The advanced MEdium Resolution Spectral Imager (MERSI II) is a major instrument onboard the Chinese FengYun 3D satellite, which was launched in November 2017. Extensive measurements were performed during MERSI II prelaunch testing to ensure effective characterization for on-orbit calibration. This paper gives a brief overview of the prelaunch performance testing conducted for MERSI II, as well as its improvements in terms of instrument design compared to MERSI I. The prelaunch calibration methodology and radiometric performance are detailed, including dynamic range, signal-to-noise ratio, noise equivalent differential temperature, linearity, and response uniformity. The assessment results indicate that most bands perform effectively with mirror specification noncompliances in a few reflective solar bands (RSBs). In addition, investigation of the stability and uniformity of the spherical integrating source indicates that they have a critical impact on the performance assessment and prelaunch calibration of RSBs. The temperature-dependence features of thermal emissive bands' performances are also discussed in terms of their sensitivity to the operating temperature of the focal plane assembly and instrument circumstance. This paper also shows that the self-stability of the calibration source and the representation of the assessment methods are important as they affect the results of instrument performance evaluation.