Potential Probe Research Articles

A REVIEW ON BENZANTHRONE LUMINOPHORES FOR RAPID AND HIGH-RESOLUTION IMAGING OF PARASITES BY CONFOCAL LASER SCANNING MICROSCOPY

Nowadays, is growing interest in investigation of parasites and their anatomic structure. Most common tools are linked with usage of luminophores and luminescent microscopy techniques. In recent years, a variety of fluorescent probes have been developed and widely used to realize the visualization of certain structures [1]. The benzanthrone compounds captivated a lot of interest as fluorescent probes for biomedical technologies because of remarkable spectral properties and negligible fluorescence in the aqueous phase [2]. Notably, the spectral characteristics of benzanthrone dyes meet the criteria for an ideal bio-imaging agent, featuring a high extinction coefficient, bright fluorescence, photo-, thermo- and chemical stability, and reduced background signal [3]. Fluorescent molecular dyes, currently used to study cell membranes, make lipid structures visible through optical techniques [4] and act as potential fluorescent probes for biomolecules [5]. There are studies in the literature where the benzanthron phosphors have confirmed their application for visualization of biological objects [6-8]. Despite high activity in this field, still it`s huge request for specific fluorescent probes for biological objects. The confocal laser scanning microscope (CLSM) is a powerful tool for providing high-resolution optical sections. CLSM can be used to analyse images of morphological structures and to place organ or organ systems of interest in their anatomical context. Luminescence imaging techniques are increasingly utilized for exploring the structure and properties of biological objects. Laser-induced fluorescence stands out as a sensitive method, capable of detecting even a single-molecule under specific conditions [9], which makes it a powerful bioanalytical tool for life sciences [10 12]. Benzanthrone derivates are used for CLSM imaging as fluorescence probe for various biological objects because benzanthrones render the specimen fluorescent to examine the stained samples by optical sectioning [13,14]. This review provides inputs in utilisation of different benzanthrone luminophores for examination of the parasites.

Multiple potential drop measurement – A new method for crack detection and crack length measurement

The Direct Current Potential Drop Method (DCPDM) is widely used for crack detection and crack length measurement in fatigue experiments. Unfortunately, crack length measurements are only possible when a calibration for a specified crack initiation site and potential probe location is available. Investigations by Hartweg and Bär (2019) as well as Nahbein and Bär (2022) on notched round specimens equipped with three potential probes placed in a distance of 120° on the circumference have shown that the crack initiation site can be determined using a simple vector-based model. In this model the normal vector of a plane span by the three potentials is calculated in cylindrical coordinates. In the present work a more detailed investigation of crack initiation and propagation is undertaken to show the possibilities offered by this method. During fatigue tests with the simultaneous measurement of the three potential drops using amplifiers of the control electronics, overloads were introduced in fixed intervals to mark the crack front on the fracture surface. The measurement of both crack depth and fractured area allows a calibration of the potential drop measurement. The calibration was performed on the radius coordinate n of the normal vector using a function suggested by Tiedemann (2016). The multiple potential drop measurement enables an early detection of crack initiation as well as a location of the crack initiation site on the circumference of the specimen. Using the Tiedemann function fitted to the measured data, it is possible to determine the crack length independent of the crack initiation site on the circumference of the specimen.

Design, synthesis, and biological evaluation of N-(pyridin-3-yl)pyrimidin-4-amine analogues as novel cyclin-dependent kinase 2 inhibitors for cancer therapy

The discovery and development of CDK2 inhibitors has currently been validated as a hot topic in cancer therapy. Herein, a series of novel N-(pyridin-3-yl)pyrimidin-4-amine derivatives were designed and synthesized as potent CDK2 inhibitors. Among them, the most promising compound 7l presented a broad antiproliferative efficacy toward diverse cancer cells MV4-11, HT-29, MCF-7, and HeLa with IC50 values of 0.83, 2.12, 3.12, and 8.61 μM, respectively, which were comparable to that of Palbociclib and AZD5438. Interestingly, these compounds were less toxic on normal embryonic kidney cells HEK293 with high selectivity index. Further mechanistic studies indicated 7l caused cell cycle arrest and apoptosis on HeLa cells in a concentration-dependent manner. Moreover, 7l manifested potent and similar CDK2/cyclin A2 nhibitory activity to AZD5438 with an IC50 of 64.42 nM. These findings revealed that 7l could serve as ahighly promisingscaffoldfor CDK2 inhibitors as potential anticancer agents and functional probes.

Excited-State Dynamics of a Photoacid: A Potential Probe for Recognizing Transition from Lamellar to Nonlamellar Inverted Structures of Liposome based Nanocarriers.

Liposomes of a cationic lipid dioctadecyldimethylammonium bromide (DODAB) are efficient nanocarriers of nucleic acids. Incorporation of a neutral lipid monoolein (MO) in excess (xMO >0.5) changes the lamellar organization of DODAB liposomes into non-lamellar inverted structures of DODAB/MO liposomes facilitating nucleic acid delivery to cells. Photoexcitation of 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS), a photoacid, initiates an excited state proton transfer (ESPT) reaction in its protonated form (ROH*) generating the deprotonated anionic form (RO- *). The fluorescence intensity ratio (IROH* /IRO-* ) of these two forms is governed by the ESPT dynamics, and increases with increasing MO content (xMO ) in the cationic liposomes of DODAB. Transition from lamellar organization of DODAB liposomes into non-lamellar inverted structures of DODAB/MO liposomes, due to incorporation of MO (xMO ~0.7), is manifested by a significant increase of ESPT time (τPT ) and the time constant of wobbling motion (τW ) of HPTS. Thus, the lamellar organizations of DODAB or DODAB-rich (xMO 0.2) liposomes and the non-lamellar organizations of MO-rich (xMO ~0.7) liposomes are recognized by significantly different excited state dynamics of the photoacid.

Iron isotope evidence in continental intraplate basalts for mantle lithosphere imprint on heterogenous asthenospheric melts

Iron isotope studies on ocean island basalts (OIBs) and mid-ocean ridge basalts (MORBs) have disclosed the contribution of pyroxenite lithologies in the generation of basaltic magmas. Whether Fe isotopic compositions of continental intraplate basalts can be applied to trace lithological heterogeneity within the mantle source regions remains poorly investigated. To explore the systematics of stable Fe isotopes as a potential probe of lithological heterogeneity in the source of continental intraplate basalts, we present twenty-four Fe isotope data on a suite of well-characterized Cenozoic basalts from SE China. The samples show a large range of Fe isotope values (δ56Fe = +0.09‰ to +0.20‰), which correlate with SiO2, CaO/Al2O3, Ti/Eu, Hf/Hf*, Zr/Nb, Dy/Yb, La/Yb, Nb/Y, K/La, Sr/Ce, δ66Zn and estimated equilibrium pressures. The samples with the highest δ56Fe values represent early-stage low-silica basalts with moderately enriched Sr-Nd isotope ratios and high δ66Zn values, while the late-stage high-silica basalts display a broad δ56Fe decrease with increasing 87Sr/86Sr and with decreasing εNd and δ66Zn. We demonstrate that the heaviest Fe isotope signatures cannot be derived from a pure peridotite source and require a pyroxenite component in the source. Combined with other geochemical proxies, we show that the heaviest basalt compositions are consistent with low-degree melts produced by the adiabatic decompression of a carbonated pyroxenite-bearing asthenospheric mantle. Mixing of these hybrid melts with melt produced from in-situ melting of the subduction-modified sub-continental lithospheric mantle (SCLM) reproduces the Fe-isotope variability of the late-stage basalts. Our results demonstrate the significance of lithological heterogeneity in the mantle source of continental intraplate basalts and highlight the subsequent imprint of mantle lithosphere in the evolution of their Fe isotope compositions. Finally, this study exemplifies the potential of the Fe-Zn stable isotope pair for mantle geochemistry; coupled with traditional radiogenic isotopes and major and trace element concentrations, they formed an efficient tool to trace the nature and contribution of the mantle source components in the formation of intraplate basalts.

Recent Progress in Transition Metal Dichalcogenides for Electrochemical Biomolecular Detection.

Advances in the field of nanobiotechnology are largely due to discoveries in the field of materials. Recent developments in the field of electrochemical biosensors based on transition metal nanomaterials as transducer elements have been beneficial as they possess various functionalities that increase surface area and provide well-defined active sites to accommodate elements for rapid detection of biomolecules. In recent years, transition metal dichalcogenides (TMDs) have become the focus of interest in various applications due to their considerable physical, chemical, electronic, and optical properties. It is worth noting that their unique properties can be modulated by defect engineering and morphology control. The resulting multifunctional TMD surfaces have been explored as potential capture probes for the rapid and selective detection of biomolecules. In this review, our primary focus is to delve into the synthesis, properties, design, and development of electrochemical biosensors that are based on transition metal dichalcogenides (TMDs) for the detection of biomolecules. We aim to explore the potential of TMD-based electrochemical biosensors, identify the challenges that need to be overcome, and highlight the opportunities for further future development.

Alendronate-derived carbon dots for bioimaging and effective osteoblastic differentiation induction and promotion

Carbon dots (CDs) belong to zero-dimensional carbon-based nanomaterials, which are promising in various biomedical applications including imaging, biosensing and drug delivery. In this work, we have synthesized novel fluorescent CDs (Alen-CDs) derived from alendronate, an anti-osteoporosis drug, through a one-step hydrothermal method. The ability of Alen-CDs for fluorescent imaging was evaluated via in vitro cellular bioimaging. In addition, the capability of Alen-CDs to induce in vitro osteoblastic differentiation was investigated by alkaline phosphatase staining, activity test and alizarin red staining. As demonstrated, Alen-CDs was effective for in vitro fluorescent imaging without obvious toxicity. Also, Alen-CDs promoted the survival of rat bone marrow mesenchymal stem cells and stimulated osteoblastic differentiation. In conclusion, Alen-CDs are a new type of nanomaterial with good biocompatibility and fluorescent properties. Alen-CDs could be applied as a potential probe for bioimaging and a promising nanodrug for bone regeneration as well as the combination to be used as a theranostic agent.

Di-berberine conjugates as chemical probes of Pseudomonas aeruginosa MexXY-OprM efflux function and inhibition

The Resistance-Nodulation-Division (RND) efflux pump superfamily is pervasive among Gram-negative pathogens and contributes extensively to clinical antibiotic resistance. The opportunistic pathogen Pseudomonas aeruginosa contains 12 RND-type efflux systems, with four contributing to resistance including MexXY-OprM which is uniquely able to export aminoglycosides. At the site of initial substrate recognition, small molecule probes of the inner membrane transporter (e.g., MexY) have potential as important functional tools to understand substrate selectivity and a foundation for developing adjuvant efflux pump inhibitors (EPIs). Here, we optimized the scaffold of berberine, a known but weak MexY EPI, using an in-silico high-throughput screen to identify di-berberine conjugates with enhanced synergistic action with aminoglycosides. Further, docking and molecular dynamics simulations of di-berberine conjugates reveal unique contact residues and thus sensitivities of MexY from distinct P. aeruginosa strains. This work thereby reveals di-berberine conjugates to be useful probes of MexY transporter function and potential leads for EPI development.

Fortuitous Observations of Potential Stellar Relay Probe Positions with GBT

Recent theoretical and observational works have investigated the possibility that extraterrestrial intelligence could use the Sun as a gravitational lens in order to aid communication across interstellar distances. Unlike other targeted SETI searches where the drift rate of any artificial extraterrestrial signals may be unknown up to some large upper limit, the drift rates of any solar system relay probes would be known and set only by the motion of the Earth. One recent work used purpose-designed Green Bank Telescope (GBT) observations to search for signals from a hypothetical communications probe several hundred astronomical unit from the Sun at the antipode of the α Centauri AB system. To further aid in the advancement of relay-probe searches, we present a table of 1764 archival GBT observations which fortuitously fall near the positions of hypothetical probes communicating with stars within 100 pc and compute the drift rates for these probes.

Impact of the Galactic bar on tidal streams within the Galactic disc

Tidal streams of disrupted clusters are powerful probes of the gravitational potential of the Galaxy and they are routinely detected in the stellar halo of the Milky Way. It was recently shown that tidal streams of open clusters can now also be detected within the Milky Way disc. In this work, we highlight the fact that disc tidal streams also provide a powerful new diagnostic of the non-axisymmetric disc potential and may, in principle, provide a new constraint on the pattern speed of the Galactic bar. In particular, we show how the stream-orbit misalignment for an open cluster on a quasi-circular disk orbit in the solar vicinity varies as a function of the position with respect to the bar resonances. The angular shift rises beyond corotation, reaching values as high as 30° close to the outer Lindblad resonance (OLR), then dropping again and reversing its sign beyond the OLR. We applied this mechanism to the recently detected tidal stream of the Hyades open cluster and we note that the detected stream stars would be very similar when taking a potential a priori with no bar or with a fast pattern speed of 55 km s−1 kpc−1 (or lower than 30 km s−1 kpc−1). However, we find that candidate stream stars are different than previously detected ones when adopting a potential with a bar pattern speed of 39 km s−1 kpc−1, which is consistent with the most recent determinations of the actual Galactic bar pattern speed. Previously detected Hyades candidate members would, on the other hand, favour a barless galaxy or a fast bar of pattern speed 55 km s−1 kpc−1. Interestingly, the previously reported asymmetry in star counts within the leading and trailing tails of the Hyades tidal stream persists in all cases. Our study conclusively demonstrates that the effect of disc non-axisymmetries cannot be neglected when searching for tidal streams of open clusters and that current candidate members of the Hyades stream should not be trusted beyond a distance of 200 pc from the cluster. Moreover, our study allows for ideal targets to be provided for high-resolution spectroscopy follow-ups, which will enable conclusive identifications of the Hyades stream track and provide novel independent constraints on the bar pattern speed in the Milky Way.

Perception about human papillomavirus vaccination among middle adolescent school girls in Addis Ababa, Ethiopia 2023: qualitative study

BackgroundHuman papillomavirus vaccine uptake among school girls in Ethiopia was still low and uptake was majorly related to perception regarding the vaccine. This study explored school girls’ perceptions of Human papillomavirus vaccination in Addis Ababa, Ethiopia.ObjectivesThe main aim of this study was to explore the perception of secondary school girls towards Human Papilloma Virus vaccine in Addis Ababa, Ethiopia 2023.MethodsA qualitative research using focused group discussions were used to explore middle adolescent school girls’ perceptions of Human papillomavirus in Addis Ababa Ethiopia from March 2023-April 2023. A focus group discussion guide was employed with potential probes to achieve study objectives. A convenience sampling technique was performed to select study participants. The collected data were transcribed and translated into English and thematic analysis was done by using Atlas-Ti software version 7.5.16.ResultTwo dominant themes emerged from the study; perceived benefits and potential barriers to Human papillomavirus vaccine uptake. Poor awareness, lack of family support, perception of poor vaccine quality, fear of side effects, misconceptions, and myths are potential barriers to the Human papillomavirus vaccine. Some students perceive the vaccine as important in the prevention of cervical cancer, others are not sure about the importance of the vaccine and many students think that the vaccine can cause infertility and other beliefs it can cause diseases.ConclusionThe perceived benefits of the Human papillomavirus vaccine was; it prevents cervical cancer enhance acceptability and can be an important motivation for Human papillomavirus vaccination among students. The main barriers to vaccine uptake and acceptance were; lack of awareness, misconceptions, and myths, lack of credible information sources of vaccines, perception of poor vaccine quality, Poor family support, and cultural and religious perspectives. We recommend the development of strong collaborations that work on identified challenges.

Molecular design and synthesis of methoxy-substitued spiropyrans with photomodulated NIR-fluorescence.

This study focuses on the molecular design and synthesis of salt spiropyrans with near-IR fluorescence. The structure of the obtained compounds was confirmed by NMR, IR and mass spectroscopy. In the course of studying the spectral and photoluminescent characteristics, it was possible to reveal the effect of some substituents in various positions on the properties of spiropyran dyes. Due to the structural similarity of one of the isomers to cyanine dyes, the obtained compounds are of interest as potential fluorescent probes for bioimagimg, in particular, for DNA studies. To reveal their ability of binding to DNA molecules molecular docking was carried out. Toxic effects of compounds demonstrating NIR fluorescence were studied on biofilms, as well as using bacterial lux-biosensors.

Two Polarity-Sensitive Fluorescent Probes Based on Curcumin Analogs for Visualizing Polarity Changes in Lipid Droplets.

As a class of highly dynamic organelles, lipid droplets (LDs) are involved in numerous physiological functions, and the changes in polarity of LDs are closely related to a variety of diseases. In this work, we developed two polarity-sensitive fluorescent probes (CC-CH and CC-Cl) based on curcumin analogs. CC-CH and CC-Cl with a donor-acceptor-donor (D-A-D) structure exhibited the property of intramolecular charge transfer (ICT); thus, their fluorescence emissions were significantly attenuated with increasing ambient polarity. Cell experiments indicated that CC-CH and CC-Cl showed excellent photostability, a low cytotoxicity, and a superior targeting ability regarding LDs. After treatment with oleic acid (OA) and methyl-β-cyclodextrin (M-β-CD), the polarity changes of LDs in living cells could be visualized by using CC-CH and CC-Cl. In addition, CC-CH and CC-Cl could monitor polarity changes of LDs in different pathological processes, including inflammatory responses, nutrient deprivation, and H2O2-induced oxidative stress. Therefore, CC-CH and CC-Cl are promising potential fluorescent probes for tracking intracellular LD polarity changes.

Phase slip from weak links formed in artificially-stacked NbSe2

The rich nature of van der Waals interactions between artificially-stacked atomic layers has been demonstrated by various quantum states and resonant tunneling transport in low-dimensional materials. However, the role of topological fluctuations in quantum transport through artificially-stacked junctions of 2D superconducting materials, and the resulting energy dissipation, remain elusive. In this research, unique phase-slip centers are designed in artificially-stacked junction areas, where nonequilibrium quasiparticles are formed and relaxed with energy dissipation. The phase slips are observed as voltage steps (peaks or valleys) in transport measurements across a junction between two exfoliated NbSe2 flakes, and at a distance of 4 μm from the junction using local and nonlocal chemical potential probes. Accordingly, two types of energy dissipation modes are newly identified in the artificially-stacked NbSe2 when subjected to an in-plane magnetic field, which implies distinct vortex formation and current flow in the superconducting junction under magnetic fields.

Two unique M-MOFs (M:Zn, Mn) based on various metal-oxygen chains for good sensitivity sensing and magnetic properties

In this paper, two novel two-dimensional MOFs were synthesized by solvothermal reaction of 2,4-di(2′,5′-dicarboxylphenyl)benzoic acid ligand and Zn(Ⅱ) and Mn(Ⅱ) metal ions: {[Zn2(HL)(H2O)(DMF)2]·(H2O)·(DMF)}n (1), {[Mn2(HL)(H2O)(DMF)2]·(H2O)·(DMF)}n (2). Complexes 1 and 2 are unique two-dimensional skeletons with particular metal-oxygen chains in their structures. The solid-state luminescence spectrum of complex 1 shows that its maximum emission peak is 448 nm. Further fluorescence studies of complex 1 show that it can efficiently detect Fe3+ and Cr2O72− in aqueous solution with a detection limit of 4.1 × 10−4 M and 2.7 × 10−4 M respectively, and has good recycling performance, so it is a potential bifunctional fluorescent probe material. The mechanism of fluorescence sensing quenching was also investigated in detail. Furthermore, magnetic studies have shown that there is an antiferromagnetic interaction between Mn(II) ions of complex 2.

Hydrogel Micropillar Array for Temperature Sensing in Fluid.

Localized temperature sensing and control on a micron-scale have diverse applications in biological systems. We present a micron-sized hydrogel pillar array as potential temperature probes and actuators by exploiting sensitive temperature dependence of their volume change. Soft lithography-based molding processes were presented to fabricate poly N-isopropyl acrylamide (p-NIPAAm)-based hydrogel pillar array on a glass substrate. Au nanorods as light-induced heating elements were embedded within the hydrogel pillars, and near-infrared (NIR) light was used to modulate temperature in a local area. First, static responses of the micro-pillar array were characterized as a function of its temperature. It was shown that the hydrogel had a sensitive volume transition near its low critical solution temperature (LCST). Furthermore, we showed that LCST could be readily adjusted by utilizing copolymerizing with acrylamide (AAM). To demonstrate the feasibility of spatiotemporal temperature mapping and modulation using the presented pillar array, pulsed NIR light was illuminated on a local area of the hydrogel pillar array, and its responses were recorded. Dynamic temperature change in water was mapped based on the abrupt volume change characteristics of the hydrogel pillar, and its potential actuation using NIR light was successfully demonstrated. Considering that the structure can be arrayed in a two-dimensional pixel format with high spatial resolution and high sensitive temperature characteristics, the presented method and the device structure can have diverse applications to change and sense local temperatures in liquid. This is particularly useful in biological systems, where their physiological temperature can be modulated and mapped with high spatial resolution.

Recent ALICE results on quarkonium production in nuclear collisions

Quarkonium production has long been regarded as a potential probe of deconfinement in nucleus-nucleus collisions. Recently, the production of J/ψ via regeneration within the quark-gluon plasma (QGP) or at the phase boundary has been identified as an important ingredient for the interpretation of quarkonium production results from lead-lead collisions at the Large Hadron Collider (LHC). Quarkonium polarization could also be used to investigate the properties of the hot and dense medium created at LHC energies, as well as the initial stages of the heavy-ion collision. In this contribution, the latest ALICE quarkonium results are presented and discussed. These include, among others, the nuclear modification of (prompt, non-prompt and inclusive) J/ψ, the ψ(2S) production, and the J/ψ polarization, all measured in lead-lead collisions at the LHC. The results are compared with available theoretical model calculations.

From Ideal to Stack-like Contacting of an SOC

The cell performance of a solid oxide cell strongly depends on the testing environment and conditions. In case of an inert testing set-up with ceramic (Al2O3) flow fields and gold (cathode) and nickel (anode) contact grids the losses inside the cell determine the performance [1]. For an electrolyte-supported cell with a nickel / gadolinium-doped ceria (Ni/CGO) fuel electrode and LSCF air electrode, the ideal cell behavior has been characterized by electrochemical impedance spectroscopy (EIS) and the subsequent analysis of the distribution of relaxation times (DRT) considering technically meaningful operating conditions. Furthermore, the deconvolution and quantification of the gas diffusion polarization has been shown with the help of ternary fuel gas mixtures with different gas diffusion coefficients. [2-4]In this contribution, the electrochemical characterization is now extended to a stack-like contacting of the solid oxide cell with different metallic interconnectors, protective coatings and contact layers. The resulting contact loss is quantified with a method by Kornely et al. [5] using potential probes at the interconnector and the air electrode in order to enable voltage loss measurements. The stack-like contacting leads to gas transport conditions different from the ideal case which is investigated at the fuel and air side with modified gas diffusion coefficients by using gas mixtures containing nitrogen and helium as well [2].The positive effect of the protective coating towards the cell performance is evaluated by current-voltage characteristics and EIS measurements. Further, the electrode electrochemistry influenced by effects like chromium-poisoning will be shown and compared to the ideal contact set-up [2].

Photoacoustic Spectral Response using Ultrasound and Interferometric Sensors: A Correlation Study for a High Bandwidth Real-Time Blood Vasculature Monitoring Application in a Chick-Embryo Chorioallantoic Membrane (CAM) Model.

Photoacoustic (PA) spectral response technique has shown good promise in efficient preclinical tissue diagnosis by depicting mechano-biological properties due to high spatial resolution and penetration depth. The conventional PA-based system is a pump-probe technique that utilizes neodymium-doped yttrium aluminum garnet pulsed laser as a pump and an ultrasound sensor as a probe. For biomedical studies, high-speed PA signals need to be acquired, requiring higher bandwidth ultrasound sensors. While the bandwidth increases, they exhibit a very low signal-to-noise ratio that inhibits acquiring PA signals of biomedical samples. An interferometer-based probe has recently been investigated as a potential ultrasound probe for obtaining PA signals as an alternative. This optical PA detection technique offers high sensitivity by combining low acoustic impedance with high electromechanical coupling. However, there is a lack of exploration of the same for real-time biomedical studies. This work shows the development of a homodyne Mach-Zehnder interferometer-based PA spectral response (PASR) followed by a correlation study between the conventional ultrasound sensor and the interferometer-based sensor. Further, this study demonstrates the capability of continuous monitoring of vascular growth and the effect of an antidrug (Cisplatin) on the vasculature tested on a chick-embryo chorioallantoic membrane model. PASR was able to monitor growth changes within one day, which was not possible with conventional methods. This opens up potential possibilities for using this technique in biomedical applications.

Molecular recognition of trehalose and trehalose analogues by Mycobacterium tuberculosis LpqY-SugABC

Trehalose plays a crucial role in the survival and virulence of the deadly human pathogen Mycobacterium tuberculosis (Mtb). The type I ATP-binding cassette (ABC) transporter LpqY-SugABC is the sole pathway for trehalose to enter Mtb. The substrate-binding protein, LpqY, which forms a stable complex with the translocator SugABC, recognizes and captures trehalose and its analogues in the periplasmic space, but the precise molecular mechanism for this process is still not well understood. This study reports a 3.02-Å cryoelectron microscopy structure of trehalose-bound Mtb LpqY-SugABC in the pretranslocation state, a crystal structure of Mtb LpqY in a closed form with trehalose bound and five crystal structures of Mtb LpqY in complex with different trehalose analogues. These structures, accompanied by substrate-stimulated ATPase activity data, reveal how LpqY recognizes and binds trehalose and its analogues, and highlight the flexibility in the substrate binding pocket of LpqY. These data provide critical insights into the design of trehalose analogues that could serve as potential molecular probe tools or as anti-TB drugs.