Postpartum Disease Research Articles

Relationship between preterm birth and post-partum periodontal maternal status: a hospital-based Romanian study.

This cross-sectional case-control study of post-partum women aimed to estimate whether maternal periodontitis was a predictive contributor to preterm birth and to identify other risk factors associated with preterm birth in our target population. The case group included women who delivered preterm (74 cases) and the control group included women who had a normal term delivery (120 controls). Medical records, a 16-item questionnaire, and a full-mouth periodontal examination were used to collect information about socio-demographic characteristics, general health problems, birth-related information, behavioral factors and periodontal status. Logistic regression analysis was used to estimate the strength of the relationship between predictors and the categorical outcome variable, preterm birth. The bivariate analysis revealed the significant associations between preterm birth and socio-demographic factors (educational level, p = 0.003), antepartum smoking habit (p = 0.001) and birth weight lower than 2500g (p < 0.001). The multivariate analysis highlighted that the presence of post-partum maternal periodontitis and its severity remained independent risk factors of preterm birth in the presence of antepartum smoking habit and route of delivery [adjusted OR 2.26, 95% CI (1.06; 4.82), respectively, OR 3.46, 95% CI (1.08; 11.15)]. Post-partum maternal periodontal disease and its severity might, in part, be considered as contributor to preterm deliveries before 37weeks of gestation.

The association of circulating prepartum metabolites, minerals, cytokines and hormones with postpartum health status in dairy cattle

The objectives were to evaluate differences in circulating prepartum metabolites, minerals, cytokines and hormones based on postpartum disease category and determine critical circulating concentrations of prepartum analytes associated with postpartum disease in 229 cattle from 11 commercial dairies in Alberta, Canada. Blood was collected at 8.8 ± 2.1 d prepartum and analyzed for a wide array of analytes. Cattle were categorized as healthy (n = 76) or as having inflammatory (INF; n = 28), metabolic (MET; n = 34) or inflammatory and metabolic (INFMET; n = 91) postpartum diseases. The prepartum circulating concentrations of Cu were lesser (0.84 vs. 0.90 μg/mL; P = 0.02) and concentrations of Mo (19.1 vs. 16.5 ng/mL; P = 0.04) and NEFA (0.27 vs. 0.18 mmol/L; P = 0.01) were greater in INFMET cattle compared with healthy cattle. The critical threshold for Cu, Mo and NEFA prepartum concentration that predicted INFMET was ≤ 0.81 μg/mL (sensitivity 45.5% and specificity 74.3%), ≥ 9.91 ng/mL (sensitivity 70.0% and specificity 52.7%) and ≥ 0.19 mmol/L (sensitivity 62.2% and specificity 79.7%), respectively. Regardless of differences in the prepartum circulating concentrations of Cu, Mo and NEFA among healthy cattle and those with postpartum disease, the use of these analytes to predict the incidence of postpartum diseases was limited.

Parameters for Concentrations of Sows’ Gonadal Steroids in Predicting Inflammatory Processes in the Reproductive Tract

The objective of the survey is to reveal the effects of a dysfunctional relationship between the gonadal steroids (progesterone and 17β-estradiol) on occurrence of inflammatory diseases (endometriosis and metritis-mastitis-agalactia) in sows in the postpartum period. The surveys were carried out with 27 sows at 106–107 days of gestation, measuring the concentrations of progesterone and 17β-estradiol on the seventh, fifth, third, and first days before the expected farrowing date and during parturition. The subsequent retrospective analysis of the results of the collected measurements was performed depending on the course of the postpartum period in sows. It was ascertained that the progesterone blood concentrations the 3 days before the farrowing date and during parturition in sows affected with postpartum diseases were higher by 48.7 (p < 0.01) and 31.1% (p < 0.05), respectively, than that in the animals that remained healthy after farrowing, while the estradiol concentrations were lower by 10.1 and 21.0%, respectively. In addition, progesterone-to-estradiol ratio variables were 1.6-fold (p < 0.01) and 1.7-fold (p < 0.01) higher, respectively. This allows for the consideration that a dysfunctional relationship between the hormones in sows at the end of gestation and during parturition can be one of the factors causing the pathological processes of an inflammatory type in the postpartum period. In addition, it is eligible to apply the progesterone-to-estradiol ratio as a criterion for predicting the risk for disease incidence.

Evaluation of production parameters and health of dairy cows treated with pegbovigrastim in the transition period

The aim of this study was to evaluate the effects of pegbovigrastim injection (Imrestor, Elanco Animal Health, Greenfield, IN) on production parameters and postpartum disease occurrence (retained placenta, metritis, displaced abomasum, and clinical mastitis) in dairy cows. Study cows (n = 270) were blocked by parity group (multiparous or primiparous) and randomly assigned to control (CON, n = 144) or pegbovigrastim treatment (IMR, n = 126). Ten ± 4 days before expected calving and again at calving, IMR cows received 2.7 mL of Imrestor and CON cows received 2.7 mL of 0.9 % saline. Milk yield, fat, protein, lactose, solids nonfat (SNF) percent, and somatic cell count (SCC), body condition, hygiene, and lameness were evaluated weekly. Animals were evaluated for metritis twice weekly through rectal temperature, palpation, and uterine discharge evaluation until 30 days in milk. Farm personnel recorded other postpartum diseases. The MIXED procedure of SAS was used to evaluate milk composition and milk yields were analyzed as repeated measures in time with block, treatment, calving month, and lactation week included in mixed models. The GLIMMIX procedure was used to evaluate mastitis and metritis occurrence. Variables entered a model if P ≤ 0.10 when screened individually. Variables with P ≤ 0.10 were kept in the final model. Milk yield, fat, protein, lactose, SNF, and log of SCC were not significantly affected by treatment. Clinical mastitis occurrence did not differ between treatments, but only 17 cases were detected. Compared to CON, IMR treated cows had increased clinical metritis incidence, and were 2.46 times more likely to develop the disease. No difference was observed on puerperal metritis ocurrence. Cows given pegbovigrastim injections had increased odds of developing clinical signs of metritis, but no negative effects on milk production or composition were observed.

In vivo model to study the impact of genetic variation on clinical outcome of mastitis in uniparous dairy\xa0cows

BackgroundIn dairy herds, mastitis causes detrimental economic losses. Genetic selection offers a sustainable tool to select animals with reduced susceptibility towards postpartum diseases. Studying underlying mechanisms is important to assess the physiological processes that cause differences between selected haplotypes. Therefore, the objective of this study was to establish an in vivo infection model to study the impact of selecting for alternative paternal haplotypes in a particular genomic region on cattle chromosome 18 for mastitis susceptibility under defined conditions in uniparous dairy cows.ResultsAt the start of pathogen challenge, no significant differences between the favorable (Q) and unfavorable (q) haplotypes were detected. Intramammary infection (IMI) with Staphylococcus aureus 1027 (S. aureus, n = 24, 96 h) or Escherichia coli 1303 (E. coli, n = 12, 24 h) was successfully induced in all uniparous cows. This finding was confirmed by clinical signs of mastitis and repeated recovery of the respective pathogen from milk samples of challenged quarters in each animal. After S. aureus challenge, Q-uniparous cows showed lower somatic cell counts 24 h and 36 h after challenge (P < 0.05), lower bacterial shedding in milk 12 h after challenge (P < 0.01) and a minor decrease in total milk yield 12 h and 24 h after challenge (P < 0.01) compared to q-uniparous cows.ConclusionAn in vivo infection model to study the impact of genetic selection for mastitis susceptibility under defined conditions in uniparous dairy cows was successfully established and revealed significant differences between the two genetically selected haplotype groups. This result might explain their differences in susceptibility towards IMI. These clinical findings form the basis for further in-depth molecular analysis to clarify the underlying genetic mechanisms for mastitis resistance.

Immunological adaptations in pregnancy that modulate rheumatoid arthritis disease activity.

During pregnancy, the fetus that grows within the maternal uterus is not rejected bythe maternal immune system. To enable both tolerance towards the fetus and defence againstpathogens, modifications of the maternal immune system occur during gestation. Thesemodifications are able to bring about a natural improvement in disease activity of some autoimmune diseases, such as rheumatoid arthritis (RA). Various mechanisms of the immune system contribute to the phenomenon of pregnancy-related improvement of RA, and the cessation of these immunomodulatory mechanisms after delivery correlates with postpartum disease flare. HLA disparity between mother and fetus, glycosylation of IgG, immunoregulatory pathways, and alterations in innate and adaptive immune cells and their cytokines have important roles in pregnancy and in pregnancy-related amelioration of RA.

Preventing postpartum uterine disease in dairy cattle depends on avoiding, tolerating and resisting pathogenic bacteria

Up to forty percent of dairy cows develop metritis or endometritis when pathogenic bacteria infect the uterus after parturition. However, resilient cows remain healthy even when exposed to the same pathogens. Here, we provide a perspective on the mechanisms that dairy cows use to prevent postpartum uterine disease. We suggest that resilient cows prevent the development of uterine disease using the three complementary defensive strategies of avoiding, tolerating and resisting infection with pathogenic bacteria. Avoidance maintains health by limiting the exposure to pathogens. Avoidance mechanisms include intrinsic behaviors to reduce the risk of infection by avoiding pathogens or infected animals, perhaps signaled by the fetid odor of uterine disease. Tolerance improves health by limiting the tissue damage caused by the pathogens. Tolerance mechanisms include neutralizing bacterial toxins, protecting cells against damage, enhancing tissue repair, and reprogramming metabolism. Resistance improves health by limiting the pathogen burden. Resistance mechanisms include inflammation driven by innate immunity and adaptive immunity, with the aim of killing and eliminating pathogenic bacteria. Farmers can also help cows prevent the development of postpartum uterine disease by avoiding trauma to the genital tract, reducing stress, and feeding animals appropriately during the transition period. Understanding the mechanisms of avoidance, tolerance and resistance to pathogens will inform strategies to generate resilient animals and prevent uterine disease.

Incidence and Predictors of Flares in the Postpartum Year Among Women With Inflammatory Bowel Disease.

The postpartum period is marked by physiological and psychological stresses that may impact activity in inflammatory bowel disease. The predictors and outcomes of disease activity during this period have not been well characterized. We performed a retrospective review of inflammatory bowel disease patients who underwent successful pregnancy and live birth at 2 referral institutions. Data on patient and disease factors including disease activity before and during pregnancy were abstracted from the medical records. We noted whether therapy was dose-reduced or stopped during pregnancy at each trimester and after delivery. Multivariable logistic regression of independent predictors of postpartum flare was performed, adjusting for relevant covariates. We identified a total of 206 eligible women (mean age, 33.2 years). Of these, 97 (47%) had a diagnosis of Crohn's disease, whereas the remainder had ulcerative colitis. Nearly half the women delivered vaginally (53%), and the rest delivered by Caesarean section (47%). In the entire cohort, 65 (31.6%) experienced a postpartum flare within the year after delivery. In multivariable analysis, development of a postpartum flare was predicted by disease activity during the third trimester (odds ratio [OR], 6.27; 95% confidence interval [CI], 2.81-17.27), therapy de-escalation during pregnancy (OR, 3.00; 95% CI, 1.03-8.68), and therapy de-escalation after pregnancy (OR, 4.43; 95% CI, 1.55-12.65). Postpartum disease flare was not related to disease type, duration of disease, or mode of childbirth. One-third of women with inflammatory bowel disease may experience disease flare during the postpartum year. Continued optimization of therapy before, during, and after pregnancy is essential to prevent this morbidity.

Changes in oxidative stress parameters in healthy and diseased Holstein cows during the transition period in Yamagata Prefecture, Japan

We investigated changes in oxidative stress markers during the transition period in healthy Holstein cows and those with postpartum diseases. Transition control (TC) Holstein cows (n=9) were evaluated for longitudinal changes during the transition period and postpartum diseased (PD) cows with ketosis (n=10), abomasal displacement (n=9), and acute mastitis (n=10) were evaluated in comparison to control cows (n=10). In the TC group, blood samples were collected at 2 weeks prepartum and at 1, 2, 4, 6, and 8 weeks postpartum. Milk yield and composition were measured at 2 and 4 weeks postpartum. In the PD group, blood samples were collected at the first day of examination during the 60 days postpartum. Peripheral oxidative stress parameters (malondialdehyde, MDA; potential antioxidant capacity, PAO; and glutathione peroxidase) were measured, and biochemical analyses were performed. In the TC group, MDA increased significantly postpartum and was correlated with milk yield, blood glucose (Glu), free fatty acid (FFA), β-hydroxybutyric acid (BHB), and aspartate aminotransferase. Compared to the control cows, PD cows with ketosis had significantly higher MDA and significantly lower PAO. Moreover, MDA was significantly correlated with Glu, FFA, and BHB. Postpartum increase in MDA might interact with milk yield and Glu, FFA, and BHB in the TC cows, and postpartum diseases, especially ketosis, might signify its increase and interaction with Glu, FFA, and BHB.

Serum metabolites and body condition score associated with metritis, endometritis, ketosis, and mastitis in Holstein cows

Abstract: The objective of this work was to identify the serum metabolites and the body condition score (BCS) of Holstein cows associated with the occurrence of periparturient diseases, when under heat stress. Blood samples were collected from 181 cows one week after calving, and the BCS was recorded at calving. Cows with β-hydroxybutyrate (BHBA) concentration ≥ 0.8 mmol L-1 were 2.2 times more likely to develop metritis and 4.4 times more likely to develop clinical endometritis (CE). Cows with serum creatinine levels ≥ 2.0 mg dL-1 showed 2.2 and 4.5 greater risk (p ≤ 0.05) of suffering from metritis and CE, respectively, during the current lactation. The odds of metritis and CE occurrence were 2.7 and 4.6 times greater (p &lt; 0.01) in cows with serum total protein (TP) ≥ 5.0 mg dL-1. Cows with serum glucose levels ≤ 70 mg dL-1 and non-esterified fatty acids (NEFAs) ≥ 0.5 had 9.4 and 8.8 times greater odds of developing clinical ketosis, respectively, than cows with lower glucose and NEFA blood levels. The strategic use of metabolic tests to monitor transition Holstein cows, aiming to prevent some postpartum diseases due to heat stress, should focus on blood BHBA, NEFAs, glucose, creatinine, and TP. Additionally, the BCS ≤ 3.2 should be avoided to prevent endometritis.

On some problems of modern intensive animal husbandry

Metabolism violations are one of the most acute problems of industrial husbandry farming. Ketosis of cows remains an urgent problem. The material for the study was highly productive cows of the first and second lactations on 1-14 days after calving, weighing 480-630 kg. Animals suffering from metabolic subclinical ketosis were divided into two groups (71 heads in each group). Clinical studies were performed by testing the amount of β-hydroxybutyrate and analyzing data in Dairy Comp 305. The enterprise protocols were used to treat the animals. The effectiveness of treatment was evaluated every day; on the second day, 46.5% of cows recovered in the first group, 38.0% - in the second group, and 53.5% and 62% of cows in each group recovered on the third day. In animals with metabolic disorders, in particular, ketosis, the genetic lactation potential is not fully used which decreases profitability of the farm. Daily well-structured work aimed at preventing and treating postpartum diseases that decrease dry matter intake can reduce the risk of secondary ketosis. To reduce the risks of primary (metabolic) ketosis, it is necessary to follow the feeding regimes in all technological groups of animals and form them from animals with the same hierarchical status.

Dependency of the dıseases occurred after delıvery from the age and number of lactatıon among cows ın Guba-Khachmaz regıon of Azerbaıjan Republıc

High productivity breed cows are delivered to the Republic recently for intensive development of livestock. Subsidies are allocated to farmers, modern type livestock complexes are constructed by state. But, although all conducted works, there are several diseases which prevent the development of livestock. The leading one of such diseases are diseases occurred after delivery among breed cows. Along with various factors, special attention involves the age of animals and number of lactation in occurrence of such diseases. In order to study the effect of livestock age and the number of lactations on the occurrence of diseases, a study was conducted on highly productive thoroughbred and local cows of the Guba-Khachmaz region. During the study period, on the farms located in the Guba, Gusar, Khachmaz regions of the Guba-Khachmaz economic region, 4826 cattle were examined. Thus, in the Guba region, 828 heads of cows were examined: 130 heads at the Shahin farm, 141 heads at the Guneshli farm, 438 heads at the Pirvahid farm, and 119 heads at private farms. In the Gusar region, a total of 3696 cattle were examined: 3540 animals in the Shahdag livestock complex, 119 cows on private farms. In the Khachmaz region, 302 heads of cattle were examined: on the Isaev farm – 170 heads, on private farms - 132 heads of cows. Metritis was observed in 361cows from 4826 cows between 3–8 ages and older, as well as passing lactation for 6 and more times (7.5%), mastitis in 278 of 4826 cows (5.8%), latency delay in 106 of 4826 cows (2.2%), and birth paresis in 42 of 4826 cows (0.9%) in Guba – Khachmaz natural – economic geographic region. The results of the study show that as the age of cows and the number of lactations increase, their sensitivity to postpartum diseases increases.

Graves' Disease and the Post-partum Period: An Intriguing Relationship.

The post-partum period is an immunologically peculiar period in a woman's life. Indeed, most of the pregnancy-related immune changes gradually revert in the 12 months following delivery. Although the post-partum period has long been identified as a period of aggravation of autoimmune thyroid diseases, most of the currently available studies took into account the relationship between post-partum and autoimmune thyroiditis. More recently, the potential repercussions of the post-partum period on Graves' disease were also taken into account. The present mini review will briefly overview the most recent advances in our knowledge of the immunology of the post-partum period in relation with the potential repercussions on the clinical course of Graves' disease. Moreover, some peculiar aspects of post-partum Graves' disease in terms of clinical and biochemical presentation, diagnostic challenges, and specific therapeutic considerations also taking into account the recommendation of the latest clinical guidelines on the management of thyroid diseases in pregnancy will be overviewed.

Relationship between changes in prepartum rumen fill score and peripartum metabolic status, postpartum disease, and recovery of reproductive function in dairy cows

Relationship between changes in prepartum rumen fill score and peripartum metabolic status, postpartum disease, and recovery of reproductive function in dairy cows

Preterm delivery is associated with long-term risk of maternal renal disease

Abstract Background Preterm delivery is an independent risk factor for maternal cardiovascular disease. Little is known about the association between preterm delivery and maternal renal function, and whether any association is independent of preeclampsia or intra-uterine growth restriction. This study aimed to examine the association between gestational age and long-term maternal chronic kidney disease (CKD) and end-stage kidney disease (ESKD). Methods Using data from the Swedish Medical Birth Register, singleton live births from 1973-2012 were identified and linked to data from the Swedish Renal Register and National Patient Register (up to 2013). Women with pre-pregnancy CKD/ESKD, cardiovascular disease, diabetes, hypertension, systemic lupus erythematosus were excluded. Gestational age at delivery was the main exposure, treated as a time-dependent variable. Primary outcomes were maternal CKD or ESKD. Cox regression was used, adjusting for maternal age, year of delivery, country of origin, education, parity, interpregnancy interval, smoking, BMI, gestational diabetes. Models were stratified by exposure to preeclampsia or small for gestational age (SGA). Results There were 3,847,694 pregnancies among 1,990,273 unique women. Nine percent of women (n = 172,915) had at least one preterm delivery (&amp;lt;37 weeks). Exposure to preterm delivery was associated with higher risk of CKD (aHR 1.48, 95%CI 1.41-1.54) and ESKD (aHR 2.52, 95%CI 2.17-2.92). Earlier gestational age at delivery was associated with increasing risk of CKD and ESKD. Women with spontaneous preterm delivery were at higher risk of CKD (vs. normal term, aHR 1.33, 95% CI 1.26-1.40) and ESKD (aHR 2.02, 95%CI 1.69-2.40) independently of preeclampsia/SGA. Associations persisted after excluding women who developed postpartum cardiovascular disease, hypertension or diabetes. Conclusions Women who gave birth at earlier gestation were at higher risk of later CKD and ESKD. This association persisted independently of preeclampsia and SGA. Key messages Preterm delivery is an independent predictor of long-term maternal renal disease. Obstetric history should be considered as part of overall risk stratification for chronic kidney disease in women.

Neutrophil β-defensin gene expression of postpartum dairy cows is altered by prepartum dietary cation-anion difference

The objective of this study was to evaluate expression of a cluster of genes encoding β-defensin antimicrobial peptides in neutrophils of postpartum cows in relation to prepartum dietary cation-anion difference (DCAD), vitamin D, and postpartum disease. Pregnant dry Holstein cows (28 nulliparous and 51 parous) at 255 d gestation were blocked by parity and randomly assigned to 4 prepartum diets of positive (+130 mEq/kg) or negative (-130 mEq/kg) DCAD and either 3 mg vitamin D3 or 3 mg of 25-hydroxyvitamin D3 per 11 kg of dry matter/d. Treatment diets were fed from 255 d of gestation until calving. Peripheral blood neutrophils of 35 parous cows were collected at 0 and 3 d after calving and stimulated with 0 or 100 ng/mL of lipopolysaccharide (LPS). Furthermore, serum Ca and incidences of postpartum diseases were recorded for all cows. The mRNA transcripts of β-defensin genes were quantified by real-time PCR, and data were analyzed with a general linear mixed model to test for fixed effects and interactions of day, level of DCAD, source of vitamin D, and incidence of disease. Effects of DCAD and vitamin D on neutrophil oxidative burst and phagocytosis were previously reported but were analyzed for effects of disease in the present study. Transcripts for DEFB1, DEFB3, DEFB4, DEFB5, DEFB7, DEFB10, and lingual antimicrobial peptide (LAP) in neutrophils were upregulated by LPS at 0 d but not at 3 d. Transcripts for DEFB4 and DEFB7 in LPS-stimulated neutrophils were greater in cows fed negative DCAD diets compared with positive DCAD. Source of vitamin D (vitamin D3 vs. 25-hydroxyvitamin D3) did not affect expression of β-defensins in neutrophils. Cows with postpartum subclinical hypocalcemia (serum Ca <2.0 mM) had decreased DEFB3, DEFB4, DEFB6, DEFB7, DEFB10, and LAP expression in LPS-stimulated neutrophils compared with cows that did not experience subclinical hypocalcemia. Likewise, DEFB4, DEFB6, DEFB7, DEFB10, and LAP in LPS-stimulated neutrophils at 3 d postpartum were positively associated with serum Ca at 0 d postpartum. Transcripts for DEFB7, DEFB10 and LAP also were less abundant in neutrophils from cows with metritis compared with healthy cows. In conclusion, feeding a prepartum negative DCAD to improve postpartum serum Ca resulted in greater neutrophil β-defensin expression, and greater neutrophil β-defensin expression was positively associated with postpartum health.

Long-term effects of postpartum clinical disease on milk production, reproduction, and culling of dairy cows

Two retrospective studies examining data of 7,500 lactating cows from a single herd were performed with the objective of evaluating the long-term effects of clinical disease during the early postpartum period on milk production, reproduction, and culling of dairy cows through 305 days in milk (DIM). In the first study, data regarding health, milk production, reproduction, and culling of 5,085 cows were summarized. Cows were classified according to incidence of clinical problem (metritis, mastitis, lameness, digestive problem, or respiratory problem) during the first 21 DIM (ClinD21). During 305 d of lactation, cows that had ClinD21 produced, on average, 410 kg less milk, 17 kg less fat, and 12 kg less protein compared with cows that did not have ClinD21 (NoClinD21). Although the interval to first breeding was not different between groups of interest, pregnancy rate through 305 DIM was lower in cows that had ClinD21 [adjusted hazard ratio (AHR) = 0.81]. When individual breedings were analyzed, cows that had ClinD21 presented lower rates of pregnancy per breeding for breedings performed before 150 DIM, reduced numbers of calving per breeding for breedings performed before 200 DIM, and greater number of pregnancy losses for all breedings performed through 305 DIM. The rate of culling from calving through 305 DIM was higher in cows that had a single ClinD21 (AHR = 1.79) and in cows that had multiple ClinD21 (AHR = 3.06), which resulted in a greater proportion of cows leaving the herd by 305 DIM (NoClinD21 = 22.6%; single ClinD21 = 35.7%; multiple ClinD21 = 53.8%). In the second study, data regarding postpartum health and 305-d yields of milk, fat, and protein were collected from 2,415 primiparous cows that had genomic testing information. Genomic estimated breeding values (EBV) were used to predict 305-d yields of milk, fat, and protein. Genomic EBV and predicted yields of milk, fat, and protein did not differ between cows that had ClinD21 and those that did not have ClinD21. In contrast, the observed 305-d yields of milk, fat, and protein were reduced by 345, 10, and 10 kg, respectively, in cows that had ClinD21 compared with cows that did not have ClinD21. We conclude that clinical disease diagnosed and treated during the first 21 DIM has long-term effects on lactation performance, reproduction, and culling of dairy cows, which contribute to detrimental consequences of health problems on sustainability of dairy herds. Replication of our studies in multiple herds will be important to confirm our findings in a larger population.

Dependency of the diseases occurred after delivery from the age and number of lactation among cows in Guba-Khachmaz region of Azerbaijan Republic

High productivity breed cows are delivered to the Republic recently for intensive development of livestock. Subsidies are allocated to farmers, modern type livestock complexes are constructed by state. But, although all conducted works, there are several diseases which prevent the development of livestock. The leading one of such diseases are diseases occurred after delivery among breed cows. Along with various factors, special attention involves the age of animals and number of lactation in occurrence of such diseases. Metritis was observed in 361cows from 4826 cows between 3-8 ages and older, as well as passing lactation for 6 and more times (7.5%), mastitis in 278 of 4826 cows (5.8%), latency delay in 106 of 4826 cows (2.2%), and birth paresis in 42 of 4826 cows (0.9%) in Guba – Khachmaz natural – economic geographic region. In the Guba-Khachmaz region, among the three-year-old cows during the first lactation, infections with metritis amounted to 4.7%, mastitis – 3.1%, detention of the sequence – 0.7%, birth paresis - 0.3%; Among four-year-old cows during the second lactation, infections by metritis amounted to 5.1%, mastitis – 3.5%, detaining the sequence – 1.6%, generic paresis - 0.4%; among five-year-old cows during the third period of lactation: 6.3% metritis, 5.8% mastitis, 2.3% post-mortem, generic paresis – 0.5%; among six-year-old cows during the fourth lactation: 8.2% metritis, 6.2% mastitis, 2.6% post-sedentation, 0.7% birth paresis; Among the seven-year-old cows during the fifth lactation: metritis 9.0%, mastitis 7.2%, post-traumatic delay 2.7%, birth paresis 1.5%; among cows at the age of 8 years and older who have undergone 6 or more lactations: metritis 10.7%, mastitis 8.9%, postural detention 3.4%, generic paresis 1.7%. The results of the study show that with increasing age of cows and the number of lactations, their sensitivity to postpartum diseases increases.

Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows

This study evaluated and compared infrared thermography (IRT) and rectal temperature (RT) as screening tests to identify sick transition dairy cows. Holstein cows (n = 72; 42 primiparous) had RT and IRT temperatures taken daily from 1 to 12 days in milk (DIM). Health examinations were performed daily to diagnose retained fetal membrane, milk fever and metritis, and blood was analyzed for β-hydroxybutyrate at 6 and 9 DIM to diagnose ketosis. Plasma concentrations of cortisol, interleukin-6, tumor necrosis factor α and serum amyloid A at 3, 6, 9 and 12 DIM were included as additional indicators of illness. Cows were categorized as true sick if clinically diagnosed with an illness, or if at least 2 blood parameters were above the normal range. Diagnostic test performances for RT and IRT variables were determined for each variable at a test referent value that provided the highest Youden's (J) index. The best performing screening test depended on the definition of true sickness. In general, the J index for RT was 0.15–0.17 whereas the highest J index for the IRT variables was 0.22 for the mean eye temperature and 0.19 for the mean cheek temperature. Infrared thermography was at least comparable to RT and some IRT variables performed better as a screening tests than RT. Future studies into the automation of IRT for surveillance of early postpartum diseases is warranted.

Pharmacological mechanism of Shenghua Decoction in treatment of abdominal pain based on network pharmacology

The Shenghua Decoction recorded in Fu Qing Zhu's Gynaecology,is a commonly used postpartum prescription,widely used in treating postpartum and gynecological diseases. However,its mechanism of action in treating lower abdominal pain remains unclear. In this paper,network pharmacology was used to explore the mechanism of Shenghua Decoction in the treatment of lower abdomen pain,so as to provide data support for better clinical application of Shenghua Decoction. The drug targets of lower abdominal pain and Shenghua Decoction were retrieved in SymMap. String and Cytoscape were adopted for enrichment analysis to construct the disease-drug-target biological network. Relevant gene search results showed that there were 400 targets in Shenghua Decoction,11 of which coincided with the disease genes of lower abdomen pain. In String analysis,18 gene interactions were obtained. Gene modularizationbased analysis results indicated that one module containing six genes was obtained after modularization processing. Furthermore,there were 170 enrichment results of biological process,2 enrichment results of molecular function and 30 enrichment results of KEGG pathways in String enrichment analysis. Shenghua Decoction may play a role in treating lower abdomen pain through neuro-endocrine-immune,metabolism and other means. Its mechanism may be achieved by accelerating the repair and growth of endometrial tissue cells,improving microcirculation,promoting endometrial cell renewal and inflammation subsidence,and accelerating uterine involution; at the same time,it can regulate the autoimmunity,regulate and control the function of some natural immune cells in the process of antiinfection by using signaling pathway,supplement the vital energy,and induce elimination of pathogens from the body,thereby achieving the effect of treating lower abdomen pain.