Field trial was conduced on farming household in Jasenica (area of Bosnia and Herzegovina) with three potato varieties Solanum tuberosum L. (cv. Liseta, Cleopatra and Adora). The experiment was set up in randomized block design scheme in three replications. For side dressing we used three nitrogen doses (N 100, N 200 and N 300 kg N/ha) plus control plots (N 0 kg/ha). Data were being collected during two years 2004 and 2005. We applied half of dose of total nitrogen amount in pre-planting and the other half 45 days after the planting. The objective of this research was to estimate influence of different nitrogen doses on optimal tuber yield. We also established percentage of nitrogen, dry matter and raw ashes in potato tuber. We have observed numerical data on plant sites, number of haulm per plants and biomass of potato plant per treatments. Data were processed with SPSS software. Results of ANOVA test shows that nitrogen nutrition affects on the yield and other traits of potato crop. This research shows that timing and application of correct quantity of nitrogen nutrition results in yield increments.