Potato Clones Research Articles

Evaluation of Red Skinned Advance Potato (Solanum tuberosum L.) Clones for Tuber Yield and Yield Parameters in Eastern Indo-gangetic Plains of India

Potato is a very important tuber crop used as staple vegetable in almost every household. India ranks second in potato production after China. The production of potato increased significantly during last six decades. There is remarkable increase in potato yield and production during last decade 1.10 and 5.98% per annum respectively at national level (Rani & Prasoon, 2013). The important yield and yield attributing traits are plant emergence (%), number of tubers/plots, tuber yield/plot and tuber dry matter (%). The present experiment was conducted at experimental field of ICAR- Central Potato Research Institute Regional Station Patna, Bihar. In this experiment data were recorded for plant emergence (%), seed wt (t/ha), foliage senescence (%), total and marketable tuber yield (t/ha), dry matter content in percentage (tuber), late blight (%), leaf spot disease (%) during 75- and 90-days crop. The trial was conducted in randomized block design with three replications to evaluate nine advance clones namely MS/11-664, MS/13-527, MS/14-1381, MSP/16-307, MSP/16-375, PS/11-47, PS/13-45, CP-4409 and Memphis along with seven potato varieties Kufri Khyati, Kufri Lalima, Kufri Lalit, Kufri Manik, Kufri Mohan, Kufri Pukhraj and K Neelkanth as controls during 2020-21 & 2021-22. The advance clones PS/13-45 (36.97t/ha), PS/11-47 (35.87t/ha), MS/11-664 (35.39t/ha) were good candidates for future new potato varieties in Eastern Indo Gangetic plains of India.

Integrated transcriptomic and metabolomic analyses reveal transcriptional regulatory network for phenolic acid biosynthesis in potato tubers

The phenolic acid profile and concentration in potatoes signify their nutritional significance and functional value. In this study, a total of 398 metabolites, including 52 phenolic acids, were identified from three colored potato clones at three developmental stages using widely targeted metabolomics. Red and purple-fleshed potatoes exhibit higher levels of most phenolic acids compared to yellow-fleshed potatoes, particularly notable in their chlorogenic acid content. We concentrated on the distribution of differentially expressed transcription factors (TFs) among these clones, pinpointing the ERF (ethylene response factor), bHLH (basic helix-loop-helix), and MYB (myeloblastosis) as the predominant TFs via RNA-Seq analysis. Based on integrated transcriptomic and widely targeted metabolomic analysis, 23 pathway genes that might be involved in the biosynthesis of phenolic acid were identified. Furthermore, three central modules and several hub genes, encompassing 7 TFs, were identified. Notably, bHLH1 was identified as a key transcriptional regulator within this network, specifically activating the transcription of StCCoAOMT, a key enzyme catalyzing the methylation of caffeoyl-CoA to feruloyl-CoA. This study may provide new insights into the molecular mechanisms of phenolic acid biosynthesis in potatoes and potentially advances the development of functional food products derived from these potatoes.

Comprehensive genomic analysis and expression profiling of the cytochrome P450 genes during abiotic stress and flavonoid biosynthesis in potato (Solanum tuberosum)

The research focused on the cytochrome P450 (CYP) superfamily, a prominent enzyme family involved in plant metabolism. A total of 253 StCYP genes from the potato genome were investigated and characterized through various methods, including phylogentic analysis, physical and chemical characterization, chromosome localization, gene structure and conserved protein domain classification, gene duplication occurrences, collinearity and expression pattern analysis. The 253 StCYPs were classified into seven clans and 33 families, distributed unevenly across 12 chromosomes. Collinearity analysis revealed 28 pairs of orthologous genes between potato StCYPs and Arabidopsis AtCYPs. Moreover, the expression of the StCYPs in various tissue parts of doubled haploid (DM) potatoes under abiotic stress and exogenous hormone treatment was investigated by using RNA-seq data from the PGSC database. It was discovered that one StCYP gene likely contributes to drought stress response through the ABA-dependent pathway. Furthermore, RNA-seq analysis of pigmented tuber flesh from three potato clones at three developmental stages enabled the identification of StCYPs potentially implicated in the flavonoid biosynthesis in potato tubers. In particular, StCYP705A-like was further validated for its potential role in this process. Employing Gfanno software in conjunction with RNA-seq data, seven StCYP genes (Two StC4H, three StF3′H, one StFNS Ⅱ and one StF3′5′H) were identified as flavonoid biosynthetic pathway genes in potato. Additionally, five StCYPs in CYP 71 clan demonstrated a strong association with flavonoid biosynthesis. These findings lay a groundwork for a more comprehensive understanding of the StCYP gene family and further exploration of the functions of StCYP genes in potato abiotic stress tolerance and flavonoids biosynthesis.

QTL discovery for agronomic and quality traits in diploid potato clones using PotatoMASH amplicon sequencing.

We genotyped a population of 618 diploid potato clones derived from six independent potato-breeding programmes from NW-Europe. The diploids were phenotyped for 23 traits, using standardized protocols and common check varieties, enabling us to derive whole population estimators for most traits. We subsequently performed a genome-wide association study (GWAS) to identify quantitative trait loci (QTL) for all traits with SNPs and short-read haplotypes derived from read-backed phasing. In this study, we used a marker platform called PotatoMASH (Potato Multi-Allele Scanning Haplotags); a pooled multiplex amplicon sequencing based approach. Through this method, neighboring SNPs within an amplicon can be combined to generate multiallelic short-read haplotypes (haplotags) that capture recombination history between the constituent SNPs and reflect the allelic diversity of a given locus in a different way than single bi-allelic SNPs. We found a total of 37 unique QTL across both marker types. A core of 10 QTL was detected with SNPs as well as with haplotags. Haplotags allowed to detect an additional 14 QTL not found based on the SNP set. Conversely, the bi-allelic SNP set also found 13 QTL not detectable using the haplotag set. We conclude that both marker types should routinely be used in parallel to maximize the QTL detection power. We report 19 novel QTL for nine traits: Skin Smoothness, Sprout Dormancy, Total Tuber Number, Tuber Length, Yield, Chipping Color, After-cooking Blackening, Cooking Type, and Eye depth.

Yield performance and anthocyanin content of several purple-fleshed sweet potato clones grown in two locations in East Nusa Tenggara, Indonesia

Abstract. Arsa IGBA, Mau YS, Ndiwa ASS, Gandut YRY, Ishaq LF, Mahayasa INW. 2024. Yield performance and anthocyanin content of several purple-fleshed sweet potato clones grown in two locations in East Nusa Tenggara, Indonesia. Biodiversitas 25: 2276-2289. Several promising purple-fleshed sweet potato hybrid clones were identified to have high yield potential and, thus, can be evaluated for other superior traits and later be further processed for varietal registration and release. This study aimed to determine the tuber yield performance and anthocyanin content of several purple-fleshed sweet potato clones across two locations and to identify the best clones based on mean performance and stability. The study was conducted in the farmer's field in two different altitudes in West Timor, i.e., Mata Air Village, Kupang District (86 masl) and Netpala Village, Timor Tengah Selatan (TTS) District (1,090 masl) from April to September 2023. Six purple-fleshed sweet potato clones/varieties were assigned as treatment. The experiment was laid out in a complete block design in each location, and each treatment consisted of three replicates. Observed data included fresh storage root yield, number of storage roots per plant, storage root length, storage root tuber diameter, and tuber anthocyanin content. Observed data from the two trial locations were subjected to a combined ANOVA, cluster analysis, Principal Component Analysis (PCA), and GGE biplot analysis. The results showed that the genotype-by-location interaction effect was significant in all studied traits. Meanwhile, location or genotype significantly affected all traits except for the location, which did not significantly affect the anthocyanin content. The storage root yield ranged from ~19.95 t ha-1 to ~49.33 t ha-1, while the anthocyanin content ranged from ~28,64 mg/100 g to ~157,65 mg/100 g. The ranks of the tested genotypes based on the mean performance and stability are Undana UJ 7>Undana UJ 1>JPV-1>Antin 3>Undana UJ 6>Undana UJ 3 for storage root yield and JPV-1>Antin 3>Undana UJ 7>Undana UJ 6>Undana UJ 3>Undana UJ 1 for anthocyanin content.

Assessing heat tolerance in potatoes: Responses to stressful Texas field locations and controlled contrasting greenhouse conditions.

In recent years, heat stress has affected potato production more frequently, resulting in lower marketable yields and reduced tuber quality. In order to develop heat-tolerant potatoes, it is necessary to select under heat-stress conditions and consider traits affected by heat stress. The Texas A&M Potato Breeding Program has selected potatoes under high-temperature stress for several decades. Ten potato cultivars, representing heat tolerant and sensitive clones based on past performance in Texas, were included in field trials for three years at the two main locations used by the Texas Breeding Program (Dalhart and Springlake, TX) to assess if the Texas field locations are suitable for heat tolerance screening. Both locations were confirmed as appropriate for heat stress screening. However, Springlake was a more stressful location since it had significantly lower yields of marketable tubers and increased percentages of tuber defects. Planting time did not have a significant effect at the most stressful location. The same ten potato clones were included in greenhouse experiments with contrasting temperatures (normal versus heat stress). There was confirmation that heat stress conditions resulted in significantly lower marketable yields, specific gravity, dormancy, and significantly higher percentages of tuber defects; however, significant differences existed between potato clones. Under heat stress conditions, Russet Burbank had a high percent of tubers with external defects, whereas Atlantic showed the highest percentage of internal defects (mainly internal heat necrosis). Vanguard Russet produced the highest marketable yield while maintaining a low percentage of external and internal defects. Russet Burbank and Atlantic were heat-sensitive controls for external and internal tuber defects, respectively. In contrast, Vanguard Russet can be used as a reliable heat-tolerant control. Including appropriate controls in heat stress studies will help identify clones with heat tolerance.

Genome-wide identification and expression analysis of the SET domain-containing gene family in potato (Solanum tuberosum L.)

Genes containing the SET domain can catalyse histone lysine methylation, which in turn has the potential to cause changes to chromatin structure and regulation of the transcription of genes involved in diverse physiological and developmental processes. However, the functions of SET domain-containing (StSET) genes in potato still need to be studied. The objectives of our study can be summarized as in silico analysis to (i) identify StSET genes in the potato genome, (ii) systematically analyse gene structure, chromosomal distribution, gene duplication events, promoter sequences, and protein domains, (iii) perform phylogenetic analyses, (iv) compare the SET domain-containing genes of potato with other plant species with respect to protein domains and orthologous relationships, (v) analyse tissue-specific expression, and (vi) study the expression of StSET genes in response to drought and heat stresses. In this study, we identified 57 StSET genes in the potato genome, and the genes were physically mapped onto eleven chromosomes. The phylogenetic analysis grouped these StSET genes into six clades. We found that tandem duplication through sub-functionalisation has contributed only marginally to the expansion of the StSET gene family. The protein domain TDBD (PFAM ID: PF16135) was detected in StSET genes of potato while it was absent in all other previously studied species. This study described three pollen-specific StSET genes in the potato genome. Expression analysis of four StSET genes under heat and drought in three potato clones revealed that these genes might have non-overlapping roles under different abiotic stress conditions and durations. The present study provides a comprehensive analysis of StSET genes in potatoes, and it serves as a basis for further functional characterisation of StSET genes towards understanding their underpinning biological mechanisms in conferring stress tolerance.

A survey of the Sli gene in wild and cultivated potato.

Inbred-hybrid breeding of diploid potatoes necessitates breeding lines that are self-compatible. One way of incorporating self-compatibility into incompatible cultivated potato (Solanum tuberosum) germplasm is to introduce the S-locus inhibitor gene (Sli), which functions as a dominant inhibitor of gametophytic self-incompatibility. To learn more about Sli diversity and function in wild species relatives of cultivated potato, we obtained Sli gene sequences that extended from the 5'UTR to the 3'UTR from 133 individuals from 22 wild species relatives of potato and eight diverse cultivated potato clones. DNA sequence alignment and phylogenetic trees based on genomic and protein sequences show that there are two highly conserved groups of Sli sequences. DNA sequences in one group contain the 533 bp insertion upstream of the start codon identified previously in self-compatible potato. The second group lacks the insertion. Three diploid and four polyploid individuals of wild species collected from geographically disjointed localities contained Sli with the 533 bp insertion. For most of the wild species clones examined, however, Sli did not have the insertion. Phylogenetic analysis indicated that Sli sequences with the insertion, in wild species and in cultivated clones, trace back to a single origin. Some diploid wild potatoes that have Sli with the insertion were self-incompatible and some wild potatoes that lack the insertion were self-compatible. Although there is evidence of positive selection for some codon positions in Sli, there is no evidence of diversifying selection at the gene level. In silico analysis of Sli protein structure did not support the hypothesis that amino acid changes from wild-type (no insertion) to insertion-type account for changes in protein function. Our study demonstrated that genetic factors besides the Sli gene must be important for conditioning a switch in the mating system from self-incompatible to self-compatible in wild potatoes.

The Effects of Different GA3 and Mycorrhiza Dosages on Mini Tuber Production in Potatoes

This study was conducted in 2017 under greenhouse conditions using selected four different potato clones to determine the effects of different doses of GA3 and arbuscular mycorrhizal fungus on mini tuber production. The research, carried out in a randomized complete block design with three replications, applied GA3 doses of 0, 5, 10, and 15 ppm, and mycorrhizal inoculat doses of 0, 500, 1250, and 2000 mg/100 tubers. Parameters including emergence time, plant height, main stem number, tuber number, average tuber weight, tuber size distribution (>45 mm, 28-45 mm, <28 mm), and maturity period were examined. The effect of GA3 application on all investigated parameters except the number of main stems was significant, statistically. The highest mini tuber number (9.1 tubers) and mini tuber yield (408.4 g/pot) were obtained from the application of 15 ppm GA3, while the highest average mini tuber weight (46.74 g) was obtained from the control group. In mycorrhizal applications, the highest tuber number was obtained at a dose of 500 mg/100 tubers, and the highest mini tuber weight and yield were obtained at a dose of 1250 mg/100 tubers. As a result of the study, it was determined that the application of 15 ppm GA3 is suitable due to its positive effect on mini tuber multiplication, and the mycorrhizal application at a dose of 500 mg/100 tubers is appropriate due to its positive effect on increase of tuber number.

The effect of planting distance on the growth and yield of sweet potato clone Arf-03 on alluvial soil

Sweet potato (Ipomoea batatas L) is one of the alternative foods for food diversity. The need for sweet potatoes is increasing as consumption of materials and industrial raw materials have bright prospects, so various efforts are needed to increase production. One of the efforts to improve cultivation techniques to increase sweet potato production is by setting optimal planting spacing so that plant needs are met. Spacing is very closely related to nutrient absorption and photosynthesis processes, so plant growth increases. The purpose of this study was to determine the effect of planting distance on the growth and yield of ARF-03 clone sweet potatoes on alluvial soils and obtain optimal planting spacing for the growth and yield of sweet potatoes. The planting distance treatment given in this study was P1 = 20 cm × 100 cm, P2 = 30 cm × 100 cm, and P3 = 40 cm × 100 cm, using RAK as a reference for the placement of experimental units. The results of the growth of ARF-03 sweet potato plants on planting distance treatment showed that planting spacing had a significant effect on the length of the main tendrils, the number of branches, the fresh weight of the plant header, the dry weight of the plant header, the weight of tubers per plant, and the weight of tubers per bed. However, it has no real effect on the variables of the number of tubers, the length of the tubers, or the diameter of the tubers. The plant reaches its highest growth rate at a distance of 40 cm × 100 cm and its highest yield at a distance of 30 cm × 100 cm, with a bulb weight of 425.75 g per plant.

Changes in the content of carotenoids in potato tubers cultivated with or without the application of fungicides

AbstractIn this study, three cultivars and seven tetraploid potato clones were evaluated for two years in two cultivation systems (CS): without chemical protection against late blight development (NP) and with chemical protection (P). The objective of this study was to quantify the effect of chemical protection on the level of total carotenoids (TC) and individual carotenoids in potato tubers. The presented study showed that the content of TC in potato tubers is significantly affected by the CS and genotype. TC were higher in the system NP (mean 329.6 µg/100 g fresh weight [FW]) than in the system P (mean 206.2 µg/100 g FW). Although the development of late blight was observed only in the year 2019, a higher content of TC was observed in the system NP in both years. This dependence clearly show that CS without chemical protection favor carotenoids accumulation in potatotubers. In the case of lutein, the year had a significant impact on the concentration. The concentration of lutein in 2018 for the majority genotypes was higher (mean 72.1 µg/100 g FW), than in year 2019 (mean 37.3 µg/100 g FW), when late blight development wasobserved. The sharp decrease in lutein content in 2019 was probably caused by the development of late blight. A significant decrease of lutein content in potato tubers was observed in 2019 for all genotypes in system P and for five genotypes in system NP. It can be assumed that resistance mechanisms that occur during infection may have disturbed the accumulation of lutein in potato tubers.

Determining Adaptability of Potato Genotypes in Mount Elgon Region of Uganda

Potato (Solanum tuberosum L.) in Uganda is mainly produced in the highland areas of Kabale and Kisoro in south western and Bugisu and Sebei areas on the slopes of Mt. Elgon in the eastern part of the country. However, the yields have continuously reduced due to lack of suitable high yielding and disease resistant varieties. The purpose of this study was to identify high yielding disease resistant potato genotypes adapted to Mt. Elgon region. Eight CIP potato clones were evaluated alongside ten commonly grown Ugandan varieties in RCBD for two seasons at Buginyanya station, Bulambuli District. Results showed significant differences (P<0.05) in tuber size, tuber uniformity, marketable tuber yield and the total tuber yield among genotypes. Potato clones 392797.22 and 398208.29 produced significantly (P<0.001) higher tuber yield 44.8 t/ha and 39 t/ha respectively compared to the local check Cruza with 34.5 t/ha. rAUDPC for LB showed significant differences (P<0.001) among genotypes in both seasons. The most resistant genotypes were Kinigi and clone 399985.39 with rAUDPC of 0.0135 and 0.025 respectively whereas Bumbamagara (0.413) and 396036.201 (0.392) were the most susceptible. 396036.201(0.051) and Kinigi were the most resistant genotypes for bacterial wilt while Shangi (0.66) and Cruza (0.46) were the most susceptible to BW. Generally, genotypes 392797.22 and 398208.29 were the highest yielding and disease resistant hence recommended for release as commercial varieties.

Genetic Basis of Potato Tuber Defects and Identification of Heat-Tolerant Clones.

Heat stress during the potato growing season reduces tuber marketable yield and quality. Tuber quality deterioration includes external (heat sprouts, chained tubers, knobs) and internal (vascular discoloration, hollow heart, internal heat necrosis) tuber defects, as well as a reduction in their specific gravity and increases in reducing sugars that result in suboptimal (darker) processed products (french fries and chips). Successfully cultivating potatoes under heat-stress conditions requires planting heat-tolerant varieties that can produce high yields of marketable tubers, few external and internal tuber defects, high specific gravity, and low reducing sugars (in the case of processing potatoes). Heat tolerance is a complex trait, and understanding its genetic basis will aid in developing heat-tolerant potato varieties. A panel of 217 diverse potato clones was evaluated for yield and quality attributes in Dalhart (2019 and 2020) and Springlake (2020 and 2021), Texas, and genotyped with the Infinium 22 K V3 Potato Array. A genome-wide association study was performed to identify genomic regions associated with heat-tolerance traits using the GWASpoly package. Quantitative trait loci were identified on chromosomes 1, 3, 4, 6, 8, and 11 for external defects and on chromosomes 1, 2, 3, 10, and 11 for internal defects. Yield-related quantitative trait loci were detected on chromosomes 1, 6, and 10 pertaining to the average tuber weight and tuber number per plant. Genomic-estimated breeding values were calculated using the StageWise package. Clones with low genomic-estimated breeding values for tuber defects were identified as donors of good traits to improve heat tolerance. The identified genomic regions associated with heat-tolerance attributes and the genomic-estimated breeding values will be helpful to develop new potato cultivars with enhanced heat tolerance in potatoes.

New Potato Varieties Resistant to Late Blight and with High Quality for French Fries Generated in Peru

AbstractPotato is one of the main crops worldwide and generates income for small-scale farmers, and it is a primary component of the diet of rural inhabitants, especially in developing countries. In Peru, the demand for French fries in traditional restaurants, rotisserie chicken (“Pollerias”), and fast-food restaurants is increasing every day. However, supply is covered by imported pre-fried potatoes. During the 2019–2020 and 2020–2021 growing seasons, ten potato clones belong to the International Potato Center (CIP) breeding program, and two Peruvian varieties used for frying as controls UNICA (CIP392797.22) and INIA 303-CANCHAN (CIP380389.1) were tested in 13 trials in farmers' fields placed in the north, center, and south of Peru using a randomized complete block design with three replications of 150 plants. Late blight severity and yield were evaluated under field conditions, and the contents of dry matter and reducing sugars were determined with laboratory tests. Clones CIP395123.6, CIP396026.101, and CIP396034.103 were selected for their high level of resistance to late blight, yields over 30 t/ha, with more than 20% dry matter, less than 0.20% reducing sugars and excellent quality for French fries’ sticks. Selected clones have been registered in the official register of cultivars of Peru, as new varieties. The standard of living of small and medium-scale potato farmers can be improved with the production of these new varieties, which support the health of producers and consumers and the environment. New potato varieties can be used as parents in breeding programs around the world.

Genome-wide genetic architecture for plant maturity and drought tolerance in diploid potatoes.

Cultivated potato (Solanum tuberosum) is known to be highly susceptible to drought. With climate change and its frequent episodes of drought, potato growers will face increased challenges to achieving their yield goals. Currently, a high proportion of untapped potato germplasm remains within the diploid potato relatives, and the genetic architecture of the drought tolerance and maturity traits of diploid potatoes is still unknown. As such, a panel of 384 ethyl methanesulfonate-mutagenized diploid potato clones were evaluated for drought tolerance and plant maturity under field conditions. Genome-wide association studies (GWAS) were conducted to dissect the genetic architecture of the traits. The results obtained from the genetic structure analysis of the panel showed five main groups and seven subgroups. Using the Genome Association and Prediction Integrated Tool-mixed linear model GWAS statistical model, 34 and 17 significant quantitative trait nucleotides (QTNs) were found associated with maturity and drought traits, respectively. Chromosome 5 carried most of the QTNs, some of which were also detected by using the restricted two-stage multi-locus multi-allele-GWAS haploblock-based model, and two QTNs were found to be pleiotropic for both maturity and drought traits. Using the non-parametric U-test, one and three QTNs, with 5.13%-7.4% phenotypic variations explained, showed favorable allelic effects that increase the maturity and drought trait values. The quantitaive trait loci (QTLs)/QTNs associated with maturity and drought trait were found co-located in narrow (0.5-1kb) genomic regions with 56 candidate genes playing roles in plant development and senescence and in abiotic stress responses. A total of 127 potato clones were found to be late maturing and tolerant to drought, while nine were early to moderate-late maturing and tolerant to drought. Taken together, the data show that the studied germplasm panel and the identified candidate genes are prime genetic resources for breeders and biologists in conventional breeding and targeted gene editing as climate adaptation tools.

In vitro bioaccessibility of biguanide‐related compounds and phenolics from yellow, red, and purple‐fleshed potatoes

AbstractBackgroundDiabetes type II is growing rapidly as a result of the global shift in dietary and lifestyle habits. Although there are many pharmacological treatments for diabetes, incorporating foods with antidiabetic components into daily diet will be helpful. The present study compared the difference in the bioaccessibility of biguanide‐related compounds (BRCs, antidiabetic compounds) and phenolics from yellow, red, and purple potatoes.ResultsColor‐based averaged results showed that only biofortified red‐fleshed advanced potato clones showed higher bioaccessibility (14%) of BRCs compared to light yellow potatoes. Among all the tested compounds, only BRCs were released to maximum extent in the intestinal phase from all varieties and advance clones. This is the first report that revealed potatoes to be a good source of BRCs due to their high bioaccessibility. Bioaccessibility of phenolic compounds from boiled potatoes was 1.1‐fold higher in red colored, whereas almost 2‐fold higher in purple potatoes. Compared to light yellow potatoes, bioaccesibility of 3‐caffeoylquinic acid, 5‐O‐caffeoylquinic acid and 4‐O‐caffeoylquinic acid was 5.1, 5.7, 4.6 fold higher in boiled red potatoes, respectively, and 4.6, 3.7, and 6.9 fold higher in boiled purple potatoes, respectively. Total anthocyanins showed color‐dependent bioaccessibility and were 3.2 and 16.2 fold more bioaccessible from red and purple boiled potatoes, respectively, compared to light yellow potatoes.ConclusionDue to high bioaccessibility of phenolics, anthocyanins, and BRCs, purple and red potatoes can play pivotal role in health improvement of consumers worldwide. However, further detailed bioavailability studies are required to confirm these results.

Sensibilidade in vitro de plantas de batata expostas ao cádmio

ABSTRACT: Cadmium (Cd) is a heavy metal that is extremely dangerous to human health and can be found naturally in soils, deposited through industrial waste or phosphate fertilization. In this study, we evaluated the morphological responses of in vitro grown plants of potato in the presence of Cd, and define a procedure for assessing Cd sensitivity of different clones. The potato clone SMIJ461-1 was cultured in the presence of Cd at concentrations of 0, 20, 40, 60, 80, 90, 110, 120, 140, and 160 µM. Survival, rooting, number and height of shoots, leaf and root counts were evaluated at 7, 14, 21, and 28 days of cultivation. Adverse effects of Cd on the growth of the potato plants, exerted in a concentration-dependent manner, were observed for shoot height, leaf and root counts. Results of this study indicated that Cd concentrations of 0, 45, 90, and 135 µM can be used for future in vitro evaluation of potato clone sensitivity to Cd exposure.

Anthocyanin Profiles in Colored Potato Tubers at Different Altitudes by HPLC-MS Analysis with Optimized Ultrasound-Assisted Extraction.

The elevated anthocyanin content of colored potatoes produces numerous health benefits in humans. However, there is a paucity of studies exploring the influence of environmental factors on anthocyanin components in colored potatoes. In our work, the Box-Behnken design was adopted to optimize anthocyanin extraction from colored potato tubers with ultrasound assistance. The response surface model was stable and reliable (R2 = 0.9775), and under optimal extraction conditions, namely an ultrasonic power of 299 W, an extraction time of 10 min, and a solid-liquid ratio of 1:30 (g/mL), the yield reached 4.33 mg/g. Furthermore, the anthocyanins of colored potato tubers grown at different altitudes were determined by high-performance liquid chromatography-mass spectrometry with optimized ultrasound-assisted extraction, the results showed that anthocyanin levels were the highest at high altitudes, whereas anthocyanins were almost undetectable at mid-altitude. Moreover, the types of anthocyanin compounds present in colored potatoes varied at different altitudes. The red clones exhibited substantial accumulation of pelargonidin across all three altitudes. In contrast, the main anthocyanins found in purple clones were malvidin, petunidin, and cyanidin. We identified the anthocyanin components with a strong correlation to the environment, thereby establishing a fundamental basis for the breeding of potato clones with high anthocyanin content.

Application of potassium fertilizer and plant growth regulators to the growth and productivity of purple sweet potato

The study aimed to determine the effect of plant growth regulators and potassium fertilizer on the growth and productivity of three clones of purple sweet potato. The experiment was conducted at IPB University experimental station in Leuwikopo Dramaga Bogor for seven months. The study used a split-plot design with three factors and three replications. The first factor was as a subplot, namely plant growth regulators (PGR) application which consisted of two levels of without PGR (S0) and with PGR (S1) concentration of 2 cc l-1 of water (2,000 ppm). The second factor was as a subplot, namely rates of potassium chloride fertilizer (60, 120, 180 kg ha-1 K2O). The third factor as the main plot was sweet potato clones of (K1) Ayamurasaki, (K2) RIS-03063-05, and (K3) MSU 03028-10. The concentration of 2,000 ppm PGR did not significantly affect growth and tuber yield components. Potassium chloride K2O of 60 kg ha-1 up to 180 kg ha-1 did not significantly affect growth and tuber yield components. MSU 03028-10 clone had the highest total tuber yield (1537.8), healthy tuber (1529.9), unmarketable tuber (740.3), and small tuber (709.0). MSU 03028-10 clones have the longest stem length and the largest number of leaves 3-12 WAP. This study indicates that the MSU 03028-10 clone produces better growth and productivity than other clones.

Superior resistance to Phytophthora infestans in new pre-breeding potato clones under different nitrogen fertilisation regimes used in organic agriculture

ABSTRACT Late blight caused by Phytophthora infestans is the most devastating disease for cultivated potatoes, causing considerable financial loss annually due to loss of yield and costs of fungicide applications. For organic farming especially, the strict limitations on the use of fungicides makes research necessary to investigate factors that influence disease severity. Therefore, effects of nitrogen fertiliser rates on leaf and tuber blight in organically grown potato cultivars and pre-breeding clones were evaluated in the present study. It was found that the foliage of the pre-breeding clones was little, or not at all, affected by P. infestans, irrespectively of the nitrogen application rate, while the cultivars reacted differently with mild to strong late blight symptoms. Only some of the clones and cultivars showed increased infection of tubers at the higher nitrogen application rates, whilst most showed no significant differences between the N rates. Overall, the present study showed that the use of new resistant cultivars could be a suitable approach to reduce late blight in organic farming, where soil nitrogen levels may vary due to the use of different forms of nutrient inputs with varying mineralisation rates.