Potential Of Saliva Research Articles

A highly sensitive 3base™ assay for detecting Streptococcus pyogenes in saliva during controlled human pharyngitis

Streptococcus pyogenes (Group A Streptococcus; GAS) is a Gram-positive bacterium responsible for substantial human mortality and morbidity. Conventional diagnosis of GAS pharyngitis relies on throat swab culture, a low-throughput, slow, and relatively invasive ‘gold standard’. While molecular approaches are becoming increasingly utilized, the potential of saliva as a diagnostic fluid for GAS infection remains largely unexplored. Here, we present a novel, high-throughput, sensitive, and robust speB qPCR assay that reliably detects GAS in saliva using innovative 3base™ technology (Genetic Signatures Limited, Sydney, Australia). The assay has been validated on baseline, acute, and convalescent saliva samples generated from the Controlled Human Infection for Vaccination Against Streptococcus (CHIVAS-M75) trial, in which healthy adult participants were challenged with emm75 GAS. In these well-defined samples, our high-throughput assay outperforms throat culture and conventional qPCR in saliva respectively, affirming the utility of the 3base™ platform, demonstrating the feasibility of saliva as a diagnostic biofluid, and paving the way for the development of novel non-invasive approaches for the detection of GAS and other oropharyngeal pathogens.

The difference Diagnosis criteria for PTSD

Post-Traumatic Stress Disorder (PTSD) is a debilitating mental health condition that significantly affects individuals who have experienced trauma. PTSD has substantial impact on mental, emotional, and physical well-being, encompassing emotional distress, impaired social functioning, cognitive deficits, adverse physical health outcomes, and even suicidal ideation. Early diagnosis of PTSD is vital, given its profound consequences. The diagnostic process is intricate and involves diverse criteria and assessment tools. This research explores these diagnostic methods, focusing on the criteria outlined in the Diagnostic and Statistical Manual of Mental Disorders (DSM) and the International Classification of Diseases (ICD). Furthermore, it emphasizes the importance of self-rating scales and a groundbreaking discovery related to saliva as a potential diagnostic marker for PTSD. The evolution of diagnostic criteria, diagnostic frameworks in the DSM-5 and ICD-11, self-rating scales like the PTSD Checklist (PCL), and the potential of saliva as a diagnostic criterion are discussed. These diagnostic tools offer valuable insights for the assessment and diagnosis of PTSD, enhancing treatment and support for affected individuals.

Perception of Fomite Route of Transmission, Usage of Masks and Role of Saliva in coronavirus Disease-19 Infections: A Cross-sectional Survey among Dental Health Care Professionals in Saudi Arabia

Background: Coronavirus Disease-19 has been spreading globally with the rise of many variants after being declared as a pandemic on 11th March 2020. There is a lack of scientific literature focused on fomite transmission of COVID-19, especially the potential of saliva as a contributing factor in the spread and diagnosis of COVID-19 in dental healthcare practice. Hence, the aim was to comprehend the level of awareness of dental health care professionals/practitioners (DHCPs) about the role of fomite route of transmission and the role of saliva in Coronavirus Disease-19 (COVID-19) infections. Methods: An electronic questionnaire using Google Forms was circulated online after ethical approval and validation to dental health practitioners in Saudi Arabia, via emails and social media platforms. Results: Three hundred and eighty-nine responses were recorded. The female DHCPs had statistically significant awareness of the fomite route of transmission among various dental health professionals (p=0.04) and dental students, and dental interns had the maximum awareness (p<0.05). The DHCPS preferred to learn and collect nasopharyngeal specimens by themselves for COVID-19 testing. Doctoral DHCPs had maximum awareness about WHO guidelines for social distancing, while dental nurses were more informed about infection control methods against COVID-19. Conclusion: DHCPs have adequate information about the fomite route of transmission and the role of saliva in COVID-19. Saliva specimen collection is specifically preferred for COVID-19 testing by dental assistants and hygienists for testing in the dental health center/clinic/university. The DHCPs and dental hygienists were less aware of the fomite route of transmission through asymptomatic COVID-19 infected patients.

Point-of-care salivary oxidative and renal functional markers to assess kidney function in reperfusion-induced acute kidney injury in male rats.

Saliva is one of the most promising body fluids in the research of new biomarker for various diseases diagnosis. However, serial sampling in this condition is very dangerous and pose iatrogenic anemia with blood loss. This study was done to evaluate the cost-effectiveness of point-of-care salivary tests and identify the validity of salivary markers. Rats were randomly assigned to four experimental groups: (1) control (2) IR-3h (3) IR-6h (4) IR-24h. Both renal pedicles were occluded for 55min and then were declamped to allow reperfusion for 3, 6 and 24h in IR groups. After reperfusion, all rats received pilocarpine 1mg/kg to collect saliva. Plasma samples were also collected. Renal parameters including Cr, uric acid, and urea, malondialdehyde (MDA) levels, Bax/Bcl2 ratio, nitrite/nitrate ratio, corticosterone levels and oxidant/antioxidant ratio were measured in both plasma and salivary samples. There were significant increased level of renal function parameters, MDA levels, Bax/Bcl2 ratio, nitrite/nitrate ratio and corticosterone in both saliva and plasma. The comparison of above parameters in both saliva and plasma showed significant correlation. This study demonstrated that concentrations of indices specifically renal functional parameters increase in saliva in the IR-induced kidney injury in male rats and result indicate the potential of saliva as a tool to monitoring AKI. Measurement of salivary parameters may can become reliable diagnostic tests for patients with AKI.

Human Saliva as a Diagnostic Specimen for Early Detection of Inflammatory Biomarkers by Real-Time RT-PCR.

Nowadays human saliva is more frequently studied as a non-invasive, stress-free, and preferable diagnostic material than blood. Supporting evidences acknowledge saliva as a mirror that reflects the body’s physical state. Numerous studies have also demonstrated the presence and use of RNA derived from saliva in the early diagnosis of disease by real-time reverse transcriptase polymerase chain reaction (RT-PCR). Assessing the host inflammatory response in patients and its resolution at an early stage can serve as a prognostic and predictive method in determining therapeutic response or disease progression. In this context, the potential of saliva as a specimen to diagnose early inflammatory biomarkers using RT-PCR seems fascinating and useful. Here, we review inflammatory biomarkers within the saliva, focusing on early detection of these biomarkers using RT-PCR and the factors influencing the quality of saliva specimen.

Mинерализирующий потенциал смешанной слюны и течение кариеса зубов у детей и подростков с эндокринной патологией и патологией опорно-двигательной системы

The features of microcrystallization and the mineralizing potential of mixed saliva, the intensity of caries in children and adolescents with endocrine pathology and pathology of the musculoskeletal system at the age of 9–10, 12–13 and 16–17 years have been studied, the relationship between the intensity of dental caries and the mineralizing potential of saliva has been revealed. The study of saliva in patients with endocrine pathology showed a low saliva mineralizing potential, crystals of type II and III were found, with a predominance of type III. The lowest mineralizing potential of saliva is observed at the age of 12–13 years. The carious process progresses with age and the subcompensated degree of activity prevails. The study of mixed saliva in patients with pathology of the musculoskeletal system showed a low mineralizing potential, crystals of types II and III were encountered, with a predominance of type III. The lowest mineralizing potential of saliva is observed at the age of 12–13 years. The carious process stabilizes over time and compensated degrees of activity prevail. The examinees with endocrine and musculoskeletal pathologies have reduced salivary mineralizing potential, high rates of intensity and degree of caries activity. In patients with endocrine pathology, indicators are worse than in patients with musculoskeletal pathology. The age of 12–13 is critical.

EVALUATION OF THE MINERALIZING POTENTIAL OF ORAL FLUID IN CHILDREN WITH DOWN SYNDROME DEPENDING ON THE LEVEL OF THE CARIOGENIC PROCESS

Early diagnosis of dental caries in children is difficult. This fact is applied to children with Down syndrome. In order to identify predictors of dental diseases, a crystallographic method is widely used for studying oral fluid. The aim of the work is to evaluate the mineralizing potential of oral fluid in children with Down syndrome before and after the course of preventive measures. Material and methods. We examined oral fluid in children with Down syndrome. Children and adolescents at the age of 8 months to 17 years (n=21) were divided into groups depending on the age and the level of caries intensity. The results were compared with the data of 55 children without chromosomal pathology. Results. The group of children with Down syndrome (MG), during the initial examination includes type II – 11 people (53 %) and type III – 7 people (33 %) microcrystallization of saliva, which generally corresponds to the situation in the group of children without chromosomal pathology (CG), where type II included 62 % (34 people) and type III – 22 % (12 people). The average value of the mineralizing potential of saliva in the main group was 2.78±0.22, and in the control group – 2.89±0.17. Conclusions. After preventive measures during the re-examination of children with Down syndrome, there was a tendency to an increase in the level of parameters of the mineralizing potential of saliva while maintaining this level after 6 months.

The comparison of cleaning and remineralizing properties of toothpastes with and without surface-active substances

Objective : Analysis of surface-active substances (SAS) effect on cleaning properties of toothpastes and remineralizing potential of saliva. Methods : We have assessed clinical indices as well as biochemical and microbiological parameters of oral fluid when using toothpastes with and without SAS. Results : Statistically significant changes when using various toothpastes by the 28th day were registered only for simplified oral hygiene index. The presence of SAS in the composition of toothpastes did not affect the total number of microorganisms in the composition of dental plaque (in the change in the number of individual species). There were no significant differences in the saturation of the oral fluid with Ca 2+ and PO 4 3– ions. Conclusions : SAS (sodium lauryl sarcosinate) using in toothpastes composition has a significant effect on cleaning properties of oral hygiene products while not having a special effect on the mineralizing potential of saliva.

ITRAQ-based quantitative analysis of age-specific variations in salivary proteome of caries-susceptible individuals

BackgroundHuman saliva is a protein-rich, easily accessible source of potential biomarkers for the diagnosis of oral and systemic diseases. However, little is known about the changes in salivary proteome associated with aging of patients with dental caries. Here, we applied isobaric tags for relative and absolute quantitation (iTRAQ) in combination with multiple reaction monitoring mass spectrometry (MRM-MS) to characterize the salivary proteome profiles of subjects of different ages, presenting with and without caries, with the aim of identifying age-related biomarkers for dental caries.MethodsUnstimulated whole saliva samples were collected from 40 caries-free and caries-susceptible young adults and elderly individuals. Salivary proteins were extracted, reduced, alkylated, digested with trypsin and then analyzed using iTRAQ-coupled LC–MS/MS, followed by GO annotation, biological pathway analysis, hierarchical clustering analysis, and protein–protein interaction analysis. Candidate verification was then conducted using MRM-MS.ResultsAmong 658 salivary proteins identified using tandem mass spectrometry, 435 proteins exhibited altered expression patterns in different age groups with and without caries. Of these proteins, 96 displayed age-specific changes among caries-susceptible adults and elderly individuals, and were mainly associated with salivary secretion pathway, while 110 age-specific proteins were identified among healthy individuals. It was found that the age factor caused significant variations and played an important role in both healthy and cariogenic salivary proteomes. Subsequently, a total of 136 target proteins with complex protein–protein interactions, including 14 age-specific proteins associated with caries, were further successfully validated using MRM analysis. Moreover, non-age-specific proteins (histatin-1 and BPI fold-containing family B member 1) were verified to be important candidate biomarkers for common dental caries.ConclusionsOur proteomic analysis performed using the discovery-through-verification pipeline revealed distinct variations caused by age factor in both healthy and cariogenic salivary proteomes, highlighting the significance of age in the great potential of saliva for caries diagnosis and biomarker discovery.

Advancing Point-of-Care (PoC) Testing Using Human Saliva as Liquid Biopsy.

Salivary diagnostics is an emerging field for the encroachment of point of care technology (PoCT). The necessity of the development of point-of-care (PoC) technology, the potential of saliva, identification and validation of biomarkers through salivary diagnostic toolboxes, and a broad overview of emerging technologies is discussed in this review. Furthermore, novel advanced techniques incorporated in devices for the early detection and diagnosis of several oral and systemic diseases in a non-invasive, easily-monitored, less time consuming, and in a personalised way is explicated. The latest technology detection systems and clinical utilities of saliva as a liquid biopsy, electric field-induced release and measurement (EFIRM), biosensors, smartphone technology, microfluidics, paper-based technology, and how their futuristic perspectives can improve salivary diagnostics and reduce hospital stays by replacing it with chairside screening is also highlighted.

Correlation of Plasma Lipid Profile with Salivary Oxidative Stress Markers in Type II Diabetes Mellitus Patients.

Diabetes is known to be associated with greater production of reactive oxygen species (ROS) like malondialdehyde (MDA) and decreased antioxidants like total thiols and its estimation in plasma is used in monitoring the redox status. The aim of this study was to analyse the association between plasma lipid profile parameters like HDL and LDL with salivary MDA and thiols in diabetic patients. Sixty subjects between the age group 35- 70 years who were diagnosed with type II diabetes mellitus based on fasting blood glucose and glycated haemoglobin levels who attended the dental OPD at Kasturba Medical College and Hospital, Manipal consented to participate in this study. Plasma glucose, HDL and LDL were estimated in Cobas autoanalyser by hexokinase method, homogenous enzymatic colorimetric assay and Friedwald's formula respectively. Assessment of glycated hemoglobin was by ion exchange chromatography, MDA by thiobarbituric acid as a substrate and thiols by Ellmann's manual method in plasma and saliva. The association of plasma LDL with salivary MDA was found to be positive and significant and that with salivary thiols was negative and significant. Also, the association of plasma HDL with salivary MDA was found to be negative and significant and that with salivary thiols was positive and significant. RESULTS indicate the potential of saliva as a tool to monitor prognosis of diabetes.

The potential of saliva in protecting against dental erosion.

Saliva is the most relevant biological factor for the prevention of dental erosion. It starts acting even before the acid attack, with an increase of the salivary flow rate as a response to the acidic stimuli. This creates a more favorable scenario, improving the buffering system of saliva and effectively diluting and clearing acids that come in contact with dental surfaces during the erosive challenge. Saliva plays a role in the formation of the acquired dental pellicle, a perm-selective membrane that prevents the contact of the acid with the tooth surfaces. Due to its mineral content, saliva can prevent demineralization as well as enhance remineralization. These protective properties may become more evident in hyposalivatory patients. Finally, saliva may also represent the biological expression of an individual's risk for developing erosive lesions; therefore, some of the saliva components as well as of the acquired dental pellicle can serve as potential biomarkers for dental erosion.

Salivary shedding of HHV-8 in people infected or not by human immunodeficiency virus 1

Human herpesvirus 8 (HHV-8), the main agent involved in the etiopathogenesis of Kaposi's sarcoma (KS) is primarily transmitted through sexual contact. The potential of saliva as a source of HHV-8 transmission remains unclear. The purpose of this work was to determine the frequency of HHV-8 detection in saliva of HIV-infected individuals and their family contacts. The study group comprised 210 individuals. Group 1: 35 HIV-infected patients; group 2: 35 non-HIV individuals; group 3: two siblings for each patient from group 1; group 4: two siblings for each individual from group 2. Each participant had non-stimulated whole saliva collected and DNA was extracted. HHV-8-DNA amplification from ORF-26 was performed using a nested PCR protocol. HHV-8 DNA was detected in saliva from 14/35 (40%) HIV-infected individuals and 4/35 (11.4%) non-HIV-infected individuals (OR = 5.16, CI [1.49-17.88], P = 0.006). It was also possible to amplify HHV-8 DNA in 11/70 (15.7%) relatives of HIV-infected participants and 4/70 (5.71%) relatives of non-HIV-infected individuals(P = 0.041). Among the 14 group 1 patients with HHV-8 DNA detected in saliva, eight (57.1%) had a household member in whom HHV-8 DNA was also amplified (OR = 8, CI [1.58-40.29] P = 0.007). HHV-8 DNA is frequently found in saliva. HIV-infected individuals showed a higher frequency of detection of HHV-8 than healthy controls. HHV-8 DNA was significantly amplified in saliva of household members of HIV/HHV-8 co-infected individuals.

Negative Ion Chemical Ionization Mass Spectrometry for the Analysis of 3,5,6‐Trichloro‐2‐pyridinol in Saliva of Rats Exposed to Chlorpyrifos

Organophosphorus (OP) insecticides (e.g. chlorpyrifos) are widely used in a variety of applications, and the potential exists for significant occupational and environmental exposures. They have been associated with more occupational poisoning cases than any other class of insecticides. One of the best approaches for accurately assessing human dosimetry and determining risk from both occupational and environmental exposure is biomonitoring. Biological matrices such as blood and urine have been routinely used for biomonitoring; however, other matrices such as saliva represent a simple and readily obtainable fluid. As a result, saliva has been suggested as an alternative biological matrix for the evaluation of a broad range of biomarkers such as environmental contaminants, drugs of abuse, hormones, chemotherapeutics, heavy metals, and pesticides. Chlorpyrifos (CPF), and its major metabolite, 3,5,6‐trichloro‐2‐pyridinol (TCP), have been quantified in urine and blood as a biomarker for exposure to OP insecticides. The purpose of this study was to develop an analytical approach for detecting and quantitating the levels of TCP in saliva obtained from rats exposed to CPF and to evaluate the potential of saliva as a noninvasive biomonitoring matrix for the determination of exposure to OP insecticides. Adult male rats were administered CPF, and blood and saliva were humanely collected for analysis of TCP and CPF. The TCP was detected and quantitated in saliva using negative ion chemical ionization mass spectrometry with selected ion monitoring. Initial results indicate that saliva potentially may be utilized as a noninvasive biomonitoring matrix to determine exposure to organophosphate insecticides. The authors would like to thank Ms. K. Brzak and Dr. M. Bartels of Dow Chemical Company for their helpful input. This work was partially supported by the U.S. Environmental Protection Agency's STAR program through grant R828608. It has not been subject to any EPA review and, therefore, does not necessarily reflect the views of the agency, and no public endorsement should be inferred. This publication was also partially supported by grant 1 R01 OH03629‐01A2 from the Centers for Disease Control and Prevention (CDC). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the CDC.

Sialochemistry in diseases and clinical situations affecting salivary glands.

AbstractDuring the past few years an increasing number of investigators in a variety of disciplines have become interested in the potential of saliva as a diagnostic tool. The increase in interest reflects (in part at least) the marked proliferation in information on salivary physiology and salivary chemistry. Sialochemistry is being applied to (1) diseases of the salivary glands per se with no evidence of systemic involvement, (2) systemic diseases in which salivary glands are involved, and (3) clinical situations in which salivary flow rate and chemistry are helpful in diagnosis or monitoring patient progress (Tables 1 and 2).