Biomass Production Potential Research Articles

Unlocking the potential of Dongting Lake-grown Miscanthus lutarioriparius biomass: A comprehensive quality analysis and bioproduct application study

Miscanthus lutarioriparius grown in Dongting Lake has an annual biomass yield potential of 1 million tons. However, with the shutdown of its previous utilization for paper-making, abandoning this huge amount of biomass has caused serious economic, ecological, and social problems. Constructing an industrial cluster to continuously convert biomass into various bioproducts is a win-win measure to address this dilemma. With the increasing confirmation of the importance of biomass quality affecting the conservation process, fully understanding the biomass characteristics of Dongting Lake-grown M. lutarioriparius is crucial for building a scientific industrial cluster. The present work is designed to explore the variation in biomass quality across the entire Dongting Lake area. Results show that the biomass contented with Cd, Mn, Zn, and Cr has significant geographical differences, with a general trend of Southern Dongting Lake-grown biomass having a higher concentration than that from Eastern and Western Dongting Lake areas. Moreover, significant differences are found in terms of biomass ash content, lignin content, and the degree of polymerization of cellulose (DP). The biomass with low ash content is generally from the entire Eastern Dongting Lake area and the northern part of the Western Dongting Lake area. Virtually all Western Dongting Lake-grown biomass has a low lignin content (approximately 18 %). Regarding the spatial variation of DP, Eastern Dongting Lake-grown biomass has a higher DP (average at 585.33) than that in Southern (575.15) and then Western Dongting Lake (529.16). Based on these quality indicators, the biomass production potentials for bioethanol, biochar, and xylo-oligosaccharide were calculated and visualized. Results show that biomass from almost the entire Western and Eastern Dongting Lake area is suitable for bioethanol and xylo-oligosaccharide production, while biomass from the Southern Dongting Lake area for biochar production. These results provide scientific guidance for the future utilization of Dongting Lake-grown M. lutarioriparius biomass.

Growth and biomass production of five exotic bamboo species in North-western Ethiopia

Forests provide goods and services and the demand for these has increased over the years. To meet this demand, reforestation using fast-growing species is crucial. Bamboo is amongst the fastest-growing plant species. Consequently, the Ministry of Agriculture/East Africa Bamboo Project and the European Union Energy Project/INBAR introduced twenty-three bamboo species to Ethiopia. This study assessed the growth and biomass production potential of five of these exotic bamboo species (Dendrocalamus membranaceus, Dendrocalamus asper, Dendrocalamus hamiltonii, Bambusa vulgaris and Guadua amplexifolia). A randomized complete block design (RCBD) with three replications was used. Nine seedlings of each species were planted per plot using two meters spacing between and within rows. Sprouted culms per clump, culm length, culm diameter at breast height (DBH), average number of nodes and internode length per culm were measured. At age of 3½ years after planting, one clump of each species per plot in each block was uprooted and sorted into different components (rhizome, culm, branch and leaves). The fresh weight of each component was measured and sub-samples were taken to dry to constant weight. One-way ANOVA and then Tukey's test were used to find significantly different group means. The growth performance, in terms of culm production, culm height, internode length and biomass production, of the species was significantly different. D. asper and D. hamiltonii had superior growth and biomass production. We therefore recommend planting these two species in Guangua district and other similar areas where they can enhance the livelihoods of the communities.

A statistical modeling approach based on the small-scale field trial and meteorological data for preliminary prediction of the impact of low temperature on Eucalyptus globulus trees

Eucalyptus trees are important for industrial forestry plantations because of their high potential for biomass production, but their susceptibility to damage at low temperatures restricts their plantation areas. In this study, a 6-year field trial of Eucalyptus globulus was conducted in Tsukuba, Japan, which is the northernmost reach of Eucalyptus plantations, and leaf damage was quantitatively monitored over four of six winters. Leaf photosynthetic quantum yield (QY) levels, an indicator of cold stress-induced damage, fluctuated synchronously with temperature in the winters. We performed a maximum likelihood estimation of the regression model explaining leaf QY using training data subsets for the first 3 years. The resulting model explained QY by the number of days when the daily maximum temperature was below 9.5 °C over approximately the last 7 weeks as an explanatory variable. The correlation coefficient and coefficient of determination of prediction by the model between the predicted and observed values were 0.84 and 0.70, respectively. The model was then used to perform two kinds of simulations. Geographical simulations of potential Eucalyptus plantation areas using global meteorological data from more than 5,000 locations around the world successfully predicted an area that generally agreed with the global Eucalyptus plantation distribution reported previously. Another simulation based on meteorological data of the past 70 years suggested that global warming will increase the potential E. globulus plantation area in Japan approximately 1.5-fold over the next 70 years. These results suggest that the model developed herein would be applicable to preliminary predictions of E. globulus cold damage in the field.

A comparative analysis of growth kinetics, image analysis, and biofuel potential of different algal strains

Due to the global population growth and economic development, energy demand has increased worldwide. Countries take steps to improve their alternative and renewable energy sources. Algae is one of the alternative energy sources and can be used to produce renewable biofuel. In this study, nondestructive, practical, and rapid image processing techniques were applied to determine the algal growth kinetics and biomass potential of four algal strains, including C. minutum, Chlorella sorokiniana, C. vulgaris, and S. obliquus. Laboratory experiments were conducted to determine different aspects of biomass and chlorophyll production of those algal strains. Suitable non-linear growth models, including Logistic, modified Logistic, Gompertz, and modified Gompertz models, were employed to determine the growth pattern of algae. Moreover, the methane potential of harvested biomass was calculated. The algal strains were incubated for 18 days, and the growth kinetics were determined. After the incubation, the biomass was harvested and assessed for its chemical oxygen demand content and biomethane potential. Among the tested strains, C. sorokiniana was the best in biomass productivity (111.97 ± 0.9 mg L−1d−1). The calculated vegetation indices, namely; colorimetric difference, color index vegetation, vegetative, excess green, excess green minus excess red, combination, and brown index values showed a significant correlation with biomass and chlorophyll content. Among the tested growth models, the modified Gompertz shows the best growth pattern. Further, the estimated theoretical CH4 yield was highest for C. minutum (0.98 mL g−1) compared to other tested strains. The present findings suggest that image analysis can be used as an alternative method to study the growth kinetics and biomass production potential of different algae during cultivation in wastewater.

Winter Rye Cover Crop Seeding Rate and Termination Timing Effects on Cover Crop Biomass and Quality

ABSTRACT Winter rye (Secale cereale L.) biomass and chemical composition influences decomposition rates, nutrient cycling and effects on cash crop growth. Management that maintains rye biomass while lowering carbon (C) to nitrogen (N) ratios and concentrations of structural carbohydrates including hemicellulose, cellulose, and lignin could maintain some ecosystem services while accelerating nutrient cycling. Two independent studies examined (1) five rye seeding rates ranging from 0 to 90 kg ha−1 and (2) five termination dates ranging from April 19 to May 31 (585 to 1084 growing degree days) to understand management induced relationships between rye biomass, nutrient concentrations, and composition of hemicellulose, cellulose, and lignin. Rye biomass increased at seeding rates of 90 kg ha−1 as compared to the 22–67 kg ha−1 seeding rates while trends toward higher plant structural components were observed as seeding rate increased. Rye biomass accumulated rapidly beyond 819 (May 13) growing degree days while increasing rye C:N ratio and concentrations of hemicellulose and cellulose. Rye C:N ratio was strongly correlated to rye biomass (R2 = 0.80, p < .001) with a C:N ratio of 25 associated with 2000 kg ha−1 of rye biomass. Changes in rye residue quality were strongly influenced by biomass accumulation with delaying termination most significantly driving biomass changes. Trends toward lower C:N ratios and concentrations of structural carbohydrates were seen at the 22–67 kg ha−1 seeding rates even without differences in biomass suggesting lower seeding rates could cycle nutrients quicker without limiting biomass production potential.

Sixth International Conference on Duckweed Research and Applications Presents Lemnaceae as a Model Plant System in the Genomics and Postgenomics Era.

The 6th International Conference on Duckweed Research and Applications (6th ICDRA) was organized at the Institute of Plant Genetics and Crop Plant Research (IPK) located in Gatersleben, Germany, from 29 May to 1 June 2022. The growing community of duckweed research and application specialists was noted with participants from 21 different countries including an increased share of newly integrated young researchers. The four-day conference focused on diverse aspects of basic and applied research together with practical applications of these tiny aquatic plants that could have an enormous potential for biomass production.

Simulation and Economic Analysis of the Biotechnological Potential of Biomass Production from a Microalgal Consortium

The biomass of microalgae and the compounds that can be obtained from their processing are of great interest for various economic sectors. Chlorophyll from green microalgae has biotechnological applications of great potential in different industrial areas such as food, animal feed, pharmaceuticals, cosmetics, and agriculture. In this paper, the experimental, technical and economic performance of biomass production from a microalgal consortium (Scenedesmus sp., Chlorella sp., Schroderia sp., Spirulina sp., Pediastrum sp., and Chlamydomonas sp.) was investigated in three cultivation systems (phototrophic, heterotrophic and mixotrophic) in combination with the extraction of chlorophyll (a and b) on a large scale using simulation; 1 ha was established as the area for cultivation. In the laboratory-scale experimental stage, biomass and chlorophyll concentrations were determined for 12 days. In the simulation stage, two retention times in the photobioreactor were considered, which generated six case studies for the culture stage. Subsequently, a simulation proposal for the chlorophyll extraction process was evaluated. The highest microalgae biomass concentration was 2.06 g/L in heterotrophic culture, followed by mixotrophic (1.98 g/L). Phototrophic and mixotrophic cultures showed the highest chlorophyll concentrations of 20.5 µg/mL and 13.5 µg/mL, respectively. The simulation shows that higher biomass and chlorophyll production is attained when using the mixotrophic culture with 72 h of retention that we considered to evaluate chlorophyll production (a and b). The operating cost of the entire process is very high; the cultivation stage has the highest operating cost (78%), mainly due to the high energy consumption of the photobioreactors.

Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)

Plant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the "Green Revolution" wheat varieties. However, these gibberellic acid (GA)-insensitive alleles also reduce coleoptile length, biomass production, and yield potential in some environments, triggering the search for alternative GA-sensitive dwarfing genes. Here we report the identification, validation, and characterization of the gene underlying the GA-sensitive dwarfing locus RHT25 in wheat. This gene, designated as PLATZ-A1 (TraesCS6A02G156600), is expressed mainly in the elongating stem and developing spike and encodes a plant-specific AT-rich sequence- and zinc-binding protein (PLATZ). Natural and induced loss-of-function mutations in PLATZ-A1 reduce plant height and its overexpression increases plant height, demonstrating that PLATZ-A1 is the causative gene of RHT25. PLATZ-A1 and RHT1 show a significant genetic interaction on plant height, and their encoded proteins interact with each other in yeast and wheat protoplasts. These results suggest that PLATZ1 can modulate the effect of DELLA on wheat plant height. We identified four natural truncation mutations and one promoter insertion in PLATZ-A1 that are more frequent in modern varieties than in landraces, suggesting positive selection during wheat breeding. These mutations can be used to fine-tune wheat plant height and, in combination with other GA-sensitive dwarfing genes, to replace the GA-insensitive Rht1b alleles and search for grain yield improvements beyond those of the Green Revolution varieties.

Opportunities and Potential for Energy Utilization from Agricultural and Livestock Residues in the Region of Thessaly

In recent years, due to the circular economy, the use of green energy forms, such as biofuels and biogas from anaerobic digestion of fermentable materials (e.g., agricultural and livestock residues) has entered our lives. According to the International Energy Agency it is estimated that the needs in 2040 will be 48% higher than in 2012 so all political decisions have converged on an urgent need for the use of more and more renewable and green energy. Considering the overall economic activity of these sectors in the region of Thessaly, the aim of this study is to highlight the residues from agricultural and livestock activities in the primary sector and calculate the annual biomass production, the methane and biogas potential, the electrical and thermal energy that can be produced from these wastes, as well as the solid residue that can be used to improve the soil of the region. The study was based on data referring to the years 2015 to 2020. The production of livestock and agricultural residues, averaged over the above six-year period in the study area, was estimated at approximately 4.8 × 106 t·yr.−1, with livestock residues accounting for 83% and agricultural residues for 17%. Furthermore, the total residues can produce an average biogas potential of approximately 4.7 × 106 m3·yr.−1, while the amount of electricity that can be produced ranges from 708–1091 GWh·yr.−1, and the corresponding thermal energy from 1112–1577 GWh·yr.−1. As a result of the complete anaerobic digestion process, a solid residue could also be obtained for the improvement of the region’s soil, which translates into a quantity in the range of 4.01 × 104 to 5.10 × 104 t·yr.−1.

Biomass production and carbon storage potential of agroforestry land use systems in high hills of north-western Himalaya: an approach towards natural based climatic solution

Biomass production and carbon storage potential of agroforestry land use systems in high hills of north-western Himalaya: an approach towards natural based climatic solution

Metabolomics reveals arbuscular mycorrhizal fungi-mediated tolerance of walnut to soil drought

BackgroundArbuscular mycorrhizal fungi (AMF) have a positive effect on drought tolerance of plants after establishing reciprocal resymbiosis with roots, while the underlying mechanism is not deciphered. Metabolomics can explain the mechanism of plant response to environmental stress by analyzing the changes of all small molecular weight metabolites. The purpose of this study was to use Ultra High Performance Liquid Chromatography Q Exactive Mass Spectrometer to analyze changes in root metabolites of walnut (Juglans regia) after inoculation with an arbuscular mycorrhizal fungus Diversispora spurca under well-watered (WW) and drought stress (DS).ResultsSixty days of soil drought significantly inhibited root mycorrhizal colonization rate, shoot and root biomass production, and leaf water potential in walnut, while AMF inoculation significantly increased biomass production and leaf water potential, accompanied by a higher increase magnitude under DS versus under WW. A total of 3278 metabolites were identified. Under WW, AMF inoculation up-regulated 172 metabolites and down-regulated 61 metabolites, along with no changes in 1104 metabolites. However, under DS, AMF inoculation up-regulated 49 metabolites and down-regulated 116 metabolites, coupled with no changes in 1172 metabolites. Among them, juglone (a quinone found in walnuts) as the first ranked differential metabolite was up-regulated by AMF under WW but not under DS; 2,3,5-trihydroxy-5–7-dimethoxyflavanone as the first ranked differential metabolite was increased by AMF under DS but not under WW. The KEGG annotation showed a large number of metabolic pathways triggered by AMF, accompanied by different metabolic pathways under WW and DS. Among them, oxidative phosphorylation and phenylalanine metabolism and biosynthesis were triggered by AMF in response to WW and DS, where N-acetyl-L-phenylalanine was induced by AMF to increase under DS, while decreasing under WW.ConclusionThis study provides new insights into the metabolic mechanisms of mycorrhiza-enhanced drought tolerance in walnuts.

Estimation of biomass and carbon storage potential in agroforestry systems of north western Himalayas, India

The estimation of carbon stock in agroforestry is crucial for curbing the global climate crisis. In this study, biomass production and carbon storage potential of the prevalent agroforestry systems along an altitudinal gradient in the Chamba district of Himachal Pradesh was carried out during 2019–2021. Agroforestry practices were categorized into eight land use systems viz., agrisilviculture, agrihorticulture, agrosilvohorticulture, agrohortisilviculture, hortiagriculture, silvopastoral, pastoralsilviculture and hortipastoral systems and were divided into four altitudinal ranges viz., <1000 m above sea level (m asl; Z-I), 1000–1500 m asl (Z-II), 1500–2500 m asl (Z-III) and > 2500 m asl (Z-IV). Results revealed that biomass production and total carbon storage (vegetation + soil) were significantly affected by the land use type along the altitudinal gradient. Among the different agroforestry systems, maximum aboveground biomass (47.48 Mg ha−1) and belowground biomass (12.20 Mg ha−1) were recorded for agrohortisilviculture. Biomass production was positively correlated with altitudes and increased with an increase in altitudes. Soil carbon density was the maximum (35.77 Mg ha−1) under silvopastoral showing a decreasing trend along the altitudinal gradient. The total carbon storage potential was observed the maximum (59.76 Mg ha−1) under silvopastoral system followed by agrohortisilvicuture > agrosilvohorticulture > agrisilviculture > pastoralsilviculture > hortiagriculture > hortipastoral > agrihorticulture. Along the elevation gradient, total carbon stock ranged from 43.99 to 51.13 Mg ha−1 with the maximum carbon stock corresponding to Z-III and the minimum for Z-IV. This study offers a useful approach for selecting different land use systems and vegetation patterns to maximize thecarbon stocks in a cost-effective manner, which will further help to mitigate global warming issues in other fragile Himalayan catchments.

Upscaling cultivation of Saccharina latissima on net or line systems; comparing biomass yields and nutrient extraction potentials

Production of sugar kelp, Saccharina latissima, has the potential of extracting nutrients and carbon from the seawater and returning it to land as a bioresource. The production thereby acts as an emission capture and utilization instrument contributing to mitigation of eutrophication and climate change. To achieve higher biomass yields improving the effects on climate and environment, and the economic feasibility of the production, cultivation techniques need to be optimized. In Denmark so far, S. latissima production yields have been lower than yields documented from Norway and the Faroe Islands. Use of alternative cultivation infrastructure designs with a higher line density per area, and better understanding the effects of annual and seasonal variations in abiotic parameters on growth, could lead the way towards a future higher yield of the S. latissima production. In this study, S. latissima was cultivated in a Danish commercial scale cultivation site for two consecutive seasons comparing the yields of different cultivation techniques: cultivation on nets and a multi-layer single line system, while also testing the possibility of coppicing the blades instead of applying a full harvest. Biomass yields of 5.0 - 6.8 kg FW m-1 cultivation line year-1, and 10.9 – 30.4 kg FW m-2 net structure were achieved. Biomass production, nutrient and carbon extraction potentials up to 91.3 t FW, 110 kg N, 13.1 kg P and 5.1 t C ha-1 were obtained using net cultivation systems and a 1.5 years growth period. Depth of cultivation had a significant effect on yield, but no effect on dry matter, C, N or P contents of S. latissima in a multi-layered line system. Biomass yields from the same system and site varied with a factor of four between years. The use of coppicing enabled multiple harvests of S. latissima, providing however biomass of reduced quality in the second year. This study demonstrates that the production yield of S. latissima can be increased by optimizing cultivation infrastructure, and indicates that net systems, or other cultivation systems with a high line density in the upper water column, can be a means to increase the areal yield of S. latissima.

Initiating guidance values for novel biological stability parameters in drinking water to control regrowth in the distribution system

Nine novel biological stability parameters for drinking water have been developed recently. Here, we report data for these nine parameters in treated water from 34 treatment plants in the Netherlands to deduce guidance values for these parameters. Most parameters did not show a strong correlation with another biological stability parameter in the same sample, demonstrating that most parameters hold different information on the biological stability of drinking water. Furthermore, the novel biological stability parameters in treated water varied considerably between plants and five parameters in treated water were significantly lower for drinking water produced from groundwater than surface water. The maximum biomass concentration (MBC7), cumulative biomass potential (CBP14) from the biomass production potential test (BPP-W) and the total organic carbon concentration in treated water from groundwater were predictive parameters for HPC22 and Aeromonas regrowth in the distribution system. Guidance values of 8.6 ng ATP L−1, 110 d·ng ATP L−1 and 4.1 mg C L−1 were deduced for these parameters, under which the HPC22 and Aeromonas numbers remain at regulatory level. The maximum biomass growth (MBG7) from the BPP-W test, the particulate and/or high molecular organic carbon and the iron accumulation rate in treated water from surface water were predictive parameters for HPC22 and Aeromonas regrowth in the distribution system. Deduced guidance values for these biological stability parameters were 4.5 ng ATP L−1, 47 μg C L−1 and 0.34 mg Fe m−2 day−1, respectively. We conclude from our study that a multiple parameter assessment is required to reliable describe the biological stability of drinking water, that the biological stability of drinking water produced from groundwater is described with other parameters than the biological stability of drinking water produced from surface water, and that guidance values for predictive biological stability parameters were inferred under which HPC22 and Aeromonas regrowth is under control.

Inoculation with Azospirillum brasilense as a strategy to enhance sugarcane biomass production and bioenergy potential

Azospirillum inoculation has gained wide prominence as a strategy for fostering both increased sustainability and enhanced agricultural productivity. Plant growth-promoting bacteria such as Azospirillum improve resource use by plants, but proper management of Azospirillum inoculant application in sugarcane (Saccharum spp.) is key to achieving crop yield potential and improving product quality and economic return for farmers. The present study evaluated the application of different doses of A. brasilense inoculant [0, 5, 10, 15 or 20 × 1010 colony forming units (CFU) ha−1] to sugarcane at the setts/sprouting and tillering stages in two crop seasons at two sites under field conditions. The experiment comprised plant cane and first ratoon at Site A and first and second ratoons at Site B. No effects of inoculation on crop nutrition or the technological quality of the raw material were observed. However, the effects of the inoculation on the biometric parameters and yield of sugarcane varied with the dose. The two highest A. brasilense doses (15 and 20 × 1010 CFU ha−1) increased the stalk population and stalk yield of both plant cane and ratoon sugarcane by approximately 2 stalks m−1 and 20 Mg ha−1, respectively. In addition, the two highest inoculation doses promoted increases in sugar and energy production that were consistent with the increase in stalk population and stalk yield. No clear pattern of the effects of inoculant application time on sugarcane was observed. Overall, A. brasilense inoculation promoted sugarcane productivity when applied at high inoculation doses at the sprouting and tillering stages and has the potential to maximize agronomic and economic benefits to the sugarcane sector.

Tiller Number and Flowering Ability on BC1F1 Progeny of Interspecific Hybridization in Ruzigrass (Urochloa ruziziensis)

The objective of this study focusing on production BC1F1 progeny of interspecific hybridization in ruzigrass (Urochloa ruziziensis) were to evaluate potential of pentaploid BC1F1 hybrid for seed propagation by associating with their tiller number, flowering, and shattering ability. In addition, we evaluate the function of various nitrogen dose on vegetative stages. Eight different genotypes were examined: B. ruziziensis ‘Kennedy’, B. decumbens ‘Basilisk’, pentaploid BC1F1 line derived from B. ruziziensis and Mulato (RM), pentaploid BC1F1 line derived from diploid B. ruziziensis and tetraploid B. ruziziensis (RR), and pentaploid BC1F1 lines derived from B. ruziziensis and B. decumbens (RD 1-4). The experimental plants treated with three level of nitrogen fertilizer (0, 4, and 8 kg/10a). Compared with parental line, RM line had high tiller number along with increasing level of nitrogen. For the flowering and shattering ability, the first lines to flowered and shattered was the pentaploid BC1F1 (RD 1-4), RR, and B. decumbens. Pentaploid BC1F1 lines derived from B. ruziziensis and B. decumbens (RD 1-4) had potential for seed propagation by associating with their flowering and shattering ability, while pentaploid BC1F1 line derived from B. ruziziensis and Mulato (RM) had the potential biomass production and yield ability by associating with its tiller number production.

Ekstrakcija sporednih proizvoda prerade biljnih čajeva uz pretretman gasnom plazmom

Traditional extraction methods involve the use of polar solvents and/or high temperatures. Both are often energy or timeconsuming and require additional purification steps. This compromises the quality of the extracts obtained. Non-thermal plasma pretreatment for extraction is a promising technique that could increase the sustainability of the process while following the principles of hurdle technology. In this work, we investigated the effects of plasma treatment on the aqueous extraction of antioxidant compounds from herbal tea by-products. Stinging nettle, hoary willowherb, and St. John's Wort extracts were treated with plasma at different time intervals. The extracts obtained were analyzed for their antioxidant capacity and total phenolic content. Their potential use as growth media for Ligilactobacillus sp. was also investigated. Hoary willowherb extracts showed the highest total phenolic content and antioxidant activity. While plasma treatment slightly decreased the antioxidant capacity of these extracts, it improved microbial growth. In contrast to hoary willowherb, the antioxidant capacity of stinging nettle extracts' was not affected by plasma treatment. These extracts were also better for bacterial growth. When added to 25% v/v Mrs broth, stinging nettle extracts achieved a growth rate similar to that of pure Mrs broth (~10⁹ CFU/ml), demonstrating their potential for microbial biomass production and food and feed supplementation.

Dry matter yield and nutritive quality of alfalfa (Medicago sativaL.) cultivars grown in sub-humid areas in Ethiopia

Alfalfa (Medicago sativa L.) is one of the most important perennial forage legumes with high nutritional quality. Four alfalfa cultivars were sown in randomized block design with four replications to evaluate their dry matter yield and nutritional quality under rain-fed conditions during 2016–2017 at Masha, southwest Ethiopia. Percentage of plot cover, plant height, leaf to stem ratio, dry matter yield, crude protein yield, and dry matter (DM), ash, crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) were measured. The combined analysis revealed that days to 50% flowering were significantly (P < 0.01) influenced by cultivar and cropping year. Dry matter yield was significantly affected by cultivar and cultivar vs year interaction. Hairy Peruvian scored the highest plot cover percentage followed by Pioneer 00407, Hunter River, and Magna 788. The overall mean for days to 50% flowering indicated that Hunter River reached more lately than others. We observed that leaf-to-stem ratio and plant height were insignificant among the cultivars. Hairy Peruvian gave a higher dry matter yield (15.0 t ha−1) followed by Pioneer 00407 (12.1 t ha−1), Magna 788 (11.5 t ha−1), and Hunter River (10.5 t ha−1). The chemical compositions did not vary significantly (P > 0.05) due to cultivar differences. It could be concluded that, of the tested cultivars, Hairy Peruvian is an appropriate cultivar for biomass production potential under rain-fed conditions for sub-humid areas of southwest Ethiopia.

Natural Capital and Its Services in the Caatinga Phytogeographic Domain

A new millennium has begun, and a new paradigm in exploitation of natural resources to generate products has emerged. In this sense, a model in line with local environmental reality and based on the sustainable development goals (SDG) proposed by the UN is required. The document addresses the suitability of natural capitals in the Caatinga phytogeographic domain (CPD) for generating products, encompassing primary (solar energy and rainfall) and secondary (soil and vegetation) natural resources. These resources have well-defined scarcity (water, soil, and vegetation) and abundance (solar radiation) characteristics. Water is scarce due to uncertain rainfall and frequent droughts. Due to their fragility, soils, when shallow and/or with a low organic matter content, are susceptible to erosion and salinization. In turn, vegetation is scarce due to low potential for biomass production (50% of the area has a biomass production below 2 Mg ha-1. As for solar energy, there is no uncertainty regarding its occurrence in the CPD. Moreover, the region has the highest solar radiation intensities in Brazil, with daily averages ranging from 4.8 to 6.0 Kwh m-2 within a year. Therefore, a new model must be adopted, in which socioeconomic development is based on the potential of local natural resources. In short, abundant resources should be prioritized over scarce ones. Furthermore, exploitation must be democratized and not only in the hands of few privileged individuals

Year-round sustainable biomass production potential of Nannochloris sp. in outdoor raceway pond enabled through strategic photobiological screening.

Microalgae cultivation utilizes the energy of sunlight to reduce carbon dioxide (CO2) for producing renewable energy feedstock. The commercial success of the biological fixation of carbon in a consistent manner depends upon the availability of a robust microalgae strain. In the present work, we report the identification of a novel marine Nannochloris sp. through multiparametric photosynthetic evaluation. Detailed photobiological analysis of this strain has revealed a smaller functional antenna, faster relaxation kinetics of non-photochemical quenching, and a high photosynthetic rate with increasing light and temperatures. Furthermore, laboratory scale growth assessment demonstrated a broad range halotolerance of 10-70 parts per thousand (PPT) and high-temperature tolerance up to 45°C. Such traits led to the translation of biomass productivity potential from the laboratory scale (0.2-3.0 L) to the outdoor 50,000 L raceway pond scale (500-m2) without any pond crashes. The current investigation revealed outdoor single-day peak areal biomass productivity of 43gm-2 d-1 in summer with an annual (March 2019-February 2020) average productivity of 20gm-2 d-1 in seawater. From a sustainability perspective, this is the first report of successful round-the-year (> 347days) multi-season (summer, monsoon, and winter) outdoor cultivation of Nannochlorissp. in broad seawater salinity (1-57 PPT), wide temperature ranges (15-40°C), and in fluctuating light conditions. Concurrently, outdoor cultivation of this strain demonstrated conducive fatty acid distribution, including increased unsaturated fatty acids in winter. This inherent characteristic might play a role in protecting photosynthesis machinery at low temperatures and inhigh light stress. Altogether, our marine Nannochloris sp. showed tremendous potential for commercial scale cultivation to produce biofuels, food ingredients, and asustainable source for vegetarian protein.