Two new species of Seinura are described and illustrated using both morphological and molecular characters. Seinura persica n. sp. is characterized by having females with a distinct cephalic region, stylet 19.5 (18-20) μm long, four incisures in the lateral fields, position of excretory pore anterior to the base of metacorpus, post-uterine sac 54.8 (50-60) μm long, and an elongate-conoid tail with a shallow depression on the dorsal side ending in a sharply pointed tip. Males have body length of 467 and 592 μm, spicules 15 and 16 μm long with a prominent triangular-shaped rostrum, developed condylus and no cucullus. Seinura hyrcania n. sp. is characterized by having an offset lip region, stylet 21.5 (18-24) μm long, three incisures in the lateral fields, excretory pore anterior to the base of the metacorpus, post-uterine sac 19 (12-25) μm long and conical tail ending in a filiform terminus. Morphological and morphometric diagnostic characters together with molecular data based on partial sequences of SSU and LSU of the ribosomal DNA gene are provided for these new species.