Study’s Excerpt/Novelty This study reveals a significant Epstein-Barr Virus (EBV) prevalence among pregnant women attending Cottage Hospital in Ilorin, with 95.69% of participants testing positive for anti-EBV IgG antibodies. Notably, the highest prevalence was observed among younger women, those in the first trimester, individuals from polygamous families, and those with lower educational levels. These findings underscore the need for increased community awareness and improved personal hygiene practices to reduce EBV infection rates and associated congenital defects. Full Abstract Epstein-Barr Virus (EBV), a member of the Herpesvirus family, has been observed to be increasing among pregnant women, leading to congenital defects. This study aimed to determine the prevalence of EBV infection among pregnant women attending Cottage Hospital in Ilorin. A total of 45 pregnant women were tested, and questionnaires were administered to volunteer subjects after obtaining their informed consent. Approximately 5 ml of blood was aseptically collected via venous puncture from the antecubital fossa and dispensed into EDTA containers. Plasma was separated after centrifugation of the blood, and the obtained plasma was screened for anti-EBV IgG antibodies using ELISA techniques. Results revealed that 43 subjects (95.69%) tested positive for EBV, while 2 (4.4%) tested negative. Among different age groups, women aged 19-20, 26-30, and 36-40 showed a prevalence rate of 100%, with the lowest prevalence of 88.8% recorded among the 31-35 age group. Regarding the pregnancy stage, 100% was seen among first-trimester participants, while the lowest prevalence of 92.3% was noted during the third trimester. The majority of respondents were married, with those from polygamous families and single individuals showing a prevalence of 100%. Participants with primary and secondary education levels displayed the highest prevalence, while those with tertiary education had the lowest. Muslims had the highest prevalence, whereas Christians had the lowest. Moreover, regarding occupation, the unemployed, artisans, and students had the highest prevalence, while civil servants and businesswomen had the lowest prevalence. In conclusion, the results from this study highlight a notable prevalence of EBV (IgG) antibodies among pregnant women. These outcomes emphasize the necessity of raising awareness in the community about the implications of EBV infection and the Significance of maintaining proper personal hygiene to mitigate the chances of infection and spread