Summary.1. A comparative study has been made during 1928–1933 of the aphis infestation at successful seed potato producing centres, where there has been no increase in virus infection among the stocks, and also at unsuccessful centres, where the increase in virus infection has been large and rapid.2. The technique developed for this study—one which is applicable for an aphis survey of prospective seed potato producing areas—is given. Identification, in the field, of the different species of aphides infesting potato is desirable in such a survey, and descriptions, with photographs, are presented.3. Ecological notes on the development of the aphis infestation show that M. persicae—the species that is generally responsible for the spread of virus diseases among potatoes—hibernates as apterae in considerable quantity on winter Brassicae, particularly savoys. These plants constitute the main source of alate spring migrants in most rural districts. The main migration of alatae takes place, normally, in early June, and, in North Wales, is largely from the east. Alatae are not common during July and August and do not increase until the late September migration from potatoes.4. The index figure of infestation at centres in which there was a rapid increase in virus diseases among the potato stocks always exceeded 100 M. persicae per 100 leaves, and attained such numbers as 1000 individuals per 100 leaves. At centres where there has been no increase in virus infection, the index figure of infestation has not exceeded 20 M. persicae per 100 leaves. The period at which this index figure should be obtained is important, and, with certain exceptions, mid‐July proved to be the most satisfactory. It is essential that the figure should be an index of the intensity of the initial infestation of alatae from outside Bources and their subsequent spread, and should not be simply an index of the rate of reproduction of aphides within the field.
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