The purpose of this study is to evaluate the influence of the matrix system on proximal contact tightness (PCT) of posterior composite resin restorations. Standardized class II cavities on 180 first lower molar dentiform model teeth (Frasaco GmbH, Tettnang, Germany) were prepared. Three groups were formed considering the matrix system: Group-IM: Ivory matrix (Hahnenkratt GmbH, Königsbach-Stein, Germany), Group-OM: Omni matrix (Ultradent, South Jordan, UT, USA), and Group-PM: Palodent V3 sectional matrix (Dentsply, Charlotte, NC, USA). Teeth were restored with resin composite mounted in a manikin head to simulate the clinical environment. Proximal contact tightness (PCT) was measured using a custom-made portable dental pressure meter (PDPM), and the validation of the PCT results was performed with a histogram analysis acquired from bite-wing radiography. All data were statistically analyzed by ANOVA and t-test in SPSS software (v.27.0) (p < 0.05). PM group showed statistically tighter contacts on both mesial (PCTm: 228.28 ± 59.17 N) and distal surfaces (PCTd: 254.91 ± 65.69 N) (p > 0.05). Mesial contacts were found to be significantly tighter than distal contacts among all (p < 0.05). According to the histogram results, only in the PM group, the difference between the mesial and distal areas is significant (p < 0.05). Histogram results confirmed that the tightest contact values were achieved in the PM group, followed by the OM and IM groups, respectively (p < 0.05). The use of sectional matrix systems and separation rings is more effective in creating tighter contact than conventional matrix systems. The use of anatomical wedges will help to create a more natural approximal contour and narrow contact area.