Anticoagulant Potency Research Articles

Ethnopharmacological relevanceIn traditional use, Panax medicinal plants (ginseng, red ginseng, notoginseng, Panax japonicus, and Panacis majoris rhizoma) have different bioactivities from each other, even under different dosages, but their chemical compositions are very similar; so the question is, what is the primary effective substance induced the different efficacy, and how to identify them from a group of chemical constituents? Aim of the studyThe goal of this research was to provide a strategy to determine the effective substance in Panax genus medicinal plants responsible for the anticoagulant response. Materials and methodsThis research used ultra-high performance liquid chromatography coupled with mass spectrometry (UPLC-MS) to analyze the saponin chemical compositions of different concentration ethanol extraction of notoginseng and the ginseng genus medical plant 75% ethanol extraction (Panax ginseng, red ginseng, Panax japonicus, Panacis majoris rhizome), and used four coagulation factors (PT, TT, APTT, Fib) to evaluate the anticoagulant activity of the extracts. Grey correlation analysis was applied to establish the spectral effect relationship and give the anticoagulant potency of different saponins. Network pharmacology and molecular docking were adopted to clarify and verify the possible mechanisms of anticoagulant action. ResultsThe results showed that the blood physiological regulation activities of Panax medicinal plants were different according to the solvent concentration, processing, species and dosage. Overall, the most suitable solvent for extraction of SQ was 75% ethanol; At low dosage (10–100 mg/mL), the anticoagulant effect of Panax medical plants was: ZJS > ZZS > SQ > RS > HS, and at high doses (100–1000 mg/mL) was: SQ > ZJS > ZZS > RS > HS. GRA and molecular docking results showed the contribution of some components (NG-R2, NG-Fc/G-Ra1/G-Ra2, G-Rc, G-Rk3, and G-Rh4) to the whole anticoagulant activity of the drug were increased, while the effect of CS-IVa was just decreased with the increase of dosage; the anticoagulant effect of G-Rg3 (the main anticoagulant component) is mainly related to the targets F2, AR, RHO, ACR, MB, GZMB, B2M, CA2, CAT, and PAPOLA. ConclusionThis study determined the effective substance of anti-coagulation of ginseng genus herbal medicines and the regulation of different anticoagulant effects of TCM by changing various influencing conditions, including processing method, extraction method, and dose. It also provided an effective strategy for effective substances identification of multicomponent, multifunction, and multipurpose herbal medicine.

Relevance. Hyper-coagulation processes increase the risk of coronary heart disease. Objective of the study was to study the effect of quercetin on the hemostatic system when using hyper-coagulation diet in rats. Materials and methods. The study included 17 male rats weighing 240-270 g. The rats of the control group (n = 6) were fed with conventional compound feed; Group I rats (n = 5) - mixed feed; and group II rats (n = 6) mixed with meat and quercetin (1.33 g/l). After 21 days, sterile blood was collected. The Amellung Coagulometer KS 4A studied the external and internal blood coagulation pathways, the activity of their own anticoagulant and fibrinolytic blood systems. The data obtained were statistically processed using Excel 2010 and Statistica v.10 (StatSoft, USA). Results. In rats of group 1, the external pathway of blood coagulation underwent the most significant changes, as indicated by an almost four-fold acceleration of prothrombin time and a decrease in the international normalized ratio. From the side of the internal pathway, the greatest changes were recorded in the last phase of clot formation: thrombin time decreased by 39.8% with an almost constant rate of activated partial thromboplastin time (APTT), which confirmed the activation of the fibrin formation phase. In addition, the content of soluble fibrin-monomer complexes (RFMC) increased 1.7 times and the fibrinogen decreased. At the same time, depletion of the anticoagulant potential of the blood was observed (a 42.2% decrease in the activity of the protein C system with a tendency to a decrease in antithrombin III (AT III). The fibrinolytic system was significantly suppressed, as indicated by more than three times inhibition of XII-dependent fibrinolysis. In animals Group 2 compared with the indicator in group 1, APTT was 2 times longer; the concentration of RFMC was also doubled, fibrinolysis was activated by 25%. Conclusion: The use of quercetin bioflavonoids in animals with simulated coronary heart disease slows down blood coagulation and potentiates the anticoagulant system, including due to a 40% increase in AT III activity, can be considered as a thrombosis-preventive effect

Anticoagulant Potency Research Articles

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Draft genome of the European medicinal leech Hirudo medicinalis (Annelida, Clitellata, Hirudiniformes) with emphasis on anticoagulants

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Anticoagulant Potency Research Articles

Related Topics

Articles published on Anticoagulant Potency

Production and Characterization of Melon Sulfated Pectin (Cucumis melo var. acidulus) Nanoparticles with Anticoagulant Potential

In Vitro Evaluation of Pro- and Anticoagulant Drugs in Children with End-Stage Liver Disease Undergoing Liver Transplantation.

Draft genome of the European medicinal leech Hirudo medicinalis (Annelida, Clitellata, Hirudiniformes) with emphasis on anticoagulants

Structural features, anti-coagulant and anti-adhesive potentials of blue crab (Portunus segnis) chitosan derivatives: Study of the effects of acetylation degree and molecular weight

Anticoagulant activity of black snake (Elapidae: Pseudechis) venoms: Mechanisms, potency, and antivenom efficacy

Anticoagulant active ingredients identification of total saponin extraction of different Panax medicinal plants based on grey relational analysis combined with UPLC-MS and molecular docking

Review of Therapeutic Options for the Prevention of VTE in Total Joint Arthroplasty.

Mechanisms Responsible for the Anticoagulant Properties of Neurotoxic Dendroaspis Venoms: A Viscoelastic Analysis.

Synthesis, bioactivities and cytogenotoxicity of animal fur-mediated silver nanoparticles

Green synthesized novel silver nanoparticles and their application as anticoagulant and thrombolytic agents: A perspective

Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigens.

Synthesis and Pharmacological Evaluation of Novel Coumarin Derivatives

Effects of Methanol Extract of Illicium verum Hook.f on Coagulation Parameters in Rabbits

Kemirici Zehirlerindeki Superwarfarine Maruziyet: Bir Ergende Uzun Süreli Kanama Kusuru Gelişmesi

Apixaban versus Warfarin in Patients with Left Ventricular Thrombus: A Pilot Prospective Randomized Outcome Blinded Study Investigating Size Reduction or Resolution of Left Ventricular Thrombus

Ultrasound-guided thrombin injection for treatment of iatrogenic femoral artery pseudoaneurysms compared with open surgery: first experiences from a single institution.

Effect of age on the functioning of hemostasis system during hip joint endoprosthesis

Polysaccharide composition of an anticoagulant fraction from the aqueous extract of Marsypianthes chamaedrys (Lamiaceae)

THROMBOSE-PREVENTIVE INFLUENCE OF QUERCETIN IN RATS ON THE HYPER-COAGULATION DIET

Influence of sulfated polysaccharides from Ulva lactuca L. upon Xa and IIa coagulation factors and on venous blood clot formation