Introduction: Many adult and pediatric providers lack comfort with end-of-life (EOL) care. This is a challenge in medical education, since most training programs lack comprehensive EOL curriculums. Methods: The seminar was held at the Children’s Hospital of Pittsburgh of UPMC. Large group lectures included EOL communication skills and advance directive planning with a discussion panel on spirituality, uncertainty, and EOL care. Small group sessions included talking to children about death, advance directives, breaking bad news, and decision making. Attendees completed anonymous paired pre and post-conference surveys using Likert scales to assess comfort with EOL care domains. Surveys were analyzed using paired t-tests to calculate comfort with individual domains and a summation of all domains to determine overall comfort. Results: Surveys were completed by 63 attendees. Attendees noted significant improvement in overall comfort across all domains (mean pre score 3.36, mean post score 3.92, p <0.001). Clinical staff (nurses, nurse practitioners, and physician assistants) had the most significant improvements (3.47, 4.19, p<0.001); followed by ancillary staff (social workers, spiritual care providers and counselors) (3.76, 4.35, p <0.001); then trainees (residents, fellows, and medical students) (3.02, 3.42, p<0.001). Trainees’ ability to attend all sessions was limited due to clinical responsibility, and they did not show improvement in all areas. The conference was beneficial for participants with a palliative care focus (3.96, 4.47, p<0.001) and even more so for those without (3.18, 4.47, p<0.001). Attendees with EOL care experience had significant improvements (3.77, 4.28, p<0.001), except for knowledge of advance directives. Less experienced attendees had significant improvements across all domains (2.93, 3.53, p<0.001). Conclusions: This EOL and communication seminar was well attended and beneficial to both experienced and less experienced clinicians and staff. Attendees’ comfort levels were improved except for advance directives. This is not surprising, advance directives are rare in pediatrics and this area may require more emphasis. Finally, trainee attendance was limited; future conferences should accommodate trainee schedules as they have the potential to impact the greatest number of patients and families.