Introduction: Schools are the valuable place for promotion of oral health, and primary school teachers have a unique position to influence oral hygiene practices in children. The aim of this study was to evaluate the knowledge, attitudes, and practices of primary school teachers of Tehran, Iran. Materials and Methods: A cross-sectional questionnaire-based survey was conducted among 195 primary school teachers of Tehran, Iran. The multiple-choice questionnaire regarding dental disease prevention is comprised of ten questions on knowledge, seven on practice, and five on attitude. Distribution of individuals in terms of variables was expressed as numbers and percentages, and a logistic regression test was used to investigate the relationship between variables. Results: 61.6% of the primary school teachers in Tehran had good knowledge on oral health, but their attitude, especially about their role in teaching oral health, is very unfavorable. Teachers with higher education and participation in continuing educational courses had a positive effect on their performance. Conclusion: All teachers should be trained at regular intervals, given the importance of oral health and raising awareness about improving oral health for their students.
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