Background: Secure supply of safe blood components, based on voluntary, non-remunerated blood donation, is an important national goal to prevent blood shortages. Nowadays, in many low- and middle-income countries, blood supply is critically inadequate. Sub-Saharan Africa, which has the highest maternal mortality rate in the world of 510 deaths per 100000 live births, also has the lowest blood donation rates. The donated blood plays a big role during surgery, accident, delivery cases and in many bleeding cases.
 The aim of this work is to assess knowledge, attitude, and practice and associated factors of adult population towards blood donation in Jigjiga town, Eastern Ethiopia, 2019. A total of20 kebeles, six was selected by simple random sampling. The sample size was calculated by using EPI info version 7.2by considering power of 80%, confidence level of 95%, by taking the comprehensive knowledge of the study Participants towards voluntary blood donation of 43.5% from study conducted in Harartown, giving totalsample size of416. 
 Result: About 249(61.2%), 318 (78.1%), and 77 (18.9%) study participants had adequate knowledge, good attitude and experience of blood donation. Those who were in college and higher education were 2.034 higher odds of knowledge towards blood donation relative to those in secondary and primary education. Study participants who were government employees and daily workers were 2.259(1.103, 4.629) times more knowledgable to housewife, students. Study particpants age range between 18-30 have less knowledge towards blood donation 0.408 times. Participants who attended college and higher education were more likely to have good attitude towards blood donation. Those respondents who are college and higher education and secondary were 2.450 times more likely, 2.359to have good attitude towards blood donation compared to primary school 2.161 respectively. Participant’s sex, education, and occupational status were found to be significantly associated with practice of blood donation. Being NGO worker is more likely to donate blood 8.167 higher than others. Male participants was more vulnarable to donate blood 5.245 times higher than female for blood do nation. In addition to this, those respondents who are graduated college and higher education were more likely to donate blood than others 3.247.
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