Predominance Of Leukocytes Research Articles

Inflammatory cell predominance and patterns in chronic rhinosinusitis with and without nasal polyposis patients.

ObjectivesThere is interest in identifying chronic rhinosinusitis (CRS) endotypes that align pathophysiology with clinical observation and outcomes. CRS with polyps (CRSwNP) has classically been studied with reference to tissue eosinophilia, but the role of other cellular infiltrates remains uncharacterized. No particular tissue prognosticators have been described for CRS without nasal polyps (CRSsNP). Predominance of leukocytes seen in surgical tissue may be useful for differentiating CRS subtypes, severity of inflammation, and outcomes.MethodsStructured histopathology reports were examined for 277 patients undergoing endoscopic sinus surgery for CRSwNP (n = 115), CRSsNP (n = 141), and recurrent acute rhinosinusitis (RARS, n = 21). Inflammatory predominance was examined for associations with nasal polyposis, asthma, allergic rhinitis, aspirin exacerbated respiratory disease (AERD), immune deficiency, preoperative Lund‐Mackay score, and outcome (SNOT‐22 score change).ResultsIn order of frequency, the prevalence of predominant inflammatory patterns accounting for 93.5% of CRS patients were: lymphoplasmocytic (n = 111), lymphocytic (n = 74), eosinophilic (n = 50), and lymphoplasmocytic with eosinophilic (n = 24). Eosinophilic predominance was 97.4% specific for nasal polyps (95% confidence interval [CI], 93.4%‐99.3%), although sensitivity was 43.4% (95% CI, 33.8%‐53.4%). The absence of eosinophilic predominance was 100% sensitive for RARS (95% CI, 82.4%‐100%), however specificity was 30.8% (95% CI 25.1%‐37.1%). There were no significant differences in preoperative SNOT‐22 scores or change postoperatively.ConclusionsEosinophilic inflammatory predominance was predictive for nasal polyps and against RARS. Nevertheless, the majority of CRSwNP patients had a different inflammatory predominance, demonstrating heterogeneity in CRS, even among patients with nasal polyps. Symptomatic outcomes were not associated with inflammatory predominance through 12 months follow up.Level of Evidence4.

Protocolo diagnóstico y tratamiento de las afectaciones pericárdicas de etiología infecciosa

TypesInfectious pericarditis encompasses three different aetiologies: viral, purulent and tuberculous. DiagnosisDiagnosis of each of these three entities requires specific study which should be protocolised. Viral pericarditis requires integral testing of the pericardial fluid or tissue which includes histological, cytological, immunohistological and molecular study. In the case of purulent pericarditis, diagnosis is based on findings of pericardial fluid of purulent appearance with a predominance of leukocytes and microbiological analysis. Finally, tuberculous pericarditis requires the tubercle bacillus to be demonstrated in the pericardial fluid or tissue. TreatmentTreatment will be specific to the aetiology.

Análise histológica e morfométrica da área cruenta tratada com membrana de silicone: estudo em ratos

This study evaluated the effects of a silicone membrane on the treatment of the raw flesh area in wounds. The experimental study was carried out with 30 male Wistar rats divided into three groups: in the GG group, the raw area was treated with gauze, in the GH group with homograft, and in the GS group with a silicone membrane. Animals were sacrificed at fourteen days of postoperative. The studied attributes were body mass variation, histological study quantified by morphometric analysis evaluating the number of neovessels, fibroblasts, collagen fibers, leucocytes, monocytes; and using a micrometric ruler measurement of the raw area's thickness. Data were then submitted to statistical analysis. There was no significant difference between the animals' mass (p=0.0685). Predominance of neovessels (p<0.01), fibroblasts (p<0.001) and thickness of the raw flesh were observed in G S animals. There was predominance of leucocytes (p<0.021) and monocytes (p<0.0001) in G H animals. Also, no significant difference between the groups as for collagen fibers (p=0.0536) was observed. The silicone membrane promoted granulation tissue with a large number of neovessels, fibroblasts and greater thickness.

Vaginal immunoglobulin A (IgA) levels in postmenopausal women: influence of oestriol therapy

The concentration of secretory immunoglobulin A (IgA) in the vaginal fluid of 20 untreated postmenopausal women (aged 75.4 ± 1.4 years) was compared with the levels in a group of 20 postmenopausal women (aged 73.2 ± 1.5 years) treated with oral oestriol (E 3) (2 mg/day) and a further group of 20 healthy, non-pregnant, fertile women (aged 28.2 ± 1.8 years). Secretory IgA was determined using a paper disc modification of the single radial immunodiffusion technique. The vaginal concentration of IgA in the untreated women was 41.5 ± 5.7 mg/l, which was higher than that recorded in those treated with E 3 (20.8 ± 5.7 mg/l, P < 0.05) and in the fertile women (16.4 ± 3.7 mg/I, P < 0.01). Epithelial cells predominated in the wet smear preparations obtained from the women who received E 3 and the fertile women, while a predominance of leucocytes was observed in the untreated women. Lactobacilli were found more frequently in the vaginal flora of the women in the E 3 group ( P < 0.001) and the fertile women ( P < 0.001) than the untreated group. Faecal-type bacteria were seen more frequently in the vaginal flora of the untreated women than the E 3 group ( P < 0.01) or the fertile women ( P < 0.001). The possible implications of the present findings with regard to the individual variation in the intensity of symptoms experienced by women suffering from the oestrogen deficiency syndrome are discussed.