Background: First aid is the assessments and interventions that can be performed by medic (or by the injured person) immediately with minimal or no medical equipment. Training is necessary to equip the first aid provider with the knowledge, skill, and confidence to attend to an emergency. Objectives: To assess the knowledge and practices of primary school teachers in Hay Al-Salaam Al-Sakani about the first aid. Methods: A cross-sectional study with analytic element was conducted in Baghdad during the period from February to August 2018. Eight schools were choose randomly, 145 teachers who were work in choosing schools and accept to involve in this study. A structure questionnaire depending on the Iraqi Red Crescent guideline for First Aid, and American College of Emergency Physician's First Aid manual 5th edition was used. Results: One hundred and forty five teachers were involved in this study, 44.1% of them aged 40–49 year, most of them were females (93.8%), graduated institute (63.4%), married (77.9%), had one to three children (65.5%), had 11–18 years teaching experience (30.3%). Only 30.3% applied first aid previously, most of the teachers (71%) did not involve in first aid training courses. Teachers age had statistically significant association with first aid general-knowledge, knowledge about poisoning, fractures, and burn. Their educational level had statistically significant with knowledge about coma, epilepsy, fractures, mouth injuries, and burn. Conclusion: First aid knowledge in general and in each type of school accident was good except knowledge in asphyxia and electric shock were fair knowledge; most of them were not involved in training courses for first aid, or applied first aid.