corticosterone in adult neonatally handled (10 min/d, first 10 d of life) and nonhandled rats. We found an increased intake of chocolate in handled rats, but this consumption decreased over time. Handled male animals exhibited higher body weight, higher caloric efficiency, and lower triglyceride levels. Nonhandled females that were exposed long-term to the highly caloric diet had increased abdominal fat deposition compared with handled females. Overall female rats had increased abdominal fat deposition, higher total cholesterol and glucose levels, and lower insulin in comparison with males. Interestingly, chocolate consumption diminished the weight of the adrenal glands in both handled and nonhandled animals. These findings suggest that neonatal handling induces a particular metabolic pattern that is sex specific.

Preference for palatable food is reduced by the gamma-hydroxybutyrate analogue GET73, in rats

Palatability and variety of foods are major reasons for “hedonic” eating, and hence for overeating and obesity. Palatable food and drugs of abuse share a common reward mechanism, and compounds that block the reinforcing effect of drugs of abuse preferentially suppress the intake of palatable foods. This research was aimed at studying the influence of the gamma-hydroxybutyrate analogue N-(4-trifluoromethylbenzyl)-4-methoxybutanamide (GET73) – that inhibits alcohol consumption – on consumption and reinforcing effect of palatable food. Adult male rats were used. For place preference conditioning, sweetened corn flakes were used as the reinforcer, and GET73 (50, 100 and 200 mg kg −1) or vehicle were orally (p.o.) administered either 30 min before each training session and the test session, or only before the test session. To study the influence on consumption, GET73 was given p.o. at the same doses once daily for 12 days to rats given free access to both palatable and varied food (cafeteria diet) or to standard chow. Both acquisition and expression of palatable food-induced conditioned place preference were inhibited by GET73, either administered throughout the conditioning period or only before the test session. GET73 reduced also the consumption of cafeteria food, while that of standard chow was increased. At these doses, GET73 had no detrimental effect on open-field behaviour. GET73 seems to specifically attenuate the gratification produced by varied and palatable food, without affecting the consumption of not particularly palatable chow. Since, overweight and obesity are mostly due to the overeating of palatable and varied foods, drugs like GET73 could represent a somewhat ideal and rational approach to obesity treatment.

Binge Eating in Rats Produced by Combining Dieting with Stress

This unit describes a rodent model of binge eating based on cyclic restriction, refeeding, footshock, and intermittent access to palatable food. These conditions mimic dieting, stress, and "junk" food indulgence, respectively, all common etiological and maintenance factors in human binge eating. Four groups of rats are used: one subjected to cyclic food restriction, another to acute footshock stress, another to both of these (R + S), and a control. Neither cyclic restriction nor stress alone produces binge eating, but the R + S rats, despite satiety, double their intake of palatable food in a discrete period of time (i.e., binge) when stressed. This protocol recapitulates critical properties of human binge eating, namely preference for palatable food, dieting- and stress-induced vulnerability to binging, and eating for reward versus metabolic need. This protocol permits study of the psychobiological underpinnings of binge eating and possibly also of addiction, impulsivity, and depression, which are co-morbid with binge eating.

Neonatal Handling, Sweet Food Ingestion and Ectonucleotidase Activities in Nucleus Accumbens at Different Ages

Neonatal handled rats ingest more sweet food than non-handled ones, but it was documented only after puberty. Here, we studied the purinergic system in the nucleus accumbens, a possible target for the alteration in the preference for palatable food. We measured the ATP, ADP and AMP hydrolysis mediated by ectonucleotidases in synaptosomes of the nucleus accumbens in periadolescent and adult rats from different neonatal environments: non-handled and handled (10 min/day, first 10 days of life). Before adolescence, we found a decreased ingestion of sweet food in the neonatally handled group, with no effect on ATP, ADP or AMP hydrolysis. In adults, we found a greater ingestion of sweet food in the neonatally handled group, with no effect on ATPase or ADPase activities, but a decreased AMP hydrolysis. The nucleus accumbens is a site of intensive interaction between the adenosinergic and dopaminergic systems. Therefore, adenosine may modulate accumbens' dopamine neurotransmission differently in neonatally handled rats.