Horticultural Potential Research Articles

INTRODUKSI PROSES PENGOLAHAN PANGAN BERBASIS TANAMAN HORTIKULTURA UNTUK PEMBERDAYAAN MASYARAKAT DESA WUNUT KABUPATEN KLATEN

Wunut Village is located in Tulung Sub-District, Klaten Regency which has the horticultural potential such as tomato and chili. But the huge potential has not been able to lift the economy of the village community. Therefore the community devotion team of Department of Food Science and Technology, Faculty of Agriculture, Sebelas Maret University chose Wunut Village as the target village. The problems faced by farming communities in Wunut Village are (1) production of chili and tomatoes are abundant, but the selling price is relatively low; (2) the large number of poor people; (3) lack of knowledge and community insight about entrepreneurship in the field of food; (4) interest in entrepreneurship is still lacking in society; And (5) the difficulty of marketing the resulting product. The solutions offered by the community devotion team are through training activities and the introduction of tomato processing technology into “torakur”; Training and introduction of production process technology of shredded pepper; Entrepreneurship training and food industry management; Information technology-based marketing training and direct selling strategies. In order to support these activities, aids were also provided to improve the quality of the chakra and the resulting chili pepper. Equipment provided in the form of cabinet dryer, dry mill, and packer sealer. The result of this activity in the form of introduction of tomato processing technology into “torakur”, and processing of chili into shredded chili has been done well, with products that can be accepted by society and have a big prospect to be developed.<br />Keywords:entreprenuership, quality, torakur, shredded chili, Wunut Village

Vision of COST BioGreenhouse

Good food is the basis of a healthy, active life. Horticulture provides fresh commodities rich in minerals, fibres and bioactive compounds, and thereby contributes to a healthy diet. Integrated and organic horticultural production, either indoors or outdoors, contribute to food provisioning. For food and nutrition security, food must be: 1) present in sufficient quantities, 2) physically, economically and socially accessible and affordable, 3) nutritious, with a quality that meets dietary needs and preferences, 4) safe and 5) supported by an adequate environment regarding sanitation, health service and care. To pursue the ambition of developing organic horticulture in greenhouses and high tunnels into an active player within the food system and foodservice, a shift in paradigm is needed - organic protected horticulture needs to redefine itself from being a resource provider/supplier, mainly focusing on production systems and environment, to being an active player in the arena of grand global challenges and human health-integrating societal aspects, e.g., well-being. Organic greenhouse horticulture not only addresses, but also delivers, key solutions in some areas of conflicts, such as land, water and energy use and recycling of resources. In addition, it offers opportunities for empowerment of rural and urban areas. Most research on organic horticulture considers organic greenhouse production systems and production environments. The BioGreenhouse vision considers interactivities between the environmental and societal sphere of food. Innovative organic approaches are a critical precondition for improvement. The potential of organic greenhouse horticulture needs to be brought to the awareness of policy makers. Research to explore the multilevel and multidisciplinary as well as multistakeholder interactions of organic production in greenhouses and high tunnels must be encouraged, by all necessary means.

GBS-Based Deconvolution of the Surviving North American Collection of Cold-Hardy Kiwifruit (Actinidia spp.) Germplasm.

Plant germplasm collections can be invaluable resources to plant breeders, provided they are well-characterized. After 140 years of acquisition and curation efforts by a wide and largely non-coordinated array of private and institutional actors, the current US collection of cold-hardy kiwifruit (Actinidia spp.) is rife with misclassifications, misnomers, and mix-ups. To facilitate the systematic improvement and resource-efficient curation of these species of long-recognized horticultural potential, we used genotyping-by-sequencing (GBS) data to deconvolute this historic collection. Evaluation of a total of 138 accessions (103 A. arguta, 28 A. kolomikta, and 7 A. polygama) with an interspecific set of 1,040 high-quality SNPs resulted in clear resolution of the three species. Intraspecific analysis (2,964 SNPs) within A. arguta revealed a significant level of redundancy (41.7%; only 60 unique genotypes out of 103 analyzed) and a sub-population structure reflecting likely geographic provenance, phenotypic classes, and hybrid pedigree. For A. kolomikta (3,425 SNPs), the level of accession redundancy was even higher (53.6%; 13 unique genotypes out of 28 analyzed); but no sub-structure was detected. Numerous instances were discovered of distinct genotypes sharing a common name, different names assigned to the same genotype, mistaken species assignments, and incorrect gender records, all critical information for both breeders and curators. In terms of method, this study demonstrates the practical and cost-effective use of GBS data to characterize plant genetic resources, despite ploidy differences and the lack of reference genomes. With the recent prohibition on further imports of Actinidia plant material into the country and with the active eradication of historic vines looming, this analysis of the US cold-hardy kiwifruit germplasm collection provides a timely assessment of the genetic resource base of an emerging, high-value specialty crop.

850. MYRCEUGENIA LEPTOSPERMOIDES

SummaryThough extremely rare in British and Irish gardens, the South American genus Myrceugenia shows great horticultural potential and a number of species have entered cultivation in the last four decades, primarily through the efforts of the Royal Botanic Garden, Edinburgh. Two of the showiest of these, Myrceugenia lanceolata and Myrceugenia leptospermoides, both Chilean endemics, are discussed.

820. STAUNTONIA LIBERA

SummaryStauntonia libera, collected as ‘KWJ 12218’ from Vietnam and cultivated by Crûg Farm Plants, is an evergreen vine with scented flowers and edible fruit. It is described and illustrated here and details of the distribution and habitat are also provided. It is a plant with horticultural potential, but remains a rarity in cultivation.

Micropropagation of a giant ornamental bromeliad Puya berteroniana through adventitious shoots and assessment of their genetic stability through ISSR primers and flow cytometry

An efficient micropropagation protocol has been developed for Puya berteroniana, a giant Chilean bromeliad with attractive turquoise flowers and great horticultural potential. Plant material for the experiment was multiplied via repeated sub-cultures of adventitious shoots, originally derived from a single in vitro germinated seedling, on a half-strength Murashige and Skoog (MS) medium (Murashige and Skoog in Physiol Plant 15:473–497, 1962) containing 0.44 µM 6-benzyladenine (BA). For the in vitro propagation experiment, the shoots were cultured on a half-strength MS medium supplemented with BA (0.04–2.22 µM) or zeatin (0.05–2.28 µM) alone or in combination with α-naphthaleneacetic acid (NAA) (0.54 µM). The maximum shoots per explant (5.5), was obtained on a medium containing 0.44 µM BA. Rooting of the shoots was tested on a medium supplemented with NAA (0.54–2.69 µM) or indole-3-acetic acid (0.57–2.85 µM). The best rooting was achieved on a medium containing 2.69 or 1.61 µM NAA. The rooted plantlets were transferred ex vitro, with 98.3 % survival rate. Inter simple sequence repeat (ISSR), flow cytometry, and karyological analysis were used to evaluate true-to-type of the in vitro regenerants. Ten randomly chosen plants and control plant were used. Twenty ISSR primers produced 95 clear, distinct, and reproducible bands per analysed sample. All amplified products were monomorfic, and no polymorphism was detected. Similarly, flow cytometric analysis confirmed that the ploidy level in all plantlets was stable. Karyological analysis revealed number of somatic chromosomes 2n = 50. As no somaclonal variation was detected in culture, this micropropagation protocol can be used for the mass production of P. berteroniana plants.

Evaluation of Total Phenolic Contents and Antioxidant Activity of Carrot Callus Extracts as Affected by Phenylalanine Precursor

Carrot (Daucus carota) is a valuable plant with both therapeutic and horticultural potential. Stem, petiole and root derived calli of carrot were obtained on solid MS supplemented with 1 mg/l BAP + 2 mg/l NAA. Callus cultures supplemented with different L-phenylalanine (PHE) concentrations under light and dark conditions were evaluated for their antioxidant activity and total phenolic contents. The authors showed that PHE supplementation in Daucus carota cultures was necessary to raise the extraction yield percentage. Antioxidant assays such as DPPH scavenging activity and ?-carotene bleaching have been carried out. In DPPH system, callus extracts from different explants grown under light conditions displayed lower DPPH radical scavenging activity at all PHE levels compared with that grown under dark conditions. Moreover, under both light and dark conditions callus cultures grown on MS supplemented with 1 mg/l BAP + 2 mg/l NAA plus 1000 mg/l PHE were recorded to yield the maximum values as antioxidant activities. Regarding ?-carotene bleaching assay, under light condition the callus extract of stem, root and petiole recorded an inhibition of linolic acid 47.9, 41.43 and 39%) which is lower compared with dark grown cultures, respectively (52.46, 72.71 and 73.26%). Effect of different concentrations of phenylalanine on the total phenolic content of carrot callus extract examined under light conditions varied from 0.33 to 2 mg/g DW and 0.51 to 3.69 mg/g DW under dark conditions as expressedPlant Tissue Cult. & Biotech. 25(2): 207-221, 2015 (December)

Phenotypic and Genotypic Analysis of a U.S. Native Fine-leaved Festuca Population Reveals Its Potential Use for Low-input Urban Landscapes

Continued reduction in limited natural resources worldwide increasingly necessitates the incorporation of low-maintenance and low-input plant materials into urban landscapes. Some fine-leaved Festuca grass species have been used in formal gardens and native urban landscapes because of their inherent tolerance to abiotic stresses, but native, ornamental types (tall and non-spreading with multicolored culms and panicles) are not common in landscapes of the western United States. A native fine-leaved Festuca collection made in Montana (designated FEID 9025897) by the U.S. Natural Resources Conservation Services possesses such ornamental characteristics but has not been evaluated for its horticultural potential. Therefore, a study was designed to assess its phenotypic and genotypic attributes by cloning 270 FEID 9025897 plants and evaluating them along with native F. idahoensis and F. ovina PIs (five) and commercial checks (five) for genetic diversity and plant morphology for 2 years (2010–11). Plant genetic constitution was determined using amplified fragment length polymorphism (AFLP) analysis. Plant height, width, biomass, relative vigor (visual rating of 0 = dead to 5 = green, abundant growth), persistence (number of plants alive per plot), and regrowth after clipping (visual rating of 0 = none to 5 = most) were estimated by evaluation of plants under replication at Hyde Park, UT. Based on AFLP-based coancestry analysis, FEID 9025897 plants possessed considerable genetic affinities with F. idahoensis. Morphological traits as averaged over both years varied in height (13.9 to 105.0 cm), width (9.9 to 66.2 cm), biomass (0 to 170.4 g), vigor (0.2 to 4.7), persistence (0 to 3.9), and regrowth (0 to 4.0). Based on these differences, 19 (7%) FEID 9025897 plants were identified for their ornamental potential that possessed multicolored (red, orange, and yellow) culms and varied in morphology with 2-year means of height (79.8 cm), width (45.2 cm), biomass (88.5 g), vigor (2.9), persistence (1.8), and regrowth (3.7).

Diversity, distribution and horticultural potential of Cordia myxa L.: a promising underutilized fruit species of arid and semi arid regions of India

Cordia myxa L. locally known as Gonda, Lasora or lehsua belongs to family Boraginaceae. It grows throughout India except in high hills and temperate climates. This is a perennial, medium sized tree with crooked stem. It bears small sized fruits in bunches, used as traditional vegetable and pickles. Besides, it has number of other uses including medicine, fuel wood and ornamental wood work. Due to its drought tolerance ability, it is abundantly found in arid and semi arid regions of North and North West India. Its cultivation as planned orchards is limited and only scanty information about various aspects of the species is available. This paper therefore, deals with synthesised information on its distribution, taxonomy, traditional knowledge and experimental findings on diversity, propagation, improvement and utilization. The information on production and consumption may open new vistas in dietary supplement, thus opening a new window of commercial opportunity in arid and semi arid regions of India and isoclimatic regions of the world.

Plant growth regulator induced phytochemical and antioxidant variations in micropropagated and acclimatized Eucomis autumnalis subspecies autumnalis (Asparagaceae)

Eucomis species is a valuable plant with both medicinal and horticultural potential. The current study evaluated the role of plant growth regulator (PGR) on growth, phytochemicals, and antioxidant activity in Eucomis autumnalis subspecies autumnalis. Five cytokinins including topolins and benzyladenine (BA) at 2 µM in combination with varying (0–15 µM) concentrations of naphthalene acetic acid (NAA) were tested. In vitro regenerants were acclimatized in the greenhouse for 4 months. Highest number of shoots (9 shoots/explant) was observed with 15 µM NAA alone or when combined with BA. Acclimatized plants derived from the 15 µM NAA treatment had the highest number of roots, largest leaf area and biggest bulbs. While applied PGRs increased the iridoids and condensed tannins in the in vitro regenerants, total phenolics and flavonoids were higher in the PGR-free treatment. Among the in vitro regenerants, 5 µM NAA and 2 µM BA treatments produced the best antioxidant activity in the DPPH (55 %) and beta-carotene (88 %) test systems, respectively. A remarkable carry-over effect of the PGR was conspicuous in the phytochemical levels and antioxidant activity of the 4-month-old plants. In addition to the optimized micropropagation protocols, the current findings present a promising potential for manipulating the type and concentration of applied PGRs to improve phytochemical production and hence medicinal value in E. autumnalis subspecies autumnalis.

Environmental Control of Flowering in Pennsylvania Sedge

Pennsylvania sedge (Carex pensylvanica) is an upland forest sedge with restoration and horticultural potential as a low-maintenance groundcover for dry shade. For large landscape and restoration plantings, seed or achenes in this case are much preferred due to lower labor and material costs. However, pennsylvania sedge typically produces few achenes in its native habitat. As a first step in improving achene production, this research evaluated the effect of vernalization and photoperiod on floral initiation and development. We conclude that this sedge is an obligate short-day plant that does not require vernalization for flowering. Plants flowered when exposed to daylengths of 6 to 12 hours. Flowering was completely inhibited with 14-hour photoperiods. Pennsylvania sedge was florally determined after 4 weeks of 8-hour photoperiods. Inflorescence quantity and normal floral development varied by clone and by weeks of exposure to 8-hour photoperiods. For two of the clones, the largest number of normal monoecious inflorescences was produced with 8 to 10 weeks of 8-hour photoperiods while the other two clones only required 6 to 8 weeks of exposure to inductive photoperiods. Therefore, it is important to evaluate observable variation between clones when attempting to propagate pennsylvania sedge.

Physiological and phytochemical responses of three nutrient-stressed bulbous plants subjected to vermicompost leachate treatment

The role of vermicompost leachate (VCL) on growth, photosynthetic pigments, phytochemical content and the bioactivities of three bulbous (Eucomis autumnalis, Tulbaghia ludwigiana and Tulbaghia violacea) plants with medicinal and horticultural potential under nutrient-stressed conditions was evaluated. After 15 weeks, the nutrient-stressed plants were harvested and growth parameters were recorded. Spectrophotometric methods were used to quantify the photosynthetic and phytochemical contents. Harvested plant materials were extracted with 50 % methanol and subjected to antioxidant, acetylcholinesterase inhibitory (AChEI) and antifungal bioassays to elucidate the effect of the applied VCL under nutrient stress. While VCL did not significantly enhance growth of the three species, it significantly increased photosynthetic pigment content in 50 % Hoagland’s-treated Eucomis autumnalis and in phosphorus-deficient Tulbaghia ludwigiana. Among the quantified phytochemicals, the most promising finding was the significant increase in condensed tannin and iridoid contents in VCL-supplemented-nutrient-stressed Tulbaghia violacea. Better antioxidant (Eucomis autumnalis) and AChEI (Eucomis autumnalis, Tulbaghiaviolacea) activities were detected when VCL was applied under nutrient stress. The current findings indicate that the application of VCL holds potential in cultivation of plants under nutrient-stressed conditions.

Phenotypic and genetic characterization of Kyrgyz fine-leaved Festuca valesiaca germplasm for use in semi-arid, low-maintenance turf applications

Fine-leaved Festuca valesiaca Schleich. ex Gaudin (2n = 2x–4x) is native to heavily-grazed, cold, semi-arid, Asian rangelands. However, its potential for low-maintenance turf applications in the semi-arid western United States and its relatedness to other agriculturally important Festuca species have not been investigated. Therefore, a project was designed to identify F. valesiaca accessions that possess horticultural potential when grown under semi-arid growing conditions and to characterize their relatedness to other Festuca species. In 2008, 12 F. valesiaca accessions originating from Kyrgyzstan and eight US. Festuca and one Lolium cultivar were transplanted as replicated, spaced plants to a field nursery at Blue Creek, Utah. Relative vigor, height, width, total biomass (aboveground dry matter yield), seed weight, and seed number were evaluated between 2009 and 2011. Plant height, width, and total biomass of the F. valesiaca accessions examined were approximately equal to the commercial control, ‘Cascade’ (F. rubra L. subsp. commutata Gaudin; 6x; chewings fescue). Plant vigor and seed weight of F. valesiaca accessions PI 659923, PI 659932, W6 30575, and W6 30588 under semi-arid conditions (~300 mm annual precipitation) were significantly (P < 0.05) greater than ‘Cascade’. Moreover, principal component analysis based on all traits as loading factors indicated that these 12 F. valesiaca accessions were distinct from a majority of the other Festuca accessions examined. These F. valesiaca accessions produced abundant amounts of small seed, and this seed yield was significantly correlated with total aboveground biomass (dry weight; r 2 = 0.84, P < 0.001), plant height (r 2 = 0.58, P < 0.05), and plant vigor (r 2 = 0.83, P < 0.001). Amplified fragment length polymorphism (AFLP) analysis (1,454 polymorphic bands) was used to characterize F. valesiaca relatedness to other economically important Festuca species. The AFLP-based, neighbor-joining analysis differentiated F. valesiaca accessions from US Festuca cultivars examined, except for ‘Durar’ (F. ovina L.; 6x; sheep fescue), to which they had strong genetic affinities. Given their morphological attributes, F. valesiaca PI 659923, W6 30575, PI 659932, and W6 30588 should be considered for use in low-maintenance, semi-arid turf improvement programs in the western US.

Studies onSchismatoglottideae(Araceae) of Borneo XXVI —Schismatoglottis scintillans, a new species with horticultural potential from Sabah, Malaysian Borneo

Schismatoglottis scintillans Scherberich & P. C. Boyce sp. nov., a taxonomic novelty with horticultural potential, is described from Sabah, Malaysian Borneo. Schismatoglottis scintillans belongs to the Calyptrata Group by the presence of hapaxanthic shoot modules, but differs from all species hitherto described for this Group by the combination of refractive variegated leaf blades, a pistillate flower zone extensively adnate to the spathe, a staminate flower zone only half exserted from the lower spathe portion, and a bullet-shaped appendix basally abruptly wider than the adjacent top of the staminate flower zone. The new species is keyed out and illustrated from living plants.

KAJIAN BISNIS SOSIAL PEDAGANG PERANTARA DALAM UPAYA PENGEMBANGAN HORTIKULTURA DI JAWA BARAT

Currently, 90% of Indonesian small scale agribusiness actors arebeing faced to the global market era. They are also facing a fast growthrate of global market and the market segmentation phenomenon whichexpects positive changes and adaptations in the agricultural supply chain.The method that used in this study is case study. The study location wasbeing selected based on its potential of horticulture product.The study showed that currently the traders have changed theirbusiness pattern from a pure business to a business which relies more onthe partnership with the farmers. This phenomenon was being emerged bythe consumer demand which expects the continuity of supply.From the Social Business aspect point of view, generally thetraders do the partnership system because of their needs of continuity ofsupply from the farmers, not based on helping the farmers. The result ofweighting showed the total score placed between 28-32,5 which means itcan be categorized as a semi-social business.Overall, the study showed that currently the changes are happeningin the traders business pattern, thus, it gives hopes to the agriculturaldevelopment in Indonesia especially for horticulture products of smallscale stakeholders.Keywords: Global market, traders, horticulture, social business.

Conventional and In Vitro Propagation of Lechenaultia macrantha (Goodeniaceae)

Lechenaultia macrantha K. Krause (Goodeniaceae) is a species with great horticultural potential that is endemic to the sandy and gravelly soils of central, southwestern Australia. The effectiveness of several conventional and in vitro propagation techniques were assessed. Seeds possessed non-deep physiological dormancy, and a combination of seed nicking and imbibition in 10% (v/v) smoke water for 24 hours resulted in 81% germination after 33 days of incubation. Softwood stem cuttings produced adventitious roots readily without the need for exogenous auxin application. In vitro microcuttings produced shoots in response to cytokinins. However, optimal or suboptimal cytokinin concentrations resulted in hyperhydric shoots, which poses an obstacle to successful micropropagation. The use of auxin was necessary for the initiation of adventitious roots in vitro. This information will assist in the horticultural development of this species.

Phytochemical and antioxidant properties of unconventional leafy vegetables consumed in southern Africa

Indigenous leafy vegetables possess high horticultural potential based on their long utilisation history by local communities across Africa. Phytochemical and antioxidant properties of 50% aqueous methanol and water extracts of three indigenous as well as two commercial leafy vegetables commonly consumed in southern Africa were evaluated. The total extractable phenolic content was highest for Amarathus dubius (5.16±0.12mg GAE/g DW) followed by Cleome gynandra (3.94±0.09mg GAE/g DW). Total flavonoid concentration was highest for A. dubius (3.89±0.28mg CE/g DW) followed by C. gynandra (2.19±0.11mg CE/g DW) and Cucurbita maxima (1.55±0.04mg CE/g DW). No proanthocyanidins were detected in C. maxima and Brassica napus cv Covo whereas low concentrations were recorded in other vegetables. Total saponins were variable across the evaluated extracts, with the highest concentrations recorded for B. napus cv Covo (83.2±16.58mg DE/g DW). Total iridoid content was highest for C. gynandra (9.14±0.20mg HE/g DW). More potent DPPH radical scavenging activities were exhibited by 50% aqueous methanol extracts compared to water extracts. A similar trend was observed in the ferric-reducing antioxidant power assay. The antioxidant activity based on the rate of β-carotene bleaching was higher for water extracts compared to 50% aqueous methanol extracts. The indigenous vegetables evaluated in this study had higher levels of phytochemicals and also exhibited more potent antioxidant activity compared to the commercial varieties. These findings not only suggest the importance of the indigenous vegetables in a healthy diet, but also provide a motivation for exploring their horticultural potential.

Seed Development of Cypripedium debile Rchb. f. in Relation to Asymbiotic Germination

The histological and histochemical changes in developing seeds of Cypripedium debile Rchb. f., a native slipper orchid species with horticultural potential, were investigated. The effects of timing for seed collection, culture media, and cultural conditions were also examined. The optimum germination percentage occurred when mature seeds were collected and sowed on 1/4 Murashige and Skoog basal medium. Besides, the liquid culture promoted germination of mature seeds. This finding is contrary to most other Cypripedium species, which are relatively easy to germinate with immature seeds. Moreover, two notable cytological changes of C. debile were observed. First, the suspensor cell protruded beyond the micropyle opening of the inner seedcoat, making the inner seedcoat not substantial. Second, Nile red staining indicated that the deposition of cuticular material on the seedcoat was fragmentary. It is proposed that the less hydrophobic nature of the seedcoat makes mature seeds of C. debile easier to obtain water and nutrients for germination.

Socio-economic and horticultural potential of Khirni [Manilkara hexandra (Roxb.) Dubard]: a promising underutilized fruit species of India

Manilkara hexandra (Roxb.) Dubard (Khirni) is a socio-economically important fruit and nut species of tribal population of tropical deciduous forests of western and central India. Survey and collection missions were undertaken to study the extent of variability and socio-economic importance of Khirni germplasm in diversity rich areas of Rajasthan, Gujarat, Madhya Pradesh and Maharashtra. A total of 99 accessions of this important tree species were collected from surveyed regions, of which 47 diverse accessions were selected for morphological characterization showing wide range of variation in agro-morphological traits. During the survey, sizable variability in morphological characters and high socio-economic potential of Khirni was recorded. Bark, fresh fruits and extracted seeds have high nutritional and medicinal value. Tree provides substantial livelihood support to local inhabitants as collected fresh fruits from natural populations fetch good price in local markets. Besides livelihood support species, M. hexandra contributes in the nutritional security of the women and children of this area by fulfilling the need of micronutrients and vitamin A. Due to the high market demand of fresh fruits and seeds and without any organized cultivation, pressure on natural wild populations is severely building up since last several decades and therefore, the genetic variability of this species is now facing a great threat and need immediate complementary conservation efforts. Based on higher diversity index and natural population size of this species six in situ conservation sites have been suggested for dynamic conservation in western and central Indian states and 60 accessions have been successfully cryostored.

Micropropagation of Romulea sabulosa Schltr. ex Beg. - A potential ornamental plant

Romulea sabulosa Schltr. ex Beg. is an endemic, attractive and rare flowering species in South Africa with horticultural potential. There is a great need to develop in vitro propagation techniques for this species for the purpose of conservation and commercialization. In this regard, the suitability of the intact seeds and embryos as explants was examined. Excised mature zygotic embryos were selected as the explant source due to a low percentage of seed germination (4.5%). Excised embryos were placed on Murashige and Skoog (MS) media supplemented with different concentrations of kinetin and 2,4-dichlorophenoxyacetic acid (2,4-D). Shoot clusters developed on MS medium supplemented with kinetin without 2,4-D. Multiplication was best on a MS medium supplemented with 2.5μM mTR [6-(3-hydroxybenzylamino)-9-β-d-ribofuranosylpurine]. Shoots were multiplied from selected explants on a MS medium with 23.2μM kinetin and were then used for corm induction experiments. Shoots were placed on MS media supplemented with 3, 6, or 9% sucrose, 5mgl−1 activated charcoal or 23.2μM kinetin. Corm formation was observed for all treatments placed at 10, 15, and 20°C. Corm induction was significantly higher at 10 and 20°C. The heaviest corms were produced at 15°C on a MS medium supplemented with 6% sucrose. In some cases multiple corms formed from a single shoot. The findings of this study will assist in propagating R. sabulosa for commercial and conservation purposes.