Grapevine red blotch is a relatively new disease of grapevines that was first described on ‘Cabernet Sauvignon’ at the Oakville, CA, Research Field Station in 2008. A geminivirus, grapevine red blotch virus (GRBV, genus Grablovirus, family Geminiviridae), has consistently been isolated from symptomatic grapevines (Al Rwahnih et al. 2013), and Koch’s postulates have recently been fulfilled, proving that GRBV is the causal agent of red blotch disease (Yepes et al. 2018). Since its initial characterization, GRBV has been found throughout the United States (Krenz et al. 2014), as well as in germplasm repositories (Al Rwahnih et al. 2015; Thompson et al. 2018). However, the incidence of GRBV in hybrid grapes grown in the U.S. Midwest is not known. In 2016, potted rooted cuttings of a Vitis sp. hybrid ‘Crimson Cabernet’ (Cabernet Sauvignon crossed with ‘Norton’) near Union, MO, displayed typical symptoms of GRBV. Three potted vines were screened by polymerase chain reaction (PCR) for GRBV by Agdia Testing Services (Elkhart, IN), and all three tested positive for GRBV. In July 2017, leaf tissue was collected from eight asymptomatic Crimson Cabernet grapevines from a vineyard located near Hermann, MO. Total nucleic acids were isolated from each of the samples, and a DNA segment of 231 bp corresponding to the predicted coat protein region of GRBV was amplified by PCR from two of the eight samples. The PCR primers were CP-Forward (5′-AGCGGAAGCATGATTGAGACATTGACG-3′, nucleotide positions 1,073 to 1,099) and CP-Reverse (5′-AACGTATGTCCACTTGCAGAAGCCGC-3′, nucleotide positions 1,329 to 1,304). In a follow-up visit to the vineyard in August, we detected GRBV in two out of 10 samples with the set of primers for the CP gene and a second set of primers for the replicase gene, as described in Krenz et al. (2014). In a subsequent visit in late October, we observed red blotch symptoms in many of the vines in this vineyard. An additional 10 samples were collected during the late October visit for PCR analysis: eight samples from symptomatic vines and two from asymptomatic vines of the Crimson Cabernet hybrid cultivar. Seven of the eight symptomatic vines were positive, and one of the asymptomatic vines was also positive for GRBV. The entire GRBV genome sequence was determined for one sample (MO-CC7) and compared with other GRBV sequences by a Basic Local Alignment Search Tool (BLAST). The genome of MO-CC7 (GenBank accession no. MH732736) has a size of 3,205 nucleotides and is similar to GRBV isolates ONRB4 (GenBank accession no. KY316022.1) and 93-26 (GenBank accession no. KY426922), with sequence identities of 99%. A comprehensive survey has been planned to examine the incidence of GRBV in the commonly grown hybrid grapes in Missouri.