ABSTRACT Indoor environmental quality has been associated with the health and wellbeing of building occupants; nevertheless, there is limited evidence in this regard for Latin American schools. This research aimed to characterize indoor environmental quality in public and private preschools in an Andean city in Ecuador. Data collection comprised onsite monitoring for the thermal-humidity microclimate of 90 classrooms in 30 preschools in Cuenca-Ecuador (March-August 2018). Infrared thermography and direct observation were applied to determine dampness. Classrooms seemed to be inadequate thermal-humidity microclimates; only a few maintained a comfortable temperature (6%) and relative humidity (11%) throughout the shift. When comparing public and private schools, in private schools, temperatures below the comfort range (61.3% in private schools vs 31.4% in public schools, p<0.001) and relative humidity measures above the comfort range were more frequent (74.3% in private schools vs. 58.6% in public schools, p<0.001). Hollow blocks were the primary construction material in private and public schools. Sixty-four per cent of private schools operated in adaptive, reused buildings, vs 19% in public schools (p<0.05). Infrared thermography confirmed dampness in 26% (n=23) of the classrooms in the covering structures indoors (15% in public vs 33% in private schools, p<0.05). This research reveals the urgent need to develop specific regulations and control mechanisms for building sustainable and healthy environments for preschools in Ecuador.