This paper developed an object-oriented software to facilitate parameter calibration of the quasi-zero stiffness (QZS) vibration isolator for engineering applications. Considering its advantages of convenient programming and high noise resistance, we employ the harmonic balance method to construct the identification core algorithm. The developed software consists of data pre-processing, parameter identification, and post-processing modules. The data pre-processing module imports and classifies all the required data. Then, the parameter identification module conducts preliminary nonlinearity analysis so that nonlinear structural parameter identification can be successfully carried out. After parameter identification, the data post-processing module displays and stores the identified results. The performance of the software is tested by a numerical example of a two-degree-of-freedom QZS vibration isolator, proving the software can give accurate identification results within a few user-friendly graphical interfaces. Based on this software, we hope that the application and promotion of nonlinear system parameter identification become more convenient.