Post-hoc Comparisons Research Articles

Evaluation of Mechanical Behavior of CAD/CAM Polymers for Long-term Interim Restoration Following Artificial Aging.

Mechanical properties are crucial parameters for the clinical performance of longterm CAD/CAM interim restorations. This study aimed to evaluate and compare fracture toughness (FT), flexural strength (FS), flexural modulus (FM), microhardness (H), and the brittle index (BI) of three different long-term interim restorative materials after simulated ageing. CAD-Temp; VCT, Everest C-Temp; CT, and PEEK; PK were the materials used. Specimens were divided into subgroup A (control group) with no thermocycling, and subgroup B subjected to 5000 thermocycles between 5 and 55 °C for 30 seconds. FS, FM, FT, H, microstructure, and the brittle index were evaluated. The data was examined using two-way ANOVA and Tukey's test for post-hoc comparisons. In the VCT group, FS, FT, and H were significantly reduced (p ⟨0.05) after thermocycling. In the PK group, no statistically significant differences (p ⟩0.05) were detected between the aged and non-aged groups. As for the CT group, the tested properties were significantly reduced (p ⟨0.05) after simulated aging condition. The PK group recorded appropriate mechanical behavior both before and after simulating aging, in addition to its better machinability than other materials. The CT group had better mechanical properties before and after ageing, despite the significant reduction in mechanical properties after aging.

Impact of New Living Environment on Reactive Stress Tolerance in Preadolescent Sports Trainees of Jharkhand

Study purpose. The purpose of the study was to examine the effect of background experience related to family demography on the selected parameters of reactive stress tolerance in athletes who strictly lived in a training academy for a minimum of two years. Materials and methods. A total of 200 male sports cadets were chosen by the Jharkhand State Sports Promotion Society in Ranchi, with chronological age ranging from 11 to 14 years and 4 years of training. The demographic information (the size of the family, the type of family, the order of birth, the family monthly income, and the location of the home) were considered as independent variables. Stress tolerance parameters (PR correct, PR incorrect, PR omitted, and median reaction time) were chosen as dependent variables. The Shapiro-Wilk test was used to determine data normality, while Levene's test was used to determine homogeneity. The Mann-Whitney U and Kruskal-Wallis tests were used to draw comparisons. For post-hoc comparison, Dunn's test with Holm correction was employed. The threshold for significance was set at p = 0.05. Results. The percentile rank of correct responses (p = 0.005) and median reaction time (p = 0.033) related to birth orders showed significant results. Other factors related to family information and reactive stress tolerance were not significant. Conclusions. According to the findings, it was concluded that being in new environment for a long duration reduced the early living experience related to family demography but effect of birth order still continued.

For-profit versus non-profit cybersecurity posture: breach types and locations in healthcare organisations.

The implementation of emerging technologies has resulted in an increase of data breaches in healthcare organisations, especially during the COVID-19 pandemic. Health information and cybersecurity managers need to understand if, and to what extent, breach types and locations are associated with their organisation's business type. To investigate if breach type and breach location are associated with business type, and if so, investigate how these factors affect information systems and protected health information in for-profit versus non-profit organisations. The quantitative study was performed using chi-square tests for association and post-hoc comparison of column proportions analysis on an archival data set of reported healthcare data breaches from 2020 to 2022. Data from the Department of Health and Human Services website was retrieved and each organisation classified as for-profit or non-profit. For-profit organisations experienced a significantly higher number of breaches due to theft, and non-profit organisations experienced a significantly higher number of breaches due to unauthorised access. Furthermore, the number of breaches that occurred on laptops and paper/films was significantly higher in for-profit organisations. While the threat level of hacking techniques is the same in for-profit and non-profit organisations, certain breach types are more likely to occur within specific breach locations based on the organisation's business type. To protect the privacy and security of medical information, health information and cybersecurity managers need to align with industry-leading frameworks and controls to prevent specific breach types that occur in specific locations within their environments.

Menopause Transition: A Cross-Sectional Evaluation on Muscle Size and Quality.

The menopause transition yields significant physiological alterations. The purpose was to characterize lean soft tissue (LST), muscle size (muscle cross-sectional area (mCSA)), muscle quality (echo intensity (EI)), and strength across the menopause transition. A secondary aim was to evaluate whole-body protein turnover in a subsample of women. Seventy-two healthy women were enrolled in this cross-sectional study based on menopause stage (PRE: n = 24; PERI: n = 24; POST: n = 24). Whole-body LST was measured via dual-energy x-ray absorptiometry, and muscle characteristics (mCSA and EI) were measured via B-mode ultrasound of the vastus lateralis. Maximal voluntary contractions (N·m) of the knee extensors were evaluated. Physical activity (in minutes per day) was accounted for using the International Physical Activity Questionnaire. A subsample of women ( n = 27) ingested 2.0 g of 15 N-alanine to determine whole-body net protein balance (NB; in grams per kilogram of body mass per day). Significant differences were evident in LST ( P = 0.022), leg LST ( P = 0.05), and EI ( P = 0.018) between menopause stages. Bonferroni post-hoc comparisons revealed greater LST in PRE versus PERI (mean difference (MD) ± SE, 3.8 ± 1.5 kg; P = 0.048) and POST (3.9 ± 1.5 lb; P = 0.049). Similarly, EI was significantly higher in PERI PRE (MD, 18.3 ± 7.1 a.u.; P = 0.036). There was no significant difference in mCSA ( P = 0.082) or in maximal voluntary contraction ( P = 0.167). NB was significantly different across groups ( P = 0.026); NB was greater in PRE compared with PERI (MD, 0.39 ± 0.17 g·kg -1 ; P = 0.090), and from PRE to POST (MD, 0.46 ± 0.17 g·kg -1 ; P = 0.042). Physical activity was not significantly different across groups but demonstrated a linear increase from PRE to POST. The current findings suggest that LST, muscle quality, and protein balance may be negatively influenced by the menopause transition.

Lateral abdominal muscle thickness during breathing maneuvers in women with and without stress urinary incontinence.

Stress urinary incontinence (SUI) patients predominantly experience involuntary leakage during respiratory functions that induce a rapid increase in intra-abdominal pressure (IAP) such as coughing and sneezing. The abdominal muscles have an important role in the forced expiration and modulation of IAP. We hypothesized that SUI patients have different thickness changes in the abdominal muscles compared to healthy individuals during breathing maneuvers. This case-control study was conducted on 17 adult women with SUI and 20 continent women. Muscle thickness changes were measured by ultrasonography at the end of deep inspiration and expiration, and the expiratory phase of voluntary coughing for external oblique (EO), internal oblique (IO), and transverse abdominis (TrA) muscles. The percent thickness changes of muscles were used and analyzed with a two-way mixed ANOVA test and post-hoc pairwise comparison at a confidence level of 95% (p < 0.05). The percent thickness changes of TrA muscle were significantly lower in SUI patients at deep expiration (p<0.001, Cohen's d=2.055) and coughing (p<0.001, Cohen's d=1.691). While, percent thickness changes for EO (p=0.004, Cohen's d=0.996) and IO thickness (p<0.001, Cohen's d=1.784) were greater at deep expiration and deep inspiration, respectively. The percent thickness changes of abdominal muscles differed between women with and without SUI during breathing maneuvers. The present study provided information regarding the altered function of abdominal muscles during breathing maneuvers; therefore, it is important to consider the respiratory role of abdominal muscles for the rehabilitation of SUI patients.

Designed acute physical activity to benefit primary school children’s cognition: Effects of cognitive challenge, bout duration and positive affect

Designed acute physical activity to benefit primary school children’s cognition: Effects of cognitive challenge, bout duration and positive affect

Reading and listening comprehension in Cantonese-speaking people with right hemisphere versus left hemisphere brain damage

ABSTRACT The problem in language comprehension in people with right hemisphere damage (RHD) is more equivocal than people with left hemisphere damage. This study explores the reading and listening comprehension of Cantonese-speaking individuals with RHD, left hemisphere damage, and neurotypical healthy controls using the Cantonese Computerized Revised Token Test (CRTT-Cantonese) adapted from the English CRTT. Eighteen native Cantonese-speaking individuals with RHD, 32 individuals with left hemisphere damage and aphasia (PWA), and 42 healthy controls participated in this study. All the participants completed the Cantonese Aphasia Battery, Hong Kong Oxford Cognitive Screen, the listening comprehension version of CRTT-Cantonese (CRTT-L-Cantonese), and the reading comprehension version of CRTT-Cantonese (CRTT-R-WF-Cantonese) across different sessions. Linear mixed-effect analysis revealed significant differences among the groups in CRTT-Cantonese tests. However, there were no significant difference between CRTT-L-Cantonese and CRTT-R-WF-Cantonese within the PWA, RHD and healthy control groups. Tukey post-hoc pairwise comparisons showed that PWA scored significantly lower than RHD and healthy control groups (p < 0.0001) in both CRTT-L-Cantonese and CRTT-R-WF-Cantonese, and the RHD group scored significantly lower than healthy control group only on the CRTT-R-WF-Cantonese. The results demonstrate that the CRTT-L-Cantonese and CRTT-R-WF-Cantonese differentiate language comprehension abilities among PWA, RHD and healthy control groups. Although the current findings did not show any diversion between reading and listening comprehension in RHD group, this group showed poorer performance in reading comprehension when compared to healthy controls. The latter findings may support the view that the right hemisphere contributes to reading comprehension in Chinese.

Optimization-Design and Atomization-Performance Study of Aerial Dual-Atomization Centrifugal Atomizer

The aerial atomizer is the most essential component of the plant protection UAV (unmanned aerial vehicle). However, the structural optimization of existing aerial atomizers lacks comprehensive consideration of spray parameters and structural parameters, and there is a shortage of available atomizer spray models, resulting in the unstable effect of UAV application. In our previous work, an aerial dual-atomization centrifugal atomizer was developed. In order to obtain an aerial atomizer with good atomization effect and its atomization model, structural optimization at different rotation speeds and flow rates of the atomizer, and its atomization performance, are studied in this paper. Firstly, with the droplet volume median diameter (VMD) and spectral width (SRW) as the evaluation index, through the single-factor, Plackett–Burman and Box–Behnken tests, the influence of rotation speed, flow rate, tooth number and tooth shape were studied. The regression models of the droplet VMD and SRW were established using multiple quadratic regression fitting of the test data. Secondly, in order to achieve the lowest droplet VMD and SRW, the response surface method and post-hoc multiple comparison method were used to obtain the optimized structure of the atomizer’s rotation ring at different rotation speeds (600–7000 r/min) and flow rates (500–1000 mL/min). Lastly, with the effective swath width (ESW) of the optimized atomizer as the evaluation index, through the Box–Behnken test, the influence of rotation speed, flow rate and spray height were studied. The multiple quadratic regression model of ESW was established with the test data. The test results indicated that rotation speed, flow rate and tooth number had a significant effect on droplet VMD and SRW; tooth shape had no significant effect on the droplet VMD and SRW, however, the square tooth shape had the best atomization effect; and rotation speed, flow rate and spray height had a significant effect on ESW. The optimized structural parameters were tooth shape: square, and tooth number: 20. The determination coefficient R2 of the regression model of VMD, SRW and ESW were 0.9976, 0.9770 and 0.9974, respectively, which indicates that the model was accurate, and can evaluate and predict the spray effect. This paper provides an optimized dual-atomization centrifugal atomizer, and its regression models of VMD, SRW and ESW for UAV applications can provide a reference for efficient UAV spraying.

Evaluation of the impact of COVID-19 on dairy cattle farming in Brazil

COVID-19 has brought numerous challenges for the dairy industry. This research aimed to analyze the impact of the COVID-19 pandemic on Brazilian dairy cattle farming. The study was conducted from December 2020 to February 2021 via Google Forms® platform. Data obtained were tabulated and evaluated using descriptive and multivariate analysis, Shapiro-Wilk, Kruskall-Wallis, Variance Analysis (ANOVA), and Dunn’s post-hoc comparison. The study was approved by the Committee for Ethics in Research with Human. In total, 73 viable responses were obtained, with participations from the Brazilian regions Midwest, Northeast, South, and Southeast. Among the biosecurity measures adopted during the study, 86% of farm employees began to sanitize their hands more often to prevent the transmission of COVID-19. Use of masks during work was required all the time during work by employees in 17 properties (23%), although the use of masks was not required in 40 (55%) farms and 16 properties required them during part of the work (22%). Regarding milk production, 99% of the respondents had no problems with milk collection and more than half of the producers had difficulties in acquiring animals. Moreover, thirty-seven percent of the interviewees said they were unaware of how the virus was transmitted. Regarding the size of the dairy farms, small farms were more likely to have no veterinary medical care. The pandemic brought positive and negative impacts to dairy properties, which was influenced by the number of animals, the presence of veterinary care, and average daily milk production.

Predicting and influencing PrEP use: the role of motivational and attitudinal factors

ABSTRACT This study examined PrEP attitude and HIV risk factors associated with PrEP motivation and evaluated the efficacy of a mock public health video that addresses both motivational and stigma issues for improving PrEP uptake over a standard educational video. Gay, bisexual, and other men who have sex with men (GBMSM; N = 604) were enrolled. One-way between subject analysis of variance (ANOVA) and post-hoc comparisons revealed that GBMSM in later stages of change had significantly higher endorsement of positive PrEP attitudes, PrEP stigma, objective and perceived HIV risk, HIV worry, and valued health benefits of PrEP more. Stepwise multiple regression revealed five significant predictors of PrEP motivation: HIV worry, objective HIV risk, anticipated PrEP stigma, positive PrEP attitudes, and perceived social consequences of PrEP use. Video conditions did not differ in their impact on PrEP attitudes or motivation; however, regardless of video condition, participants experienced a pre- to post-video increase in positive PrEP attitudes and motivation. Findings suggest HIV worry, objective HIV risk, positive PrEP attitudes, and perceived PrEP health benefits are important factors to optimize PrEP motivation, which can inform secondary prevention efforts. Further study is needed on promotional PrEP campaigns that disconfirm stigmatizing PrEP misconceptions.

Total Carbon Dioxide (TCO2) Concentrations in Thoroughbred and Quarter Racehorses in Louisiana.

The TCO2 (total carbon dioxide) test is performed on the blood of racehorses as a means of combatting the practice of administering alkalizing agents. This study evaluated serum TCO2 concentrations and factors influencing concentration of TCO2 in Thoroughbred and Quarter Horses. The normality of data were evaluated with a Shapiro-Wilk test. Mann-Whitney tests and Kruskal-Wallis tests were used against different effects. When a fixed effect was detected, Dunn's post-hoc comparisons were performed. The median pre-race serum TCO2 concentration (32.20 mmol/L (interquartile range (IQR): 30.80-33.50)) was higher than that of post-race samples (26.70 mmol/L (IQR: 24.55-29.25)) (P < .0001). The median TCO2 concentrations in pre-race samples were different between Thoroughbred (32.40 mmol/L (IQR: 30.90-33.60)) and Quarter Horses (31.30 mmol/L (IQR: 30.00-32.50)) (P < .0001). The median pre-race TCO2 concentrations were 32.75 (IQR: 31.40-33.90), 31.40 (IQR: 29.80-32.80), 32.50 (IQR: 31.20-33.88), and 31.60 (IQR 30.00-32.70) mmol/L in racehorses at Fair Grounds, Louisiana Downs, Delta Downs, and Evangeline Downs racetracks, respectively (P < .0001). The total serum TCO2 concentrations in Thoroughbred and Quarter Horse racehorses were affected by seasonal temperature variation (P < .0001). A smaller sample size was available for post-race samples (n=205) and Quarter Horse pre-race samples (n=351). The results of this study indicated that the breed, seasonal temperature variation, pre-race or post-race sampling, and track location are strongly correlated to total TCO2 concentrations. It was not clear whether the statistically significant differences in TCO2 levels among racetracks in Louisiana were due to location of racetracks and/or seasonal temperature variation.

A global perspective on collision and non-collision match characteristics in male rugby union: Comparisons by age and playing standard

ABSTRACT This study quantified and compared the collision and non-collision match characteristics across age categories (i.e. U12, U14, U16, U18, Senior) for both amateur and elite playing standards from Tier 1 rugby union nations (i.e. England, South Africa, New Zealand). Two-hundred and one male matches (5911 min ball-in-play) were coded using computerised notational analysis, including 193,708 match characteristics (e.g. 83,688 collisions, 33,052 tackles, 13,299 rucks, 1006 mauls, 2681 scrums, 2923 lineouts, 44,879 passes, 5568 kicks). Generalised linear mixed models with post-hoc comparisons and cluster analysis compared the match characteristics by age category and playing standard. Overall significant differences (p < 0.001) between age category and playing standard were found for the frequency of match characteristics, and tackle and ruck activity. The frequency of characteristics increased with age category and playing standard except for scrums and tries that were the lowest at the senior level. For the tackle, the percentage of successful tackles, frequency of active shoulder, sequential and simultaneous tackles increased with age and playing standard. For ruck activity, the number of attackers and defenders were lower in U18 and senior than younger age categories. Cluster analysis demonstrated clear differences in all and collision match characteristics and activity by age category and playing standard. These findings provide the most comprehensive quantification and comparison of collision and non-collision activity in rugby union demonstrating increased frequency and type of collision activity with increasing age and playing standard. These findings have implications for policy to ensure the safe development of rugby union players throughout the world.

Visual processing deficits in patients with schizophrenia spectrum and bipolar disorders and associations with psychotic symptoms, and intellectual abilities

ObjectiveLow-level sensory disruption is hypothesized as a precursor to clinical and cognitive symptoms in severe mental disorders. We compared visual discrimination performance in patients with schizophrenia spectrum disorder or bipolar disorder with healthy controls, and investigated associations with clinical symptoms and IQ. MethodsPatients with schizophrenia spectrum disorder (n = 32), bipolar disorder (n = 55) and healthy controls (n = 152) completed a computerized visual discrimination task. Participants responded whether the latter of two consecutive grids had higher or lower spatial frequency, and discrimination thresholds were estimated using an adaptive maximum likelihood procedure. Case-control differences in threshold were assessed using linear regression, F-test and post-hoc pair-wise comparisons. Linear models were used to test for associations between visual discrimination threshold and psychotic symptoms derived from the PANSS and IQ assessed using the Matrix Reasoning and Vocabulary subtests from the Wechsler Abbreviated Scale of Intelligence (WASI). ResultsRobust regression revealed a significant main effect of diagnosis on discrimination threshold (robust F = 6.76, p = .001). Post-hoc comparisons revealed that patients with a schizophrenia spectrum disorder (mean = 14%, SD = 0.08) had higher thresholds compared to healthy controls (mean = 10.8%, SD = 0.07, β = 0.35, t = 3.4, p = .002), as did patients with bipolar disorder (12.23%, SD = 0.07, β = 0.21, t = 2.42, p = .04). There was no significant difference between bipolar disorder and schizophrenia (β = −0.14, t = −1.2, p = .45). Linear models revealed negative associations between IQ and threshold across all participants when controlling for diagnostic group (β = −0.3, t = −3.43, p = .0007). This association was found within healthy controls (t = −3.72, p = .0003) and patients with bipolar disorder (t = −2.53, p = .015), and no significant group by IQ interaction on threshold (F = 0.044, p = .97). There were no significant associations between PANSS domain scores and discrimination threshold. ConclusionPatients with schizophrenia spectrum or bipolar disorders exhibited higher visual discrimination thresholds than healthy controls, supporting early visual deficits among patients with severe mental illness. Discrimination threshold was negatively associated with IQ among healthy controls and bipolar disorder patients. These findings elucidate perception-related disease mechanisms in severe mental illness, which warrants replication in independent samples.

Flexor-Pronator Mass Training Exercises Selectively Activate Forearm Musculature.

The flexor-pronator mass of the forearm contributes dynamic stability to the medial elbow. Training this muscle group is essential for overhead athletes, however, there is lack of evidence supporting use of training exercises. The purpose of this study was to measure the extent of EMG activity of the flexor pronator musculature during two distinct forearm strengthening exercises using resistance bands. It was hypothesized that two exercises would elicit at least moderate level of muscle activity, but the activation would be different in the pronator versus the flexor muscles. 10 healthy subjects (all males, age 36±12 years) were included. Surface EMG activity was measured on three muscles of the dominant-side forearm: flexor carpi ulnaris (FCP), flexor digitorum superficialis (FDS), and pronator teres (PT). After measurement of maximal voluntary contraction (MVC) for each muscle, subjects performed wrist ulnar deviation and forearm pronation exercises using elastic band resistance. Resistance was set to elicit moderate exertion (5/10 on the Borg CR10 scale). Order of exercise was randomized and three repetitions of each exercise were performed. Mean peak EMG activity in each muscle across repetitions during the eccentric phase of each exercise was calculated and expressed as a percentage of MVC. Moderate level of activity was defined as 21% of MVC or higher. Peak normalized EMG activity in each muscle was compared using two-way (exercise x muscle) repeated-measures ANOVA with post-hoc pairwise comparisons if a significant interaction was found. There was an exercise by muscle interaction effect (p<0.001). The ulnar deviation exercise selectively activated FCU (40.3%) versus FDS (19.5%, p=0.009) and PT (21.5%, p=0.022). Conversely, the pronation exercise selectively activated FDS (63.8%, p=0.002) and PT (73.0%, p=0.001) versus FDS (27.4%). The ulnar deviation and pronation exercises using elastic band resistance studied targeted and activated the flexor-pronator mass musculature. The ulnar deviation and pronation exercises using elastic band resistance are practical and effective means of training the flexor-pronator mass. These exercises can be readily prescribed to athletes and patients as part of their arm care program.

Effects of bilateral sequential theta-burst stimulation on 5-HT1A receptors in the dorsolateral prefrontal cortex in treatment-resistant depression: a proof-of-concept trial

Theta-burst stimulation (TBS) represents a brain stimulation technique effective for treatment-resistant depression (TRD) as underlined by meta-analyses. While the methodology undergoes constant refinement, bilateral stimulation of the dorsolateral prefrontal cortex (DLPFC) appears promising to restore left DLPFC hypoactivity and right hyperactivity found in depression. The post-synaptic inhibitory serotonin-1A (5-HT1A) receptor, also occurring in the DLPFC, might be involved in this mechanism of action. To test this hypothesis, we performed PET-imaging using the tracer [carbonyl-11C]WAY-100635 including arterial blood sampling before and after a three-week treatment with TBS in 11 TRD patients compared to sham stimulation (n = 8 and n = 3, respectively). Treatment groups were randomly assigned, and TBS protocol consisted of excitatory intermittent TBS to the left and inhibitory continuous TBS to the right DLPFC. A linear mixed model including group, hemisphere, time, and Hamilton Rating Scale for Depression (HAMD) score revealed a 3-way interaction effect of group, time, and HAMD on specific distribution volume (VS) of 5-HT1A receptor. While post-hoc comparisons showed no significant changes of 5-HT1A receptor VS in either group, higher 5-HT1A receptor VS after treatment correlated with greater difference in HAMD (r = −0.62). The results of this proof-of-concept trial hint towards potential effects of TBS on the distribution of the 5-HT1A receptor. Due to the small sample size, all results must, however, be regarded with caution.

Effect of Core Exercises on Motor Function Recovery in Stroke Survivors with Very Severe Motor Impairment

Paresis of the upper and lower limbs is a typical issue in stroke survivors. This study aims to determine whether core exercises help stroke survivors with very severe motor impairment recover their motor function. This study employed a within-subjects design. Eleven hemiparetic stroke patients with very severe motor impairment (FMA score < 35) and ages ranging from 24 to 52 years old were enrolled in this study. All participants engaged in supervised core exercise training twice a week for 12 weeks. The main outcome measures were Fugl-Meyer Assessment Lower Extremity (FMA-LE) and Fugl-Meyer Assessment Upper Extremity (FMA-UE), which were measured before training and at intervals of four weeks during training. Repeated measures ANOVA was used to analyze the effect of core exercises on motor function performance and lower extremity motor function and upper extremity motor function recovery. There were significant differences in the mean scores for motor function performance, lower extremity motor function, and upper extremity motor function throughout the four time points. A post-hoc pairwise comparison using the Bonferroni correction revealed that mean scores significantly increased and were statistically different between the initial assessment and follow-up assessments four, eight, and twelve weeks later. This study suggests that 12 weeks of core exercise training is effective for improving motor function recovery in patients with very severe motor impairment.

Characterising spinal cerebrospinal fluid flow in the pig with phase-contrast magnetic resonance imaging

BackgroundDetecting changes in pulsatile cerebrospinal fluid (CSF) flow may assist clinical management decisions, but spinal CSF flow is relatively understudied. Traumatic spinal cord injuries (SCI) often cause spinal cord swelling and subarachnoid space (SAS) obstruction, potentially causing pulsatile CSF flow changes. Pigs are emerging as a favoured large animal SCI model; therefore, the aim of this study was to characterise CSF flow along the healthy pig spine.MethodsPhase-contrast magnetic resonance images (PC-MRI), retrospectively cardiac gated, were acquired for fourteen laterally recumbent, anaesthetised and ventilated, female domestic pigs (22–29 kg). Axial images were obtained at C2/C3, T8/T9, T11/T12 and L1/L2. Dorsal and ventral SAS regions of interest (ROI) were manually segmented. CSF flow and velocity were determined throughout a cardiac cycle. Linear mixed-effects models, with post-hoc comparisons, were used to identify differences in peak systolic/diastolic flow, and maximum velocity (cranial/caudal), across spinal levels and dorsal/ventral SAS. Velocity wave speed from C2/C3 to L1/L2 was calculated.ResultsPC-MRI data were obtained for 11/14 animals. Pulsatile CSF flow was observed at all spinal levels. Peak systolic flow was greater at C2/C3 (dorsal: − 0.32 ± 0.14 mL/s, ventral: − 0.15 ± 0.13 mL/s) than T8/T9 dorsally (− 0.04 ± 0.03 mL/s; p < 0.001), but not different ventrally (− 0.08 ± 0.08 mL/s; p = 0.275), and no difference between thoracolumbar levels (p > 0.05). Peak diastolic flow was greater at C2/C3 (0.29 ± 0.08 mL/s) compared to T8/T9 (0.03 ± 0.03 mL/s, p < 0.001) dorsally, but not different ventrally (p = 1.000). Cranial and caudal maximum velocity at C2/C3 were greater than thoracolumbar levels dorsally (p < 0.001), and T8/T9 and L1/L2 ventrally (p = 0.022). Diastolic velocity wave speed was 1.41 ± 0.39 m/s dorsally and 1.22 ± 0.21 m/s ventrally, and systolic velocity wave speed was 1.02 ± 0.25 m/s dorsally and 0.91 ± 0.22 m/s ventrally.ConclusionsIn anaesthetised and ventilated domestic pigs, spinal CSF has lower pulsatile flow and slower velocity wave propagation, compared to humans. This study provides baseline CSF flow at spinal levels relevant for future SCI research in this animal model.

Natural and recycled aggregate concrete containing rice husk ash as replacement of cement: Mechanical properties, microstructure, strength model and statistical analysis

This study investigated the effects of the replacement ratio of natural aggregates (NAs) by recycled aggregate (RAs), the replacement ratio of cement by rice husk ash (RHA), and their interaction on compressive properties of recycled aggregate concrete (RAC). The RAs used consisted of 37.5 wt% recycled brick aggregates and 62.5 wt% recycled concrete aggregates from construction and demolition waste. To optimise the properties of RAC mixture, a full factorial design of experiment was applied in designing the concrete mixture proportion. The two factors considered were: the replacement ratio (wt.%) of RA with five levels (0%, 30%, 50%, 70% and 100%) and the replacement ratio (wt.%) of RHA with four levels (0%, 10%, 20% and 30%). Axial compression tests based on the full factorial experiment were conducted on cubic and prismatic samples at 28 days to evaluate their compressive properties. In addition, further cubic concrete groups were also tested in axial compression at different concrete ages (i.e., 3, 7, 28, 56 and 91 days) to understand the effect of concrete age on the compressive strength. Statistical analyses including ANOVA, post-hoc pairwise comparisons and effect size (Cohen's d) computation were performed to evaluate the experimental results. The results indicated that the compressive strength of concrete at 28 days was significantly affected by the replacement ratio of RA and RHA (both p-values <0.0001), as well as their interaction (p-value = 0.0001). For the four different replacement ratios (0%, 10%, 20% and 30%) of RHA considered in this study, the optimised ratio was 10%, which resulted in 0.4% and 4.9% increases in compressive strength of NAC and RAC (RA replacement ratio = 100%), respectively. RHA contributed more to the strength improvement of RAC than to that of NAC, both at 28 days and at 91 days. A new compressive strength model was developed for concrete containing RAs and RHA (or for other supplementary cementitious materials such as fly ash, silica fume, metakaolin and ground granulated blast slag). The validation with the experimental results from the literature showed a good accuracy of this model for concrete strength prediction, with 4.45% mean absolute percentage error and 2.3 root mean square error.

Bacterial contamination of multi-use antibiotic steroid eye ointments and drops.

This comprehensive prospective study aimed to investigate the bacterial contamination of antibiotic steroid eye ointments and drops frequently used by eye patients. In this comprehensive prospective study, a total of 410 multi-use topical eye medications containing 15 different ingredients from 22 pharmaceutical companies used by 185 patients were analyzed. Four groups were formed as follows: group 1: antibiotic ointments (n: 109); group 2: antibiotic drops (n: 103); group 3: steroid ointments (n: 67); and group 4: steroid drops (n: 131). Topical multi-use eye drops and ointments used by patients at home for at least 1week were randomly collected. The caps and contents were separately bacteriologically examined in a chocolate agar medium. Our study detected bacterial contamination in 23 containers (5.6%) of the total 410 topical drugs. According to the groups, bacterial contamination was detected in 10 of 67 (14.9%) steroid ointments, 6 of 109 (5.5%) antibiotic ointments, 4 of 131(3.1%) steroid drops, and 3 of 103 (2.9%) antibiotic drops. While the bacterial contamination rate in ointments was 9.1%, this rate was 3% in drops. The difference between them was statistically significant (p = 0.015). According to the post-hoc pairwise comparisons, the difference between steroid drops and steroid ointment (p = 0.0023) was statistically significant. Among all drugs, contamination was detected in 12 of the 93 (12.9%) containers used after keratitis, conjunctivitis, and inflammatory conditions. It was determined that preservatives statistically reduced bacterial growth on the cap. The preservatives did not have a statistically significant effect on the bacterial contamination of the contents compared to the caps. While all contaminations were detected in illiterate and primary school graduates, no contamination was seen in the drugs used by any secondary school or university graduate. Our study detected contamination in all topical ophthalmic drug groups. Contamination rates were found to be higher in ointments and steroids. Bacterial contamination was also seen in drugs containing preservatives. We should be careful in the use of topical medications. We do not recommend the bilateral use of ointments and drops in infected eyes, such as those with keratitis, or after intraocular surgeries, such as those for cataracts.

Neuropsychiatric Symptom Burden across Neurodegenerative Disorders and its Association with Function.

Neuropsychiatric symptoms (NPS) are prevalent in neurodegenerative disorders, however, their frequency and impact on function across different disorders is not well understood. We compared the frequency and severity of NPS across Alzheimer's disease (AD) (either with mild cognitive impairment or dementia), Cerebrovascular disease (CVD), Parkinson's disease (PD), frontotemporal dementia (FTD), and amyotrophic lateral sclerosis (ALS), and explored the association between NPS burden and function. We obtained data from Ontario Neurodegenerative Disease Research Initiative (ONDRI) that included following cohorts: AD (N = 111), CVD (N = 148), PD (N = 136), FTD (N = 50) and ALS (N = 36). We compared the frequency and severity of individual NPS (assessed by the neuropsychiatric inventory questionnaire) across cohorts using generalized estimating equations and analysis of variance. Second, we assessed the relationship of NPS burden with instrumental (iADLs) and basic (ADLs) activities of living across cohorts using multivariate linear regression while adjusting for relevant demographic and clinical covariates. Frequency of NPS varied across cohorts (χ2(4) = 34.4, p < .001), with post-hoc tests showing that FTD had the greatest frequency as compared to all other cohorts. The FTD cohort also had the greatest severity of NPS (H(4) = 34.5, p < .001). Further, there were differences among cohorts in terms of the association between NPS burden and ADLs (F(4,461) = 3.1, p = 0.02). Post-hoc comparisons suggested that this finding was driven by the FTD group, however, the differences did not remain significant following Bonferroni correction. There were no differences among cohorts in terms of the association between NPS burden and IADLs. NPS frequency and severity are markedly greater in FTD as compared to other neurodegenerative diseases. Further, NPS burden appears to be associated differently with function across neurodegenerative disorders, highlighting the need for individualized clinical interventions.