Ppm Indoleacetic Acid Research Articles

The Effect of IAA and BAP on Root Induction of Cattleya Orchids

Orchid is an ornamental plant commodity that has an important meaning in international trade. Until now, orchids are still the center of attention of farmers and lovers of ornamental plants, because of their bright potential as cut flowers and potted plants. The popular and widespread type of orchid is Cattleya sp. The Cattleya orchid is also called the queen of orchids because of its colorful flowers. Cattleya sp. orchids take a long time to propagate by seed, about 4 to 7 years, so other methods are needed to deal with them. Tissue culture is a method for isolating plant parts such as cells, tissues or organs, and cultivating them in an aseptic environment. The purpose of this study was to determine the optimal interaction of growth regulators indole acetic acid (IAA), benzyl amino purine (BAP), and their interactions with the root induction of Cattleya sp. Cytokinins interact with auxin in determining the direction of cell differentiation. In this research method, differences in concentrations of indole acetic acid (IAA: 0, 2, 4, 6 ppm) and concentrations of benzyl amino purine (BAP: 0, 0, 5, 1 ppm) were carried out. Parameters observed were the number of roots and root length. Data were analyzed descriptively and inferentially using ANOVA. The highest number of roots was obtained in the treatment medium containing 0.5 ppm BAP and 6 ppm IAA, which was 6.33. The highest root length was found in the B0I4 treatment (BAP 0 + IAA 4 ppm). The results of the analysis of the interaction variance between the IAA treatment and the BAP treatment were 0.005, meaning that there was a significant effect of the combination of IAA and BAP on the leaf length of Cattleya sp.

Effect of Plant Growth Regulators on the Morphology, Flowering, Maturity, Yield Attributes, and Yield of Lentil (Lens culinaris L. Medik.)

Comprehensively, nutrient deficiencies in humans and animals are a quiet epidemic in many underdeveloped nations. Nutrient deficiencies in humans and animals are a global problem in most developing countries. Lentil is one of the most important and nutritious Rabi pulse in India. It’s a leguminous crop that improves the soil fertility through biological nitrogen fixation. In this contest an experiment was conducted to study the enhancement of plant growth, chlorophyll content, flowering, yield and seed protein content by plant growth regulators in DPL-62 lentil (Lens culinaris L. Medik) variety during two Rabi seasons of 2019-20 and 2020-21. The field experiment was laid out in randomized block design (RBD). Five growth regulators viz. triiodobenzoic acid (TIBA), gibberellic acid (GA3), cytokinin, indole acetic acid (IAA), and naphthaleneacetic acid (NAA) were used in Eleven treatments were tested T1: Control, T2: 25 ppm TIBA, T3: 50 ppm TIBA, T4: 25 ppm GA3, T5: 50 ppm GA3, T6: 5 ppm Cytokinin, T7: 10 ppm Cytokinin, T8: 25 ppm IAA, T9: 50 ppm IAA, T10: 25 ppm NAA, T11: 50 ppm NAA. The results revealed that the maximum plant height (38.12 & 38.15 cm at harvest) was obtained by the 50 ppm GA3 followed by 50 ppm IAA (37.00 & 38.10 cm at harvest) spray at all stages. However, the 50 ppm TIBA showed better results (per plant) viz., number of branches (15.00, 15.33 at 110DAS), leaf area (86.50, 86.32 at 110DAS), number of pods (160.20, 160.37), pod setting (65.45, 65.50%), seed weight (2.02, 2.10g), test weight (27.32, 27.39g) and seed yield (1500.00, 1510.00 kg ha-1) of lentil as compared to all other treatments. The maximum days of flowering (67.50, 67.85) and days of maturity. On the basis of the observed results the growing of DPL-60 lentil variety with foliar applications of 50 ppm TIBA is recommended for the farmers of the test area for greater production and profitability.

Effects of Plant Spacing and Indole-3-Acetic Acid on Vegetative Growth, Flowering and Yield Features of Gladiolus (<i>Gladiolus palustris</i>)

The experiment was carried out at the Horticulture Farm of Bangladesh Agricultural University (BAU), Mymensingh during the epoch from November, 2020 to April, 2021 with the aim to observe the outcomes of plant spacing and indole-3 acetic acid (IAA) concentrations on the vegetative growth, flowering and yield features of gladiolus. The research was two factors, Factor A: three plant spacing viz. S₁ = 15 cm x 25 cm, S₂ = 20 cm x 25 cm, S₃ = 25 cm x 25 cm and Factor B: different concentrations of indole-3-acetic acid (IAA) viz. I₁= IAA 0 ppm, I₂=100 ppm and I₃=150 ppm. It was Randomized Complete Block Design with three replications. The 25 cm × 25 cm spacing showed best performance in respect to plant height (69.88 cm), yield of flower (162.22 t ha^-1), yield of corm (15.65 t ha^-1), and yield of cormel (14.32 t ha^-1) than the other spacing. Application of IAA at a rate of 150 ppm was initiated to be excellent for plant height (68.51 cm), yield of flower (162.67 t ha^-1), yield of corm (15.57 t ha^-1) and yield of cormel (13.93 t ha^-1). Regarding the combined effect of treatments, 150 ppm IAA with wider plant spacing was found to be best for plant height (70.73cm), yield of flower (166.67 t ha^-1), diameter of corm (7 cm) and weight of cormel per plant (81.87gm). Considering the above findings, it was apparent that the combination of 25 cm × 25 cm spacing with 150 ppm IAA application was suitable for better growth, flowering and yield/production of gladiolus.

LYCOPENE CONTENT IN SEVERAL AGES OF TOMATO CALLUS (Lycopersiconesculentum Mill. cv. Rampai)

Tomato (Lycopersiconesculentum Mill. cv. Rampai) is rich in lycopene, a carotenoid group compound with many biological activities, one of which is as an antioxidant. Conventional tomato cultivation is not optimal due to the susceptibility of tomato plants to pests and diseases, even though the need for lycopene continues to increase. Plant tissue culture method can be one solution for lycopene production. Callus tomato cv. Rampai was initiated from cotyledons on MS medium (Murashige& Skoog) with the addition of 1 ppm BA (benzyladenine) and 1.5 ppm IAA (indole-3-acetic acid). Callus aged one week to four weeks were harvested and lycopene was extracted by maceration method using hexane:acetone (9:1) as solvent. The presence of lycopene in callus was confirmed by TLC (thin-layer chromatography) (retention factor value/Rf = 0.56) and UV-Vis spectrophotometry (wavelength that gives maximum absorbance = 472 nm). Lycopene content in tomato callus increased with increasing callus age and was directly proportional to the callus growth index. The highest lycopene level was found in callus aged 4 weeks, which was 0.3094 mg/100 g dry weight callus.

PENGARUH KOMBINASI IAA (Indole-3-Acetic Acid) DAN BAP (6-Benzylaminopurine) TERHADAP INISIASI TANAMAN VANILI (Vanilla planifolia Andrews)

Provision of Vanilla (Vanilla planifolia Andrews) with traditional cultivation often has problems, including the availability of unhealthy seeds due to disease caused by Fusarium oxysporum. This disease can thwart vanilla plantations up to 85% because the pathogen can infect all parts of the vanilla plant making it difficult to control efforts. Alternative efforts were made to overcome this problem, namely through the initiation process in tissue culture by combining the hormones IAA (indole-3-acetic acid) and BAP (6-benzylaminopurine). This study aims to determine the effect and the maximum concentration of the combination of IAA and BAP on the initiation of vanilla plants. This study was conducted based on a completely randomized design (CRD) with 5 treatments and 3 repetitions. This treatment consisted of: A (MS + 0.1 ppm IAA + 1 ppm BAP), B (MS + 0.2 ppm IAA + 1 ppm BAP), C (MS + 0.3 ppm IAA + 1 ppm BAP), D (MS + 0.4 ppm IAA + 1 ppm BAP), and E (MS + 0.5 ppm IAA + 1 ppm BAP). Observations were made after 40 days from the planting process. The results showed that the concentration of the combination of IAA and BAP can affect the growth and organogenesis of the initiation of vanilla plants (Vanilla planifolia Andrews). Treatment C (MS + 0.3 ppm IAA + 1 ppm BAP) was the best concentration from this study, with average values: number of shoots (1), shoot length (1.73 cm), number of roots (1), and number of leaves (1.33).

Caracterización de bacterias rizosféricas aisladas de suelo cultivado con caña de azúcar en el estado de Tamaulipas, México

The state of Tamaulipas, Mexico, has two important sugar mills, with plantations of sugarcane (Saccharum officinarum L.) of more than 50 years, the objective of the study was the identification and bacterial characterization in the production of indole-3-acetic acid (IAA), the solubilization of phosphorus and plant growth of bacterial isolates from the rhizosphere of sugarcane. The isolation and morphological characterization were in 30 rhizosphere soil samples of the sugarcane variety CP 72-2086 using the Luria-Bertani media, Congo Red Agar and Potato Dextrose Agar. The molecular characterization was with the 16S rRNA gene and the bacterial inoculation consisted of seedlings of the variety CP 72-2086. 121 strains (38 species) were isolated, being Bacillus sp. most frequently, 14 species were positive for phosphorus solubilization: Bacillus sp. (6), Pseudomonas spp. (5), Paenibacillus (2) Streptomyces venezuelae (1) stand out. The greater phosphorus solubilization was Pseudomonas mediterranea (21.6 mm). Nine bacteria showed production close to 5 ppm IAA: Bacillus aryabhattai (6 ppm), Bacillus pumilus (5.8 ppm) and Ensifer adhaerens (5.6 ppm). Bacillus megaterium showed a higher percentage of chlorophyll and foliar nitrogen. In the present analysis, 38 bacterial species associated with the rhizosphere of the sugarcane variety CP 72-2086 were identified, so these results showed the potential to select native bacteria that have the ability to stimulate plant growth of the variety CP 72-2086.

Influence of different treatments and techniques on rooting behaviour of Rhododendron arboreum Sm. In Indian Himalayas

Rhododendron arboreum Sm. a wild flowering tree is on the verge of extinction from natural habitat due to overexploitation of its flowers and slow regeneration rate. The objective of this research was to explore the effective method for propagation of this species vegetatively, therefore, to maintain its genetic identity and population. In this context, effectiveness of vegetative propagation methods like FRI-Wire Technique and Air Layering in combination with root promoting hormones such as Indole 3- Butyric Acid (IBA), Indole Acetic Acid (IAA) and Napthelene Acetic Acid (NAA) at concentration of 1000 ppm (ppm), 2500 ppm and 5000 ppm each were evaluated through well designed experiments. Air Layering method failed to produce roots on this species, whereas, FRI-Wire Technique showed a remarkable success for rooting in the month of April with hormones IAA and NAA at 1000 ppm and 2500 ppm concentrations and no response from IBA. Branch sizes of length 45--51 cm, when treated with 2500 ppm IAA concentration performed significantly better and produced 66.66% rooting in comparison to other treatments with the technique.

Effect of pre-sowing treatments on germination and growth of French bean (Phaseolus vulgaris L.)

The experiment was laid out to study the effect of pre-sowing treatments with growth regulators viz. Indole acetic acid (IAA), Salicylic acid (SA) and Gibberellin acid (GA3) with different concentrations like 50 ppm and 100 ppm on physiological and growth attributes of French bean. The seeds treated with growth regulators for 8 hrs. And 12 hrs. Duration along with three replications of treatments. The experimental approaches like plot size 2.5×2.5 m, spacing 45×15 cm and ridge & furrow planting method used and transplanting followed. The significantly maximum germination percentage (95.95%) recorded with 100 ppm concentration of IAA in this seeds soaked for 12 hrs. (T6) which was better over control (87.17%). The maximum change in seed diameter (0.86 cm) was noticed in T6 i.e. seeds treated with IAA 100 ppm concentration and soaked for 12 hrs. And minimum recorded in control (0 cm). The better length of radical (9.03 cm) when seeds treated with 100 ppm concentration of GA3 and soaked for 12 hrs. (T4). The minimum days taken to emergence (5.11 days) occurred with T6 i.e. seeds treated with 100 ppm IAA and soaked for 12 hrs. Over control. The results revealed that significant change in growth attributes of French bean like maximum plant height (66.46 cm), number of branches per plant (7.85), minimum days taken to 50% flowering (31.15 days) recorded when seeds treated with 100 ppm GA3 and soaked for 12 hrs. (T4) over control or water treated seeds.

PENGARUH KONSENTRASI CUKA BAMBU DAN MACAM VARIETAS TERHADAP PERTUMBUHAN STEK LADA PERDU (Piper nigrum L.)

The purpose of this study is to determine the effect of concentration of petung bamboo vinegar, kinds of varieties, and the interaction of both on the growth of shrub pepper cuttings. The study used a factorial Randomized Complete Block Design (RCBD) with three blocks. The first factor is the concentration of petung bamboo vinegar (Dendrocalamus asper) consisting of 0 ppm, 1.000 ppm, 2.000 ppm, 3.000 ppm, 4.000 ppm, 5.000 ppm, and 1.000 ppm Indole Acetic Acid (IAA) as a synthetic growth regulator. The second factor is kinds of varieties: Natar-1 and Ciinten. The results showed that the use of petung bamboo vinegar for 5.000 ppm produces the highest number of leaves, fresh weight of shoots, and dry weight of shoots on Ciinten variety, while the Natar-1 variety with concentration of petung bamboo vinegar for 3.000 ppm obtained the highest fresh weight of shoots and dry weight of shoots. Ciinten variety obtained the highest results on shoot height, number of leaves, longest root length, number of roots, fresh weigh of shoots, dry weight of shoots, fresh weight of roots, and dry weight of roots. The interaction between the concentration of petung bamboo vinegar and kinds of varieties has no significant on all observed parameters. The use of petung bamboo vinegar at 3.000 ppm for Natar-1 and 5.000 ppm for Ciinten can replace the role of IAA growth regulator at concentrations of 1.000 ppm. Keywords: Dendrocalamus asper, bamboo vinegar concentration, Piper nigrum L., shrubs pepper cuttings, varieties.

Influence of Indole-3-Acetic Acid on Cellular Immune Responses of Galleria mellonella L. (Lepidoptera: Pyralidae) and Pimpla turionellae L. (Hymenoptera: Ichneumonidae) in a host-parasitoid system

The dietary effects of the well-known auxin member plant growth regulator, indole-3-acetic acid (IAA), on hemocyte counts and behavior of the model insect Galleria mellonella L. (Lepidoptera: Pyralidae) and its parasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) were examined. In vivo and in vitro experiments involved five treatments applied to larvae with doses of 50, 500, 1000, 5000, and 10000 ppm IAA and the controls. Total hemocyte counts increased at all applied IAA doses in G. mellonella whereas no effect was observed in P. turionellae. According to acridine orange-ethidium bromide double staining, none of the IAA doses affected the ratio of apoptotic and mitotic cells in vivo however a decrease in the number of late apoptotic cells at 1000 and 5000 ppm was observed at in vitro experiments in G. mellonella. An increase in the percentage of early apoptotic hemocytes at 1000 ppm and late apoptotic hemocytes at 500 ppm were determined in P. turionellae larvae. IAA also increased the ratio of mitotic hemocytes in the circulation of P. turionellae larvae at 50, 1000, and 10000 ppm. Changes in the encapsulation rate of Sephadex A-25 beads were not significant however the increase at 5000 ppm at 4th hour in non-melanized hemocytes was recorded as compared to the control. A strong decrease in melanization at 50 ppm and an increase at 50 and 1000 ppm in non-melanized bead count at 24 h were also observed. It can be concluded that IAA is inappropriate to use in combination with microbial control agents in IPM programs due to elevated hemocyte counts of the pest.

Indole-3-Acetic Acid induced oxidative stress in model host Galleria mellonella L. (Lepidoptera: Pyralidae) and its endoparasitoid Pimpla turionellae (L.) (Hymenoptera: Ichneumonidae)

Investigation of the antioxidant and oxidative effects of dietary indole-3-acetic acid (IAA), a plant growth regulator, on pest Galleria mellonella L. (Lepidoptera: Pyralidae) and its endoparasitoid Pimpla turionellae (L.) (Hymenoptera: Ichneumonidae) was aimed in this study. Different doses of dietary IAA (50-10,000 ppm) caused an increase in lipid peroxidation in the hemolymph of the host, G. mellonella (L.) and its endoparasitoid P. turionellae (L.). When compared to the control, higher doses of dietary IAA decreased CAT, SOD and GST enzymes’ activities in G. mellonella. At higher IAA doses, the activity of SOD enzyme in the hemolymph of P. turionellae significantly decreased while CAT enzyme activity showed no significant change when compared to the control. Additionally, GST activity in the endoparasitoid larval hemolymph significantly increased at 500 and 1000 ppm IAA doses. These findings indicate that incorporating IAA in the diet of model host G. mellonella larvae leads to oxidative stress and, also negatively affects the survivability of both the host and its endoparasitoid.

THE URBAN FARMING DENGAN HIDROPONIK MENGGUNAKAN ZAT PENGATUR TUMBUH UNTUK PENINGKATAN PERTUMBUHAN TANAMAN KANGKUNG

Urban farming activities with hydroponics techniques is one of the positive activities that can improve the creativity of people in urban areas. Urban farming activities can help improve the quality of people's lives, the environment, and play a role in maintaining food security. The study consisted of independent variables consisting of several groups, namely control (AB-Mix), 0.5 ppm of IAA (Indole Acetic Acid), BAP (Benzyl Adenine Purine) 0.5 and 1.0 ppm, and combination of IAA / BAP 0.5 /1.0 ppm. The purpose of this study was to study the effect of these treatments on water spinach plant yields that include leaves number, plant height, antioxidant content, and water content. The results of morphological growth showed that treatment with IAA 0.5 ppm was the most optimal while treatment with 1.0 ppm BAP had the average of the shortest plant height, the average number of leaves at least, and the shortest root length average. While the chlorophyll test showed that the system unit with the treatment of 0.5 ppm IAA had the highest total chlorophyll content with an average of 9.55 μg/ml. From the overall result, it can be assumed that the IAA of 0.5 ppm has the most positive effect on water spinach among other treatments.

Effect of Indole Acetic Acid and Boron on the Nutrient Contents of Summer Tomato Cultivar Bina Tomato-3

A pot experiment was carried out with tomato variety BINA Tomato-3 in the net house of the Department of Agricultural Chemistry, Bangladesh Agricultural University (BAU), Mymensingh from March to August, 2010 to study the effect of Indole Acetic Acid(IAA) and Boron(B) on the nutrient contents of BINA Tomato-3. There were four doses of IAA viz. 0, 20, 40 and 60 ppm and B viz. 0, 1.5, 2.0 and 2.5 kg ha-1. The experiment was laid out in a Completely Randomized Design (CRD) with 3 replications. The result revealed that nutrient contents of tomato fruits and plants such as N, P, Ca, Mg and B were significantly influenced by the application of IAA and B. The highest N (0.70%), Ca (0.69%), Mg (0.80%) in tomato fruits and highest N (0.40%), Ca (0.93%), Mg (0.90%) in tomato plants were obtained in 60 ppm IAA along with 2.0 kg B ha-1 and the lowest was obtained in the control treatment. In tomato fruits the highest P (0.41%) was found in IAA40B2.0 treatment, while the lowest P (0.18%) was recorded in IAA0B0.0. The lowest B (4.55µgg-1) was recorded in control, while the highest B (15.72µgg-1) was found in IAA20B2.0in tomato fruits. In tomato plants the highest P (0.34%) was recorded in IAA60B1.5 and the lowest P (0.20%) was found in control. The lowest B (4.15µgg-1) was recorded in IAA0B0.0, while the highest B(13.33µgg-1) was found in IAA40B2.0 in tomato plants. The overall results suggest that treatment IAA60B2.0 was the best from other treatments.

Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

Efficacy of IAA, IBA and magnesium nitrate on summer squash shelf life

A complete randomized design experiment with ten treatments and three replications was conducted to evaluate the effects of dipping fruits for 5 minutes in one of the following solutions: tap water, 10, 20 and 30 ppm of indoleacetic acid (IAA) or indolebeuteric acid (IBA) or 0.1, 0.2 and 0.3% magnesium nitrate salt (Mg(NO3)2) on the shelf-life characters of summer squash (Cucurbeta pepo L.) fruits, including that harvested incorrectly (without remaining part of the stem) as it proceeds by most Iraqi farmers. The results show a significant decrease in each of peroxidase enzyme activity to 4.94 absorbing unit/minute/ml and weight loss to 9.98% in the fruits that treated with 10 ppm of IAA compared to other treatments. Treating fruits with 20 ppm of IAA increased the fruits peel content of chlorophyll a and chlorophyll b. The fruits total soluble solids increased significantly to 5.33% when they dipped in 20 ppm IBA. There were non-significant differences between the treatments firmness, except the significant decrease to 5.14 kg/cm2 when 0.3% magnesium nitrate was used. Nevertheless, most differences between treatments were non-significant compared to the control fruits. Treating the fruits of summer squash with 10 ppm IAA was the best for decreasing peroxidase activity and fruits weight loss after keeping at ambient temperature (20-23°C and 40-55% relative humidity) for 5 days.

Facilitation of olive harvest by microbial indole acetic acid and an enzyme mixture

In this study, the effect of indole acetic acid (IAA) and/or a commercial enzyme mixture on the olive harvest was analyzed. IAA was produced by Gibberella fujikuroi. IAA (1500 and 3000 ppm) and/or the commercial enzyme preparation (1%) was mixed and applied to the olive trees before (3 weeks and 1 week) the harvest. The changes in protein, pectin and cellulose contents of fruits were determined monthly. While the cellulose and protein contents showed a decrease in 2 periods of 3 months (July-September and October-December), pectin contents increased in the same period. IAA and/or enzyme applications did not lead to any significant changes in the cellulose and protein contents of the fruit (p>0,001). However, the pectin amount in the trees where the enzyme application was performed before the harvest, showed a tendency to fall. The most convenient application with regard to the fruit and leaf fall was 3000 ppm IAA+1% enzyme application 3 weeks before harvest. While the fruit fall amount increased more when compared to the control and other groups with this application, the leaf fall decreased significantly.

Effects of Planting Date and Growth Hormone on Growth and Yield of Cauliflower

An experiment was conducted at the Horticulture Farm of Bangladesh Agricultural University, Mymensingh, during the period from October 2014 to March 2015 to study the effects of planting date and growth hormone on the growth and yield of cauliflower. The experiment consisted of two factors; Factor A: Three planting dates, such as P1: Planting on 1 November; P2: Planting on 15 November and P3: Planting on 1 December; Factor B: Four levels of Growth hormone, such as H0: No Hormone (control); H1: 10 ppm IAA (Indole-3 Acetic Acid); H2: 70 ppm GA3 (Gibberellic Acid) and H3: 10 ppm IAA + 70 ppm GA3. The experiment was laid out following Randomized Complete Block Design (RCBD) with three replications. In case of planting date, the highest plant height (63.26 cm), number of leaves per plant (24.13), leaf length (59.26 cm), leaf breadth (19.31 cm) at harvest, curd diameter (22.25 cm), marketable yield per hectare (28.11 t ha-1) were recorded from P2 and the lowest of those parameters were recorded from P3. In case of growth hormone the highest plant height (63.10 cm), number of leaves per plant (23.66), leaf length (59.05 cm), leaf breadth (18.98 cm) at harvest, curd diameter (22.39 cm), marketable yield per hectare (29.88 t ha-1) were recorded from H3 and the lowest of those perameters were recorded from H0. Combination of planting date and growth hormone the highest plant height (65.96 cm), number of leaves per plant (26.42), leaf length (63.64 cm), leaf breadth (20.92 cm) at harvest, curd diameter (25.75 cm), marketable yield per hectare (31.03 t ha-1) were recorded from planting on 15 November and 10 ppm IAA with 70 ppm GA3 (P2H3) and the lowest parameters (21.75 t ha-1) were recorded from planting on 1 December and no hormone (P3H0). It is apparent from the above results that the combination of planting on 15 November and 10 ppm IAA with 70 ppm GA3 (P2H3) was more productive from the other combinations.J. Environ. Sci. & Natural Resources, 9(2): 143-150 2016

Enhanced phenolic acids production in regenerated shoot cultures of Salvia virgata Jacq. after elicitation with Ag+ ions, methyl jasmonate and yeast extract

Phenolic acids, among the major secondary metabolites of Salvia species, are important bioactive phytochemicals, namely for their application in pharmaceutical industries. Biosynthesis of secondary metabolites, including phenolic acids in plant in vitro cultures is affected by biotic and abiotic elicitors, leading mostly to higher levels than in non-elicited ones. The present study was initially focused on achievement to a suitable procedure for direct multiple shoot regeneration on multinodal explants from 50days old seedlings of Salvia virgata Jacq. on Murashige and Skoog (MS) solid media supplemented with different concentrations of 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA). Then, the effects of different concentrations of Ag+ ions, yeast extract (YE) and methyl jasmonate (MeJA) on total phenolic and flavonoid contents, as well as some phenolic acids were studied in the regenerated shoots after 1,3 and 5days of elicitation in liquid free hormone MS medium. Based on the results, the maximum number of regenerated shoots (4.67) per responsive explant was obtained on MS medium containing 2ppm BAP and 0.05ppm IAA. As an effective abiotic elicitor, Ag+ ions could improve production of phenolic acids in the shoot cultures, while the highest content (26mg/g DW) of rosmarinic acid (RA) was reported on day 5 after exposure of regenerated shoots to 2.5ppm of Ag+ ions. Also, the highest contents of salvianolic acid A (Sal-A) (3.72mg/g DW) and caffeic acid (CA) (35.5mg/g DW) were found after elicitation of regenerated shoots with MeJA (11.2ppm) on day 3 and Ag+ ions (2.5ppm) on day 5, respectively. The results suggested that MeJA and Ag+ ions had the considerable ability to stimulate the production of valuable phenolic acids such as RA in the regenerated shoot cultures of S. virgata.

Effects of Indole-3-Acetic Acid on Germination in Lead Polluted Petri Dish of Citrullus lanatus (Thunberg) Matsumura and Nakai, Cucurbitaceae

Watermelon, Citrullus lanatus (Thunberg) Matsumura and Nakai is a tropical fruit vegetable. Indole-3-acetic acid (IAA) is a popular phytohormone while lead (Pb) is a common environmental pollutant in urban and sub-urban centers. C. lanatus were obtained from Benin City with a view to study the effects of IAA on their germination in Pb polluted environment. Germination percentage without IAA and Pb treatment in petri dish was significant after ten days. Hastened germination was observed when IAA and lead were used. About 100 % germination was recorded after seven days. This suggests that water melon seeds can initiate growth even in lead polluted environment. Optimum level of 5 ppm IAA with the different levels of lead treatments may be recommended. Most important was that higher concentrations of Pb in the control (without IAA) did not inhibit seedling shoot nor root growth. Longest seedling shoot length (cm) was 10.33 ± 1.24 and 12.13 ± 2.06 on the seventh and eighth day respectively with the combined treatment levels of 1 ppm IAA and 15 ppm Pb. On the ninth day, 15.27 ± 0.96 was obtained from 1 ppm IAA and 20 ppm Pb. Longest seedling root length (cm) values were recorded from the combined treatment levels of 0 ppm IAA and 10 ppm Pb for the seventh (9.10 ± 0.47) and ninth (10.37 ± 1.81) day respectively and 0 ppm and 15 ppm Pb on the eighth (9.37 ± 0.84) day. Significant means were also obtained with the treatment level of 0 and 20 ppm IAA. This present study suggest the germination of C. lanatus under Pb polluted environment may be rescued with optimum IAA.

Vegetative propagation of physic nut (Jatropha curcas L.) through stem cuttings

The selected healthy branches of Jatropha curcas were cut into 15 cm and 25 cm length having 4 to 5 nodes in each category of apical portion (thickness < 1cm), middle portion (1.0 to 1.5 cm) and basal portion (1.5 to 2 cm). The base positions of apical, middle, basal portions of cuttings were dipped in the 100, 200, 400, 800 and 1000 ppm of IAA (Indole-3-acetic acid) and IBA (Indole-3-butyric acid) respectively for four hours. After which the cuttings were planted in the polypots filled with rooting media consists of sand, soil and farm yard manure (FYM) in the ratio of 1:2:1. Maximum sprouting (100%) was observed in 25 cm as well as 15 cm length cuttings of different portions. In case of 25 cm length different sections like apical cuttings when treated with 200 ppm, 800 ppm IAA and 400 ppm IBA, basal cutting with 800 ppm IAA and 200, 400 ppm IBA and middle cuttings with 200 ppm IAA, similarly 15cm length basal portion cutting treated with 400 ppm IAA produce 100 per cent sprouting. Other characters like rooting percentage (93.33%), root length (37.66cm), fresh biomass (73.21g) and dry biomass (34.06g) were observed maximum in apical portion cutting of 25 cm length treated with 100 ppm IBA, 800 ppm IBA, 100 ppm IAA and 100 ppm IAA respectively where as root number (17.0) found maximum in middle portion cutting of 15cm length treated with 1000 ppm IAA. It showed that the apical portion of 25 cm length cuttings treated with IAA and IBA resulted in maximum sprouting, rooting percentage, root length, fresh biomass and dry biomass production.