Background: A number of studies have investigated transgenerational effects of parental post-traumatic stress disorder (PTSD) and its repercussions for offspring. Few studies however, have looked at this issue in the African context. Methods: The present study addresses this gap, utilizing confirmatory factor analysis (CFA), to investigate symptom severity within the three Diagnostic and Statistical Manual of Mental Disorders IV (DSM-IV) PTSD symptom domains in mothers exposed to the genocide against the Tutsi in Rwanda (n=25) and offspring (n=25), and an ethnically matched control group of mothers (n=25) and offspring (n=25) who were outside of Rwanda during the genocide. All mothers were pregnant during the time of the genocide with the offspring included in the study. Missing data were excluded from the analyses. Results: We found that among the three symptom domains of PTSD, the re-experiencing symptom domain loaded most strongly onto PTSD among mothers directly exposed to the genocide (Beta = 0.95). In offspring of exposed mothers, however, the three symptom domains of PTSD yielded almost equal loading values (Beta range = 0.84-0.86). Conversely, among non-exposed mothers and their offspring, the hyperarousal symptom domain of PTSD loaded most strongly onto PTSD (Beta = 1.00, Beta = 0.94, respectively). As a secondary analysis, we also explored the relation between DNA methylation in the glucocorticoid receptor (NR3C1) locus, an important stress modulating gene, and individual PTSD symptom domains, finding a strong association between DNA methylation and re-experiencing among genocide-exposed mothers that exceeded any other observed associations by approximately two-fold. Conclusions: This is the first report, to our knowledge, of a symptom-based analysis of transgenerational transmission of PTSD in Africa. These findings can be leveraged to inform further mechanistic and treatment research for PTSD.