Potential Wound Healing Applications Research Articles

Radical formation in skin and preclinical characterization of a novel medical plasma device for dermatology after single application.

Cold atmospheric plasma (CAP) enables painless tissue treatment by producing reactive species including excited molecules and charged particles and is of great interest for medical applications. Medical CAP sources work in contact with air at ambient pressure, resulting in the generation of substantial amounts of reactive oxygen and nitrogen radicals. These radicals have a significant influence on cellular biochemistry, are crucial components of the immune system, and play a central role in wound therapy. CAP has a variety of applications, with a particular emphasis on tissue treatment in dermatology. It eradicates microorganisms by preventing biofilm formation so that wounds can be effectively disinfected and treated antiseptically. Using both in vitro and ex vivo methods, a comprehensive preclinical assessment of a novel battery-operated cold plasma handheld device with a reusable, and autoclavable glass cylinder was performed. The objectives were to evaluate the potential impact of single CAP application on radical formation with and without wound dressing, by directly measuring radicals in skin, to investigate the influence of CAP application on antimicrobial activity and cytotoxicity in vitro, and to assess skin tolerance ex vivo. The direct effect of CAP on the formation of radicals in the skin after plasma application at different levels with and without wound dressing was demonstrated quantitatively for the first time using electron paramagnetic resonance spectroscopy. Free radicals were measured in the skin as a function of the duration of CAP treatment. Furthermore, it was found that an alginate or wound plaster dressing does not significantly inhibit radical formation in skin compared to application without a dressing. In vitro and ex vivo data showed no cytotoxic potential with simultaneous efficacy against bacteria strains and no risk of temperature rise, pH change, skin barrier or DNA damage. These results show a high potential for wound healing applications in vivo.

Topography-Mediated Induction of Epithelial Mesenchymal Transition via Alumina Textiles for Potential Wound Healing Applications.

Substrate topography is vital in determining cell growth and fate of cellular behavior. Although current invitro studies of the underlying cellular signaling pathways mostly rely on their induction by specific growth factors or chemicals, the influence of substrate topography on specific changes in cells has been explored less often. This study explores the impact of substrate topography, specifically the tricot knit microfibrous structure of alumina textiles, on cell behavior, focusing on fibroblasts and keratinocytes for potential wound healing applications. The textiles, studied for the first time as invitro substrates, demonstrated support for keratinocyte adhesion, leading to alterations in cell morphology and the expression of E-cadherin and fibronectin. These topography-induced changes resembled the epithelial-to-mesenchymal transition (EMT), crucial for wound healing, and were specific to keratinocytes and absent in identically treated fibroblasts. Biochemically induced EMT in keratinocytes cultured on flat alumina substrates mirrored the changes seen with alumina textiles alone, suggesting the tricot knit microfibrous topography could serve as an invitro model system to induce EMT-like mechanisms. These results enhance our understanding of how substrate topography influences EMT-related processes in wound healing, paving the way for further evaluation of microfibrous alumina textiles as innovative wound dressings.

Engineering a 3D network hydrogel composite membrane with Chitosan/Pluronic F127/bioactive glass for the topical administration of therapeutic agents

The primary aim of designing a topical drug delivery system is to address localized ailments by administering therapeutic agents directly to superficial areas of the body such as skin and open wounds. This study presents the engineering of a three-dimensional (3D) network hydrogel composite membrane comprising chitosan (CS), Pluronic F127 (Pl), and bioactive glass (BG) for the topical administration. In the presence of CS and Pl, glutaraldehyde forms imine bonds (Schiff base linkages) by reacting its aldehyde groups with the amino groups of the polymer chains. The hydrogel structural and chemical properties were characterized using X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR), revealed its unique composition and molecular structure. Field Emission Scanning electron microscopy (FESEM) was employed to image and investigate the morphology of the hydrogel, confirming its interconnected sheet like morphology with small beads structure. The hydrogels demonstrated excellent water retention ability as 32.93 %, further controlled degradation kinetics rate was systematically evaluated up to 14 days, and optimal swelling behavior makes it suitable for prolonged drug delivery applications. Moreover, hemocompatibility and cyto-compatibility assays yielded less than 1.1 % and above 60 %, confirming the hydrogel safety for blood contact and cell viability. Tube inversion tests confirmed its mechanical integrity, while antibacterial assays revealed its efficacy against pathogenic microorganisms. Co-culture studies demonstrated the capability of developed hydrogel to support cell growth and proliferation. In vitro drug release studies clearly reveal that the hydrogel provides a sustainable delivery system for piperacillin-tazobactam, demonstrating superior characteristics indicative of non-Fickian or anomalous diffusion. Furthermore, the membrane exhibited promising hemostatic properties, suggesting its potential for wound healing applications. Overall, this comprehensive strategy highlights the versatility of the engineered 3D network hydrogel composite membrane as a promising platform for delivering therapeutic agents locally in various biomedical applications.

Inflammatory regulation of squid cartilage gelatin with different molecular weights for treatment of chronic wounds in diabetes

Squid, as a very important economic marine species, accounts for 5 % of the total catch of fish and cephalopods. The waste from the processing process of squid can be used for collagen extraction, which has great application value in the field of biomedical materials. Here, we obtained squid cartilage gelatin (SCG) with different molecular weights by adjusti.ng the reaction conditions and used for the treatment of chronic wounds in diabetes. SCG extracted at low temperatures and short heating times demonstrated a more intact structure, higher molecular weight, and superior gel stability. Based on cell study and transcriptome analysis, SCG with high molecular weight significantly promoted cell adhesion, because it provided more contact sites for cells, whereas small molecules of SCG could directly reduce inflammation. Animal studies have demonstrated that SCG significantly promotes diabetic wound healing as evidenced by reducing inflammation, inducing vascular regeneration, promoting tissue growth, re-epithelialization, collagen deposition and remodeling. This study elucidated the immunoregulatory mechanisms of SCG with different molecular weights, and validated its potential application in chronic wound healing in diabetes.

Assessment of chitosan-PVA hydrogels infused with marine collagen peptides for potential wound healing applications

Ideal wound dressings should show enhanced moisture management at the wound site, antibacterial and physical barrier, and mechanical robustness. Additionally, it should be easy to apply to the wound and be biocompatible and non-toxic. In this study, a linker-free freeze-thaw procedure was used to create an array of chitosan/PVA hydrogels blended with commercially available marine collagen peptides. Marine collagen peptides (CP) are easily available as by-products of the marine food industry and are an inexpensive and novel source of biomaterial in this field. The different weight ratios of chitosan, PVA, and CP influenced the hydrogel properties such as swelling, gel content, evaporation, and mechanical properties. Furthermore, SEM and ATR-FTIR were used to characterize the hydrogels generated under ideal conditions. After 24 h, the optimum hydrogel (chitosan:PVA:CP ratio of 1:5:1) showed a water absorption capacity of up to 900 %, a gel content of 80 %, and a 40 % evaporation rate. The physical interactions between marine collagen peptide and gel-forming components were validated by ATR-FTIR spectra, and the hydrogel kept a sufficient porous structure for potential wound dressing application. To test the mechanical integrity of the hydrogels, compression testing was carried out showing a compressive modulus of up to ∼40 kPa. The addition of marine collagen peptide in the chitosan/PVA hydrogel increased its wettability, antimicrobial capabilities, and hemostatic properties. Furthermore, the hydrogel preparation procedure is simple and does not use toxic chemicals, serving as a model for developing safe and effective hydrogel wound dressing.

Lysozyme-enhanced cellulose nanofiber, chitosan, and graphene oxide multifunctional nanocomposite for potential burn wound healing applications

The demand for advanced biomaterials in medical treatments is rapidly expanding. To address this demand, a nanocomposite of cellulose nanofiber (CNF) with chitosan (Ch) and graphene oxide (GO) was developed for burn wound treatment. The CNF-Ch-GO nanocomposites were characterized and their biological properties were evaluated. Microscopic images showed a uniform distribution of CNF, Ch, and GO with a porous structure. ATR-FTIR and XRD analyses confirmed the chemical structures, while a thermogravimetric study confirmed the stability of CNF-Ch-GO nanocomposite under a N2 atmosphere. The synthesized CNF-Ch-GO nanocomposite exhibited rapid absorption, absorbing 1781.7 ± 53.7 % PBS in 2 min. It demonstrated a Young's modulus of 11.90 ± 0.06 MPa in a hydrated condition, indicating its mechanical stability in water. Furthermore, it displayed excellent biocompatibility and hemocompatibility with 96.23 ± 12.21 % cell viability and 0.21 ± 0.08 % of hemolysis. Additionally, the blood clotting index of CNF-Ch-GO was comparable to that of standard dressing gauze. To enhance antimicrobial efficacy, CNF-Ch-GO was conjugated with lysozyme. This biotic and abiotic conjugation resulted in 92.17 % ± 3.02 % and 94.99 ± 2.1 % eradication of Escherichia coli and Staphylococcus aureus, respectively. The enhanced antimicrobial properties, biocompatibility, and mechanical stability of the superabsorbent CNF-Ch-GO nanocomposite indicate its significant potential for advanced burn wound healing applications.

Quercus infectoria Gall Ethanolic Extract Accelerates Wound Healing through Attenuating Inflammation and Oxidative Injuries in Skin Fibroblasts.

Quercus infectoria Olivier (Fagaceae) nutgall, a traditional Asian medicine, is renowned for its efficacy in treating wounds and skin disorders. Although the gall extract has shown promising results in accelerating wound healing in diabetic animal models, its mechanisms, particularly the effects on redox balance, remain poorly understood. This study aims to investigate the effects and mechanisms of Q. infectoria gall ethanolic extract (QIG) on wound healing in fibroblasts, with a specific emphasis on its modulation of oxidative stress. Hydrogen peroxide (H2O2)-treated L929 cells were used as an in vitro model of oxidation-damaged fibroblasts. QIG exhibited potent antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP) assay values of 305.43 ± 7.48, 508.94 ± 15.12, and 442.08 ± 9.41 µM Trolox equivalents (TE)/µg, respectively. Elevated H2O2 levels significantly reduced L929 cell viability, with a 50% lethal concentration of 1.03 mM. QIG mitigated H2O2-induced cytotoxicity in a dose-dependent manner, showing protective effects in pre-, post-, and co-treatment scenarios. QIG significantly reduced H2O2-induced intracellular reactive oxygen species production and inflammation-related gene expression (p < 0.05). Additionally, at 25 µg/mL, QIG remarkably improved wound closure in H2O2-treated L929 cells by approximately 9.4 times compared with the H2O2 treatment alone (p < 0.05). These findings suggest QIG has potential therapeutic applications in wound healing, mediated through the regulation of oxidative stress and inflammatory response.

MSC Exosomes Containing Valproic Acid Promote Wound Healing by Modulating Inflammation and Angiogenesis.

Chronic wounds that are difficult to heal pose a major challenge for clinicians and researchers. Currently, common treatment methods focus on isolating the wound from the outside world, relying on the tissue at the wound site to grow and heal unaided. Umbilical cord mesenchymal stem cell (MSC) exosomes can promote wound healing by enhancing new blood vessel growth at the wound site. Valproic acid (VPA) reduces the inflammatory response and acts on macrophages to accelerate wound closure. In this study, VPA was loaded into umbilical cord MSC exosomes to form a drug carrier exosome (VPA-EXO) with the aim of investigating the effect of VPA-EXO on wound healing. This study first isolated and obtained umbilical cord MSC exosomes, then added VPA to the exosomes and explored the ability of VPA-EXO to promote the proliferation and migration of human skin fibroblasts (HSFs) and human umbilical vein endothelial cells (HUVECs), as well as the ability to promote the angiogenesis of HUVECs, by using scratch, Transwell, and angiogenesis assays. An in vitro cell model was established and treated with VPA-EXO, and the expression levels of inflammation and pro-angiogenesis-related proteins and genes were examined using Western blot and qRT-PCR. The therapeutic effect of VPA-EXO on promoting wound healing in a whole skin wound model was investigated using image analysis of the wound site, H&E staining, and immunohistochemical staining experiments in a mouse wound model. The in vitro model showed that VPA-EXO effectively promoted the proliferation and migration of human skin fibroblast cells and human umbilical vein endothelial cells; significantly inhibited the expression of MMP-9, IL-1β, IL-8, TNF-α, and PG-E2; and promoted the expression of vascular endothelial growth factors. In the mouse wound model, VPA-EXO reduced inflammation at the wound site, accelerated wound healing, and significantly increased the collagen content of tissue at the wound site. As a complex with dual efficacy in simultaneously promoting tissue regeneration and inhibiting inflammation, VPA-EXO has potential applications in tissue wound healing and vascular regeneration. In future studies, we will further investigate the mechanism of action and application scenarios of drug-loaded exosome complexes in different types of wound healing and vascular regeneration.

Modulation of animal and plant tissue growth with collagen‐starch‐organic molybdenum networks hydrogel biomatrices

AbstractThe development of hydrogel biomatrices with potential to modulate animal and plant tissue growth is ongoing. In this study, molybdenum bio‐metal–organic frameworks (MOFs) (Mo‐bioMOFs) incorporating essential amino acids such as l‐histidine (Mo‐His), l‐phenylalanine (Mo‐Phe), and l‐tryptophan (Mo‐Trp) were encapsulated in semi‐interpenetrating polymer network (semi‐IPN) hydrogels composed of collagen and starch. The structure and properties of these materials show dependence on the amino acid that constitutes the Mo‐bioMOFs. The biomatrices have a semi‐crystalline surface with increased porosity when using Mo‐His; this system also benefits swelling. Increased crosslinking, acceleration in gelation, and mechanical improvement are observed for the system based on Mo‐Phe. Methylene blue release experiments were conducted, demonstrating that matrices including Mo‐bioMOFs exhibit controlled release profiles, indicating highly stable retention of Mo‐bioMOFs in the semi‐IPN matrix. The biomatrices enhance the metabolism and proliferation of fibroblasts and monocytes, with Mo‐Trp reducing the secretion of inflammatory cytokines like TNF‐α. The biomatrices exhibit gradual and slow mass loss when exposed to collagenase and commercial vegetable substrates. Both leaf and root cells of tomato plants (Solanum lycopersicum) show increased metabolism and growth when exposed to Mo‐Phe and Mo‐His. Notably, the biomatrix containing Mo‐Phe promotes the most substantial plant growth and foliage after 30 days. These biomatrices have potential applications in chronic wound healing and agriculture.

Multifunctional emulsion for potential wound-healing applications: A development for co-administration of essential vitamins, silver nanoparticles, and potent active ingredients

This research explores the formulation of novel oil-in-water emulsion designed for the co-delivery of compounds with different properties that jointly represent an exceptional system to apply in skin regeneration, such as essential vitamins A and E, silver nanoparticles, silver sulfadiazine, and lidocaine. First, the preparation method of emulsions was optimized, characterizing their stability over storage time. Emulsions, which were prepared with certain stirring speed and time, presented the lower droplet size and the higher stability for 28 days. Second, the active ingredients were incorporated into the optimized emulsion, and their stability and antimicrobial capacity were evaluated. In addition to being a stable colloidal system, the emulsion with active ingredients demonstrated antimicrobial effects against Pseudomonas aeruginosa and Staphylococcus aureus (both usually present in skin wounds). The antimicrobial properties of silver nanoparticles and the healing-promoting characteristics of vitamins make this combination promising for applications in pharmaceutical and cosmetic formulations. Utilizing natural oils and biocompatible components aligns with sustainable and health-conscious product development trends. The findings of this study hold implications for diverse applications in medicine and cosmetics.

Development of hybrid polyvinylpyrrolidone/carboxymethyl cellulose/collagen incorporated oregano scaffolds via direct ink write printing for potential wound healing applications

Additive manufacturing can develop regenerative scaffolds for wound healing. 3D printing offers meticulous porosity, mechanical integrity, cell adhesion and cost-effectiveness. Herein, we prepared ink composed of carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), collagen, and oregano extract for the fabrication of tissue constructs. The blend was optimized to form a homogeneous ink and rheological characterization demonstrated shear thinning behavior. The scaffolds were printed using Direct Ink Write (DIW) at a flow speed of 4 mm3/s and a layer height of 0.18 mm. The fabricated scaffolds demonstrated an ultimate tensile strength (UTS) and toughness of 730 KPa and 2.72 MJ/m3, respectively. Scanning Electron Microscopy (SEM) revealed an average pore size of 300 ± 30 μm. Fourier transform infrared spectroscopy (FTIR) analysis confirmed that all materials were present. The contact angle of the composite scaffold was 68° ± 1°. Moreover, the scaffolds presented 82 % mass loss (degradation) in phosphate buffer saline (PBS) over 14 days. The composite scaffold exhibited inhibition zones of 9 mm and 12 mm against Staphylococcus aureus and Escherichia coli, respectively. The PVP/CMC/collagen/oregano 3D printed scaffolds exhibited excellent biocompatibility with the mesenchymal stem cells and humman dermal fibroblast cells, confirmed by water-soluble tetrazolium - 8 (WST-8) assay (test conducted for 7 days). The enhanced angiogenic potential of said scaffold was assesed by release of vascular endothelial growth factor followed by further validation through in-vivo CAM assay. Thus, confirming suitability for the potential wound healing application.

Value-Added Nanocellulose Valorized from Fruit Peel Waste for Potential Dermal Wound Healing and Tissue Regenerative Applications

Value-Added Nanocellulose Valorized from Fruit Peel Waste for Potential Dermal Wound Healing and Tissue Regenerative Applications

Development and physiochemical assessment of graphene-bioactive glass-P(3HB-co-4HB) composite scaffold as prospect biomaterial for wound healing

The clinical management of wounds presents a considerable challenge because dressing selection must prioritise the provision of appropriate barrier and the healing properties, consider patient’s compliance factors such as comfort, functionality and practicality. This study primarily aimed to develop a composite scaffold patch for potential application in wound healing. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is a biopolymer that originated from bacteria. It is well-recognised owing to its distinctive mechanical and physical characteristics suitable for biomedical applications. Graphene (G) and bioactive glass (BG) are biocompatible towards humans, and enhanced properties are achievable by adding biopolymer. In this study, composite scaffolds were developed by combining P(3HB-co-4HB) at a distinct proportion of 4HB monomer reinforced with G (3.0 wt.%) and BG (2.5 wt.%) by using solvent casting, resulting in two types of composite scaffolds: P(3HB-co-25%4HB)/G/BG and P(3HB-co-37%4HB)/G/BG. A successful composite scaffold as a unified structure was achieved based on chemical assessments of organic and inorganic elements within the composites. The pure polymer displayed a smooth surface, and the BG and G addition into the composite scaffolds increased surface roughness, forming irregular pores and protuberances. The wettability and hydrophilicity of the composites significantly improved up to 40% in terms of water uptake. An increment in crystallisation temperature diminished the flexibility of the composite’s scaffolds. Evaluation of Presto Blue biocompatibility demonstrated nontoxic behaviour with a dosage of less than 25.00 mg ml−1 of composite scaffold-conditioned media. The L929 fibroblast cells displayed excellent adhesion to both types of composite scaffolds, as evidenced by the increased percentage of cell viability observed throughout 14 d of exposure. These findings demonstrate the importance of optimising each component within the composite scaffolds and their interrelation, paving the way for excellent material properties and enhancing the potential for wound healing applications.

PH‑responsive nanozyme cascade catalysis: A strategy of BiVO4 application for modulation of pathological wound microenvironment

Persistent inflammation and bacterial infection commonly occur during the wound healing process, necessitating urgent development of effective strategies for treating drug-resistant bacterial infections. In this study, bismuth vanadate (BiVO4) was successfully synthesized as an antibacterial agent that promotes wound healing. Through In vitro antibacterial experiments, it was observed that the prepared BiVO4 exhibited excellent performance in catalyzing H2O2 to produce hydroxyl radicals (OH) at a lower concentration (0.2 mg mL−1), resulting in significant antibacterial effects against Gram-negative Extended-Spectrum β-Lactamases-Producing Escherichia coli (ESBL-E. coli) strains. Furthermore, biosafety tests, cell scratch experiments, and ESBL-E. coli infected wound rat model experiments demonstrated high biocompatibility of BiVO4 with a cell survival rate exceeding 85 %. Additionally, BiVO4 promoted the production of vascular endothelial growth factors and fibroblasts migration while contributing to collagen production, effectively facilitating immune reconstruction at the wound site. By integrating peroxidase (POD)-like under acidic conditions (pH 4) and catalase (CAT)-like catalytic activities at under neutral conditions (pH 7), BiVO4 exhibited the ability to activate free radical sterilization and accelerate wound healing by activating O2. Therefore, our findings provide evidence for a dual enzyme regulatory mechanism involving antibacterial properties and promotion of wound tissue reconstruction for potential application in both antibacterial treatment and wound healing.

Sorbremnoids A and B: NLRP3 Inflammasome Inhibitors Discovered from Spatially Restricted Crosstalk of Biosynthetic Pathways.

Crosstalk-oriented chemical evolution of natural products (NPs) is an efficacious strategy for generating novel skeletons through coupling reactions between NP fragments. In this study, two NOD-like receptor protein 3 (NLRP3) inflammasome inhibitors, sorbremnoids A and B (1 and 2), with unprecedented chemical architectures were identified from a fungus Penicillium citrinum. Compounds 1 and 2 exemplify rare instances of hybrid NPs formed via a major facilitator superfamily (MFS)-like enzyme by coupling reactive intermediates from two separate biosynthetic gene clusters (BGCs), pcisor and pci56. Both sorbremnoids A and B are NLRP3 inflammasome inhibitors. Sorbremnoid A demonstrated strong inhibition of IL-1β by directly binding to the NLRP3 protein, inhibiting the assembly and activation of the NLRP3 inflammasome in vitro, with potential application in diabetic refractory wound healing through the suppression of excessive inflammatory responses. This research will inspire the development of anti-NLRP3 inflammasome agents as lead treatments and enhance knowledge pertaining to NPs derived from biosynthetic crosstalk.

Development and characterization of polyethylene oxide and guar gum-based hydrogel; a detailed in-vitro analysis of degradation and drug release kinetics

Herein, we synthesized hydrogel films from crosslinked polyethylene oxide (PEO) and guar gum (GG) which can offer hydrophilicity, antibacterial efficacy, and neovascularization. This study focuses on synthesis and material/biological characterization of rosemary (RM) and citric acid (CA) loaded PEO/GG hydrogel films. Scanning Electron Microscopy images confirmed the porous structure of the developed hydrogel film matrix (PEO/GG) and the dispersion of RM and CA within it. This porous structure promotes moisture adsorption, cell attachment, proliferation, and tissue layer formation. Fourier Transform Infrared Spectroscopy (FTIR) further validated the crosslinking of the PEO/GG matrix, as confirmed by the appearance of C-O-C linkage in the FTIR spectrum. PEO/GG and PEO/GG/RM/CA revealed similar degradation and release kinetics in Dulbecco's Modified Eagle Medium, Simulated Body Fluid, and Phosphate Buffer Saline (degradation of ∼55 % and release of ∼60 % RM in 168 h.). The developed hydrogel film exhibited a zone of inhibition against Escherichia. coli (2 mm) and Staphylococcus. aureus (9 mm), which can be attributed to the presence of RM in the hydrogel film. Furthermore, incorporating CA in the hydrogel film promoted neovascularization, as confirmed by the Chorioallantoic Membrane Assay. The developed RM and CA-loaded PEO/GG-based hydrogel films offered suitable in-vitro properties that may aid in potential wound healing applications.

Development and Investigation of an Innovative 3D Biohybrid Based on Collagen and Silk Sericin Enriched with Flavonoids for Potential Wound Healing Applications.

Skin tissue injuries necessitate particular care due to associated complex healing mechanisms. Current investigations in the domain of tissue engineering and regenerative medicine are focused on obtaining novel scaffolds adapted as potential delivery systems to restore lost tissue functions and properties. In this study, we describe the fabrication and evaluation of a novel 3D scaffold structure based on collagen and silk sericin (CollSS) enriched with microcapsules containing natural compounds, curcumin (C), and/or quercetin (Q). These 3D composites were characterized by FT-IR spectroscopy, water uptake, in vitro collagenase degradation, and SEM microscopy. Furthermore, they were biologically evaluated in terms of biocompatibility, cell adhesion, anti-inflammatory, and antioxidant properties. All tested materials indicated an overall suitable biocompatibility, with the best results obtained for the one containing both flavonoids. This study suggests the cumulative beneficial effect of C and Q, encapsulated in the same composite, as a potential non-invasive therapeutic strategy for skin tissue regeneration in patients suffering from chronic wounds.

The effect of MgO nanoparticle on PVA/PEG-based membranes for potential application in wound healing

The interest in wound dressings increased ten years ago. Wound care practitioners can now use interactive/bioactive dressings and tissue-engineered skin substitutes. Several bandages can heal burns, but none can treat all chronic wounds. This study formulates a composite material from 70% polyvinyl alcohol (PVA) and 30% polyethylene glycol (PEG) with 0.2, 0.4, and 0.6 wt% magnesium oxide nanoparticles. This study aims to create a biodegradable wound dressing. A Fourier Transform Infrared (FTIR) study shows that PVA, PEG, and MgO create hydrogen bonding interactions. Hydrophilic characteristics are shown by the polymeric blend’s 56.289° contact angle. MgO also lowers the contact angle, making the film more hydrophilic. Hydrophilicity improves film biocompatibility, live cell adhesion, wound healing, and wound dressing degradability. Differential Scanning Calorimeter (DSC) findings suggest the PVA/PEG combination melted at 53.16 °C. However, adding different weight fractions of MgO nanoparticles increased the nanocomposite’s melting temperature (Tm). These nanoparticles improve the film’s thermal stability, increasing Tm. In addition, MgO nanoparticles in the polymer blend increased tensile strength and elastic modulus. This is due to the blend’s strong adherence to the reinforcing phase and MgO nanoparticles’ ceramic material which has a great mechanical strength. The combination of 70% PVA + 30% PEG exhibited good antibacterial spatially at 0.2% MgO, according to antibacterial test results.

Preparation of β-cyclodextrin derivatives/metamizole inclusion complex nanofibers: Characterization, drug release, and wound scratch assay

Nanofibrous wound dressings fabricated by electrospinning have attracted significant research interest as they enable the controlled release of active substances at the wound site. The development of electrospun nanofibers with anti-inflammatory drugs, which demonstrate great potential for wound healing applications, is one of the main focuses of present research efforts. In this study, nanofibrous webs of 2-hydroxypropyl-β-cyclodextrin (HPβCD) and methyl-β-cyclodextrin (MβCD) loaded with metamizole (MMS) were fabricated by electrospinning. The prepared nanofibers were characterized by field-emission scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, thermogravimetric/differential thermal analysis, 1H nuclear magnetic resonance spectroscopy, ultraviolet diffuse reflectance spectroscopy, and photoluminescence analysis. The interactions of HPβCD and MβCD with MMS in aqueous solutions were studied using absorption and fluorescence spectroscopy, and the 1:1 MMS/CD inclusion complexes were theoretically analyzed at the PM3 level of theory. Furthermore, the drug release, biocompatibility, and wound scratch assay of the nanofibers were evaluated. In vitro drug release analysis revealed controllable release of MMS from the nanofibers. The CD nanofibers loaded with MMS showed good biocompatibility with skin fibroblasts. An in vitro wound scratch assay indicated that the nanofibers promoted fast wound closure with a rate of 98.66 ± 3.9%. In summary, the prepared nanofibrous webs are promising candidates for wound-dressing applications.

Fabrication, Characterization of Curcumin Loaded Alginate Chitosan for Potential Wound Healing Applications

The most employed scaffolds in tissue engineering are alginate and chitosan due to their properties like biodegradability, compatibility, and structural similarity to that of the ECM. Curcumin, together with scaffolds such as alginate and chitosan, can improve wound healing properties by tissue repair and regeneration. This study aims to load curcumin into alginate chitosan scaffolds and to analyze their potential wound healing properties by characterization and checking their biocompatibility. Curcumin was loaded into the alginate-chitosan scaffold. It was then characterized using Fourier Transform-InfraRed (FT-IR) spectroscopy and Scanning Electron Microscopy (SEM). Annexin V PI apoptotic assay and Hemolytic assay were done to screen its biocompatibility. FT-IR has strong absorption bands at 3237, 2359, 1597, 1406, 1025, and 947 cm−1. SEM analysis of the curcumin-loaded alginate-chitosan scaffold showed the dispersed curcumin on the surface of the porous scaffold. Our results suggest that the curcumin-loaded alginate-chitosan scaffold possesses greater biocompatibility towards peripheral blood mononuclear cells (PBMC) which was confirmed by Annexin V - PI assay and hemolytic assay. Curcumin loaded onto an alginate-chitosan scaffold is reported to be biocompatible using flow cytometry and hemolytic assay. However a more detailed study must be done before using it for potential wound healing applications.