Power plants are associated with numerous occupational health and safety risk factors, with psychosocial risks being particularly significant. This study examines work-life conflict and burnout among power plant employees and discusses the factors associated with these issues. This cross-sectional study focused on employees at three hydroelectric power plants in Turkey. The inclusion criteria included employees with at least one year of tenure. Using cluster sampling, three plants were selected in Adana, Ankara, and Samsun. The sample size was determined to be 262, and 201 employees participated, yielding a 76.7% response rate. Data were collected via face-to-face interviews using a structured questionnaire, which encompasses the sub-dimensions of a valid and reliable scale: The Work-Life Conflict and Burnout sub-dimensions of the Copenhagen Psychosocial Questionnaire-III (COPSOQ-III) were used to measure the dependent variables. The independent variables included age, education level, total and weekly working hours, perceived health status, and department. The dependent variables were work-life conflict and burnout. Ethical approval was obtained from the Gazi University Ethics Committee. Statistical analysis compared the Pearson chi-square test, Fisher's exact test, and Yates correction with a significance threshold of p < 0.05. The mean age was 40.83 years, with an average tenure of 11.54 years and a weekly work time of 43.51 hours. Most participants (94.5%) were male; technical unit workers comprised 71.6%. Health issues included smoking (39.8%) and chronic diseases (19.9%). Concerns about the working environment include insufficient knowledge about safety (25.4%) and lack of knowledge about risk assessments (32.3%). Many workers reported lacking personal protective equipment (11.4%) and rest areas (15.4%). Negative health impacts from work were noted by 31.8%. In addition, 51.2% believed that noise levels were outside the acceptable range. Two-thirds of employees reported inadequate measures against physical risks in the workplace. Many participants experienced work-life conflict (13.9%) and burnout (14.5%). High work-life conflict was significantly associated with younger age groups, less tenure, and negative perceived health status. Burnout was significantly related to the duration of employment, weekly working hours, and perceived health status. The study highlights the seriousness of burnout and work-life conflict among hydropower plant workers, emphasizing the need for administrative and organizational interventions to alleviate these issues. Regular occupational health and safety training, involvement in risk assessments, fair workload distribution, supportive work environments, and counseling services are recommended to reduce burnout and improve work-life balance.