Perspective the use of space tourism in rural area, but not only in this area, must be based on the concept of ecological space based on the principle of equity, then an area needs to be provided in-house and in conditions of sustainable development. Sustainable human development can not be conceived outside the human settlements development of a viable economy and a diversified social life in a healthy environment, ensured respect cultural heritage - historical society. Numerous researches have shown that ecotourism can be a viable alternative for economic development of Romanian rural communities. This research aims to study ecotourism in the context of sustainable development of rural Romanian areas and conditions to protect its natural resources. This category of natural resources now put the following problems: exploitation beyond the regeneration, especially in soils and forests, pollution of the environment that minimizes the potential for regeneration. Having as main source of information national and international literature and data provided by the National Institute of Statistics of Romania, was created a pretty thorough fundamental research in specific field. The main research method was used were: bibliographical study, literature review and synthesis, collection and processing of statistical data, etc. Environmental protection has been considered as a basic problem in tourism since the beginning of this activity in our country. Ecological attitude towards natural resources exploitation involves giving unlimited and irrational generalization about the importance of scientific concept of biosphere preservation of natural resources for future generations. In the spirit of these principles requires development of ecological strategies of development (eco-development), capable of providing a healthy and rational use of resources specific to a particular ecosystem, to meet basic human needs. Protected areas are, in part, and highly attractive tourist destinations such as Danube Delta Biosphere Reserve, National Parks and other natural reserves, except, of course, scientific reserves (strictly protected). Protection and conservation of rural tourism potential is emerging as a separate issue, which requires collaboration of specialists in various fields. Starting from the concept that "sustainable development of viable and sustainable ecological development that is considered to meet the needs of the present without compromising the ability of future generations to meet their own needs" tourism, especially ecotourism, as an economic activity, it is this scientific approach and should cooperate with other industries and economic activities to ensure environmental quality, resource base and its survival. More so, it becomes necessary co-participation, to this end, the central and local authorities, local communities, along with tourism providers and related services.