Preparation of β-cyclodextrin derivatives/metamizole inclusion complex nanofibers: Characterization, drug release, and wound scratch assay

Abstract

Nanofibrous wound dressings fabricated by electrospinning have attracted significant research interest as they enable the controlled release of active substances at the wound site. The development of electrospun nanofibers with anti-inflammatory drugs, which demonstrate great potential for wound healing applications, is one of the main focuses of present research efforts. In this study, nanofibrous webs of 2-hydroxypropyl-β-cyclodextrin (HPβCD) and methyl-β-cyclodextrin (MβCD) loaded with metamizole (MMS) were fabricated by electrospinning. The prepared nanofibers were characterized by field-emission scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, thermogravimetric/differential thermal analysis, 1H nuclear magnetic resonance spectroscopy, ultraviolet diffuse reflectance spectroscopy, and photoluminescence analysis. The interactions of HPβCD and MβCD with MMS in aqueous solutions were studied using absorption and fluorescence spectroscopy, and the 1:1 MMS/CD inclusion complexes were theoretically analyzed at the PM3 level of theory. Furthermore, the drug release, biocompatibility, and wound scratch assay of the nanofibers were evaluated. In vitro drug release analysis revealed controllable release of MMS from the nanofibers. The CD nanofibers loaded with MMS showed good biocompatibility with skin fibroblasts. An in vitro wound scratch assay indicated that the nanofibers promoted fast wound closure with a rate of 98.66 ± 3.9%. In summary, the prepared nanofibrous webs are promising candidates for wound-dressing applications.