Aim: The study was conducted as a descriptive study in order to determine mothers’ postpartum comfort levels and the affecting factors. Materials and Method: The sample of the study consisted of 526 postpartum mothers in two hospitals in a province of Turkey and who accepted to participate in the study. Data were collected using face-to-face interview method by Personal Information Form and Postpartum Comfort Scale (PCS). Results: In this study, it was detemined that the mean score of the mothers from PCS was 122.88±15.02, 46.27±7.66 in physical comfort, 43.48±5.10 in psychospritual comfort and 33.09±6.59 in sociocultural comfort, and the mothers’ comfort was found to be at moderate level. Physical, psychospiritual, sociocultural comfort and total mean scores of the mothers who planned their pregnancy, who described health professionals’ behaviors as quite gentle, who had education during hospitalization, who were satisfied with the care, who expressed their general health condition as very good and who felt relieved thanks to postpartum care were significantly higher than the other groups (p<0.05). Conclusion: It was determined that planning of pregnancy, perception of birth, general health status, behaviors of health professionals, having education during hospitalization and satisfaction with the care affected the mothers’ comfort levels. Mothers should be informed and supported during the prenatal, delivery and postpartum periods, delivery services should be arranged in a way to increase comfort and health professionals should behave gently to mothers and their families.