Potential Adverse Health Effects Research Articles

Exposure Assessment Techniques Applied to the Highly Censored Deepwater Horizon Gulf Oil Spill Personal Measurements.

The GuLF Long-term Follow-up Study (GuLF STUDY) is investigating potential adverse health effects of workers involved in the Deepwater Horizon (DWH) oil spill response and cleanup (OSRC). Over 93% of the 160 000 personal air measurements taken on OSRC workers were below the limit of detection (LOD), as reported by the analytic labs. At this high level of censoring, our ability to develop exposure estimates was limited. The primary objective here was to reduce the number of measurements below the labs' reported LODs to reflect the analytic methods' true LODs, thereby facilitating the use of a relatively unbiased and precise Bayesian method to develop exposure estimates for study exposure groups (EGs). The estimates informed a job-exposure matrix to characterize exposure of study participants. A second objective was to develop descriptive statistics for relevant EGs that did not meet the Bayesian criteria of sample size ≥5 and censoring ≤80% to achieve the aforementioned level of bias and precision. One of the analytic labs recalculated the measurements using the analytic method's LOD; the second lab provided raw analytical data, allowing us to recalculate the data values that fell between the originally reported LOD and the analytical method's LOD. We developed rules for developing Bayesian estimates for EGs with >80% censoring. The remaining EGs were 100% censored. An order-based statistical method (OBSM) was developed to estimate exposures that considered the number of measurements, geometric standard deviation, and average LOD of the censored samples for N ≥ 20. For N < 20, substitution of ½ of the LOD was assigned. Recalculation of the measurements lowered overall censoring from 93.2 to 60.5% and of the THC measurements, from 83.1 to 11.2%. A total of 71% of the EGs met the ≤15% relative bias and <65% imprecision goal. Another 15% had censoring >80% but enough non-censored measurements to apply Bayesian methods. We used the OBSM for 3% of the estimates and the simple substitution method for 11%. The methods presented here substantially reduced the degree of censoring in the dataset and increased the number of EGs meeting our Bayesian method's desired performance goal. The OBSM allowed for a systematic and consistent approach impacting only the lowest of the exposure estimates. This approach should be considered when dealing with highly censored datasets.

Probabilistic multi-pathway human health risk assessment due to heavy metal(loid)s in a traditional gold mining area in Ecuador

Mining operations are important causes of environmental pollution in developing countries where mining waste management is not adequate. Consequently, heavy metal(loid)s are easily released into the environment, being a potential risk to human health. This study carries out a Bayesian probabilistic human health risk assessment, related to multi-pathway exposure to heavy metal(loid)s in a gold mining area in Southern Ecuador. Concentrations of As, Cd, Cr, Cu, Ni, Pb, and Zn in tap water, surface water, and soil samples, were analyzed to assess the potential adverse human health effects based on the Hazard Index (HI) and Total cancer risk (TCR). Adults and children residents were surveyed to adjust their exposure parameters to the site-specific conditions. Exposure to heavy metal(loid)s resulted in unacceptable risk levels for human health in the two age groups, both carcinogenic (TCR > 1 × 10−5) and non-carcinogenic (HI > 1) through ingestion of tap water and incidental ingestion of surface water. Sensitivity analysis showed that As concentration in waters and exposure frequency were the main contributors to risk outcome. Exposure to soil via accidental ingestion and dermal contact was below the safety limit, not posing a risk to human health. These findings can provide a baseline for the environmental management of the mining area and indicate the need for further research on As pollution in water and its implications on the health of the inhabitants of mining communities.

Heavy metals levels and associated health risk assessment of Euphorbia granulata Forssk.

Medicinal plants are considered as the safest approach to treat various ailments. However, prolonged consumption of plants containing high levels of heavy metals (HMs) can lead to deleterious consequences. Euphorbia granulata (EG) is therapeutically used in Pakistan and many parts of the world. This study assessed EG from three districts of Khyber Pakhun Khwa, Pakistan, for the levels of seven HMs (Pb, Cd, Cr, Ni, Ag, Hg, and As) and the associated health risks. Non-carcinogenic health risk associated with HMs was assessed from hazard quotients (THQ) and hazard index (HI). THQ values of Hg for Peshawar (1.566) and Mardan (1.897) were above the safe recommended limits (< 1) of USEPA. Overall more than 80% of THQ was contributed by the Hg alone. Total cancer risk (TCR) was calculated using incremental lifetime cancer risk (ILCR). The hazard index (HI) displayed by Peshawar (1.87) and Mardan (2.7) samples indicated the potential adverse health effects due to EG. Furthermore, the TCR for Peshawar (2.2 × 10-4), Mardam (2.3 × 10-4), and Swat (1.4 × 10-4) was also more than US-EPA recommended threshold value (1 × 10-4). Our results unveiled the possibility of carcinogenic health risks with Pb, Cd, Cr, Ni, and As to EG consumers. Due to potential non-carcinogenic and carcinogenic health risk, chronic use of E. granulata is not recommended, particularly from HMs contaminated sites.

Polybrominated diphenyl ethers and the multi-element profile of house dust in Croatia: Indoor sources, influencing factors of their accumulation and health risk assessment for humans

Given the large amount of time people spend indoors today, human exposure to indoor contaminants causes increasing public health concerns. The present study reports for the first time the levels of 7 polybrominated diphenyl ether (PBDE) congeners, and 18 trace elements measured in dust samples collected in 68 households from Zagreb, Croatia. Based on the obtained data from dust analysis and the questionnaire on the house characteristics and habits of the residents, we aimed to assess the possible indoor sources of PBDEs/elements, and the associated health risks.Mass concentrations of ΣPBDE ranged from 0.16 and 200.09 ng g−1 dust (median 4.19 ng g−1 dust). The most frequently detected congeners were BDE-99 and BDE-183 found in >88% of samples, while for trace elements, Al, Fe, Zn, Mn and Cu were found at the highest concentrations (enumerated in the descending order). The regression analysis indicated that renovation, number of residents and hours spent using electronic devices are significant predictors for determining PBDE dust concentrations, while the house age, and the house area were identified as the most important contributors for most trace elements. Our health risk assessment considering dust ingestion and dermal absorption of analyzed dust indicated that no adverse health effects are expected in toddlers and adults from exposure to PBDEs or trace elements in house dust. However, calculating the worst case exposure scenario based on the maximum measured concentrations and high dust intake rates, it was estimated that there is a risk of potential adverse health effects for Co (HI > 1). Even though the cases of high exposure to toxic elements from dust are sporadic, and not common among the general population, this exposure scenario should be included whenever assessing the background exposure of children.

Hazard prioritisation of substances in printing inks and adhesives applied to plastic food packaging

ABSTRACT Thousands of intentionally added substances can be used in printing inks and adhesives applied to plastic food packaging. Some of them can be transferred to foodstuffs through a phenomenon called migration, arising concerns on the potential adverse health effects derived from the exposure to chemicals that have not yet been assessed for their risks to humans. The large number of the substances concerned and the lack of prioritisation strategies hamper the work of control authorities, since it is not clear which substances should be monitored as first priority. In this study, a hazard prioritisation strategy is proposed. An inventory listing more than 6,000 substances used in inks and adhesives applied to plastic food packaging was compiled and filtered using several exclusion criteria aimed to set apart those substances for which there is no apparent need for further evaluation or because fall into one of the exclusion categories of the Threshold of Toxicological Concern (TTC) approach. Additionally, substances with a molecular weight >1,000 Da were removed. Approximately 2,300 substances were retained, for which a comprehensive hazard profiling was conducted based on the general scheme for the application of the TTC approach. First, structural alerts for genotoxic and non-genotoxic carcinogenicity were investigated. If a substance was neither genotoxic nor belonging to the chemical classes of organophosphates and carbamates, the Cramer classification was used. Furthermore, the substances were searched for their presence in three so-called ‘Substances of Concern’ lists and RASFF notifications. Groups of high, medium and low priority substances were established, resulting in 1,660 substances classified as high and medium priority. A panel of five experts evaluated these substances with respect to their relevance for further risk evaluations. By applying this hazard prioritisation strategy, 696 substances were identified as ‘Very High Priority Substances’ (VHPS) for which further assessments should be performed.

Potential adverse health consequences of climate change related to rheumatic diseases

There is a growing body of literature regarding the health implications of climate change for the general population, but a scarcity of data specific to patients with rheumatic diseases. Human health will be affected by extreme weather events, heat waves, increased wildfires and a spread in the distribution of vector-borne diseases. Patients with underlying pulmonary, cardiac and renal disease are at high risk for adverse health outcomes related to these events. Many patients with rheumatic diseases share these comorbidities, which would presumably also put them at high risk for poor outcomes. There is research on ambient air pollution that indicates disease flares in patients with systemic lupus erythematosus are linked to elevated particulate matter. These patients are probably also at risk from increased particulate matter emanating from major wildfires. More research is needed on the specific adverse health impacts of climate change on patients with rheumatic diseases in order to guide policy decisions to mitigate the adverse impacts.

Comparative study of two isothiazolinone biocides, 1,2-benzisothiazolin-3-one (BIT) and 4,5-dichloro-2-n-octyl-isothiazolin-3-one (DCOIT), on barrier function and mitochondrial bioenergetics using murine brain endothelial cell line (bEND.3)

ABSTRACT Isothiazolinone (IT) biocides are potent antibacterial substances used as preservatives and disinfectants. These biocides exert differing biocidal effects and display environmental stability based upon chemical structure. In agreement with our recent study reporting that 2-n-octyl-4-isothiazolin-3-one (OIT) induced dysfunction of the blood-brain barrier (BBB), the potential adverse health effects of two IT biocides 1,2-benzisothiazolin-3-one (BIT) and 4,5-dichloro-2-n-octyl-isothiazolin-3-one (DCOIT) were compared using brain endothelial cells (ECs) derived from murine brain endothelial cell line (bEND.3). BIT possesses an unchlorinated IT ring structure and used as a preservative in cleaning products. DCOIT contains a chlorinated IT ring structure and employed as an antifouling agent in paints. Data demonstrated that DCOIT altered cellular metabolism at a lower concentration than BIT. Both BIT and DCOIT increased reactive oxygen species (ROS) generation at the mitochondrial and cellular levels. However, the effect of DCOIT on glutathione (GSH) levels appeared to be greater than BIT. While mitochondrial membrane potential (MMP) was decreased in both BIT- and DCOIT-exposed cells, direct disturbance in mitochondrial bioenergetic flux was only observed in BIT-treated ECs. Taken together, IT biocides produced toxicity in brain EC and barrier dysfunction, but at different concentration ranges suggesting distinct differing mechanisms related to chemical structure.

Relationship of Physical Activity at Older Age with Biomarkers of Oxidative Stress: A Large, Population-based Cohort Study.

No population-based cohort study on the associations of physical activity with biomarkers of oxidative stress has been performed so far. The total thiol groups of serum proteins (TTP), which can be considered as a proxy biomarker for the antioxidant defense capacity of cells and the derivatives of reactive oxygen metabolites (D-ROM) serum concentration, which is mainly a biomarker of lipid peroxidation, were measured in 2572 participants of a population-based cohort study of older adults (age range, 57-83 yr), of whom 2068 had repeated measurements 3 yr later. Physical activity was assessed by a questionnaire specifically designed for the elderly. In multivariable linear regression models, total physical activity was statistically significantly, inversely associated with both D-ROM concentrations measured at baseline and their 3-yr change. With respect to TTP, a nonsignificant, positive association with total physical activity was observed in the cross-sectional analysis, which was statistically significant in obese study participants, and a statistically significant interaction between physical activity and obesity was detected. However, no longitudinal association between total physical activity and changes in TTP levels was observed. The type of physical activity (sports, leisure time, or household activity) did not have a strong effect on the results. This first population-based cohort study suggests that regular physical activity at older age could reduce oxidative stress. With the multifold potential adverse health consequences of chronically increased, systemic oxidative stress in mind, physical activity should be intensively promoted for all older adults as a measure to prevent age-related diseases.

Human health risk of mercury in street dust: A case study of children in the vicinity of compact fluorescence lamp factory, Hanoi, Vietnam.

Urban dust has been contaminated with toxic heavy metals from various diffused sources. On August 28, 2019, the compact fluorescence lamp (CFL) factory that generates light for Rang Dong Light Source and Vacuum Flask in Hanoi City in Vietnam was accidentally fired. The assessment for Hg contamination and its human health impact 2 months after the incident was conducted in autumn 2019. The heavy Hg pollution is still found near CFL. Additionally, localized Hg pollution by air and wind direction was identified at sites in the northeast direction from CFL. Namely, in the area located downstream of the southwest monsoon wind, Hg had a trend higher than other directions. The hazard quotient (HQ) and the hazard index (HI) were evaluated by four routes of Hg exposure pathways. The HI values for children and adults in the most polluted site (Site 15) were 0.968 and 0.468, respectively. This meant that children who lived around this site and commuted to schools were the most at risk regarding the potential adverse health effects of Hg in street dust around the polluted areas. This study also showed that Hg levels were affected by wind direction and that the higher Hg levels in the most polluted sites resulted from the wind flow to the sites. This study revealed that street dust may be a useful tool for the assessment of human and environmental health.

Effect of acid treated HY zeolites in adsorption of mesosulfuron-methyl.

Pollution of surface water and groundwater by bulky molecules such as pesticides has been recognized as a major problem in many countries due to their persistence in aquatic environment and potential adverse health effects. The main purpose of this study is the development of a capable adsorbent to remove these bulky molecules from wastewater such as the pesticide Mesosulfuron-Methyl (MM) by reducing the diffusion path, to overcome the problems of diffusional limitations on microporous adsorbents. The adsorption of mesosulfuron-methyl (MM) from aqueous solution is curried out using treated acid HY zeolite. Batch sorption equilibrium and kinetic experiments are conducted to evaluate the efficiency of these materials. Parent zeolites and their derivatives have been characterized by nitrogen adsorption-desorption, pyridine chemisorption followed by infrared spectroscopy and X-ray fluorescence. The acid treatment leads to an increase in the specific surface from 691 to 853 m2 g- 1 for HY(30) and from 631 to 806 m2 g- 1 for the HY(16.6) zeolites. It also leads to a reduction in Lewis acidity from 74 to 25 µmol g- 1 and from 135 to 31 µmol g- 1 for HY(30) and HY(16.6) zeolites respectively, and increases the adsorbent-adsorbate interaction. The adsorption capacity increased from 83 to 99 % after acid treatment. The equilibrium adsorption time is decreased from 15h to 10min for the HY(30)_A and from 20h to 20min for the HY(16.6)_A for an initial concentration of 20mg L- 1. The adsorption capacity depends on the pH solution, and the neutral form of the MM is more easily adsorbed into zeolite than the dissociated form via the framework bridged oxygen atoms. For all the samples, the pseudo-second-order kinetic model fits very well with the experimental data. In the case of the modified zeolites, the approaching equilibrium factor R w decreases from 0.08183 to 0.00008 when the Lewis acid sites decrease; indicating that the equilibrium is reached more quickly. S-shape adsorption isotherms indicates that cooperative adsorption phenomena. Nevertheless, the shape of acid treated zeolites evolves to an L type indicating a significant enhancement of the adsorbent - adsorbate interactions inducing better adsorption efficiency. Mesosulfuron-methyl adsorption has been successfully enhanced after acid treatments of zeolites HY.

A conceptual model of hydrogeological function and perchlorate transfer in the unconfined Champagne Chalk aquifer (NE France)

Abstract Groundwater of the unconfined Chalk aquifer in Champagne-Ardenne (NE France) is contaminated by perchlorate ( ClO 4 − ), a persistent water-soluble anion. The Chalk aquifer is a crucial water resource of the region, with complex hydraulic properties. The presence of ClO 4 − is of concern due to its potential adverse human health effects. In France, three sources of ClO 4 − contamination are suspected: industrial, military and agricultural. Both a comprehensive understanding of hydrogeological characteristics of the aquifer, and a sound knowledge of sources and behaviour of ClO 4 − in groundwater are required to allow the sustainable use of this groundwater resource. From data acquired during hydrogeological, geochemical, isotopic ( ClO 4 − ) and groundwater age (CFCs and SF 6 ) studies, and historical investigations in a study area located east of Reims, a conceptual model of Chalk aquifer function and ClO 4 − transfer has been established. High spatio-temporal heterogeneities in the unconfined Chalk aquifer are discussed. Different correlations between ClO 4 − , major ions and groundwater-level fluctuations are shown and interpreted, highlighting main factors governing the Chalk groundwater geochemistry and ClO 4 − transfer mechanisms, including water-level fluctuation, groundwater residence time, thickness of the unsaturated zone, superficial formations, distribution of fissure networks, aquifer–river relationships, origin and location of ClO 4 − in soil and human activity.

Continuous Long-Term Exposure to Low Concentrations of MWCNTs Induces an Epithelial-Mesenchymal Transition in BEAS-2B Cells.

In the field of nanotechnology, the use of multi-walled carbon nanotubes (MWCNTs) is growing. Pulmonary exposure during their production, use, and handling is raising concerns about their potential adverse health effects. The purpose of this study is to assess how the physical characteristics of MWCNTs, such as diameter and/or length, can play a role in cellular toxicity. Our experimental design is based on the treatment of human bronchial epithelial cells (BEAS-2B) for six weeks with low concentrations (0.125–1 µg/cm2) of MWCNTs having opposite characteristics: NM-403 and Mitsui-7. Following treatment with both MWCNTs, we observed an increase in mitotic abnormalities and micronucleus-positive cells. The cytotoxic effect was delayed in cells treated with NM-403 compared to Mitsui-7. After 4–6 weeks of treatment, a clear cellular morphological change from epithelial to fibroblast-like phenotype was noted, together with a change in the cell population composition. BEAS-2B cells underwent a conversion from the epithelial to mesenchymal state as we observed a decrease in the epithelial marker E-cadherin and an increased expression of mesenchymal markers N-cadherin, Vimentin, and Fibronectin. After four weeks of recovery, we showed that the induced epithelial-mesenchymal transition is reversible, and that the degree of reversibility depends on the MWCNT.

Influence of perceived stress on prenatal depression in Surinamese women enrolled in the CCREOH study

BackgroundPrenatal depression may have adverse health effects on mothers and their offspring. Perceived stress is an important risk factor for depression during pregnancy. Studies have shown that both perceived stress and depression may negatively influence birth outcomes. While 20% of pregnancies in Suriname, a middle-income Caribbean country located in northern South America, results in adverse birth outcomes, data on prenatal depression and its risk factors are lacking. This study aimed to assess the influence of perceived stress on depression during pregnancy in Surinamese women.MethodsSurvey data were used from 1143 pregnant women who participated in the Caribbean Consortium for Research in Environmental and Occupational Health-MeKiTamara prospective cohort study that addresses the impact of chemical and non-chemical environmental exposures in mother/child dyads in Suriname. The Edinburgh Depression Scale and Cohen Perceived Stress Scale were used to screen for probable depression (cut-off ≥ 12) and high stress (cut-off ≥ 20), respectively. The association between perceived stress and depression was examined using bivariate and multiple logistic regression analyses, adjusted for social support (including resilience) and maternal demographics.ResultsThe prevalence of high perceived stress during the first two trimesters and the third trimester were 27.2% and 24.7% respectively. 22.4% of the participants had probable depression during first or second trimester and 17.6% during the third trimester. Women experiencing high stress levels during the first two trimesters had 1.92 increased odds (95% CI 1.18–3.11, p = 0.008) of having probable depression during the third trimester of pregnancy than those with low stress levels. Pregnant women with low individual resilience during early pregnancy (52.1%) had 1.65 (95% CI 1.03–2.63, p = 0.038) increased odds of having probable depression during later stages of pregnancy compared to those with high individual resilience. Low educational level (p = 0.004) and age of the mother (20–34 years) (p = 0.023) were significantly associated with probable depression during the third trimester.ConclusionsEarly detection and management of stress and depression during pregnancy are important. Health education programs, targeting the reduction of stress during pregnancy, may help to reduce depression and its potential adverse health effects on the mother and child.

Acute cardiovascular effects of controlled exposure to dilute Petrodiesel and biodiesel exhaust in healthy volunteers: a crossover study

BackgroundAir pollution derived from combustion is associated with considerable cardiorespiratory morbidity and mortality in addition to environmental effects. Replacing petrodiesel with biodiesel may have ecological benefits, but impacts on human health remain unquantified.The objective was to compare acute cardiovascular effects of blended and pure biodiesel exhaust exposure against known adverse effects of petrodiesel exhaust (PDE) exposure in human subjects.In two randomized controlled double-blind crossover studies, healthy volunteers were exposed to PDE or biodiesel exhaust for one hour. In study one, 16 subjects were exposed, on separate occasions, to PDE and 30% rapeseed methyl ester biodiesel blend (RME30) exhaust, aiming at PM10 300 μg/m3. In study two, 19 male subjects were separately exposed to PDE and exhaust from a 100% RME fuel (RME100) using similar engine load and exhaust dilution. Generated exhaust was analyzed for physicochemical composition and oxidative potential. Following exposure, vascular endothelial function was assessed using forearm venous occlusion plethysmography and ex vivo thrombus formation was assessed using a Badimon chamber model of acute arterial injury. Biomarkers of inflammation, platelet activation and fibrinolysis were measured in the blood.ResultsIn study 1, PDE and RME30 exposures were at comparable PM levels (314 ± 27 μg/m3; (PM10 ± SD) and 309 ± 30 μg/m3 respectively), whereas in study 2, the PDE exposure concentrations remained similar (310 ± 34 μg/m3), but RME100 levels were lower in PM (165 ± 16 μg/m3) and PAHs, but higher in particle number concentration. Compared to PDE, PM from RME had less oxidative potential. Forearm infusion of the vasodilators acetylcholine, bradykinin, sodium nitroprusside and verapamil resulted in dose-dependent increases in blood flow after all exposures. Vasodilatation and ex vivo thrombus formation were similar following exposure to exhaust from petrodiesel and the two biodiesel formulations (RME30 and RME100). There were no significant differences in blood biomarkers or exhaled nitric oxide levels between exposures.ConclusionsDespite differences in PM composition and particle reactivity, controlled exposure to biodiesel exhaust was associated with similar cardiovascular effects to PDE. We suggest that the potential adverse health effects of biodiesel fuel emissions should be taken into account when evaluating future fuel policies.Trial registrationClinicalTrials.gov, NCT01337882/NCT01883466. Date of first enrollment March 11, 2011, registered April 19, 2011, i.e. retrospectively registered.

Metal Contamination in Macrobrachium rosenbergii from Sarawak River, Malaysia and its Health Risk to Human

An assessment of heavy metal concentration in wild-captured Macrobrachium rosenbergii (giant freshwater prawns) from their natural habitat is important since it is susceptible to environmental pollution due to the rapid development and human activities along the Sarawak River. This study aimed to estimate the heavy metal contamination in muscles, exoskeletons and gills of the M. rosenbergii and to estimate the health risk assessment to human consumption. The concentration of heavy metals was analyzed by the flame atomic absorption spectrophotometer and the flow injection mercury system. The metal concentrations in prawns were detected in the following order: Hg < Cd < Co < Cu < Zn; in all body parts (P < 0.05), where a high concentration of metals was recorded in gills due to its function as the excretion site of contaminants from the body. A positive correlation of Co, Cu, and Zn in muscles and gills was observed with the increasing size of prawns. The human health risk assessment indicated a less potential adverse health effect of prawns for consumption from Sarawak River (hazard index values < 1). All samples contained metals below the Joint FAO/WHO Expert Committee on Food Additives (JECFA) permissible limit for human consumption (Cd < 2 mg/kg; Cu < 30 mg/kg; Zn < 150 mg/kg; Hg < 1 mg/kg). Therefore, M. rosenbergii from the Sarawak River were not contaminated enough to prevail high risk on human health, but regular monitoring is suggested for seasonal variability of heavy metals in M. rosenbergii for safeguarding human health.

Survey of mineral oil hydrocarbons in Chinese commercial complementary foods for infants and young children

ABSTRACT Recently, mineral oil hydrocarbons (MOH) in various foods have raised significant concern, especially for infants and young children due to their potential adverse health effects. Two fractions can be distinguished by certain analytical techniques, mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH). The toxicological profile of MOSH and MOAH differs greatly. The toxicity of MOSH is linked with long-term accumulation of some hydrocarbons. MOAH with three to seven, non- or simple-alkylated, aromatic rings may be mutagenic and carcinogenic. However, data on the occurrence of mineral oils in commercial complementary foods for infants and young children are lacking in China. In the present study, 100 commercial food samples were collected, including 26 pureed or paste canned foods, 21 high-protein ground cereal foods (rice flour), 25 raw cereal foods (noodles), and 28 cereal-based molar sticks and biscuits. The content of MOSH and MOAH in those samples was determined by optimised sample preparation methods combined with on-line high-performance liquid chromatography coupled with gas chromatography and flame ionisation detector (HPLC-GC-FID), with a limit of quantification of 0.5 mg/kg. The results indicated that there were no MOAH detected in any of the foods, but MOSH and polyolefin oligomeric saturated hydrocarbons (POSH) existed in most of the food samples, at <0.5–23.68 mg/kg. Moreover, the data and chromatograms of the MOSH and POSH also indicated that these contaminants were closely correlated to their ingredients and manufacturers. The current study provides basic data to understand MOH exposure and consequent health impact.

Chemical Characterization of Microcystis aeruginosa for Feed and Energy Uses

Cyanobacterial blooms and strains absorb carbon dioxide, drawing attention to its use as feed for animals and renewable energy sources. However, cyanobacteria can produce toxins and have a low heating value. Herein, we studied a cyanobacterial strain harvested during a bloom event and analyzed it to use as animal feed and a source of energy supply. The thermal properties and the contents of total nitrogen, protein, carbohydrate, fatty acids, lipid, and the presence of cyanotoxins were investigated in the Microcystis aeruginosa LTPNA 01 strain and in a bloom material. Microcystins (hepatotoxins) were not detected in this strain nor in the bloom material by liquid chromatography coupled to mass spectrometry. Thermogravimetric analysis showed that degradation reactions (devolatilization) initiated at around 180 °C, dropping from approximately 90% to 20% of the samples’ mass. Our work showed that despite presenting a low heating value, both biomass and non-toxic M. aeruginosa LTPNA 01 could be used as energy sources either by burning or producing biofuels. Both can be considered a protein and carbohydrate source similar to some microalgae species as well as biomass fuel. It could also be used as additive for animal feed; however, its safety and potential adverse health effects should be further investigated.

Evaluating adults' health-related values and preferences about unprocessed red meat and processed meat consumption: protocol for a cross-sectional mixed-methods study.

Background: People need to choose from a wide range of foods, and in addition to availability and accessibility, people's values and preferences largely determine their daily food choices. Given the potential adverse health consequences of red and processed meat and the limited knowledge on individuals' health-related values and preferences on the topic, such data would be useful in the development of recommendations regarding meat consumption. Methods and analysis: We will perform a cross-sectional mixed methods study. The study population will consist of adult omnivores currently consuming a minimum of three weekly servings of either unprocessed red meat or processed meat. We will explore participants' willingness to stop or reduce their unprocessed red meat, or their processed meat consumption through a direct-choice exercise. This exercise will consist of presenting a scenario tailored to each individual's average weekly consumption. That is, based on a systematic review and meta-analysis of the best estimate of the risk reduction in overall cancer incidence and cancer mortality, we will ask participants if they would stop their consumption, and/or reduce their average consumption. We will also present the corresponding certainty of the evidence for the potential risk reductions. Finally, we will measure their meat consumption three months after the interview and determine if they have made any changes to their average consumption. Ethics and dissemination: The research protocol was approved by the ethics committees in Canada (Research Ethics Board, Dalhousie University), Spain (Comitè Ètic d'Investigació Clínica de l'IDIAP Jordi Gol), Poland (The Bioethics Committee of the Jagiellonian University), and Brazil (National Research Ethics Commission). The study is based on voluntary participation and informed written consent. Results from this project will be disseminated through publications and presentations.

Evaluating adults’ health-related values and preferences about unprocessed red meat and processed meat consumption: protocol for a cross-sectional mixed-methods study

Background: People need to choose from a wide range of foods, and in addition to availability and accessibility, people’s values and preferences largely determine their daily food choices. Given the potential adverse health consequences of red and processed meat and the limited knowledge on individuals’ health-related values and preferences on the topic, such data would be useful in the development of recommendations regarding meat consumption. Methods and analysis: We will perform a cross-sectional mixed methods study. The study population will consist of adult omnivores currently consuming a minimum of three weekly servings of either unprocessed red meat or processed meat. We will explore participants’ willingness to stop or reduce their unprocessed red meat, or their processed meat consumption through a direct-choice exercise. This exercise will consist of presenting a scenario tailored to each individual’s average weekly consumption. That is, based on a systematic review and meta-analysis of the best estimate of the risk reduction in overall cancer incidence and cancer mortality, we will ask participants if they would stop their consumption, and/or reduce their average consumption. We will also present the corresponding certainty of the evidence for the potential risk reductions. Finally, we will measure their meat consumption three months after the interview and determine if they have made any changes to their average consumption. Ethics and dissemination: The research protocol was approved by the ethics committees in Canada (Research Ethics Board, Dalhousie University), Spain (Comitè Ètic d'Investigació Clínica de l'IDIAP Jordi Gol), Poland (The Bioethics Committee of the Jagiellonian University), and Brazil (National Research Ethics Commission). The study is based on voluntary participation and informed written consent. Results from this project will be disseminated through publications and presentations.

An Investigation and Optimization Study of Borax Content in the Slime Products

Abstract The slime toys were invented by Mattel in 1976. Slime is a reversible cross-linking gel made from guar gum. The slime toys were further developed into many different products and became one of the most popular toys. Despite buying slime products with attractive packaging, some children love to make their own slime products. The Consumer Council of Hong Kong published a report in 2019 to alert the public about the safety problems because of the excess boron content in those slime products in 2019. Some reported cases of slime product players having adverse health symptoms, which made the government concerned about the safety of slime products. The objectives of the study were to investigate the concentration of boron compounds in the four selected slime toys from the market and suggest a safety ingredient formula to the users to make their own do-it-yourself slime toys. Because only the European Union has relevant safety regulations and standards to limit the concentration of boron compounds in the toys, amendment of the current children’s products safety regulations and standards is required. By fulfilling the objectives, the consumers can make sure that their children who play with slime products can do so without the potential adverse health effects.