Adequate pretreatment and proper donor nephrectomy are important prerequisites for successful renal transplantation with considerable impact on initial renal function and potential postoperative surgical and urological complications. The physiological function of the donor kidneys must be maintained until devascularization. The technique of donor nephrectomy should guarantee minimal warm ischemia and preservation of the renal and ureteral vascular supply. Moreover, this procedure should be standardized, as it is also easily applicable by nontransplant surgeons and urologists in hospitals procuring donor kidneys for transplant centers.