Postdoctoral Research Associate Research Articles

First person – Thomas Williams

ABSTRACT First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping researchers promote themselves alongside their papers. Thomas Williams is first author on ‘ Distinct TORC1 signalling branches regulate Adc17 proteasome assembly chaperone expression’, published in JCS. Thomas conducted the research described in this article while a Postdoctoral Research Associate (PDRA) in Dr Adrien Rousseau's lab at the MRC-PPU, School of Life Sciences, University of Dundee, UK. He is now a PDRA in the lab of Prof. Pedro Carvalho at Sir William Dunn School of Pathology, University of Oxford, UK. Thomas is interested in the mechanisms cells use to adapt to changes in their environment, encompassing changes to intracellular signaling, proteome composition and cell physiology.

A Novel Nutritional Therapy Through Lysophosphatidyl Choline DHA on Arsenic Exposed Dams and Pups, Postdoctoral Research Associate

A Novel Nutritional Therapy Through Lysophosphatidyl Choline DHA on Arsenic Exposed Dams and Pups, Postdoctoral Research Associate

ECR Spotlight – Corbin Rasmussen

ECR Spotlight is a series of interviews with early-career authors from a selection of papers published in Journal of Experimental Biology and aims to promote not only the diversity of early-career researchers (ECRs) working in experimental biology but also the huge variety of animals and physiological systems that are essential for the ‘comparative’ approach. Corbin Rasmussen is an author on ‘ Curvilinear walking elevates fall risk and modulates slip and compensatory step attributes after unconstrained human slips’, published in JEB. Corbin conducted the research described in this article while a PhD student in Nathaniel H. Hunt's lab at the Department of Biomechanics, University of Nebraska at Omaha, USA. Corbin is now a Postdoctoral Research Associate in the lab of Sara A. Myers, at the Department of Biomechanics, University of Nebraska at Omaha, USA, investigating the mechanisms of human balance recovery across commonly encountered walking environments and tasks.

In the Lab: Heterogeneous Catalysis Mediated Interconversion between NAD(P)+ and NAD(P)H Accompanied by Consumption and Generation of Hydrogen

Dr. Wang is a Senior Lecturer in Chemical Engineering at Lancaster University. Prior to this, he was a Lecturer in Chemical Engineering at the University of Aberdeen, Postdoctoral Research Associate at Heriot-Watt University, where he also obtained his PhD (2014). He completed both MSc and BEng studies at Tianjin University. Wang has been the author of over 50 peer-reviewed publications, an editorial board member of Chinese Chemical Letters and his research has mainly been funded by the Engineering and Physical Sciences Research Council (EPSRC), Royal Society, UK Catalysis Hub and industry. Wang’s research interest ranges from reaction engineering, green energy and materials to chemicals, where heterogeneous catalysis is the core discipline. His recent work has focused on the innovative use of heterogeneous catalysts (e.g., supported metals) in enzymatic transformations via cofactor regeneration,1,2 paving the way to a potential new regeneration technology.

Crossfit, SoulCycle, and Evangelical Christianity with Cody Musselman

The Interview brings you in-depth exchanges with scholars working to reframe how we carry out the academic study of religion. In this edition, Michael J. Altman at the University of Alabama sat with Cody Musselman (Postdoctoral Research Associate at the John C. Danforth Center on Religion and Politics at Washington University in St. Louis, PhD Yale 2022) to discuss SoulCycle, CrossFit, and evangelical Christianity in the United States. In so doing, these scholars of religion in America open up numerous possibilities for theorizing the intersections of capitalism, neoliberalism, and religion. This exchange has been excerpted from the 2021 Day Lecture at the University of Alabama.

STAT1 gain of function mutation impairs immune response to viral infections

Abstract The coordinated effort of the immune system in host defense is dependent upon the proper release and interpretation of different cytokine signals. Several examples of monogenic diseases targeting the JAK-STAT pathway reveal there is still a critical need to understand basic principles of cytokine signaling and cytokine output. For example, patients with STAT1 gain-of-function (GOF) mutations exhibit a type 1/IFN-gamma bias that antagonizes a type 3/IL-17 immune response important for controlling fungal infection, yet patients also, paradoxically, exhibit chronic and sometimes lethal viral infections. Using a novel conditional knock in STAT1-GOF mouse model, we demonstrate that STAT1-GOF mice respond poorly to viral infections that are easily controlled by WT mice, such as MCMV and LCMV-Armstrong. High-dimensional flow cytometry and scRNA-seq reveal an impaired NK and CD8 T cell effector response in STAT1-GOF mice that is not simply due to an exhaustion phenotype. Instead, STAT1-GOF mice respond poorly to viral infections due to an unexpected defect in IFN-gamma production early during the innate immune response, leading to a cytokine storm, immunopathology, and an impaired adaptive immune response. scATAC/RNA-seq uncovers altered usage of different STAT complexes in STAT1-GOF mice, leading to a misinterpretation of early innate-stimulating cytokines. These results provide insight into why patients with interferonopathies or elevated interferons respond poorly to viral infections and highlight the importance of properly tuning STAT1 during an immune response. R.L.P. was supported by a Postdoctoral Research Associate (PRAT) fellowship from the National Institute of General Medical Sciences (NIGMS), award number 1Fi2GM137942-01

Hijacking Suppression: Anti-CD40 Converts Regulatory T Cells Into Type I Effectors

Abstract Background: Pancreatic ductal adenocarcinoma (PDA) is an aggressive and heterogeneous cancer that is often refractory to current treatments. We found that a combination of anti-PD1, anti-CTLA-4, and anti-CD40 results in tumor regression. Thus, the aim of our study is to gain mechanistic understanding into this therapeutic combination. To that end, we leverage quantitative high multiplex microscopy, enabling us to decipher the complexities of cellular behaviors, interactions, and phenotypes in an intact TME. Results and Conclusions: In the TME of responsive tumors, our therapy severely depleted regulatory T cells. We found this Treg loss to be ADCC–independent, but anti-CD40–dependent, a surprising result given that anti-CTLA-4 was thought to mediate Treg depletion. Residual Tregs were restricted to the tumor periphery by 48 hours post anti-CD40 administration. We used tamoxifen-inducible Foxp3 lineage tracing mice to label all Foxp3–expressing cells prior to therapy initiation. Strikingly, we found that many of the lineage marked Tregs no longer expressed Foxp3. These ExTregs now had high levels of Tbet and IFNg, and had evidence of cognate antigen recognition, as assessed via imaging of NFAT1 nuclear translocation in situ. Single and combination treatments in the absence of anti-CD40 failed to induce this ExTreg phenomenon. Blockade of MHC-II, IL-12, or IFNg or tumor implantation into Batf3 KO, IL12p40 KO, or IFNg KO mice also ablated this phenomenon. Combined, these data reveal a unique mechanism by which the clinically relevant agonistic anti-CD40 amplifies the anti-tumor immune compartment through the conversion of immunosuppressive Tregs to an effector population within the tumor microenvironment. This work was supported by (1) Intramural Research Program of NIAID, NIH, (2) Postdoctoral Research Associate Training (PRAT) Fellowship of NIGMS, NIH, (3) Parker Institute for Cancer Immunotherapy, and (4) Bench to Bedside Award.

In Memoriam

Peter James (Pete) McNulty was born in 1941 and grew up in New York City where his parents, immigrants from Ireland, had settled. He attended St. Michaels High School and attributed his early interest in science to his high school physics classes. His older sister, also a scientist, had a strong influence as well. Pete received the B.S. degree in physics from Fordham University, Bronx, NY, USA, in 1962 and pursued graduate studies at the State University of New York at Buffalo (SUNY-Buffalo), Buffalo, NY, USA, earning the Ph.D. degree in 1965. He remained at SUNY-Buffalo as a Postdoctoral Research Associate for an additional year, where he met his future wife, Pat. In 1966, Pete and Pat moved to Potsdam, NY, USA, where Pete took an Assistant Professor of Physics position at Clarkson University, eventually becoming a Professor of physics. During their time in the North Country, Pete and Pat raised their two children, Patty and Peter.

FRACTURES: THE IMPACT OF DISCORD, DISINFORMATION, AND DAMAGED DEMOCRACY

On November 23, 2022, Dr. Caroline Orr Bueno, Postdoctoral Research Associate at the University of Maryland, presented Fractures: The Impact of Discord, Disinformation, and Damaged Democracy. The key issues were the difficulty of defining and operationalizing disinformation, the major forms of extremist disinformation and what makes them effective to the human brain, and how disinformation was used by the 2022 Freedom Convoy. Received: 2023-01-14Revised: 2023-01-17

Computational methods in unraveling the mechanism of metallaphotoredox catalysis

Mingbin Yuan obtained his bachelor's degree in chemistry from Sun Yat-sen University and Hong Kong Polytechnic University (joint undergraduate program) in 2017. After that, he went on to pursue a PhD degree in the field of physical organic chemistry and computational chemistry under the mentorship of Prof. Osvaldo Gutierrez. He focused on applying computational tools for accelerating reaction discovery and understanding the controlling factors of reactivity and selectivity in organic and organometallic reactions. After graduating from the University of Maryland in May 2022, he joined the Theoretical Division of Los Alamos National Laboratory as a postdoctoral research associate.

Contributors to this Issue

Other| December 01 2022 Contributors to this Issue Nineteenth-Century Literature (2022) 77 (2-3): 192–193. https://doi.org/10.1525/ncl.2022.77.2-3.192 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Contributors to this Issue. Nineteenth-Century Literature 1 December 2022; 77 (2-3): 192–193. doi: https://doi.org/10.1525/ncl.2022.77.2-3.192 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentNineteenth-Century Literature Search Daniel Jenkin-Smith is a Postdoctoral Research Associate in the Department of English, Languages and Applied Linguistics at Aston University. He is coauthor and coeditor, with Abigail Boucher, of a series of articles for Victorian Review entitled “Bodily Fluids in the Long Nineteenth Century,” published in spring 2019. He has also coauthored, with Abigail Boucher, a chapter on Herman Melville’s Pierre (1852) as a mock-Gothic novel for the forthcoming collection Gothic Melville, edited by Monika Elbert and Jeffrey Weinstock. Currently he is preparing a monograph based on his doctoral thesis, “Bureaucratisation and the Rise of Office Literature: 1810–1900.” Stephanie Kinzinger is a Ph.D. Candidate in the Department of English and Comparative Literature at the University of North Carolina at Chapel Hill. She is finishing a dissertation that uses literary theory and critical game studies to explore how the formal and political possibilities of video... You do not currently have access to this content.

Postdoctoral Research Associate Training Program (NIGMS)

Federal Grants & ContractsVolume 46, Issue 17 p. 7-7 Grant Alerts Postdoctoral Research Associate Training Program (NIGMS) First published: 02 August 2022 https://doi.org/10.1002/fgc.32526Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume46, Issue174 August 2022Pages 7-7 RelatedInformation

Multiplex microscopy reveals unique spatiotemporal effects of cancer immunotherapies

Abstract Pancreatic ductal adenocarcinoma (PDA) is an aggressive and heterogeneous cancer that is often refractory to current treatments. To address this issue, we employ a mouse PDA-derived tumor clone library where individual clones elicit a spectrum of unique immune cell infiltration profiles in the tumor microenvironment (TME). We found that a combination of anti-PD1, anti-CTLA-4, and anti-CD40 results in tumor regression only in tumor clones with a high number of infiltrating T cells. The aim of our study is to gain mechanistic understanding into this therapeutic combination. To that end, we leverage quantitative high multiplex microscopy, enabling us to decipher the complexities of cellular behaviors, interactions, and phenotypes in an intact TME. We found that our therapy distinctively alters the TME of both responsive and non-responsive tumors. Unique to non-responsive tumors, we found discrete myeloid clusters scattered through the TME that increase in frequency and size post therapy. Additionally, we witnessed prominent cellular movement within myeloid clusters using 2-photon intravital imaging. In contrast, in the TME of responsive tumors our therapy severely depleted, reprogrammed, and restricted regulatory T cells to the tumor periphery within 48 hours post anti-CD40 administration. We found this to be ADCC–independent, but anti-CD40–dependent, with the mechanistic factors driving the fate of these cells under detailed investigation. Combined, these data reveal mechanistic insight into anti-CD40 combination therapies and provide a platform for investigating the factors driving the formation and maintenance of an immunosuppressive TME. This work was supported by (1) Intramural Research Program of NIAID, NIH, (2) Postdoctoral Research Associate Training (PRAT) Fellowship of NIGMS, NIH, (3) Parker Institute for Cancer Immunotherapy, and (4) Bench to Bedside Award.

Echoes, and: Mist

Echoes, and: Mist Romeo Oriogun (bio) Echoes In Boston, I walked the night.From blackness I beheld the salvationof dreams. I have moved. In rural Oregon, the plants spoke.Red Alders, White Oaks stood still,two geldings ran aroundin a small enclosure. Tiny creatures ran underneathmy foot as I walked the wooden bridgethat separated small pondfrom paths leading to a farmhouse. So much hope ran through this familiarspace. Across the bridge, in the small pond,I saw the algae of my childhood. I trembled,I fell to the ground, it was August. I heard the earthworms saying,we are of this world,this world is ours only,our great bounty, in it you live. In all your yearning for home,you can only tread this pondfrom here to there, the beginningof a narrowing path. [End Page 44] Mist I entered a train stationand the air trembled, swallows searchedfor rooms in the brief wonder of flight. The graffiti on the pillars spoke of nothing.The day ran into the nightlike a child who having been harmedby a stranger must seek refugein the darkness of a closed room. I wanted the world to be still, the cloudsshaped like bicycles to stop moving.I wanted the girl, whose black clothes spokeof mourning, to look at me. The world has happened, I had left home,and from afar I could imagine the houseson the hill of my birth, the houses leaninginto each other like old souls fallinginto the slow embrace of pity. The doors to my country have been closedto me, the canvas of my mind torn into shreds.What home I knew is now the abode of hawks,and the falconer whose falcon escapedinto the wild must begin to think about freedom. In every mirror, I said to myself, I did not dietoday; the world kept moving. At my departure I said goodbye, I said welcome,I said nothing, and the city turned its back at me, [End Page 45] becoming a stranger I will walk past someday,in a country beyond rivers, then turnto wonder, where have I seen this face, this kingdom,where was my life taken from me? [End Page 46] Romeo Oriogun Romeo Oriogun is the author of Sacrament of Bodies, a finalist for the Lambda Award for Gay Poetry. He has received fellowships and support from Ebedi International Writers Residency, Harvard University, Hutchins Center for African and African American Research, Oregon Institute for Creative Research, and the IIE Artist Protection Fund. Winner of the 2017 Brunel International African Poetry Prize and an alumnus of the Iowa Writers' Workshop, he currently lives in Ames where he is a Postdoctoral Research Associate at Iowa State University. Copyright © 2022 University of Nebraska Press

Portraits on the Wall

Portraits on the Wall Tiana Reid (bio) Click for larger view View full resolution Barbara and Beverly. Roxbury, Massachusetts. 1978 © JEB (Joan E. Biren) from Eye to Eye: Portraits of Lesbians published by Anthology Editions (2021). In 1978, photographer and self-described propagandist Joan E. Biren, better known as JEB, took a photo of Barbara and Beverly Smith, two of the founders of the Boston-based Combahee River Collective (CRC). Barbara gazes softly at her twin Beverly, while Beverly looks up, maybe mid-thought, with some papers in her lap. Her hand curls around her ankle, a double-sided pencil braided through her fingers. Her socks are loose and worn at the heel. She's ready to write, not run. Since 1974 these twins, along with a host of their non-biological sisters in the CRC, had theorized about the material specificities of black women's conditions. Their statement, which shook up feminism forever the year before this photograph was taken, coined the phrase "identity politics" and argued that racism, sexism, capitalism, homophobia, and imperialism could not be understood as separate issues. This black-and-white photograph captures the accessories of a life of activism and study: books, stacks of documents, a rotary telephone, a reading lamp, file cabinets. Further into the horizon, pinned onto the wall, two portraits reflect an endless cascade of black women's intellectual labor and political action. Up top is the journalist Ida B. Wells, and just below her is Harriet Tubman, who led the 1863 raid on the Combahee River in South Carolina that freed 750 enslaved people, and after which the CRC is named. It is easy to imagine the walls of present and future activists adorned with this very photo of the Smiths. [End Page 136] Tiana Reid Tiana Reid is a Postdoctoral Research Associate in the Department of English at Brown University and a former editor of the New Inquiry and Pinko. Copyright © 2022 Foundation for the Study of Independent Social Ideas, Inc. (FSISI).

Editorial introductions

Editorial introductions

Women Rising: In and Beyond the Arab Spring, RitaStephan and Mounira M.Charrad, eds. NYU Press, 2020. 422 pages. $99 cloth.Embodying Geopolitics: Generations of Women's Activism in Egypt, Jordan, and Lebanon, by NicolaPratt.: University of California Press, 2020. 328 pages. $85 cloth $29.95 paper.

Middle East PolicyVolume 28, Issue 3-4 p. 253-256 BOOK REVIEW Women Rising: In and Beyond the Arab Spring, Rita Stephan and Mounira M. Charrad, eds. NYU Press, 2020. 422 pages. $99 cloth. Embodying Geopolitics: Generations of Women's Activism in Egypt, Jordan, and Lebanon, by Nicola Pratt.: University of California Press, 2020. 328 pages. $85 cloth $29.95 paper. Meriem Aissa, Meriem Aissa Post-doctoral research associate, Texas Woman's UniversitySearch for more papers by this author Meriem Aissa, Meriem Aissa Post-doctoral research associate, Texas Woman's UniversitySearch for more papers by this author First published: 17 January 2022 https://doi.org/10.1111/mepo.12600Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Volume28, Issue3-4Fall-Winter 2021Pages 253-256 RelatedInformation

Honey Bees, Almonds, and Colony Mortality: An Economic Simulation of the U.S. Pollination Market

Chengcheng J. Fei, Postdoctoral Research Associate Department of Agricultural Economics, Texas A&M University, College Station; feiccheng{at}tamu.edu

Notes on Contributors

Notes on Contributors

First person – Xin Huang

ABSTRACTFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Xin Huang is first author on ‘Dissecting dynamics and differences of selective pressures in the evolution of human pigmentation’, published in BiO. Xin conducted the research described in this article while a PhD student in Li Jin, Yungang He's laboratory, Shanghai, China. He is now a Postdoctoral Research Associate in the lab of Ryan Gutenkunst at University of Arizona, Tucson, AZ, USA, investigating new methods and tools to quantify natural selection from large-scale population genomic data.