Aflatoxins pose a serious problem to maize grains, rendering them unsuitable for human and livestock consumption. This study assessed farmers’ attitude toward, and knowledge and perceptions of, the nature, causes and use of biological technologies in aflatoxin control in Embu, Machakos, Makueni and Kitui Counties of Kenya. A total of 480 households were randomly selected from two districts per county using a GPS system. The tools used in the survey included pre-tested semi-structured questionnaires, focused group discussions, key informant interviews and secondary data exploration. The information collected included crop production constraints, existing aflatoxin management strategies, farmers’ experiences with use of atoxigenic strains of Aspergillus and other household farm production activities. The study found that farmers were largely aware of the aflatoxin problem and its negative effects, factors that contribute to its occurrence, and available options to address it. The main pre-harvest aflatoxin control technologies used were crop rotation, irrigation, use of resistant crop varieties and pest control. The main post-harvest aflatoxin control technologies used were proper storage, drying, sorting, and use of pesticides to manage pests. Modern aflatoxin control technologies such as ammoniation and use of hydrogels were not in use mainly due to unavailability, high cost and safety concerns (especially regarding grains meant for human consumption). Effective management of aflatoxin contamination requires that farmers are aware of the problem and use a combination of strategies targeting the crop before and after harvesting, and understanding farmers’ perceptions and knowledge is a vital step to finding a local solution for aflatoxin management. The study recommends the strengthening of existing public extension service system to enable it deliver up-to-date information, through a variety of channels, on aflatoxin and its management to farmers in a more effective and timely manner. Key words: Aflatoxin, Aflasafe, farmers, perceptions, Kenya.