The prediction of vibration characteristics was studied in the mistuned bladed disk by the mobile interface prestressed component mode synthesis (CMS) superelement method. When the strongly, generally, and weakly coupling in the mistuned bladed disk, according to the results of the direct FEM method, the prediction accuracy of this method was verified and compared with the fixed-interface CMS method by using the relative error of dynamic frequency, vibration mode matching function, and dimensionless root mean square error of vibration amplitudes. It is pointed that for mistuned bladed disk in the strong coupling, the prediction accuracy of dynamic frequency and vibration amplitudes are higher by the mobile interface CMS method and the vibration modes are matched with the direct method. In weak coupling, the results of dynamic frequency and vibration modes predicted by the mobile interface CMS method and the fixed-interface CMS method are consistent with the direct method, but the vibration amplitudes’ prediction error of the mobile interface CMS method is lower than that of the fixed-interface CMS method. In general coupling, the mobile interface CMS method has higher dynamic frequency prediction accuracy at low order, and the two methods have comparable dynamic frequency prediction accuracy at high order. The vibration modes predicted by the two methods are matched with the direct FEM method, and the prediction accuracy of vibration amplitude by the mobile interface CMS method is better than that of the fixed-interface CMS method. The results indicate that the mobile interface CMS method could more accurately predict vibration characteristics of the mistuned bladed disk with different coupling degrees and could be an effective measurement for studying the vibration characteristics of the mistuned bladed disk system.