Background: E-learning has been introduced as a tool in thE-learning process in the majority of the international universities worldwide. Present study was done to develop and evaluate E-learning module to supplement traditional education in Pathology. Methods: A standardized E-learning module in Pathology was developed and validated on one ‘must know’ topic (Peripheral blood smear examination). All 2nd M.B.B.S. students (5th Semester) were subjected to a pre-test questionnaire, the E-learning module and a post-test questionnaire followed by feedback after obtaining their informed written consent. Feedback and suggestions given by the students were analysed. Results: Out of 145 participants, 98 were male and 47 were female. There was no statistically significant difference between perceptions of male and female participants. Comparison of pre and post test scores of students was done using Wilcoxon’s sign rank test. Students scored significantly more in post-test. Only 3% scored less in post-test and 5% had similar pre- and post-test scores. Frequency and median score of various parameters of feedback given by students showed positive perceptions. Conclusion: The students’ feedback showed that the E-learning method was a very effective way of teaching. In future E-learning should be implemented more frequently in Medical Education, which will create new and exciting opportunities for both educational institutions and students.