Background: Sexual attitudes are a central concept in the study of sexuality and may largely influence the decision-making of the youth to engage in premarital sexual relations. Objectives: The present study aimed to investigate the sexual attitudes (permissive/conservative) of Iranian students. Methods: This study was conducted on 280 students (170 females and 110 males) who were selected via multistage random sampling from the students of Ahvaz Jundishapur University of Medical Sciences in Ahvaz, Iran. Data were collected using the short-form sexual attitude scale. Data analysis was performed in SPSS version 22 using the factorial analysis of variance. Results: A significant difference was observed between the male and female students in terms of sexual attitudes as the sexual attitude of the female students was conservative, while the sexual attitude of the male students was permissive. In addition, sexual attitudes differed significantly between age groups as the students aged 18 - 22 years had conservative sexual attitudes, while those aged 23 - 26 years had permissive sexual attitudes. Conclusion: The results of this survey could guide psychologists and counselors in helping students with the issues regarding premarital sex and the prevention of high-risk sexual behaviors.