Understanding paint structures at the nanoscopic level can address key questions related to artistic techniques, paint formulation, and long-term preservation of artworks. This involves examining spatial chemical complexity, the formation of molecular networks, and interactions between organic and inorganic constituents. Depending on the paint preparation methods, proteins and drying oils, the most common binders in traditional artistic practices, can be integrated to produce paints with diverse structures and nanoscale chemical intricacies. In this study, we utilize atomic force microscopy-based infrared spectroscopy (AFM-IR) to investigate the spatial chemical complexity and reaction pathways of organic species in artists' paints, including oil, tempera, and mixed-media tempera grassa. By analyzing these paints at the nanoscale, we established connections between their structural organization, chemistry, and formulation.
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