The purpose of this study was to clarify the normal relationships between anterior components of lateral occlusion and muscle activity of 12 healthy subjects. The location (LO) and area (AO) of occlusal contacts were measured by black silicone methods, frontal (FA) and horizontal (HA) angles were also measured by LED jaw tracking device at canine's edge to edge to lateral position (ED-LP) as an anterior component of lateral occlusion. Muscle activity of masseter muscle (Mm), anterior temporal muscle (Ta), posterior temporal muscle (Tp) were recorded bilaterally by bipolar electrodes during maximal voluntary clenching (MVC) at ED-LP.The muscle activity during MVC was affected by anterior components, and the most effective muscle was the working-side Ta and Tp. In the case that frontal teeth with canine restricted on working-side LO, the reduction of muscle activity was observed as the reduction of AO or increase of FA. However, if the molar teeth were included on working-side LO, AO did not affect the muscle activity which could be decreased only by the increase of FA. If the working-side contacts were established, the balancing-side contacts did not affect the muscle activity of Mm, Ta, and Tp during MVC.