Post-hoc Tukey HSD Research Articles

Leveraging Ensemble Models and Follow-up Data for Accurate Prediction of mRS Scores from Radiomic Features of DSC-PWI Images.

Predicting long-term clinical outcomes based on the early DSC PWI MRI scan is valuable for prognostication, resource management, clinical trials, and patient expectations. Current methods require subjective decisions about which imaging features to assess and may require time-consuming postprocessing. This study's goal was to predict multilabel 90-day modified Rankin Scale (mRS) score in acute ischemic stroke patients by combining ensemble models and different configurations of radiomic features generated from Dynamic susceptibility contrast perfusion-weighted imaging. In Follow-up studies, a total of 70 acute ischemic stroke (AIS) patients underwent magnetic resonance imaging within 24hours poststroke and had a follow-up scan. In the single study, 150 DSC PWI Image scans for AIS patients. The DRF are extracted from DSC-PWI Scans. Then Lasso algorithm is applied for feature selection, then new features are generated from initial and follow-up scans. Then we applied different ensemble models to classify between three classes normal outcome (0, 1 mRS score), moderate outcome (2,3,4 mRS score), and severe outcome (5,6 mRS score). ANOVA and post-hoc Tukey HSD tests confirmed significant differences in model style performance across various studies and classification techniques. Stacking models consistently on average outperformed others, achieving an Accuracy of 0.68 ± 0.15, Precision of 0.68 ± 0.17, Recall of 0.65 ± 0.14, and F1 score of 0.63 ± 0.15 in the follow-up time study. Techniques like Bo_Smote showed significantly higher recall and F1 scores, highlighting their robustness and effectiveness in handling imbalanced data. Ensemble models, particularly Bagging and Stacking, demonstrated superior performance, achieving nearly 0.93 in Accuracy, 0.95 in Precision, 0.94 in Recall, and 0.94 in F1 metrics in follow-up conditions, significantly outperforming single models. Ensemble models based on radiomics generated from combining Initial and follow-up scans can be used to predict multilabel 90-day stroke outcomes with reduced subjectivity and user burden.

Colloidal platinum nanoparticles enhance resin-dentin bonding durability

ObjectivesThis study aims to investigate the effect of colloidal platinum nanoparticles (CPN) on the durability of resin-dentin bonding performance with contemporary adhesives. MethodsSixty non-carious human maxillary premolars were subjected to microtensile bond strength (µTBS) testing and divided into two main groups: CPN-treated and untreated. Within each group, specimens were randomly allocated to Clearfil Megabond 2 (MB2), Scotchbond Universal Plus Adhesive with self-etch mode (SE-SUP), and etch-and-rinse mode (ER-SUP) subgroups (n = 10/group). CPN was applied to dentin in the MB2 and SE-SUP groups for 20 s, followed by rinsing before adhesive application. In the ER-SUP group, CPN was applied after etch-and-rinse. The µTBS was tested after 24 h, 6 months, and 1 year, and the fracture modes were observed using SEM. The µTBS data were analyzed using a two-way ANOVA and post-hoc Tukey HSD test (α = 0.05). An additional twelve premolars underwent TEM/STEM/EDX for ultra-morphological observations. ResultsThe application of CPN significantly prevented a decline in the µTBS of both the MB2 and SE-SUP groups. No significant decrease was observed in the ER-SUP group, either with aging or CPN application. Ultra-morphological images revealed platinum nanoparticles attaching to the collagen fibrils of the hybrid layer regardless of aging. It was highlighted that the nanoparticles attached to the banded collagen in the aging groups were observed. SignificanceCPN exhibits the potential in enhancing the longevity of resin-dentin bonding in SE mode.

Photoactivated riboflavin-doped hydroxy apatite nanospheres infiltered in orthodontic adhesives.

To assess micro-tensile bond strength (μTBS), degree of conversion (DC), microleakage (ML) antibacterial efficacy, and adhesive remnant index (ARI) of orthodontic brackets to enamel with different concentrations of photoactivated riboflavin-doped hydroxyapatite (HA) nanospheres (NS) (0%,1%,5% and 10%) and 0.5 wt% RF alone in orthodontic adhesive. Samples were included on the predefined inclusion criteria and positioned up to the cementoenamel junction (CEJ). Hydroxy apatite nanospheres (HANS) commercially bought were doped with RF. Surface characterization of HANS and RF-doped HANS were assessed along with EDX analysis. Samples were grouped based on experimental orthodontic adhesive modification. Group 1: Transbond XT no modification, Group 2: experimental Transbond XT 0.5 wt% RF, Group 3: experimental Transbond XT 0.5 wt% RF-doped 1% HANS, Group 4: experimental Transbond XT 0.5 wt % RF-doped 5% HANS and Group 5: Experimental Transbond XT 0.5 wt% RF-doped 10% HANS. Brackets were placed based on different adhesive modifications and samples underwent thermocycling. Samples were evaluated for μTBS, DC, and ML. The type of failure was assessed using ARI. Adhesive modified and un-modified in four different concentrations (0%, 1%, 5%, and 10%) and 0.5 wt% RF only were used to test efficacy against Streptococcus mutans (S.mutans). The survival rate of S.mutans and ML was determined using the Kruskal-Wallis Test. For the analysis of μTBS, ANOVA was employed, followed by a post-hoc Tukey HSD multiple comparisons test. The highest μTBS and lowest ML were observed in Group 2 experimental Transbond XT 0.5 wt% RF only. The lowest μTBS, highest ML, and lowest DC was seen in Group 5 experimental Transbond XT 0.5 wt% RF-doped 10% HANS. Samples in Group 1 in which Transbond XT was used as adhesive demonstrated significantly the highest microbial count of S.mutans and DC. Photoactivated RF-doped HANS in 1% and 0.5 wt% Riboflavin alone in orthodontic adhesive for metallic bracket bonding improved micro tensile bond strength, ML, DC, and antibacterial scores. RESEARCH HIGHLIGHTS: The highest μTBS and lowest ML were observed in Group 2 experimental Transbond XT 0.5 wt% RF only. The lowest μTBS, highest ML, and lowest DC was seen in Group 5 experimental Transbond XT 0.5 wt% RF-doped 10% HA-NS. Samples in Group 1 in which Transbond XT was used as adhesive demonstrated significantly the highest microbial count of S.mutans and DC.

A Virtual Multi-institution Pediatric Radiology Peer Teaching Conference Improves Knowledge of Educators

Rationale and ObjectivesMedical education led by peers and near-peers has been shown to benefit both teachers and learners and can be successfully incorporated into radiology education. The authors created a virtual, multi-institution pediatric radiology conference employing peer and near-peer teaching with the goals of improving radiology knowledge and enhancing the educational experience of radiology trainees. Materials and MethodsTwo radiology residency programs implemented a common pediatric radiology curriculum and joint quarterly virtual peer teaching conference. Conferences featured short teaching sessions led by six to ten radiology trainees and were facilitated by attending pediatric radiologists. Knowledge assessments (KA) consisting of multiple-choice questions inspired by conference learning objectives were sent to peer educators before the conference (pre-conference), directly after the conference (immediate post-conference), and three months after the conference (delayed post-conference). Surveys were distributed to peer educators immediately after conferences to assess conference reception and solicit feedback. Quantitative data was analyzed using ANOVA, Kruskal-Wallis test, and post-hoc Tukey HSD test. ResultsFour conferences featured 33 peer educators consisting primarily of first-year (60.6 %), second-year (18.2 %), and third-year (15.2 %) radiology residents. Compared to pre-conference scores, immediate post-conference scores were significantly increased (HSD 13, p = 0.02) and delayed post-conference scores were increased without statistical significance (HSD 5.8, p = 0.29). Almost all survey respondents perceived the conferences as helpful, well-organized, and effective in teaching pediatric radiology. A majority of participants expressed interest in participating in future peer teaching radiology conferences. ConclusionA virtual pediatric radiology peer and near-peer teaching conference held between two radiology residencies improved short-term radiology knowledge of educators and was highly received.

Evaluating the Remineralizing Effects of Calcium Sucrose Phosphate and Casein Phosphopeptide-Amorphous Calcium Phosphate Toothpastes on Artificial Carious Lesions Using Micro-computed Tomography: An In Vitro Investigation.

Background White spot lesions (WSLs) are common early indicators of enamel demineralization, particularly in pediatric orthodontic patients. Effective remineralization of these lesions is crucial for preventing further dental decay. This study aimed to evaluate the three-dimensional remineralization efficacy of two commercial toothpastes, calcium sucrose phosphate (CaSP) and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), using micro-computed tomography (micro-CT). Objectives To compare the remineralization efficacy of CaSP and CPP-ACP on artificially created WSLs in human premolar enamel using micro-CT assessment. Materials and methods Freshly extracted, caries-free human premolars were used for the purpose of the study. Teeth with any defects, including caries, non-carious lesions, fractures, or hypocalcifications, were excluded. Teeth were stored in a saline solution with 0.1% thymol at +4°C until experimentation. Enamel slabs (N = 18) of 3 x 3 x 1.5 mm were prepared from the buccal surfaces of the premolars and polished to a mirror-like finish. The slabs were divided into three groups (n=6 each): control, CaSP, and CPP-ACP. Specimens were demineralized in a demineralization solution for 72 hours and then treated with the respective toothpastes or remineralizing solutions for 13 days. Micro-CT scanning was performed to assess changes in enamel volume and mineral density. Statistical analysis included Shapiro-Wilk tests, paired t-tests, and one-way ANOVA with post-hoc Tukey's HSD tests. Results Enamel volume changes were significant between groups (p<0.01), with the CaSP group showing the largest remineralization effect. Enamel mineral density changes were also significant (p=0.004), with the CPP-ACP group showing the greatest improvement in mineral density. Conclusions CaSPand CPP-ACPare both effective in remineralizing artificial enamel lesions. While CaSP shows comparable efficacy to CPP-ACP, further research is needed to confirm these findings in clinical settings. CaSP pastecan be considered a viable, cost-effective alternative for enamel remineralization.

Effect of the Application Procedure on The Bond Strength of Universal Adhesive in Restoration Repair

Objective: This study aimed to evaluate the effect of reduced application time on the bond strength of a universal adhesive used for the repair of hybrid ceramic and composite resin restorations. Methods: In the study, a total of 48 rectangular prisms of sizes 7×6×2 mm hybrid ceramic (Vita Enamic, Zahnfabrik, Germany) and composite resin (Solare X, GC, Japan) specimens were prepared. All samples were aged with 5000 thermal cycles. Then, the upper surfaces of the samples were roughened with a diamond fissure bur (10–36µ, 852 FG Meisinger; Hager &amp;amp; Meisinger GmbH, Germany) with 5 strokes under water cooling. The samples were divided into 2 subgroups according to the different application times of the adhesive system (G-Premio Bond, GC, Japan) (n=12). Then, composite resin (Solare X, GC) was placed using plastic cylinder molds with 3 mm inner diameter and height and cured with light for 20 seconds. All samples were thermal aged and then a shear bond strength test was conducted. The data were analyzed with Two-way ANOVA and post-hoc Tukey HSD tests (p˂0,05). Results: Two-way ANOVA revealed that the repair bond strength of the 10 seconds of waiting was significantly higher than the quick application for both hybrid ceramic and composite resin (p˂0,05). It was determined that there was no significant difference between hybrid ceramic and composite resin in the both quick application group and 10 seconds waiting group (p˃0,05). Conclusion: Quick application procedure showed lower shear bond strength than 10 seconds waiting group for the repair of both composite resin and hybrid ceramic (p&amp;lt;0,05). Keywords: Quick application, Application time, Universal adhesive, restoration repair..

Effectiveness of Gamified Cooperation and Competition in Blended Learning Environment for EFL Business Writing in TVET

This research investigated the effectiveness of gamified blended learning environments in fostering EFL writing skills among technical and vocational education and training (TVET) trainees in Saudi Arabia. It compared three distinct learning strategies achieved through gamification design in a blended delivery mode: gamified cooperation, gamified competition, and gamified cooperation-competition. Each strategy leveraged a specific category of gamification features that evoke different goal structure: cooperation, competition, or a combination of both. A quasi-experimental design with a pre-test and post-test was employed. Forty-five trainees were purposively selected and randomly assigned to three groups, each receiving instruction using a different gamified blended learning program for teaching business writing. The findings revealed significant improvement in English as a Foreign Language business writing skills for all three groups. However, a one-way ANOVA and post-hoc Tukey HSD test indicated that the gamified cooperation-competition strategy yielded the greatest improvement compared to the gamified cooperation and gamified competition strategies alone. This suggests that a combination of cooperative and competitive elements within a gamified blended learning environment might be the most effective approach for enhancing English as a Foreign Language business writing skills among Technical and Vocational Training and Education learners.

Maternal and Neonatal Outcomes with One Abnormal Value on 100g Three Hour Glucose Tolerance Test in Pregnancy

Purpose: The primary objective of this study was to compare pregnancy outcomes for patients with no elevated glucose values, one elevated glucose value, and 2 or more elevated glucose values on the 3-hour glucose tolerance test (GTT). Methods: This was a retrospective chart review of obstetric patients seen by residents and attending physicians at a single institution from 2015 to 2020 who were required to complete a 3-hour glucose tolerance test (GTT). Data extracted from the maternal chart included demographic information, BMI, route of delivery, presence of preeclampsia, chorioamnionitis, shoulder dystocia and preterm labor. Data extracted from the neonatal chart included blood glucose level after birth, birth weight, Apgar scores, and if NICU admission was required. Continuous data were analyzed using one-way ANOVA to compare the 3 groups, followed by post-hoc Tukey HSD to compare the 1 abnormal value group to the control group. Dichotomous data were analyzed using Chi square tests. Logistic regression was performed using shoulder dystocia as the outcome. Results: Patients with two or more elevated GTT values had higher age and BMI and were more likely to be prescribed diabetic medications compared to those with normal or one elevated value. They were also more likely to deliver at an earlier gestational age. Patients with one elevated GTT value experienced a statistically significant higher chance of shoulder dystocia compared to patients with all normal GTT results (OR 2.34; 95% CI 1.05, 5.20). Patients with two or more elevated values were more likely to have preeclampsia and a neonate requiring NICU admission. There were no differences in cesarean delivery rate, hemorrhage or chorioamnionitis between the groups. Conclusions: Patients with one elevated GTT value are a special subset of patients who do not meet criteria for gestational diabetes, yet do not have normal glucose testing results. We hypothesize that this group is at higher risk of shoulder dystocia because these patients may have hyperglycemia but often do not receive the same type of monitoring, dietary education, medication initiation and induction timing as patients who meet criteria for gestational diabetes.

Promoting Ecosystem Conservation and Food Security through Sustainable Harvesting of Edible Caterpillars in Kanchibiya District, Zambia

Insects play a vital role in enhancing food security and improving the livelihoods of rural communities. This study focused on the identification of caterpillar harvesting practices in Zambia, particularly in Chief Kopa’s village, Kanchibiya District, Muchinga Province, where the caterpillars of Gynisa maja and Gonimbrasia belina play a crucial role in food security. A mixed-methods approach was employed, utilizing face-to-face interviews and self-administered questionnaires to capture both qualitative and quantitative insights. Quantitative data, including caterpillar harvesting amounts and correlations with tree destruction, underwent statistical analyses, including ANOVA and post-hoc Tukey's HSD tests. The Chi-square test explored gender distribution across research sites. Qualitative data from open-ended questions during interviews underwent thematic analysis. The study sampled 100 respondents from six areas, and the results revealed significant differences in caterpillar harvesting amounts among villages. Post-hoc tests identified specific pairwise differences. The findings indicated that traditional harvesting methods persisted, with limited adherence to rules. A significant positive correlation was observed between caterpillar harvesting practices and evolving trends such as increased harvesting intensity, changes in harvesting methods, and environmental impact and tree destruction (p=0.001, r=0.917). The study contributed valuable insights into past caterpillar harvesting practices, proposing conservation strategies for enhanced food security and sustainability. The study proposes a multifaceted approach to balance ecosystem conservation with food security in Kanhaiya District, emphasizing the importance of sustainable caterpillar harvesting. Recommendations include developing community-based sustainable harvesting guidelines, such as rotational zones and harvest limits, alongside education programs to raise ecological awareness. It advocates for diversifying livelihoods and food sources to lessen dependence on caterpillars, ensuring gender-inclusive participation in these initiatives. Monitoring and further research are essential to adapt strategies effectively. These measures aim to preserve the ecosystem, ensuring the caterpillar's role in food security and offering socioeconomic benefits by fostering community resilience and reducing food insecurity vulnerabilities

Characterization of patient population receiving bioactive glass bone graft substitute as intraoperative treatment for orthopaedic trauma fractures

BackgroundBioactive glass synthetic bone grafts are used to treat osseous defects in orthopaedic surgery. Characterization of the clinical scenarios associated with bioactive glass use in the context of orthopaedic trauma, are not well established. This study aims to characterize population demographics, operative variables, as well as postoperative variables, for patients who required bone grafting for treatment of traumatic orthopaedic injuries and received a bioactive glass bone substitute intraoperatively. MethodsThe electronic medical record at a large Level I trauma center was queried for fracture patients between January 1st, 2019, and April 30th, 2022. Our retrospective cohort included fracture patients who received Fibergraft Matrix or Fibergraft Putty intraoperatively, and their respective control groups. This study ascertained patient demographic variables, operative variables, and postoperative variables. Differences in categorical variables were tested with Fischer's Exact Tests, while differences in continuous variables were tested with ANOVA. Statistical significance was determined as P < 0.05. If the overall Group model was significant for a given variable, post-hoc Fischer's Exact or Tukey HSD tests were used to assess pairwise significance between individual Group pairs. ResultsA total of four categories across our analysis of demographic, operative, and postoperative variables displayed significant differences amongst subject Groups (P ≤ 0.03). Individual groups were compared such that significant differences between subject groups could be appreciated for a specific variable. FM subjects had greater length of surgery, billable costs, and vitamin D supplementation at the time of surgery compared to FM controls. Similarly, FP subjects had greater length of surgery, billable cost, and implants used intraoperatively compared to FP controls. ConclusionThis analysis revealed Fibergraft patients to have greater length of surgery and billable cost, with respect to their matched controls. These data suggest that Fibergraft patients had more severe orthopaedic fractures compared to matched controls.

Potential Test of Jojoba Oil-Based Sunscreen in Lotion Preparations

This study aimed to determine the formula of anti-aging cream using lemongrass leaf extract. The treatment by giving respondents lemongrass leaf extract and using moisture, evenness, pore, spot, and wrinkle as parameters. Human skin provides protection on the surface of the body against the influence of the surrounding environment. Skin can experience aging, especially in areas that are often exposed to direct sunlight such as the face, neck, upper arms, and hands. Aging is a process experienced by humans in their 30s whose functions in the human body are decreasing, for example, thinning skin, wrinkles, rough skin, and dark spots on the face. Treatment to prevent aging of the skin by using an antioxidant product such as flavonoids because it has the function of preventing or neutralizing free radicals. In Indonesia, several species of plants can be found that function as food and cosmetic ingredients. The lemongrass leaf extract which contains antiaging are Alkaloids, Flavonoids, and Tannins. The data were analysed by using the Kolmogorov-Smirnov method to determine homogeneity and normality. Then, if the data is normal, it is continued to be analysed using the One-Way ANOVA method to determine the average difference between groups. There is a difference between treatments, and it is continued with the Post-hoc Tukey HSD test. The higher the concentration of extract used, the greater the anti-aging productivity formed. It is concluded, the best anti-aging effect is a concentration of 9% with increase in moisture, evenness, pore reduction, reducing spots and wrinkles.

Antiaging Cream Formula From Lemongrass Leaf Extract (Chymbopogon Citratus)

This study aimed to determine the formula of anti-aging cream using lemongrass leaf extract. The treatment by giving respondents lemongrass leaf extract and using moisture, evenness, pore, spot, and wrinkle as parameters. Human skin provides protection on the surface of the body against the influence of the surrounding environment. Skin can experience aging, especially in areas that are often exposed to direct sunlight such as the face, neck, upper arms, and hands. Aging is a process experienced by humans in their 30s whose functions in the human body are decreasing, for example, thinning skin, wrinkles, rough skin, and dark spots on the face. Treatment to prevent aging of the skin by using an antioxidant product such as flavonoids because it has the function of preventing or neutralizing free radicals. In Indonesia, several species of plants can be found that function as food and cosmetic ingredients. The lemongrass leaf extract which contains antiaging are Alkaloids, Flavonoids, and Tannins. The data were analysed by using the Kolmogorov-Smirnov method to determine homogeneity and normality. Then, if the data is normal, it is continued to be analysed using the One-Way ANOVA method to determine the average difference between groups. There is a difference between treatments, and it is continued with the Post-hoc Tukey HSD test. The higher the concentration of extract used, the greater the anti-aging productivity formed. It is concluded, the best anti-aging effect is a concentration of 9% with increase in moisture, evenness, pore reduction, reducing spots and wrinkles.

Evaluation of commercial doses of a feed additive and silymarin on broiler performance with and without CCl4-induced liver damage

Improving productive performance is a daily challenge in the poultry industry. Developing cost-effective additives and strategies that improve performance in antibiotic-free poultry production is critical to maintaining productivity and efficiency. This study evaluates the influence of a commercially available phytogenic feed additive (CA-PFA, that comprises silymarin, betaine and curcumin extracts as main ingredients) and silymarin on commercial broilers' productive performance and liver function with and without carbon tetrachloride (CCl4)-induced liver damage. The experiment was conducted in a completely randomized design, with six treatments, eight replicates, and eight birds per replicate in 18 one-day-old male broilers (Cobb Vantress 500) each; under a 3 × 2 factorial arrangement (3 diets x 2 levels of CCl4, 0 and 1 mL/kg body weight orally). The experimental treatments included 3 diets, commercially recommended doses of CA-PFA (500 mg/kg of feed; this dose provides 70 mg/kg of silymarin, besides the other active ingredients included in the formulation), silymarin (250 mg/kg of feed, containing 28% of active ingredient; this dose provides 70 mg/kg of silymarin as active ingredient) and an additive-free basal diet as a control. A standard commercial silymarin was used as a reference due to its well-known and extensively studied hepatoprotective properties that can mitigate the negative effects of CCl4 in the liver. The data were analyzed as a 2-way ANOVA, and the means showing significant (P ≤ 0.05) differences were then compared using the Post-Hoc Tukey HSD test. No interaction was detected between factors. Exposure to CCl4 had a noticeable detrimental effect on alertness, productive performance, and liver function of broilers without a significant increase in mortality. Including CA-PFA in the diet improved productive performance compared to the basal diet from day 21 to the end of the trial, on day 42. While no influence in feed intake was detected for any treatment, CA-PFA improved body weight gain (BWG) and feed conversion ratio (FCR) significantly (P < 0.05) from day 21 to the end of the trial in healthy and CCl4-exposed birds. The results show that CA-PFA supplementation improves performance parameters in broilers with and without CCl4-induced liver damage, when compared to a basal diet and the addition of a standard commercial silymarin product.

Comparative Analysis of Physiochemical Properties and In Vitro Antioxidant Potency Among Morphotypes of Centella asiatica Grown in Sri Lanka

Centella asiatica commonly referred as Gotukola, is recognized for its extensive application in traditional medicine owing to its remarkable health benefits. This study investigated the differences in pH, ash content, moisture content, and in vitro antioxidant potential among three prevalent vine- type morphotypes of C. asiatica: Wel Gotukola, Meerigima Gotukola, and Yodha Gotukola, indigenous to Sri Lanka. C. asiatica plants of the same age (100 days) at their first harvestable matured stage, were randomly collected from domestic gardens in Gampaha District, Sri Lanka. Three replicates of each morphotype were sampled. Moisture and ash content were analysed using the standard AOAC method, and pH was measured using the potentiometric method. DPPH (2,2- Diphenyl-1-picrylhydrazyl) radical scavenging capacity assay was used to determine the antioxidant activity. To thoroughly compare antioxidant potential among morphotypes, the ANOVA test was utilized followed by post-hoc Tukey HSD tests. Following results were obtained for the average moisture content of Wel Gotukola (83.41%±1.16%), Yodha Gotukola (74.33%±2.23%), and Meerigama Gotukola (79.46%±1.89%), alongside average ash content of Wel Gotukola (11.60%±1.02%), Yodha Gotukola (13.12%±2.57%), and Meerigama Gotukola (12.17%±2.01%). Additionally, an average pH of Wel Gotukola (6.00±0.21), Yodha Gotukola (5.75±0.25), and Meerigama Gotukola (5.98±0.14) was observed. The antioxidant activity of C. asiatica extracts exhibited concentration dependent increases in radical scavenging capacity. Notably, the DPPH radical scavenging potency, denoted by the minimum IC50 value, exhibited significant variations across different Gotukola varieties (p&lt;0.05): Wel Gotukola (6.4 ± 0.54 µg/mL), Yodha Gotukola (8.56±0.35 µg/mL), and Meerigama Gotukola (7.0±0.75 µg/mL). Post-hoc Tukey tests showed antioxidant activity was significantly higher in Wel Gotukola compared to that of Meerigama and Yodha Gotukola morphotypes (p&lt;0.05). Furthermore, Wel Gotukola demonstrated the highest average pH, moisture content, and the lowest mineral content. Conversely, Yodha Gotukola exhibited the lowest pH, moisture content, and the highest ash content. These findings underscore the potential of chemical parameters in elucidating the distinctive attributes of the three Gotukola varieties. Specifically, the significantly higher antioxidant potential observed in Wel Gotukola suggests its potential as an optimal candidate for herbal product development. This study imparts valuable insights into optimizing the therapeutic efficacy of formulations based on the specific C. asiatica employed in such endeavours.&#x0D; &#x0D; Keywords: Antioxidant activity, Centella asiatica, Morphotypes, Proximate analysis

Wettability of Different Zirconia Implant Surfaces after Glycine and Erythritol Treatment.

This in-vitro study aims to investigate whether the wettability of two zirconia implant disk surfaces is affected by glycine and erythritol air-polishing, using saline, bovine serum albumin, bovine thrombin, and bovine artificial blood as the testing mediums. Zirconia disks were provided by two implant companies: SDS. (Swiss Dental Solutions, Plymouth, MA, USA) (n=6) and Patent. (Zircon Medical AG, Altendorf, Switzerland) (n=6). Surface parameters (Sa, Sz, Sdr, Ssk, Sku, and Spd) were measured to determine their initial surface roughness. Air-polishing was completed using glycine or erythritol powders using the AIRFLOW. PERIO device (Manufactured by Electro Medical Systems (EMS)). The wettability of the disk surfaces was evaluated by the contact angle analysis applied on treated and untreated disks using four wetting solutions, saline, bovine serum albumin (BSA), bovine thrombin, and bovine artificial blood. In total, 720 contact angle measurements were completed with n=30 per group. Statistical analysis was performed using the one-way analysis of variance and post-hoc Tukey HSD test. The results indicate SDS. has a greater wettability than Patent. before and after air-polishing. The wettability of surfaces treated with glycine was hydrophobic in some cases. Erythritol increased the surface wettability of zirconia disks compared to glycine. BSA and bovine artificial blood had lower contact angles than saline and bovine thrombin for both untreated disks. The initial wettability of zirconia disks differs between manufacturers, and air-polishing with erythritol powder increases the wettability of zirconia implant disks for saline, bovine serum, bovine thrombin, and bovine artificial blood.

Early growth of porang (Amorphophallus oncophyllus) with planting distance adjustment under oil palm plantation

Porang (Amorphophallus oncophyllus) thrive under shaded conditions and has been planted under plantations. As oil palm plantations area in Aceh is growing; the effect of shading under oil palm plantation has not been previously reported. The material used is bulbil from the porang plant. Meanwhile, the oil palm stands used were 8-10 years old with the canopy covering >80% of the land. The experimental design used is a Completely Randomized Block Design with the treatment of spacing settings of 40 x 40 cm; 75 x 75 cm; 80 x 80 cm; 90 x 90 cm and 100 x 100 cm. Experiments were conducted in four replicates. Data were analysed using One Way Anova, followed by posthoc Tukey HSD test. Results shows that the spacing had a significant effect on its growth, especially the age of shoot emergence, plant height, stem diameter and number of leaves. The age of budding which allows early shoots to appear is at a spacing of 40 x 40 cm, as well as the plant height parameters at observations 4, 5, 6 and 7 WAP. Meanwhile, at the age of 8 and 9 WAP, the best plant height growth was at 80 x 80 cm spacing.

Opening Pandora's box: diagnostics characters for the confuse taxonomy of the Brazilian Cardiomya (Bivalvia: Cuspidariidae)

Abstract The unsolved systematics of the genus Cardiomya has led to a sequence of astonishing identification mistakes. This scenario is a result of the rarity of specimens and, more importantly, the lack of knowledge about which characters are relevant to the genus taxonomy. In this study, we developed a method based on standard linear discriminant analysis to identify the smallest number of morphological characters that efficiently distinguish individuals at the species level of Brazilian Cardiomya. Starting from 29 morphometric measurements obtained from photographed Cardiomya shells, we were able to identify only five characters: the dorsal inflection of the rostrum, the distance from the posterior most rib end to the umbonal posterior margin and the distance from the central point of the valve to the anterior margin at 45°, 15° and −30° angles. Surprisingly, all these characters are related to the shell outline and not the ornamentation, which is a remarkable character in Cardiomya. We performed a one-way ANOVA with post-hoc Tukey HSD test specifically using the total number of ribs to verify its discriminant power in species identification. Our analysis demonstrated that the number of ribs does not show a significant difference between the analysed species.

ВПЛИВ ТЕМПЕРАТУРИ ЛИЧИНКОВОЇ СТАДІЇ РОЗВИТКУ НА ЕКСПРЕСІЮ ГЕНІВ HSP70, INR, SIRT1, MTOR ТА FOXO У САМЦІВ І САМОК DROSOPHILA MELANOGASTER

Background. Despite the progress in understanding the phenomenon of aging, the key factors that influence this process remain poorly understood. Aging is a genetically programmed set of events, leading to structural and functional changes that reduce the life expectancy of an organism. The relevance of the study is to expand the understanding of the impact of environmental factors, in particular temperature, on the early stages of development on the life expectancy of imago, using Drosophila melanogaster as a model. The aim was to determine and analyze the expression level of genes associated with lifespan in D. melanogaster – Hsp70, InR, Sirt1, mTor and foxo – in flies reared at different temperatures of the larval stage of development. Methods. The larvae were kept at different temperatures, after which the gene expression level was determined by RT-qPCR in adult flies. The relative expression level was calculated by using the 2-ΔΔCt method. The data were analyzed using ANOVA-test followed by a pairwise multiple comparison post-hoc Tukey HSD test. Differences were considered significant at p˂0.05. Results. The temperature of the larval stage of development did not significantly affect the gene expression of male adults. Аt the same time in females imago a significant increase in the expression of Hsp70, InR, Sirt1 and mTor genes was observed in individuals with larval development took at 20°C and 30°C, compared to the control at 25°C. Conclusions. The increased expression levels of the genes chosen for analysis under the critical temperature conditions indicates the induction of a generalized stress response that did not correlate with an increased life expectancy. The finding of sex differences in gene expression patterns requires further investigation to uncover the molecular mechanisms underlying it.

Comparative Evaluation of The Microhardness of Artificial Teeth of Various Composition

Statement of the problem: To help physicians choose the right materials for treatment planning, it is necessary to investigate to the mechanical and physical characteristics of denture teeth made with new technology. Objective: This study aims to investigate the comparative microhardness of artificial teeth with different composition. Materials &amp; Methods: A total of 96 specimens (n=32) were prepared using three different types of artificial teeth (conventional polymethylmethacrylate, , and nanohybrid composite) (Group CA - Conventional PMMA, Group IS - Isosite Group DCL - double cross-linked acrylic). Surface hardness values were determined using a microhardness tester (Shimadzu HMV Corporation, Tokyo, Japan) with a load of 500 g and a dwell time of 15 seconds. Three indentations were obtained for each sample and the Vickers Hardness Number (VHN) was calculated and averaged. One-way analysis of variance (ANOVA) and post-hoc Tukey HSD tests were used for statistical analysis. The statistical significance level of the data was taken as α=0.05. Results: The highest microhardness values were observed in Group IS (53.96 ± 8.5 VHN) and the lowest in Group CA (53.55±9.9 VHN). However, no statistically significant difference was found between the groups (P&gt;0.05). Conclusions: For the durability and function of prosthetic rehabilitations performed by clinicians, the choice of artificial teeth should not be ignored, based on cost and accessibility criteria.

Comparison of The Effectiveness of Bandotan Leaves (Ageratum conyzoides Linnaeus) and Sintrong Leaves (Crassocephalum crepidioides) Extracts on The Growth of The Bacteri Staphylococcus aureus ATCC 6538

Staphylococcus aureus is a leading cause of infections and infant mortality in Indonesia, especially pneumonia. According to data from the Indonesian Ministry of Health in 2022, pneumonia accounted for 14.4% of cases in infants and 9.4% in toddlers, with 1,475 cases reported in Cirebon city. Allergic reactions from antibiotics have become a serious issue in treatment, prompting the need for natural alternatives such as bandotan leaf (Ageratum conyzoides Linnaeus) and sintrong leaf (Crassocephalum crepidioides). The compounds alkaloids, flavonoids, saponins, and tannins present in these leaves are believed to effectively inhibit bacterial growth. The study aims to compare the effectiveness of bandotan leaf extract (Ageratum conyzoides Linnaeus) and sintrong leaf extract (Crassocephalum crepidioides) on the growth of Staphylococcus aureus bacteria. Experimental research with posttest-only control group design from April to July 2023. The study used 8 groups consisting of bandotan leaf extract (Ageratum conyzoides Linnaeus) and sintrong leaf extract (Crassocephalum crepidioides) at concentrations 50%, 75%, and 100%, as well as chloramphenicol (positive control) and DMSO 10% (negative control). Data were analyzed using One-way ANOVA, Post-hoc Tukey HSD, and Independent T-test. There is a comparison of the effectiveness of bandotan leaf extract (Ageratum conyzoides Linnaeus) and sintrong leaf extract (Crassocephalum crepidioides) against the growth of Staphylococcus aureus bacteria. Sintrong leaf extract (Crassocephalum crepidioides) exhibits an average inhibitory zone effectiveness of ± 10.44083 mm (P-value 0.000). The Crassocephalum crepidioides extract group was the most effective in inhibiting the growth of Staphylococcus aureus bacteria.