Objective: The aim of this work was to determine the rates of prevalence and recurrence of TVT infection in owned dogs, as well as the distribution of the disease, in Cuajinicuilapa, Guerrero, Mexico. Methods: This work was an observational, descriptive, longitudinal study. A TVT diagnosis was performed in all dogs in the community based on clinical records, a physical examination, and a cytological evaluation, and further confirmed by histopathology. Tissue samples were surgically obtained for histopathological analysis and, when required, to remove tumors and give treatment with vincristine; a GPS system was used to identify possible spatial groupings. All cases were re-evaluated one year after. Results: A prevalence of 5.15% was found in 1047 dogs (512 females and 535 males; 25 positive females and 29 positive males). TVT infection was more frequent in 2–3-years-old dogs. A spatial aggregation pattern was observed in the local cases. One year after, no lesions were found in the 29 animals treated. Conclusion: TVT is a common disease, and its distribution suggests its aggregation in certain areas of the locality. Further studies on the dynamics of dog populations in small cities are required.