Preconcentration Method Research Articles

Determination of copper at trace levels in fennel tea samples by flame atomic absorption spectrometry after the implementation of simultaneous complexation and supramolecular solvent based spraying assisted liquid phase microextraction

A simple and efficient preconcentration method named as supramolecular solvent based spraying assisted liquid phase microextraction (SUPRAS-SA-LPME) was combined with a flame atomic absorption spectrometry (FAAS) for the determination of copper in fennel tea samples at trace levels. An alkanol (1-decanol)/THF based SUPRAS (containing complexation agent) was used as extraction solvent instead of traditional organic solvents for the first time in an SA-LPME process. Under the optimum conditions, system analytical performance of the developed method was evaluated, and limit of detection (LOD)/limit of quantitation (LOQ) values were recorded as 1.91/6.37 µg kg–1. After the evaluation of system analytical performances, the enhancement in detection power/calibration sensitivity was found to be 21.3/21.8 folds when the comparison of LOD values/calibration plot slopes of the FAAS and SUPRAS-SA-LPME-FAAS systems. The method applicability was tested for the fennel tea samples by spiking experiments, and acceptable recovery results (80.2 % – 111.8 %) were obtained for low, mid and high spiked concentration levels.

A Novel Technology for the Recovery and Separation of Cassiterite- and Iron-Containing Minerals from Tin-Containing Tailing

Tin-containing tailing is classified as a solid waste, but it possesses valuable resources such as tin and iron. Tin-containing tailing exhibits a fine distribution and compact symbiosis of cassiterite- and iron-containing minerals. Therefore, it is difficult to recover and separate cassiterite- and iron-containing minerals using traditional mineral processing methods. The study proposed a novel technology involving pre-concentration, reduction roasting, and magnetic separation for the treatment of tin-containing tailings with a tin grade of 0.14% and an iron grade of 12.79%. The classification pre-concentration method was achieved using a combination of shaking tables, suspension vibration cone separators, and high-gradient magnetic separation with a magnetic field strength of 1.4 T. The discarded tailings ratio reached 73.56%. The gravity pre-enriched concentrates and magnetic pre-enriched concentrates underwent reduction roasting to facilitate the conversion of hematite and goethite into magnetite, respectively. The optimal conditions for reduction roasting of the gravity pre-enriched concentrate were a 10% lignite dosage, a roasting temperature of 650 °C, and a holding time of 80 min. The optimal conditions for reduction roasting of the magnetic pre-enriched concentrate were a 8% lignite dosage, a roasting temperature of 750 °C, and a holding time of 100 min. The reduction roasted products were treated using magnetic separation with a magnetic field strength of 0.16 T. Finally, a tin-rich middling with a tin grade of 2.93% and a recovery ratio of 70.88%, as well as an iron concentrate with an iron grade of 61.95% and a recovery ratio of 68.08% were obtained. The study achieved efficient recoveries of tin and iron from tin tailings, thereby presenting a novel approach for the utilization of resources in the tailing.

Paper-based isotachophoretic preconcentration technique for low-cost determination of glyphosate

Electrophoretic microfluidic paper-based analytical devices (e-µPADs) are promising for low-cost and portable technologies, but quantitative detection remains challenging. In this study, we develop a paper-based isotachophoretic preconcentration and separation method for the herbicide glyphosate as a model analyte. The device, consisting of two electrode chambers filled with leading and terminating electrolytes and a nitrocellulose strip as the separation carrier, was illuminated by a flat light source and operated with a voltage supply of 400 V. Detection was accomplished using a simple camera. Colorimetric detection was optimized through competitive complexation between glyphosate, copper ions, and pyrocatechol violet as a dye. The buffer system was optimized using simulations, (i) ensuring the pH was optimal for the demetallation of the blue pyrocatechol violet-copper complex [PV] to the yellow free dye and (ii) ensuring the electrophoretic migration of glyphosate into the slower [PV] for the colorimetric reaction. A new data evaluation method is presented, analyzing the RGB channel intensities. The linear range was between 0.8 and 25 µM, with a LOD of approximately 0.8 µM. The ITP separation preconcentrated glyphosate by a factor of 820 in numerical simulations. The method may be applied to control glyphosate formulations, especially in developing countries where herbicide sales and applications are poorly regulated.Graphical

Sustainable Recovery of Polyphenols and Carotenoids from Horned Melon Peel via Cloud Point Extraction.

Using natural plant extracts as food additives is a promising approach for improving food products' quality, nutritional value, and safety, offering advantages for both consumers and the environment. Therefore, the main goal of this study was to develop a sustainable method for extracting polyphenols and carotenoids from horned melon peel using the cloud point extraction (CPE) technique, intending to utilize it as a natural food additive. CPE is novel promising extraction method for separation and pre-concentration of different compounds while being simple, inexpensive, and low-toxic. Three parameters within the CPE approach, i.e., pH, equilibrium temperature, and equilibrium time, were investigated as independent variables through the implementation of Box-Behnken design and statistical analyses. The optimized conditions for the maximum recovery of both polyphenols and carotenoids, reaching 236.14 mg GAE/100 g and 13.80 mg β carotene/100 g, respectively, were a pH value of 7.32, an equilibrium temperature of 55 °C, and an equilibrium time of 43.03 min. The obtained bioactives' recovery values under the optimized conditions corresponded to the predicted ones, indicating the suitability of the employed RSM model. These results highlight the effectiveness of CPE in extracting bioactive compounds with varying polarities from agricultural by-products, underscoring its potential for enhancing the value of food waste and advancing sustainable practices in food processing. According to microbiological food safety parameters, the optimal CPE extract is suitable for food applications, while its storage under refrigerated and dark conditions is particularly beneficial. The CPE extract's enhanced stability under these conditions makes it a more viable option for long-term storage, preserving both safety and quality.

Recent advances in capillary electrophoresis-mass spectrometry analysis of ‍trace biomolecules

In recent years, various trace bioanalysis methods have been developed, including single-cell transcriptome analysis methods. As the sample volume and amount of biomolecules contained therein are extremely limited, development of new single-cell analysis methods require extremely high-level techniques. It is necessary to design an appropriate analysis system that integrates a highly sensitive detection system and a pretreatment protocol for minimizing sample loss, where separation method is especially important for analyzing diverse mixtures of biomolecules. Among them, capillary electrophoresis (CE) can separate biomolecules in nanoliter-scale solutions with high resolution, making it highly compatible with trace samples such as single cells. By combining with highly sensitive nano-electrospray ionization-mass spectrometry (MS), it is possible to detect nanomolar to sub-nanomolar biomolecules, which can be further improved by using online sample preconcentration methods. These highly sensitive analytical techniques have made it possible to analyze trace amounts of metabolites, proteins, lipids, etc. This review paper summarizes the research on CE-MS trace bioanalysis that has been reported to date, with a focus on single-cell analysis.

Practical Osmotic Agent for High-Degree Pharmaceutical Pre-Concentration by Organic Solvent Forward Osmosis.

Pre-concentration can reduce the total production costs in the pharmaceutical industry. Organic solvent forward osmosis (OSFO) is a suitable pre-concentration method because of its nonthermal nature, low capital cost, and potential for achieving high-degree concentrations. In a previous study, we first demonstrated a high-degree OSFO concentration. Sucrose octaacetate (SoA) in MeOH was concentrated to 52 wt% using polyethylene glycol (PEG)-400 as the osmotic agent, but the concentrated solution had a concentration of 17% PEG-400 because of the reverse solute flux. This result does not meet the typical purity standards required for pharmaceutical production, indicating the need to determine a suitable osmotic agent that can be used for practical purposes. This study proposes a practical osmotic agent for OSFO pre-concentration. First, osmotic agents were screened from a practical perspective. Polypropylene glycol (PPG)-400 was selected, owing to its low toxicity, good solubility, and low viscosity. Subsequently, the OSFO concentration was demonstrated using PPG-400 as the osmotic agent. SoA in MeOH was concentrated from 9.4 wt% to 48 wt%. The final feed solution contained only 0.04 wt% PPG-400. This result is the first demonstration of successful pharmaceutical pre-concentration using OSFO that satisfies the typical purity requirement in pharmaceutical production.

Determination of amino acids and peptides without their pre-column derivatization by capillary electrophoresis with ultraviolet and contactless conductivity detection. An overview.

This review provides an overview of recent works focusing on the determination of amino acids (AAs) and peptides using capillary electrophoresis with contactless conductivity detection and ultraviolet (UV) detection, which is the most widespread detection in capillary electromigration techniques, without pre-capillary derivatization. Available options for the UV detection of these analytes, such as indirect detection, complexation with transition metal ions, and in-capillary derivatization are described. Developments in the field of direct detection of UV-absorbing AAs and peptides as well as progress in chiral separation are described. A separate section is dedicated to using on-line sample preconcentration methods combined with capillary electrophoresis-UV.

Sustainable apple juice concentration: A fusion of pasteurization and membrane distillation

Apple juice concentrate offers significant advantages in terms of transportation and storage. However, the conventional concentration method is known for its high energy consumption and has been observed to negatively affect the quality of the apple juice. This study investigated an efficient and sustainable process combining pasteurization and membrane distillation (MD) for apple juice concentration. The effects of operational conditions on flux, membrane fouling control, product quality and energy consumption were evaluated. When a single MD was used as a pre-concentration method, apple juice could be concentrated by more than 3.65 times. The final product with a total soluble solids (TSS) content of 60 °Brix after VE re-concentration has better quality, especially in terms of acidity and total phenols content. The flux recovery rate of hydraulic washing can reach more than 96 %. The MD pre-concentration process used uncooled pasteurized apple juice as the feed reduced the energy consumption by 60.6 % compared with thermal concentration.

Application of MoS42−intercalated magnetic layered double hydroxide for preconcentration of cadmium and lead from water samples

A MoS42−-intercalated magnetic FeMgAl layered double hydroxide (Fe3O4@MoS42−-FeMgAl LDH) nanocomposite was synthesised via hydrothermal assisted exfoliation. The material was applied as the adsorbent for extraction, preconcentration and removal of cadmium ions (Cd2+) and lead ions Pb2+ from wastewater and river water. The structural properties and morphologies of the adsorbent were determined by transmission electron microscopy (TEM), scanning electron microscopy coupled with energy dispersive spectroscopy (SEM/EDS), Fourier transform infrared spectroscopy (FTIR), zeta potential and X-ray diffraction (XRD). The parameters influencing preconcentration and the adsorptive removal process were optimised using the central composite design (CCD) method. The concentration of Cd2+ and Pb2+ in the samples was determined using inductively coupled plasma optical emission spectrometry (ICP-OES). The preconcentration method developed in the study was ultrasound-assisted magnetic solid phase extraction (UA-MSPE). Under optimum conditions, linearity was 0.1-800 µg/L with a correlation of determination (R2) of 0.9987. The uncertainty of the slope and the uncertainty of the intercept were both lower than the uncertainty of the calibration indicating good precision, selection of standards closest to a blank and wide range. Precision expressed as %RSD for both intra-day (n = 27) and inter-day (n = 9) were found to be 3.3 % and 7.0 % respectively. The limit of detection (n = 10) and limit of quantification (n = 10) of the UA-MSPE/ICP-OES were 0.03 µg/L and 0.10 µg/L. The developed method was applied for real sample analysis. Herein, influent wastewater was reported as 1.77 ± 0.14 and 6.53 ± 0.33 ug/L for Cd2+ and Pb2+, respectively while the effluent was reported at 0.96 ± 0.07 and 2.57 ± 0.13 ug/L, respectively. River water samples were found to be 0.45 ± 0.01 and 1.23 ± 0.04 ug/L, respectively proving that the adsorbent is capable of preconcentrating the target analytes.

Dispersive Liquid–Liquid Micro Extraction: An Analytical Technique Undergoing Continuous Evolution and Development—A Review of the Last 5 Years

Over the years, monitoring the quality of the environment, food, and human health has emerged as a fundamental objective of scientific investigation. Various analytical techniques have been devised and continually refined to ensure a high standard of living. Among these methods, dispersive liquid–liquid microextraction (DLLME) stands out as a widely used approach to conduct analytical evaluations and pre-concentrations of chemical compounds. This review provides an account of the evolution of DLLME over the past five years (2018–2023). The Scopus search engine was used to search for scientific articles. The selection of relevant articles was carried out according to the criteria described below. Emphasis was placed on the evolution that DLLME has undergone over the years by integrating it with other pre-concentration methods in order to amplify the concentration factor and improve analytical precision. Factors such as solvent dispersion and extraction, dispersion formation, and the recovery of the solvent containing the extracted analytes were examined. Additionally, derivatization systems were evaluated to refine both qualitative and quantitative analysis. These efforts aim to introduce methods that are safe for operators, environmentally friendly, and economical. This review evaluates the advantages and disadvantages displayed by the evolution of this technique in the phases before, during, and after its application.

A facile and green alternative method for preconcentration of triazole fungicides using fabrication of melamine sponge anchoring with Ni/Al-LDH adsorbent followed by HPLC analysis

In this work, a facile and green alternative method for preconcentration of triazole fungicides (TFs) using melamine sponge anchoring with nickel/aluminium layered double hydroxide (Ni/Al-LDH-MS) as adsorbent for micro-solid phase extraction (micro-SPE). LDHs was prepared by nickel (II) nitrate hexahydrate and aluminium nitrate nonahydrate. The adsorbent was simply synthesized under facile condition via anchoring of Ni/Al-LDH on the pore of melamine sponge (MS). The material was applied for extraction of triazole fungicides prior to HPLC analysis. Under the chosen conditions, the proposed method provides wide linearity between 0.09 –1.00 µg L-1 with coefficients for determination (R2) of greater than 0.99. Low detection limits of 0.03 µg L-1 for all analytes were achieved. The satisfactory recoveries of the real samples at three different concentration levels were obtained in the range of 64.5 % to 105.6 % with RSDs lower than 7.5 %. The findings highlight the importance of developing efficient adsorbents for the enrichment of trace triazoles in surface water, soymilk, honey and egg yolk matrices. Eco-friendliness was confirmed by Analytical Eco-scale and Analytical GREEnness metric (AGREE) which shown that the recommended analytical procedure is more green than previous methods.

Can preconcentration of cassiterite from its pegmatite ore reduce processing costs and improve operational sustainability?

Different concentration techniques were evaluated for preconcentration of a mineral ore at a coarser size to avoid energy and resource wastage. Specifically, the aim was to reduce milling costs and energy required in the beneficiation of tin. In this study, cassiterite (0.17% Sn) was the mineral of interest in a pegmatite ore body associated with quartz (SiO2 > 60%) and alumina (Al2O3 > 20%). Three concentration techniques, namely dense media, shaking table, and flotation, coupled with characterization analysis, were used to assess the concentration response. The results confirmed that particle size and mineral liberation impact the separation process. High recovery and grade were obtained with gravity concentration methods (dense media and shaking table) for coarser (+300 to +212 μm) and intermediate (+150 to +53 μm) particle sizes. Lower recovery and grade were identified for much finer sizes (-53 to -38 μm). Flotation produced a high-grade product at a relatively low recovery and appeared to be only applicable to finer grains. Separation efficiency based on Schulz's equation measured a segregation performance of 74.8% for dense medium separation and 60.7% for the shaking table for the coarse and intermediate particle sizes. Flotation only achieved a separation of 30%-40%. The results suggest that use of dense media separation as a rough preconcentration method prior to further grinding, and the utilization of a more advanced concentration technique for mineral recovery and upgrade, constitute a successful approach to improve process economics.

Optimized Preconcentration Method Using Ionic Liquid Ferrofluid for Ultra-Trace Determination of Cr(VI) in Drinking Water by Inductively Coupled Plasma Mass Spectrometry

Optimized Preconcentration Method Using Ionic Liquid Ferrofluid for Ultra-Trace Determination of Cr(VI) in Drinking Water by Inductively Coupled Plasma Mass Spectrometry

Dispersive liquid–liquid microextraction based on deep eutectic solvent followed by high-performance liquid chromatography-ultraviolet for imidacloprid, hexythiazox, and fenpyroximate determination: Experimental design approach

Dispersive liquid–liquid microextraction (DLLME) of three insecticides, including imidacloprid (I), hexythiazox (H), and fenpyroximate (F), was performed as an efficient method for separation and preconcentration using a deep eutectic choline chloride-acetic acid solvent (DES) followed by determination by high-performance liquid chromatography-ultraviolet (HPLC-UV). The experimental design approach (central composite design) was used to optimize the effective parameters. According to the results, the addition of salt had no significant effect on extraction among the four parameters studied, including pH, DES volume (dispersive solvent), salt concentration, and chloroform volume. The optimized conditions in the DES-DLLME-HPLC-UV method were pH = 6.00, 100 µL DES, and 138 µL chloroform. Under optimized conditions, a wide linear range of 1.00 × 10−3-20.00 µg mL−1 was obtained for three insecticides. The enhancement factors were 11.22, 58.04, and 39.65 and the limit of detection of 3.00 × 10−4, 2.00 × 10−4, and 2.00 × 10−4 µg mL−1, and the limit of quantification of 9.00 × 10−4, 8.00 × 10−4, and 6.00 × 10−4 µg mL−1 were also determined for imidacloprid, hexythiazox, and fenpyroximate, respectively. The presence of other components including benomyl, dimethoate, mancozeb, captan, thiodicarb, carbendazim, and oxyfluorfen, showed no significant interference effect on the determination of three insecticides up to 50-fold. The method is eco-friendly benign, sensitive, precise, and straightforward as shown by the results.

Statistical optimization of two-phase hollow fiber liquid-phase microextraction for preconcentration of strychnine in the clinical and postmortem biological fluids prior to gas chromatography-mass spectroscopy detection

Strychnine is a herbal alkaloid whose role in military and doping applications as well as the nervous system stimulation and cancer therapy has been confirmed. Because of its lethal nature, even at low dosages, developing a rapid, sensitive, and accurate method for determination of this alkaloid in the clinical and postmortem samples is of great importance. In this study, a green analytical method based on the two-phase hollow fiber liquid-phase microextraction (HF-LPME) combined with gas chromatography-mass spectrometry (GC–MS) was presented for preconcentration and determination of strychnine in biological fluids. A Box-Behnken design (BBD) with three variables, including extraction time (30–90 min), solution pH (9.0–11.0), and ionic strength (1.0–3.0 mol/L) was employed to maximize the extraction efficiency of strychnine by 1-octanol. The results were satisfactorily fitted to a quadratic response surface model (R2 = 0.979, F = 34.4) which predicted the optimum conditions of operation (extraction time of 89 min, pH = 11.0, and ionic strength of 3.0 mol/L). Based on the results, the studied variables and the interaction between pH and extraction time showed highly significant effects on the process efficiency. The calibration curve was linear in the concentration range of 2.7–1000.0 µg/L (R2 = 0.9978) and the limits of detection and quantitation were found to be 0.8 and 2.7 µg/L, respectively. Moreover, the method was successfully applied to different real samples, including urine (clinical sample), bile and stomach contents, blood, and vitreous humor (postmortem samples), and relative recoveries were obtained in the range of 93.8 % to 99.8 %. The findings of this study suggest that the environmentally friendly HF-LPME method which uses a small amount of a chlorine-free solvent, is a sensitive, cost-effective, simple, and efficient preconcentration method for trace determination of strychnine in biological fluids.

Determination of phenytoin at trace levels in domestic wastewater and synthetic urine samples by gas chromatography-mass spectrometry after its preconcentration by simple liquid-phase microextraction.

This work presents a sensitive and accurate analytical method for the determination of phenytoin at trace levels in domestic wastewater and synthetic urine samples by gas chromatography-mass spectrometry (GC-MS) after the metal sieve-linked double syringe liquid-phase microextraction (MSLDS-LPME) method. A metal sieve was produced in our laboratory in order to disperse water-immiscible extraction solvents into aqueous media. Univariate optimization studies for the selection of proper extraction solvent, extraction solvent volume, mixing cycle, and initial sample volume were carried out. Under the optimum MSLDS-LPME conditions, mass-based dynamic range, limit of quantitation (LOQ), limit of detection (LOD), and percent relative standard deviation (%RSD) for the lowest concentration in calibration plot were figured out to be 100.5-10964.2 μg kg-1, 150.6 μg kg-1, 45.2 μg kg-1, and 9.4%, respectively. Detection power was improved as 187.7-folds by the developed MSLDS-LPME-GC-MS system while enhancement in calibration sensitivity was recorded as 188.0-folds. In the final step of this study, the accuracy and applicability of the proposed system were tested by matrix matching calibration strategy. Percent recovery results for domestic wastewater and synthetic urine samples were calculated as 95.6-110.3% and 91.7-106.6%, respectively. These results proved the accuracy and applicability of the proposed preconcentration method, and the obtained analytical results showed the efficiency of the lab-made metal sieve apparatus.

Green-AgNPs modified membrane for monitoring mercury ions in cosmetic sample using pre-concentration assisted voltammetry technique

The carcinogenic and teratogenic properties of mercury have driven the necessity for the development of highly sensitive methods capable of detecting even trace amounts of the element. In response to this need, an analysis technique has been established for mercury ions, employing preconcentration methods utilizing filter paper modified by silver nanoparticles (AgNPs) and 3-Amino propyl trimethoxy silane (APTMS). This method allows for the detection of mercury ions through the voltammetry technique, ensuring accurate and reliable results. Upon conducting analyses using this method, it was found that the tested cosmetic samples were free from Hg2+ ions, indicating the absence of mercury contamination in the cosmetics under investigation. The correlation coefficient derived from the graph of the analysis results stands at an impressive value of 0.9976, affirming the strong relationship between the measured concentrations and the actual mercury content. Additionally, the method demonstrates excellent sensitivity, as evidenced by the low limit of detection (LOD) value of 0.0483 mM and the limit of quantification (LOQ) value of 0.1611 mM.

CoSn(OH)6 nanocubes as a solid sorbent for the effective preconcentration of copper ions in cinnamon (Cinnamomum zeylanicum) extract

This study was aimed at developing a simple and efficient CoSn(OH)6 nanocubes-based preconcentration method for the preconcentration of copper ions from cinnamon extracts for determination by flame atomic absorption spectrometry. The cube-shaped sorbent was synthesized using the simple stoichiometric co-precipitation method under ambient conditions. Experimental factors of the method were evaluated with a comprehensive optimization approach to maximize the extraction efficiency for the analyte. Under the optimal conditions, the limit of detection (LOD), limit of quantitation (LOQ), and linear dynamic range were recorded as 0.98 µg/L, 3.28 µg/L, and 4.0–75 µg/L, respectively. The enhancement factor was calculated as 101.6-fold by comparing the LODs of the optimized and direct analysis systems. Percent recoveries were found to be within an acceptable range (77.6–115 %), with high repeatability using matrix matching calibration strategy. Results validated the proposed method as a highly efficient extraction approach for the monitoring of copper ions in herbal cinnamon extracts.

Magnetic stirring in syringe dispersive liquid-liquid microextraction as an effective method for preconcentration of tartrazine dye from food samples: A multivariate analysis approach

A new, rapid, simple, and sensitive preconcentration method and Spectrophotometry determination technique have been presented for the microextraction and determination of trace amount of Tartrazine dye in food samples. In the present system, which is called “Magnetic stirring in syring dispersive liquid-liquid microextraction"(MSIS- DLLME), a cloudy state result is formed in a homemade glass syringe by magnetic agitation. In the MSIS-DLLME system, Tartrazine colour was uprooted into an organic detergent (Toluene) after many twinkles. Subsequently, the organic detergent which was placed on top of the result was transferred into a narrow neck by moving the piston overhead. The effective parameters on the extraction recovery were studied and optimized by Central Composite design (CCD). Under the optimum conditions, the estimation cure is direct in the range of 0.1–1(μg L− 1). The limit of detection (LOD), relative standard divagation and enrichment factor were 0.03 μg L −1, ±4.6 (n = 10) and 166, independently. The advanced system was successfully applied for microextraction of Tartrazine in food samples.

Analysis of atorvastatin in environmental waters: Validation of an electrochemical molecularly imprinted polymer sensor with application of life cycle assessment

The widespread presence of pharmaceuticals in wastewater effluents after treatment stands as a significant challenge faced in the field of wastewater management and public health. Governments and the scientific community have worked to meet this urgent need for effective solutions. Nevertheless, the development of detection strategies for pharmaceutical monitorization capable of delivering rapid, on-site, and sensitive responses remains an ongoing necessity. In this work, the performance of a previously developed molecularly imprinted polymer (MIP) based electrochemical sensor for detecting atorvastatin (ATV) in wastewater effluents and surface waters is presented. A simple preconcentration method followed by electrochemical measurements by differential pulse voltammetry (DPV) in 0.1 M phosphate buffer (pH = 7), was implemented. The analytical results were validated with those obtained on a set of 16 water samples by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Additionally, a life cycle assessment (LCA) was conducted to compare the environmental impact of both methodologies. The results obtained demonstrated that ATV detection using MIP-sensor was reliable when compared to the results found by UHPLC-MS/MS presenting a robust linear correlation coefficient of 0.843. The LCA results show that the novel MIP-sensor technique has lower associated environmental impacts than UHPLC-MS/MS, when the current analytical protocol for pharmaceuticals detection is applied. These findings highlight the potential of the developed MIP-sensor as an eco-friendly analytical tool for routine analysis and point-of-care monitoring of ATV in WWTP wastewater and surface water samples.