During the interventional operation of patients with gastric disease under nano gold biosensor, the nursing staff should have professional nursing knowledge. High quality nursing is a kind of nursing intervention based on holistic nursing and patient-centered. It can make corresponding nursing measures according to the actual condition of patients, so that patients can feel high-quality nursing services. In this study, high-quality nursing measures were taken for patients with gastric diseases in the interventional treatment of nano gold biosensor. Before the operation, relevant psychological nursing measures were given to patients, and related knowledge of disease and operation were explained. The skillful movements in operation and the cordial sympathy after operation can appease the patients' bad psychological state to a certain extent, so as to effectively reduce the patients' anxiety, depression and other psychological emotions. High quality nursing intervention for patients with gastric diseases under the intervention of nano gold biosensor can effectively improve the degree of anxiety and depression of patients, the recovery of bowel sounds and the time of getting out of bed activities, improve the treatment compliance, and promote the early recovery of patients' health.