The purpose of this study was to reveal the correlation between the size of the native anterior cruciate ligament (ACL) footprint and the area of commonly used autografts using cadaveric knees. Twenty-Four non-paired human cadaver knees were used. The size of the femoral and tibial ACL footprints, length of Blumensaat's line, and the height and area of the lateral wall of the femoral intercondylar notch were photographed and measured with Image J software (National Institution of Health). Simulating an semitendinosus tendon (ST) graft, the ST was cut in half. The bigger half was regarded as the antero-medial (AM) bundle, and the remaining half was regarded as the postero-lateral (PL) bundle. Simulating an semitendinosus and gracilis (ST-G) graft, the bigger half of the ST and G was regarded as the AM bundle, and the smaller half of the ST was regarded as the PL bundle. Each graft diameter was measured, and the graft area was calculated. Simulating a bone-patella tendon-bone (BPTB) graft, a 10-mm wide BPTB graft was harvested and the area calculated. The sizes of the native femoral and tibial ACL footprints were 72.3 ± 24.4 and 134.1 ± 32.4 mm(2), respectively. The length of Blumensaat's line, and the height and area of the lateral wall of the femoral intercondylar notch were 29.5 ± 2.5 mm, 17.7 ± 2.3 mm, and 400.9 ± 62.6 mm(2), respectively. The average areas of the ST, ST-G, and BPTB graft were 52.7 ± 6.3, 64.7 ± 7.6, and 37.1 ± 7.5 mm(2). Both the height and the area of the lateral wall of the femoral intercondylar notch were significantly correlated with the femoral size of the ACL footprint (p = 0.007 and 0.008, respectively). However, no significant correlation was observed between ACL footprint size and autograft size. No significant correlation was observed between autograft size and the size of the lateral wall of the femoral intercondylar notch. In ACL reconstruction, if the reconstructed ACL size is determined by the harvested autograft size alone, native ACL size and anatomy are unlikely to be reproduced.