Poultry Species Research Articles

Ochratoxin A in Poultry Supply Chain: Overview of Feed Occurrence, Carry-Over, and Pathognomonic Lesions in Target Organs to Promote Food Safety

Ochratoxin A (OTA) is a mycotoxin produced by fungi species belonging to the genera Aspergillus spp. and Penicillium spp. The proliferation of OTA-producing fungal species may occur due to inadequate practices during both the pre-harvest and post-harvest stages of feed. Consequently, poultry species may be exposed to high concentrations of this mycotoxin that can be transferred to animal tissues due to its carry-over, reaching dangerous concentrations in meat and meat products. Therefore, this review aims to propose a comprehensive overview of the effects of OTA on human health, along with data from global studies on the prevalence and concentrations of this mycotoxin in avian feeds, as well as in poultry meat, edible offal, and eggs. Moreover, the review examines significant gross and histopathological lesions in the kidneys and livers of poultry linked to OTA exposure. Finally, the key methods for OTA prevention and decontamination of feed are described.

Systematic Review and Meta-Analysis of Thermal Stress Assessment in Poultry Using Infrared Thermography in Specific Body Areas

Thermal stress is a health and welfare concern in the poultry industry. Poultry have specific thermoregulation strategies for heat stress (i.e., vasodilatation) or cold stress (i.e., vasoconstriction). Infrared thermal (IRT) analysis is a non-invasive temperature assessment technology with significant benefits compared to conventional temperature measurements, which are invasive and time-consuming. However, a wide range of IRT methodologies and equipment are used for temperature assessment in poultry. The aim of this study was to perform a systematic review and meta-analysis of IRT applications in poultry undergoing thermal stress. The bibliographic search yielded 17 records for qualitative synthesis and 10 for quantitative analysis. The results showed IRT is more commonly studied during heat stress than cold stress, and more research is being conducted on laying hens than other poultry species. Also, four body areas (parts of the head, body, face, and leg) were identified as common areas of interest for body surface temperature measurement. There is a clear thermoregulation response to thermal stress in poultry, with marked differences between featherless and feather-covered areas. IRT in poultry undergoing thermal stress has a good diagnostic value and represents an important welfare assessment tool for future research, particularly when combined with other welfare assessment methods.

Is the sex ratio of Japanese quail offspring equal?

Offspring sex ratios in avian species are of significant scientific interest, with implications for evolutionary biology and poultry production. This study investigated sex ratios in Japanese quail (Coturnix japonica), a valuable model for other poultry species due to its rapid generation interval. The study examined the impact of selection over generations, age at first egg (AFE), and body weight at AFE (BWAFE) on offspring sex ratios. The dataset included 4,282 Japanese quail records from 968 dams over eight generations, comprising two lines: one selected for high growth rate during 1-21 days of age and an unselected control line. Offspring sex ratio data were categorized based on dam characteristics: AFE (early: <48 days, medium: 48-52 days, late: >52 days) and BWAFE (low: <249g, medium: 249-268g, heavy: >268g). These categories represent below average, average, and above average values for each parameter, respectively. Analyses were done on pedigree and hatching records from two lines of selected and control quails. The chi square and logistic regression analyses exhibited insignificant associations between the examined predictor variables (generation, line, AFE, and BWAFE) and the sex ratio outcome in Japanese quail. Therefore, it can be concluded that the proportion of male and female offspring quail in the flock is statistically equal. However, regarding the BWAFE categories the residual analyses revealed a potential tendency toward a male-biased sex ratio within the medium category also, they suggest potential tendencies toward male-biased (eighth generation) and female-biased (sixth generation) sex ratios that warrant further investigation.

Exploration of Some Nutrient Absorption-Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan.

Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption-related genes could be used to study intestinal functionality and efficiency. This study aimed to evaluate the mRNA abundance of nutrient absorption-related genes and intestinal histology changes in laying Japanese quail during productive lifespan. A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n=45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi-IIb genes were compared among groups. Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi-IIb (p>0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p<0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p<0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p<0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p<0.05). In conclusion, although histology parameter changes were in-line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans.

Serological analysis in humans in Malaysian Borneo suggests prior exposure to H5 avian influenza near migratory shorebird habitats

Cases of H5 highly pathogenic avian influenzas (HPAI) are on the rise. Although mammalian spillover events are rare, H5N1 viruses have an estimated mortality rate in humans of 60%. No human cases of H5 infection have been reported in Malaysian Borneo, but HPAI has circulated in poultry and migratory avian species transiting through the region. Recent deforestation in coastal habitats in Malaysian Borneo may increase the proximity between humans and migratory birds. We hypothesise that higher rates of human-animal contact, caused by this habitat destruction, will increase the likelihood of potential zoonotic spillover events. In 2015, an environmentally stratified cross-sectional survey was conducted collecting geolocated questionnaire data in 10,100 individuals. A serological survey of these individuals reveals evidence of H5 neutralisation that persisted following depletion of seasonal H1/H3 HA binding antibodies from the plasma. The presence of these antibodies suggests that some individuals living near migratory sites may have been exposed to H5 HA. There is a spatial and environmental overlap between individuals displaying high H5 HA binding and the distribution of migratory birds. We have developed a novel surveillance approach including both spatial and serological data to detect potential spillover events, highlighting the urgent need to study cross-species pathogen transmission in migratory zones.

Genetic features of avian influenza (A/H5N8) clade 2.3.4.4b isolated from quail in Egypt

Several genotypes of the highly pathogenic avian influenza (HPAI) virus H5N8 subtype within clade 2.3.4.4b continue to circulate in different species of domestic birds across Egypt. It is believed that quail contribute to virus replication and adaptation to other gallinaceous poultry species and humans. This study provides genetic characterization of the full genome of HPAI H5N8 isolated from quail in Egypt. The virus was isolated from a commercial quail farm associated with respiratory signs. To characterize the genetic features of the detected virus, gene sequencing via Sanger technology and phylogenetic analysis were performed. The results revealed high nucleotide identity with the HPAI H5N8 virus from Egypt, which has multiple basic amino acid motifs PLREKRRKR/GLF at the hemagglutinin (HA) cleavage site. Phylogenetic analysis of the eight gene segments revealed that the quail isolate is grouped with HPAI H5N8 viruses of clade 2.3.4.4b and closely related to the most recent circulating H5N8 viruses in Egypt. Whole-genome characterization revealed amino acid preferences for avian receptors with few mutations, indicating their affinity for human-like receptors and increased virulence in mammals, such as S123P, S133A, T156A and A263T in the HA gene. In addition, the sequencing results revealed a lack of markers associated with influenza antiviral resistance in the neuraminidase and matrix-2 coding proteins. The results of the present study support the spread of HPAIV H5N8 to species other than chickens in Egypt. Therefore, continuous surveillance of AIV in different bird species in Egypt followed by full genomic characterization is needed for better virus control and prevention.

Safety and efficacy of a feed additive consisting of endo‐1,4‐β‐xylanase produced with Trichoderma reesei CBS 114044 (ECONASE® XT) for pigs for fattening, laying hens and minor poultry species (AB Enzymes Finland Oy)

Abstract Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of a product containing endo‐1,4‐β‐xylanase (ECONASE® XT) as a zootechnical feed additive (digestibility enhancer) for pigs for fattening, laying hens and minor poultry species. ECONASE® XT is available in two liquid and three solid forms and is produced with a genetically modified strain of Trichoderma reesei (CBS 114044). The FEEDAP Panel concluded that the genetic modification of the production strain does not raise any safety concerns, and viable cells of the production strain and its DNA were not detected in the final products. The Panel also concluded that ECONASE® XT is safe for pigs for fattening, laying hens and minor poultry species at the proposed conditions of use. The use of the additive raises no safety concerns for the consumers of products derived from animals fed with the additive or for the environment. The liquid and solid forms of ECONASE® XT are non‐irritant to the skin, but only the liquid forms were confirmed as non‐irritant to the eyes and not dermal sensitisers. The Panel could not conclude on the irritation potential to the eyes and the dermal sensitisation potential for the solid forms. Due to the proteinaceous nature of the active substance, the additive is considered to be a respiratory sensitiser. The additive has the potential to be efficacious in pigs for fattening at 20,000 BXU/kg complete feed, in all laying poultry species at 12,000 BXU/kg complete feed and in minor poultry species other than laying birds at 8000 BXU/kg complete feed.

Safety of a feed additive consisting of 3-phytase produced with Komagataella phaffii CECT 13171 (FSF10000/FLF1000) for poultry species, pigs for fattening and minor porcine species for fattening (Fertinagro Biotech S.L.).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety of the feed additive consisting of 3-phytase produced with a genetically modified strain of Komagataella phaffii (CECT 13171). The additive is intended to be used as a zootechnical additive for poultry species, pigs for fattening and minor porcine species for fattening. In a previous opinion, the EFSA Panel on Additives and Products or Substance used in Animal Feed (FEEDAP) Panel could not conclude on the taxonomic identification of the production strain. Moreover, the presence of viable cells of the production strain in the final formulations of the product could not be excluded. Therefore, no conclusions could be drawn on the safety for the target species, consumers, users and environment. In the present submission, the applicant provided supplementary information regarding the characterisation of the production strain and the absence of its viable cells in the final product. Based on the data provided, the identification of CECT 13171 as K. phaffii was confirmed and no viable cells of the production strain were detected in the final formulations of the additive. The FEEDAP Panel concluded that the 3-phytase produced with the genetically modified strain K. phaffii CECT 13171 does not give rise to any safety concern as regard to the production strain. Consequently, the additive, in both its liquid and solid formulations, is safe for the target species, consumers, users and environment.

Safety and efficacy of a feed additive consisting of an essential oil derived from the flowering tops of Thymbra capitata (L.) Cav. (Spanish type origanum oil) for use in all animal species (FEFANA asbl).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of an essential oil from the flowering tops of Thymbra capitata (L.) Cav. (Spanish type origanum oil) when used as a sensory additive in feed and in water for drinking for all animal species. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additive under assessment is considered safe up to the maximum use level in complete feed of 15 mg/kg for poultry species, 30 mg/kg for pigs and horses, 20 mg/kg for ruminants, 25 mg/kg for rabbits, dogs, cats and ornamental fish, and 125 mg/kg for salmonids. These conclusions were extrapolated to other physiologically related species. For any other species, the additive is safe at 15 mg/kg complete feed. The FEEDAP Panel considered that the use level in water for drinking is safe provided that the total daily intake of the additive does not exceed the daily amount that is considered safe when consumed via feed. The use of the additive in animal feed under the proposed conditions of use is safe for the consumer and the environment. Regarding user safety, the essential oil under assessment should be considered as an irritant to skin and eyes and as a dermal and respiratory sensitiser. Since T. capitata and its preparations were recognised to flavour food and its function in feed would be essentially the same as that in food, no further demonstration of efficacy was considered necessary.

Safety and efficacy of a feed additive consisting of Saccharomyces cerevisiae NCYC R618 (Benesacc®) for chickens for fattening and other poultry species for fattening and reared for laying (Global Nutritech Biyoteknoloji Ltd. Sti).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of Saccharomyces cerevisiae NCYC R618 as a zootechnical feed additive (functional group: gut flora stabiliser) for chickens for fattening and other poultry species for fattening and reared for laying. The additive has never been authorised. The strain under assessment S. cerevisiae (NCYC R618) qualifies for the qualified presumption of safety (QPS) and, consequently, the FEEDAP Panel concluded that the product is safe for the target species, the consumers and the environment. The FEEDAP Panel could not conclude on the skin and eye irritation or skin sensitisation potential of the additive. The additive should be considered a respiratory sensitiser. Due to the lack of sufficient data, the Panel cannot conclude on the efficacy of the additive under the proposed conditions of use.

Assessing awareness of IoT-driven strategies for poultry management in mitigating increased ambient heat in Enugu, Nigeria

The study examined awareness level of Internet of Things (IoT)-driven strategies in mitigating impacts of increased ambient heat load in poultry pens by poultry farmers in a smart city like Enugu, Nigeria. With the rising challenges of climate change, increased ambient heat load within poultry pens poses a significant threat to poultry production. Understanding farmers’ perception of impact of increased ambient heat load and their awareness of IoT-driven strategies for its mitigation is crucial for sustainable poultry system management in smart cities. Multi-stage random sampling technique was used to select 240 poultry farmers from the three Local Government Areas that make up Enugu City. A structured questionnaire was used to collect data from the respondents. Descriptive statistics and Likert rating scale were used to analyze the data. The study identified socio-demographic factors influencing farmers’ perception of impact of increased heat load and awareness/ adoption of IoT-driven mitigation strategies. Education level (45% secondary education), access to information (29.2%) and financial resources (22.9%) were key determinants. The findings revealed varying levels of awareness among poultry farmers regarding IoT-driven strategies while the constraints faced by farmers in implementing sustainable IoT-driven mitigation strategies included lack of awareness, lack of funding/high initial investment, limited access to technology, inadequate training, poor extension contact, water availability and climate variability. The study concluded that majority of poultry farmers shared similar perceptions about the impact of increased ambient heat load on poultry species but however were not aware of many of the IoT-driven strategies to mitigate the impact and were limited by a number of constraints or challenges to adopting IoT-driven mitigation strategies which altogether could improve poultry production system and help to achieve animal protein security in a smart city like Enugu, Nigeria. The study further recommended enlightenment of poultry farmers on the impact of increased ambient heat load on poultry species and available/sustainable/harmonized IoT-enabled guidelines or solutions to mitigate heat load in smart cities’ poultry production system.

In silico approximation to aflatoxin B1 metabolism and sensitivity in commercial poultry species based on empirical mathematical equations

Enzyme kinetic parameters for aflatoxin B1 metabolism have been reported for chicken, quail, turkey and duck, but an integrated in silico model has not been proposed. Both enzyme-catalyzed reactions and spontaneous reactions were modeled in the CellDesigner software and results were adjusted to Hill, Rational and Hoerl models. Results revealed that the higher amount of aflatoxin B1 epoxide produced in a short lapse of time and a low production of epoxide conjugated to glutathione explains the severe genotoxic effect of aflatoxin B1 in duck. Also, the higher amount of aflatoxicol produced is time-associated to aflatoxin B1 resistance in chicken. Finally, the cytotoxic effects in quail and duck are caused by a large aflatoxin B1 dialdehyde production in a short period of time.

Application of nutrigenomics and metagenomics in livestock

Nutrigenomics tools, such as transcriptomics referring to RNA, proteomics referring to protein molecules and metabolomics referring to metabolites, can be used to effectively explore the events at molecular level occurring in a genome that receives signals from various nutrients and responds to them through different metabolic processes in the living organism. Nutrigenomics has the potential to identify personalized diets, elucidate diet-related diseases, pinpoint genes associated with diet-gene interactions, and detect gene polymorphisms influenced by significant nutritional and environmental factors affecting genetic expression. Limited information is available on the dietary impact on gene expression associated with reproductive and productive traits in livestock. However, this might be feasible to initiate an understanding of the significance of the connection between specific nutrients or dietary ingredients and the gene expression regulation. Utilizing nutrigenomic approaches will augment researchers’ capacities to uphold animal health, optimize animal performance, and enhance the milk quality and meat quality. Despite being rapidly advancing science, nutrigenomics is still in its early phase. Metagenomics is emerging as a robust and efficient approach for the metagenome sequencing derived from environmental samples. Investigating the gut microflora of various livestock and poultry species holds the potential to identify probiotic communities possessing both immunity-boosting properties and growth-promoting. Analyzing the diversity of gut microbes’ aids in identifying strains that contribute to animals’ adaptability to their native conditions. This approach is optimal for unveiling the phylogenetic and evolutionary connections between contemporary species and the ancestors of livestock and poultry

Land Use Change and its Impact on Terrestrial Biodiversity of Surrounding Areas of Jim Corbett National Park

Located at the Shivalik Himalayas' foothills, near the worldwide popular hill-station of Nainital, the beautiful park is famous for being home to a huge number of Bengal tigers, the highest among any Indian national park. So many other mammals, including tigers, leopards and elephants (wild), roam in this park. On the edge of the Western Ramganga Reservoir, the Sonanadi zone is habitat to elephants and leopards, along with more than hundreds of species of fowl. Also CNP is a prime protected area of Uttarakhand forest as it provides ecological viable, profitable and sources of different income for local people. Dense forest cover is one among the most natural resources for every animal (Including human) directly and indirectly. But rapid excessive exploitations of the different vegetation, woods, shrubs species for human development here are now under the threat of biodiversity loss and uncovering forests. Present study focused on significance of terrestrial biodiversity, reason of land use changes and threats to terrestrial biodiversity (TB) because of land use change, terrestrial biodiversity of the local area is influencing by various ways. For continuous use of the forest ecosystems, it is very important to find the land use land covers changes and its negative impacts on biodiversity and surrounding environment. Forest resources are the full of vital material, base of direct and indirect human’s continuous progress and it needs to monitor the status and change of forest resources timely for logical exploitation of forests and its renewal. Present study focused on monitoring the changes in the forest areas, surrounding barren land, build-up area and natural resources.

Molecular and pathological screening of the current circulation of fowlpox and pigeon pox virus in backyard birds

Avian pox is a disease that has devastating impacts on both poultry and wild bird species. Avian pox is caused by various strains of avipoxviruses (APV). Nevertheless, the virus has been detected in pigeons and chickens that are raised in backyard areas, leading to substantial financial damage for small-scale producers. There is a lack of comprehensive information regarding the transmission of APV among birds in the backyards and residential areas. Hence, the present investigation closely monitored and observed APV in domesticated birds residing in backyard areas, with the aim of impeding the transmission of the virus to nearby poultry farms. In 2023, a total of fifty backyard flocks were surveyed for the presence of avian pox disease. Sixteen backyards (14 pigeons and 2 chickens) exhibited warty nodular lesions on their heads and nonfeathered body parts. APV was confirmed in nodular lesions by polymerase chain reaction (PCR) amplification and genetic sequencing. All samples from the lesions showed successful amplification of the p4b locus (core protein p4b). Four confirmed samples were tested for pathogenicity on the chicken embryo chorioallantoic membrane (CAM). Histopathological examination revealed ballooning degeneration and numerous intracytoplasmic inclusion bodies (Bollinger bodies) in the ectoderm of the infected CAM. Phylogenetic analysis revealed that the strains clustered into main clade A, with 11 in subclade A2 and 5 in subclade A1. Amino acid identity showed 100% similarity between the vaccine (fowlpox/VSVRI/Egypt) and some detected strains (PP537574 and PP537575). In addition, the PP537576.1 to PP537580.1 and PP537582.1 to PP537585.1 had 2-point mutations compared to the fowlpox/VSVRI/Egypt vaccine. The overall finding of low biosecurity levels in the investigated backyard birds emphasizes the significance of establishing sanitary measures and control vectors to reduce virus transmission routes and disease severity. In conclusion, it is necessary to emphasize the tracking of APV in backyard birds. Concurrently, we advised enhancing hygiene protocols, vector management, and subsequent vaccination to restrict the occurrence of APV outbreaks and prevent their transmission to neighboring poultry farms. Furthermore, it is crucial to incorporate molecular studies in order to enhance the vaccine seeds for disease management.

Safety Assessment on the Safety and Efficacy of an Additive of Chromium Propionate (KemTRACE Chromium) as a Feed Additive for All Growing Poultry Species (RP634)

An application was submitted to the Food Standards Agency in March 2021 from Kemin (‘the applicant’) for the new authorisation of an additive (KemTRACE Chromium) containing chromium propionate, under the category ‘zootechnical’ additive, functional group ‘other zootechnical additives’. The additive is proposed to be used at an inclusion rate of 0.2 – 0.4 mg/kg of complete feed (12% moisture) for all growing poultry. The Advisory Committee on Animal Feedingstuffs (ACAF) was asked to review the dossier and the supplementary information submitted by the Applicant, and to advise the Food Standards Agency and Food Standards Scotland (FSA/FSS) in evaluating the dossier. The FSA/FSS concluded, based on the ACAF’s advice, that the additive was correctly characterised, and that it is stable for 24 months at 25oC at 60% humidity, for 6 months as part of a premixture. Homogeneity in feed was also demonstrated. The mixture was pelleted at 90oC, but retention time was not given by the applicant, therefore no conclusion could be drawn on pelleting stability. No further concerns were raised for the identity section, Section II, of the dossier. The proposed level of inclusion in feed is safe for the target species, but no margin of safety could be determined. No conclusion could be drawn on the prenatal developmental toxicity potential of the additive, and therefore, on the safety of the additive for consumers. The additive is non-genotoxic, and no concerns about its metabolism were raised, however, no conclusion could be drawn on the applicant’s proposed 0-hour withdrawal period, given the impossibility to conclude on the prenatal developmental toxicity potential of the additive. The active substance is non-irritant to the skin, however another major ingredient, propionic acid, is corrosive to skin and eyes, and the additive should be considered as such. As the active ingredient is corrosive to skin and eyes and it may be sprayed onto the feed, it would be prudent to regard it as potentially harmful by inhalation. The additive is not a skin sensitizer. The additive poses an acceptable risk to the environment. Chromium propionate has the potential to be efficacious for all growing poultry at the maximum proposed dose of 0.4 mg/kg of complete feed with 12% moisture. The views of ACAF have been taken into account in this safety assessment which represents the opinion of the FSA/FSS.

A brief review on models for birds exposed to chemicals.

"A Who's Who of pesticides is therefore of concern to us all. If we are going to live so intimately with these chemicals eating and drinking them, taking them into the very marrow of our bones - we had better know something about their nature and their power."-Rachel Carson, Silent Spring. In her day, Rachel Carson was right: plant protection products (PPP), like all the other chemical substances that humans increasingly release into the environment without further precaution, are among our worst enemies today (Bruhl and Zaller, 2019; Naidu etal., 2021; Tang etal., 2021; Topping etal., 2020). All compartments of the biosphere, air, soil and water, are potential reservoirs within which all species that live there are impaired. Birds are particularly concerned: PPP are recognized as a factor in the decline of their abundance and diversity predominantly in agricultural landscapes. Due to the restrictions on vertebrates testing, in silico-based approaches are an ideal choice alternative given input data are available. This is where the problem lies as we will illustrate in this paper. We performed an extensive literature search covering a long period of time, a wide diversity of bird species, a large range of chemical substances, and as many model types as possible to encompass all our future need to improve environmental risk assessment of chemicals for birds. In the end, we show that poultry species exposed to pesticides are the most studied at the individual level with physiologically based toxicokinetic models. To go beyond, with more species, more chemical types, over several levels of biological organization, we show that observed data are crucially missing (Gilbert, 2011). As a consequence, improving existing models or developing new ones could be like climbing Everest if no additional data can be gathered, especially on chemical effects and toxicodynamic aspects.

Safety Assessment on the Safety and Efficacy of an Additive of Enterococcus faecium DSM 2121913, Bifidobacterium ⁠animalis spp. ⁠animalis DSM 16284 and Ligilactobacillus salivarius DSM 16351 (Biomin® C3) as a Feed Additive for Its Use in All Poultry Species for Fattening and Reared for Laying/ Breeding (RP1603)

An application was submitted to the Food Standards Agency in June 2022 from BIOMIN GmbH (‘the applicant’) for the renewal authorisation of an additive consisting of Enterococcus faecium DSM 21913, Bifidobacterium animalis ssp. animalis DSM 16284 and Ligilactobacillus salivarius DSM 16351 (Biomin® C3), under the category of ‘zootechnical additive’ and functional group ‘gut flora stabiliser’ for its use in chickens for fattening, chickens reared for laying and minor poultry species other than laying and for the extension of use of the additive under the same category for its use in all poultry species for fattening and reared for laying/breeding. The additive is proposed to be used at a minimum inclusion rate of 1 × 108 CFU/kg in complete feed or 5 × 107 CFU/L in drinking water. The FEEDAP Panel concluded that Biomin® C3 remains safe for chickens for fattening, chickens reared for laying, and minor poultry species other than laying, as well as for consumers and the environment, under the authorized conditions of use. The Panel concluded that extending the use of the additive to all poultry for fattening and reared for laying/breeding is safe for the target species, consumers, and the environment. The additive is efficacious at a minimum inclusion level of 1 × 108 CFU/kg in complete feed and 5 × 107 CFU/L in water. The additive should be considered as a potential respiratory sensitiser but as non-irritant to eyes and skin. Conclusions could not be drawn on the potential to cause skin sensitisation. FSA/FSS has reviewed the applicant’s renewal and extension of use application, supporting documentation, and other regulators risk assessments, most notably the EFSA risk assessment opinion and consider sufficient evidence has been demonstrated to conclude without further questions or risk assessment. This document represents the opinion of the FSA and FSS.

Creating a novel method for chicken primordial germ cell health monitoring using the fluorescent ubiquitination-based cell cycle indicator reporter system

The most current in vitro genetic methods, including gene preservation, gene editing and developmental modelling, require a significant number of healthy cells. In poultry species, primordial germ cells (PGCs) are great candidates for all the above-mentioned purposes, given their easy culturing and well-established freezing method for chicken. However, the constant monitoring of cultures can be financially challenging and consumes large amounts of solutions and accessories. This study aimed to introduce the Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) complex into the chicken PGCs. FUCCI is a powerful transgenic tool based on the periodic protein expression changes during the cell cycle. It includes chromatin licensing and DNA replication factor 1 attached monomeric Kusabira-Orange and Geminin-attached monomeric Azami-Green fluorescent proteins, that cause the cells to express a red signal in the G1 phase and a green signal in S and G2 phases. Modification of the chicken PGCs was done via electroporation and deemed to be successful according to confocal microscopy, DNA sequencing and timelapse video analysis. Stable clone cell lines were established, cryopreserved, and injected into recipient embryos to prove the integrational competency. The cell health monitoring was tested with medium change experiments, that proved the intended reactions of the FUCCI transgene. These results established the future for FUCCI experiments in chicken, including heat treatment and toxin treatment.

Histopathological and Molecular Identification and Characterization of Pneumonic Pasteurellosis in Sheep and Goats from Outbreaks at AS-Sharika Province, Egypt

Pneumonic Pasteurellosis highly fatal septicemic disease in multi-animal species in bovine, ovine, Poultry and swine. The strain identified from ovine pneumonia were characterized by MLST (Multi-host and RIRDC databases) and virulence-associated gene (VAG) typing and compared with bovine isolates. Recording outbreak of pasteurellosis with high mortality &amp; History of several deaths in different farm and homeowner cases of sheep and goats with severe respiratory illness after shipping them from long distance Elarish and Sinai in period to El-Sharqia province. Most cases that still live are treated with antibiotics after make sensitivity test on sampling from sick cases and most cases recovered and responded to treatments. Histopathological changes of lung tissue from recently dead cases showed the proliferation of pasterulla multicide in the respiratory system. The presence of Pasteurella multocida in swap samples collected from per acute &amp; acute cases of sheep &amp; goats was detected by bacteriological examination and culture.