Postprandial Serum Triglyceride Research Articles

The effect of upper- and lower-body exercise on next-day postprandial triglycerides in healthy young men.

High non-fasting triglycerides (TG) concentration is linked to the development of atherosclerosis, and physical activity is commonly recommended to reduce postprandial TG concentration and cardiovascular diseases. Previous studies have demonstrated that acute whole-body (walking and running) or lower-body (leg cycling) aerobic exercise reduces postprandial TG. However, it is unclear whether upper-body exercise (i.e. arm-cranking) with sufficient energy expenditure lowers postprandial TG. Therefore, this study aimed to evaluate the effects of energy-matched upper- and lower-body exercises on postprandial TG concentrations the next day in healthy young men. Fifteen healthy young men (age 22.5 ± 1.7years, height 173.8 ± 5.7cm, body mass 68.2 ± 8.5kg, peak oxygen uptake 48.0 ± 5.5mL/min/kg and physically active) participated in a three-arm crossover trials: 1) arm-cranking, 2) leg-cycling exercise at 70% of mode-specific peak oxygen uptake to induce a net energy expenditure of 1,255kJ, or 3) rested between 16:00 and 17:00h on day 1 and consumed two standardised meals for breakfast (10:00h) and lunch (13:00h) on day 2. The mean macronutrient content of the breakfast was 44.9 ± 5.6g fat, 104.8 ± 13.0g carbohydrate, and 29.4 ± 3.6g protein, which provided 3.95 ± 0.49MJ energy (43% fat, 45% carbohydrate, and 12% protein), and that of the lunch was 45.2 ± 5.6g fat, 106.7 ± 13.2g carbohydrate, and 33.9 ± 4.2g protein, which provided 4.06 ± 0.50MJ energy (42% fat, 44% carbohydrate, and 14% protein). Time-averaged postprandial serum TG concentrations over 8h differed among trials (main effect of trial p < 0.001) and were lower in the upper- and lower-body exercise trials than in the control trial (1.46 ± 0.54 vs. 1.50 ± 0.69 vs. 1.79 ± 0.83mmol/L, respectively). The incremental TG area under the curve (AUC) (main effect of trial, p = 0.012) was 39% and 37% higher in the control trial than in the upper- and lower-body exercise trials (p = 0.025 and p = 0.033, respectively). There were no significant differences in incremental TG AUC between the upper- and lower-body exercise trials. An acute bout of energy-matched upper- and lower-body exercises similarly lowered postprandial TG concentrations the following day in healthy young men.Trial registration number: UMIN000045449.Date of registration: 10 September 2021.

Fat composition of milk replacer influences postprandial and oxidative metabolisms in dairy calves fed twice daily

Milk replacers (MR) for calves contain alternative fat sources as substitute for milk fat. This substitution leads to differences in fat properties, such as the fatty acid profile and the triglyceride structure. This study evaluated how fat composition in MR affects gastrointestinal health, blood redox parameters, and postprandial metabolism in calves fed twice daily. Forty-five individually housed male Holstein-Friesian calves (2.3 ± 0.85 d of age) were assigned to 1 of 15 blocks based on the age and the day of arrival. Within each block, calves were randomly assigned to 1 of 3 experimental diets and received their respective diet from arrival until 35 d after arrival. The 3 experimental diets (n = 15 per treatment group) consisted of an MR with a blend of vegetable fats containing rapeseed and coconut (VG), an MR with only animal fats from lard and dairy cream (AN), and an MR containing a mixture of animal and vegetable fats including lard and coconut (MX). The fatty acid profile of each MR was formulated to resemble that of bovine milk fat while using only 2 fat sources. All MR were isoenergetic, with 30% fat (% DM), 24% crude protein, and 36% lactose. Chopped straw and water were available ad libitum from arrival onward but no starter feed was provided. Daily milk allowances were 6.0 L from d 1 to 5, 7.0 L from d 6 to 9, and 8.0 L from d 10 to 35, divided into 2 equal meals and prepared at 135 g/L (13.5% solids). Fecal appearance was scored daily; calves were weighed and blood was drawn on arrival and weekly thereafter. Urine and feces were collected over a 24-h period at wk 3 and 5 to determine apparent total-tract digestibility and assess gastrointestinal permeability using indigestible markers. Postprandial metabolism was evaluated at wk 4 by sequential blood sampling over 7.5 h, and the abomasal emptying rate was determined by acetaminophen appearance in blood. Fat composition in MR did not affect growth, MR intake, gastrointestinal permeability, nor nutrient digestibility. The percentage of calves with abnormal fecal scores was lower at wk 2 after arrival in calves fed VG than MX, whereas AN did not differ from the other treatments. Calves fed AN and MX had higher thiobarbituric acid reactive substances measured in serum than VG, whereas plasma ferric-reducing ability was greater in calves fed MX than VG. Postprandial acetaminophen concentrations did not differ across treatment groups, but the area under the curve was smaller in calves fed VG than in the other 2 treatments, which is indicative of a slower abomasal emptying. Postprandial serum triglyceride concentration was greater in calves fed AN than VG, whereas MX did not differ from the other treatments. Based on these outcomes, all 3 fat blends can be considered suitable for inclusion in MR for calves.

Elevated postprandial triglyceride-rich lipoproteins in patients with diabetes and stable coronary artery disease correlated with early renal damage and systemic inflammation

BackgroundDyslipidaemia is key in the development of coronary heart disease (CHD) in patients with diabetes mellitus (DM). Accumulated evidence supports that diabetic nephropathy increases the mortality risk of patients with CHD, while the influence of diabetic dyslipidaemia on renal damage in patients with DM and CHD remains unknown. Moreover, recent data indicate that postprandial dyslipidaemia has predictive value in terms of CHD prognosis, especially in patients with DM. The study aimed to determine the relationship of triglyceride-rich lipoproteins (TRLs) after daily Chinese breakfast on systemic inflammation and early renal damage in Chinese patients with DM and SCAD.MethodsPatients with DM diagnosed with SCAD while in the Department of Cardiology of Shengjing Hospital from September 2016 to February 2017 were enrolled in this study. Fasting and 4-h postprandial blood lipids, fasting blood glucose, glycated haemoglobin, urinary albumin-to-creatinine ratio (UACR), serum interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) concentrations, and other parameters were measured. Fasting and postprandial blood lipid profiles and inflammatory cytokines were analysed using a paired t-test. The association between variables was analysed using Pearson or Spearman bivariate analysis. P < 0.05 was considered to be statistically significant.ResultsThe study enrolled 44 patients in total. Compared with fasting state, postprandial total cholesterol high-density lipoprotein-cholesterol (HDL-C),low-density lipoprotein-cholesterol (LDL-C) and non-high-density lipoprotein-cholesterol (non-HDL-C) all showed no significant change. Postprandial serum triglyceride (TG) concentration increased significantly compared with that at fasting (1.40 ± 0.40 vs. 2.10 ± 0.94 mmol/L, P < 0.001), as did serum remnant lipoprotein-cholesterol (RLP-C) (0.54 ± 0.18 mmol/L vs. 0.64 ± 0.25 mmol/L). Pearson analysis revealed that serum TG and RLP-C positively correlated before and after breakfast. Moreover, during fasting, positive correlations were observed between TG and serum IL-6, TNF-α, and UACR. Positive correlations were observed between RLP-C and IL-6, UACR under fasting condition, while both TG and RLP-C were positively correlated with postprandial serum IL-6, TNF-α, and UACR concentrations. Finally, positive correlations were observed between UACR and IL-6 and TNF-α concentration under both fasting and postprandial conditions.ConclusionsAn increase in postprandial TRLs was observed in Chinese patients with DM and SCAD after daily breakfast, and this increase may be related to early renal injury via the induction of systemic inflammation.

Intestinal SEC16B modulates obesity by regulating chylomicron metabolism

ObjectiveGenome-wide association studies (GWAS) have identified genetic variants in SEC16 homolog B (SEC16B) locus to be associated with obesity and body mass index (BMI) in various populations. SEC16B encodes a scaffold protein located at endoplasmic reticulum (ER) exit sites that is implicated to participate in the trafficking of COPII vesicles in mammalian cells. However, the function of SEC16B in vivo, especially in lipid metabolism, has not been investigated. MethodsWe generated Sec16b intestinal knockout (IKO) mice and assessed the impact of its deficiency on high-fat diet (HFD) induced obesity and lipid absorption in both male and female mice. We examined lipid absorption in vivo by acute oil challenge and fasting/HFD refeeding. Biochemical analyses and imaging studies were performed to understand the underlying mechanisms. ResultsOur results showed that Sec16b intestinal knockout (IKO) mice, especially female mice, were protected from HFD-induced obesity. Loss of Sec16b in intestine dramatically reduced postprandial serum triglyceride output upon intragastric lipid load or during overnight fasting and HFD refeeding. Further studies showed that intestinal Sec16b deficiency impaired apoB lipidation and chylomicron secretion. ConclusionsOur studies demonstrated that intestinal SEC16B is required for dietary lipid absorption in mice. These results revealed that SEC16B plays important roles in chylomicron metabolism, which may shed light on the association between variants in SEC16B and obesity in human.

ANGPTL3 Is Involved in the Post-prandial Response in Triglyceride-Rich Lipoproteins and HDL Components in Patients With Coronary Artery Disease.

It is well-established that there exists an inverse relationship between high-density lipoprotein (HDL) cholesterol and triglyceride (TG) levels in the plasma. However, information is lacking on the impact of post-prandial triglyceride-rich lipoproteins (TRLs) on the structure of HDL subclasses in patients with coronary artery disease (CAD). In this study, the data of 49 patients with CAD were analyzed to evaluate dynamic alterations in post-prandial lipid profiles using nuclear magnetic resonance-based methods. An enzyme-linked immunosorbent assay was used to quantify the serum angiopoietin-like protein 3 (ANGPTL3). After glucose supplementation, the expression of hepatic ANGPTL3 was evaluated both in vitro and in vivo. Compared to fasting levels, the post-prandial serum TG level of all participants was considerably increased. Although post-prandial total cholesterol in HDL (HDL-C) remained unchanged, free cholesterol in HDL particles (HDL-FC) was significantly reduced after a meal. Furthermore, the post-prandial decrease in the HDL-FC level corresponded to the increase in remnant cholesterol (RC), indicating the possible exchange of free cholesterol between HDL and TRLs after a meal. Moreover, CAD patients with exaggerated TG response to diet, defined as TG increase >30%, tend to have a greater post-prandial increase of RC and decrease of HDL-FC compared to those with TG increase ≤30%. Mechanistically, the fasting and post-prandial serum ANGPTL3 levels were significantly lower in those with TG increase ≤30% than those with TG increase >30%, suggesting that ANGPTL3, the key lipolysis regulator, may be responsible for the different post-prandial responses of TG, RC, and HDL-FC.

Riceberry rice beverage decreases postprandial glycemic response, inflammatory markers and antioxidant status induced by a high-carbohydrate and moderate-fat meal in overweight and obese men.

Recent clinical studies support the beneficial role of riceberry rice and its food products in controlling the glycemic response in healthy subjects. The aim of the current work was to determine the effects of riceberry rice beverage (RRB) on postprandial glycemic, insulin and triglyceride responses, inflammatory biomarkers, and antioxidant status as well as appetite sensation following a high-carbohydrate and moderate-fat (HCMF) meal in overweight/obese subjects. Thirteen participants, overweight and obese men (age = 24.46 ± 0.90 years and BMI = 25.92 ± 0.69 kg m-2), completed a randomized, crossover intervention study. They consumed an HCMF meal with or without RRB (2 g of riceberry rice extract powder/400 ml). Blood samples and appetite sensation were measured at the fasting state and up to 6 h after meal consumption. The ingestion of the HCMF meal accompanied by RRB had a lower incremental area under the curve (iAUC) for postprandial plasma glucose, insulin, malondialdehyde (MDA) and serum triglyceride concentrations when compared to the control. A significant increase in the ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), and thiol level, all measures of antioxidant capacity, was also observed in the participants who consumed the HCMF meal accompanied by RRB. The postprandial level of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) significantly increased at 3 h and 6 h after the HCMF meal intake when compared to the fasting state. Interestingly, these values were significantly decreased upon consumption of RRB. However, there were no statistically significant differences in the rating scores of hunger, fullness, desire to eat, and satiety among the tested meals. In conclusion, RRB intake prevented HCMF meal-induced postprandial glycemic, lipemic, and pro-inflammatory responses and improved plasma antioxidant capacity in overweight and obese participants.

Study of Biochemical Parameters in Diabetic Patients with and without Diabetic Retinopathy – A Hospital based study

Background: Diabetic Retinopathy (DR) is a common, potentially blinding and visually disabling complication of diabetes. Early detection of retinopathy and its progression to severity with routine referral for screening by Ophthalmologist can save vision by timely management. Methods: This was a hospital based cross-sectional study done to study the association of glycated hemoglobin (HbA1c), fasting and postprandial blood sugar, serum lipid profile, serum creatinine and urine albumin in diabetic patient with and without DR. A total of 50 patients with Diabetic retinopathy and 50 patients without diabetic retinopathy were enrolled in this study. All patients included were Type 2 DM aged 35 years and above. Results: The mean HbA1c was 8.62±1.5 and 5.54±1.2, total cholesterol was 228.9±63 and 184.9±39.8 mg/dl, serum triglyceride was 226.6±80.7 and 160.8±45.1 mg/dl, LDL-C was 152.3±49 and 127.2±37 mg/dl and serum creatinine was 1.15±0.45 and 0.66±0.27 mg/dl in diabetic retinopathy group and no diabetic retinopathy group respectively. There was significant association of elevated HbA1c, serum triglyceride, LDL- C and total cholesterol with diabetic retinopathy in patients with type 2 DM. The mean values of serum lipoproteins were higher in the diabetic retinopathy group. Conclusion: Elevated fasting and postprandial blood sugar, glycated hemoglobin, total cholesterol, serum triglyceride, LDL-C, serum creatinine and urine albumin were significantly associated with DR in our study. So, all patients with diabetes mellitus should be screened routinely for serum lipoproteins along with blood sugar profile, as it would help in early detection of diabetic retinopathy.

Effects of interrupting sitting with different activity bouts on postprandial lipemia: A randomized crossover trial.

To determine whether interrupting prolonged sitting with three different activity breaks has both acute and chronic effects on postprandial lipid metabolism immediately after the activity-break period and on the following day, this study is a secondary analysis of an experimental research, which included 16 sedentary healthy adults (7 men, 24±3years, BMI 22.2±2.3kg/m2 ) who completed four 26-h laboratory trials. Participants spent 22.5hours in a whole room calorimeter for testing energy expenditure (EE), including a 9-h activity-break period: (a) 9-h prolonged sitting (SIT); (b) 3minutes of brisk walking (60% VO2max ) in between every 30-min sitting bout (WALK3), (c) 5minutes every 45-min (WALK5), and (d) 8minutes every 60-min (WALK8). Total area under the curve (tAUC) and incremental AUC (iAUC) for 2-h postprandial serum triglyceride (TG) levels and non-esterified fatty acid (NEFA) levels were examined immediately after the 9-h trial (post-dinner) and the next morning (post-breakfast). WALK8 reduced the post-breakfast TG tAUC by 11% (P=.041) relative to SIT, and the effect was attenuated after adjustment for EE. The tAUC and iAUC indicated no significant treatment effects on post-dinner TG and NEFA, and post-breakfast NEFA in any of the activity-break trials. EE was positively associated with the post-breakfast NEFA iAUC (unstandardized β=0.17µmol/L/MJ [0.05-0.28], P=.006). There was a chronic effect of interrupting sitting with short bouts (8minutes) of brisk walking every 60minutes on postprandial lipemia the following morning after intervention, and higher activity bout-induced EE may be more effective in sedentary, healthy adults.

Thresholds for postprandial hyperglycemia and hypertriglyceridemia associated with increased mortality risk in type2 diabetes patients: A real-world longitudinal study.

Aims/IntroductionTo identify thresholds for postprandial hyperglycemia and hypertriglyceridemia predictive of all‐cause mortality in patients with type 2 diabetes.Materials and MethodsA total of 1,928 patients with type 2 diabetes visited our clinic for the first time from 1995 to 1999 and were followed up for ≥1 year. During the first year, 2‐h post‐breakfast blood glucose (2h‐BG) levels were measured in 1,122 patients (BG cohort) and postprandial serum triglyceride (ppTG) levels were measured in 1,826 patients (TG cohort). Patients were retrospectively followed until 2017 and administered questionnaires. Associations between 2h‐BG and ppTG levels and mortality risk were assessed by the multivariate Cox regression analysis.ResultsOver of 17,429 person‐years, 162 deaths occurred in the BG cohort, and over 28,026 person‐years, 253 deaths occurred in the TG cohort. Hazard ratios (HRs) with 95% confidence intervals for all‐cause mortality per 1‐standard deviation increases in 2h‐BG and ppTG were 1.34 (1.08–1.67) and 1.24 (1.06–1.45), respectively. HRs showed increasing trends across quintiles of 2h‐BG (P = 0.034) and ppTG (P = 0.007). The HR was significantly elevated (2.37, 1.26–4.47) in the fifth quintile of 2h‐BG (≥13.8 mmol/L) compared with the first quintile (<7.0 mmol/L; P = 0.008). The HR was also significantly elevated (1.63, 1.03–2.60) in the fifth quintile of ppTG (≥2.30 mmol/L) compared with the first quintile (<0.91 mmol/L; P = 0.038).ConclusionsPostprandial hyperglycemia and hypertriglyceridemia were associated with all‐cause mortality in patients with type 2 diabetes. We propose thresholds of 13.8 mmol/L 2h‐BG and 2.30 mmol/L ppTG to identify patients at increased risk of mortality.

CTRP12 inhibits triglyceride synthesis and export in hepatocytes by suppressing HNF-4α and DGAT2 expression.

C1q/TNF-related protein 12 (CTRP12) is an antidiabetic adipokine whose circulating levels are reduced in obesity and diabetes. Although partial and complete loss-of-function mouse models suggest a role for CTRP12 in modulating lipid metabolism and adiposity, its effect on cellular lipid metabolism remains poorly defined. Here, we demonstrate a direct action of CTRP12 in regulating lipid synthesis and secretion. In hepatoma cells and primary mouse hepatocytes, CTRP12 treatment inhibits triglyceride synthesis by suppressing glycerophosphate acyltransferase (GPAT) and diacylglycerol acyltransferase (DGAT) expression. CTRP12 treatment also downregulates the expression of hepatocyte nuclear factor-4α (HNF-4α) and its target gene microsomal triglyceride transfer protein (MTTP), leading to reduced very-low-density lipoprotein (VLDL)-triglyceride export from hepatocytes. Consistent with the invitro findings, overexpressing CTRP12 lowers fasting and postprandial serum triglyceride levels in mice. These results underscore the important function of CTRP12 in lipid metabolism in hepatocytes.

Genome-wide Association Study of the Postprandial Triglyceride Response Yields Common Genetic Variation in Hepatic Lipase (LIPC)

The increase in serum triglyceride (TG) concentrations in response to a meal is considered a risk factor for cardiovascular disease. We aimed to elucidate the genetics of the postprandial TG response through genome-wide association studies (GWAS). Participants of the NEO (Netherlands Epidemiology of Obesity) study (n=5630) consumed a liquid mixed meal after an overnight fast. GWAS of fasting and postprandial serum TG at 150 minutes were performed. To identify genetic variation of postprandial TG independent of fasting TG, we calculated the TG response at 150 minutes by the residuals of a nonlinear regression that predicted TG at 150 minutes as a function of fasting TG. Association analyses were adjusted for age, sex, and principal components in a linear regression model. Next, using the identified variants as determinants, we performed linear regression analyses on the residuals of the postprandial response of 149 nuclear magnetic resonance-based metabolite measures. GWAS of fasting TG and postprandial serum TG at 150 minutes resulted in completely overlapping loci, replicating previous GWAS. From GWAS of the TG response, we identified rs7350789-A (allele frequency=0.36), mapping to hepatic lipase (LIPC), to be associated with a smaller increase in TG concentrations at 150 minutes (β=-0.11; P-value=5.1×10-8). Rs7350789-A was associated with responses of 33 metabolite measures (P-value <1.34×10-3), mainly smaller increases of the TG-component in almost all HDL (high-density lipoprotein) subparticles (HDL-TG), a smaller decrease of HDL diameter and smaller increases of most components of VLDL (very low density lipoprotein) subparticles. GWAS of the TG response identified a variant near LIPC as a main contributor to postprandial TG metabolism independent of fasting TG concentrations, resulting in smaller increases of HDL-TG and VLDL subparticles.

Energy replacement diminishes the postprandial triglyceride-lowering effect from accumulated walking in older women.

Dietary replacement of an acute exercise-induced energy deficit offsets the postprandial triglyceride (TG)-lowering effect of exercise in young boys and middle-aged men. It is unclear whether these findings are observed when exercise is accumulated in older adults. This study examined the effect of accumulating short bouts of exercise, with and without dietary replacement of an exercise-induced energy deficit, on postprandial TG in older women. Seventeen older women (≥ 65years) underwent three, 8-h trials: (1) control, (2) accumulated walking and (3) accumulated walking with energy replacement. During the control trial, participants rested for 8h. The accumulated walking trials comprised twenty 1.5min brisk walking bouts performed at a pre-determined self-selected pace separated by 15min seated rest. In each trial, participants consumed a standardised breakfast and lunch. The breakfast in the accumulated walking with energy replacement trial included replacement of the energy deficit (0.62MJ, 149kcal) induced by exercise. Venous blood samples were collected fasted and at 2, 4, 6 and 8h after breakfast. Time-averaged postprandial serum TG concentrations over 8h were lower after accumulated walking than control and accumulated walking with energy replacement (mean ± SD: 1.46 ± 0.93 vs 1.71 ± 1.01 vs 1.60 ± 0.98mmol/L, respectively: main effect of trial p = 0.017). There was little difference between control and accumulated walking with energy replacement. Replacing the energy expenditure induced by accumulating 30min of brisk walking in short (1.5min) bouts diminishes the postprandial TG-lowering effect in older women.

Reduced postprandial serum triglyceride after a meal prepared using hot air frying: A randomized crossover trial

BackgroundElevated circulating post–meal triglyceride concentrations or postprandial triglyceride (ppTG) is an independent contributor to risk for coronary heart disease. Reliable ppTG reduction can be achieved by lowering the fat content of ingested meals. ObjectiveWe hypothesized that a commonly consumed fried meal prepared by a hot air frying technique, which facilitates lower cooking oil usage, would result in lower ppTG responses than the same meal prepared by conventional deep fat frying. DesignWe conducted a randomized crossover mixed meal test trial in young healthy male (n = 10) and female subjects (n = 5) in which serial blood samples (baseline, 30, 60, 90, 120, 150, 180, 240, 300, 360, 420 mins) were collected for biochemical analysis before and after administration of the meals. ppTG response was calculated as the 420 min. Incremental area under the curve for serum TG concentrations after meal ingestion. Participants and analysts were blinded to the meal type under study. ResultsSubjects' habitual diets were similar before each of the meal tests. We found that hot air frying led to a 74% reduction in the total fat content of the meal which resulted in a 67% reduction in the ppTG response (0.9 mmol∙h/L, 95%CI 0.4–1.5) compared to deep fat frying (3.8 mmol∙h/L, 95%CI 2.0–5.6, P < .01). ConclusionsHot air frying of a common meal led to lower ppTG responses in healthy subjects. Our results demonstrate that the oil reductions concomitant with hot air frying of a common meal translate into significant reductions in ppTG.

Dietary fructose and dyslipidemia: new mechanisms involving apolipoprotein CIII.

Chronic consumption of fructose and fructose-containing sugars leads to dyslipidemia. Apolipoprotein (apo) CIII is strongly associated with elevated levels of triglycerides and cardiovascular disease risk. We reviewed the effects of fructose consumption on apoCIII levels and the role of apoCIII in fructose-induced dyslipidemia. Consumption of fructose increases circulating apoCIII levels compared with glucose. The more marked effects of fructose compared with glucose on apoCIII concentrations may involve the failure of fructose consumption to stimulate insulin secretion. The increase in apoCIII levels after fructose consumption correlates with increased postprandial serum triglyceride. Further, RNA interference of apoCIII prevents fructose-induced dyslipidemia in nonhuman primates. Increases in postprandial apoCIII after fructose, but not glucose consumption, are positively associated with elevated triglycerides in large triglyceride-rich lipoproteins and increased small dense LDL levels. ApoCIII might be causal in the lipid dysregulation observed after consumption of fructose and fructose-containing sugars. Decreased consumption of fructose and fructose-containing sugars could be an effective strategy for reducing circulating apoCIII and subsequently lowering triglyceride levels.

Comparison of Fasting and Non-fasting Lipid Profile in Young Healthy Adults

Background and Aim: It is necessary to find out whether fasting blood samples are must for lipid profile determination. Aim of this study to find out is there any difference between fasting and non-fasting lipid profile in young healthy adults. Methods: This study was done on 100 MBBS students of medical college. Lipid profile was done in these healthy young adults for fasting and postprandial statuses. Results: The lipid profile parameters were compared in both the groups of fasting and postprandial statuses. In fasting group, the mean total cholesterol level was 192.1 mg/dl and mean postprandial total cholesterol level was 194.98 mg/dl (P = 0.0407). The mean fasting serum triglyceride level was 121.16 mg/dl and mean postprandial serum triglyceride level was 126.18 mg/dl (P = 0.0001). The mean fasting high density lipoprotein (HDL) level was 45.08 mg/dl and mean postprandial HDL was 43.84 mg/dl (P = 0.0656). The mean fasting serum VLDL level was 24.23 mg/dl and mean postprandial VLDL level was 25.24 mg/dl (P = 0.0001). The mean fasting LDL was 122.8 mg/dl and mean postprandial LDL was 125.9 mg/dl (P = 0.0416). Conclusion: Finally, from this study we found that there is no significant clinical difference between fasting and non-fasting levels of total cholesterol, HDL, LDL, VLDL and TG. Thus, for estimation of lipid profile we can use the blood samples at any time or irrespective of mealtime.

Hematologic and Serological Investigation of Effect on Gyeongokgo in Healthy Individuals : a Randomized, Subject-assessor-blind, Placebo-controlled, Single-center Pilot Study

There are no published data on Gyeongokgo (GOK) safety or efficacy despite being commonly use. The Gyeongokgo (GOK) is commonly used in traditional Korean medicine to promote a health qi and blood, but their objective data was not sufficient in clinical field. To investigate the safety and efficacy of GOK with hematologic and serologic testing and the change of the quality of life in healthy individuals. Randomized, subject-assessor-blind, placebo-controlled, single-center pilot study Participants and Interventions 29 healthy volunteer subjects were randomly placed into the GOK group (n = 20) or placebo control group (n = 9) and instructed to take one treatment packet (GOK or placebo) twice daily for 4 weeks. Subjects were assessed using the Fatigue Severity Scale (FSS) and Short Form 36 Health Survey (SF-36) and underwent hematologic and serologic tests and body composition analysis. The FSS total score (p = 0.093) and SF-36 general health index (p = 0.002) were improved following treatment in the GOK group. Post-treatment thyroid-stimulating hormone levels were increased in the GOK group compared with pre-treatment levels (p = 0.0042). C-reactive protein levels decreased (p = 0.0256) in the GOK group compared with that the placebo group. In time-series tests, GOK did not affect post-prandial serum triglycerides, glucose, insulin, or C-peptide levels. Notably, elevations in serum fasting triglycerides at 2- (p = 0.0333) and 4-hours (p = 0.0414) post-prandial were lower than those in the placebo group. GOK reduced fatigue levels and did not significantly affect laboratory test results performed to measure safety, serum glucose, and lipid profiles. Post-meal triglyceride levels were effectively reduced with treatment.

PT04.5: Reduced Postprandial Serum Triglyceride After a Meal Prepared Using Hot Air Frying: A Randomized Crossover Trial

PT04.5: Reduced Postprandial Serum Triglyceride After a Meal Prepared Using Hot Air Frying: A Randomized Crossover Trial

A study on correlation between fasting &amp;amp; post-prandial serum triglycerides and severity of stroke-at a tertiary care hospital

Aim & Objective of the study: The aim of this study is to investigate if serum TG levels predict stroke severity on admission with correlation of fasting and post-prandial serum TG levels Results: Of the 80 patients studied, 54 were males and 26 were females. Among the males, 42 had FTG≤2.3mmol/l and 12 had FTG levels >2.3mmol/l & 27 had PPTG≤2.3mmol/l and 27 had PPTG levels >2.3mmol/l. Of the 26 females 18 had FTG≤2.3mmol/l and 8 patients had FTG levels >2.3mmol/l & 15 had PPTG≤2.3mmol/l and 11 had PPTG levels >2.3mmol/l. In this study the number of patients with severe stroke (SSS≤25) were 20 of which 15 had F TG≤2.3mmol/l and only 5 patient had F TG>2.3mmol/l & 9 had PPTG≤2.3mmol/l and 12 had PPTG>2.3mmol/l. Out of 80 patients, 44 were hypertensive and 32 had FTG≤2.3mmol/l and 12 had FTG>2.3mmol/l & 19 had PPTG≤2.3mmol/l and 25 had PPTG>2.3mmol/l of the remaining 36 non hypertensive patients, 30 had FTG≤2.3mmol/l and 6 had FTG>2.3mmol/l & 25 had PPTG≤2.3mmol/l and 11 had PPTG>2.3mmol/l. 16 were IHD patients of which 12 had FTG≤2.3mmol/l and 4 had FTG>2.3mmol/l & 8 had PPTG≤2.3mmol/l and 8 had PPTG>2.3mmol/l & of the remaining 64 patients, 50 had FTG≤2.3mmol/l and 14 had FTG>2.3mmol/l & 30 had PPTG≤2.3mmol/l and 34 had PPTG>2.3mmol/l. The levels of serum FTG were not influenced by hypertension, IHD, age and sex of an individual but the level of serum PPTG were influenced by HTN. Conclusion: 1 In this study, mean serum fasting & post prandial serum triglyceride levels were not significantly associated with the severity of the stroke. As per this study hypertension, IHD, age and sex of an individual do not influence levels of serum fasting triglyceride levels but the serum post prandial triglyceride level were influenced by HTN.

Effects of Three Regular Activity Breaks on Postprandial Triglyceride Response in Healthy Young Adults

PURPOSE: To determine whether interrupting prolonged sitting with three kinds of regular walking activity breaks has an immediate or delayed effect on postprandial triglyceride response. METHODS: In a randomized crossover trial, 16 inactive healthy adults (7 men, aged 21-30 years) completed four 26-h (from 8:00 AM on day 1 to 10:00 AM on day 2) laboratory conditions. Except for the 9-h intervention phase, the same procedure was used in the following four trials: (1) 9-h prolonged sitting (SIT); (2), (3), and (4) sitting with 3, 5, and 8 minutes of brisk walking (60% VO2max) every 35, 50, and 70 minutes, respectively (WALK3, WALK5, and WALK8). Postprandial serum triglyceride (TG) and nonesterified fatty acid (NEFA) were measured for 2-h dinner immediately on day 1 and for 2-h breakfast on day 2. Meals and meal times were standardized across the conditions for all the participants. RESULTS: Compared with SIT, only WALK8 significantly attenuated 2-h breakfast postprandial triglyceride total area under the curve (tAUC; SIT: median [Q1, Q2], 2.12 mmol·h·L−1 [1.46, 3.67] vs WALK8: 2.01 [1.25, 3.34], p = 0.041). The tAUC for 2-h dinner postprandial triglyceride and for both 2-h dinner and breakfast postprandial NEFA were not significantly changed in the three-activity break conditions. However, compared with SIT, the three-activity break conditions significantly increased the pre-dinner NEFA concentrations on day 1 (WALK3 52%, WALK5 36%, and WALK8 75%; all p < 0.05), but only WALK8 increased the fasting NEFA concentration on day 2 (25%; p < 0.05). No significant differences in all the above-mentioned indicators were found among the three-activity break conditions. CONCLUSIONS: The 8-min brief bouts every 70 min attenuated the postprandial triglyceride response measured about 24 h after, not immediately after, the intervention phase. Supported by the Shanghai Science and Technology Committee (No. 16080503300).

Reduced Postprandial Serum Triglyceride After a Meal Prepared Using an Airfryer: A Randomized Crossover Trial (P08-103-19)

ObjectivesElevated circulating non-fasting triglyceride (TG) or postprandial triglyceride (ppTG) is as an independent contributor to risk for coronary heart disease. We hypothesized that a commonly consumed fried meal (including French fries and chicken nuggets) prepared by airfrying would result in a significantly lower ppTG response than the same meal prepared using a conventional deep fat frying approach. MethodsWe conducted a randomized crossover trial to evaluate the effect of airfryer cooking compared to conventional deep fat frying preparation in young healthy male (n = 10) and female subjects (n = 6). A 7-day washout period was included between meal test days. All study participants were blinded to the method of meal preparation. ResultsThe airfryer resulted in a significantly lower postprandial triglyceride response compared to deep fat frying of the same meal (P < 0.001). Glucose, insulin, and free fatty acid response differences were not found. Self-reported satiety did not differ after the meals. Furthermore, air-frying did not influence circulating inflammatory biomarkers, cholesterol, or apolipoproteins. ConclusionsAir frying of common fried meals results in lower postprandial triglyceride excursions in young healthy volunteers in the absence of any effects on satiety. Air-frying represents a useful and practical method to reduce meal fat content in line with current dietary recommendations on triglyceride management and general healthy eating. Funding SourcesThis research was funded by Philips Research China, Philips (China) Investment Co., Ltd., Shanghai, China. Supporting Tables, Images and/or Graphs▪▪▪